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Protein

Inhibitor of growth protein 2

Gene

Ing2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs) (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215Histone H3K4me3By similarity1
Metal bindingi216Zinc 1By similarity1
Metal bindingi218Zinc 1By similarity1
Binding sitei226Histone H3K4me3By similarity1
Metal bindingi229Zinc 2By similarity1
Binding sitei230Histone H3K4me3By similarity1
Metal bindingi234Zinc 2By similarity1
Binding sitei238Histone H3K4me3By similarity1
Metal bindingi240Zinc 1; via pros nitrogenBy similarity1
Metal bindingi243Zinc 1By similarity1
Metal bindingi256Zinc 2By similarity1
Metal bindingi259Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri213 – 262PHD-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

GO - Biological processi

  • chromatin modification Source: UniProtKB-KW
  • flagellated sperm motility Source: BHF-UCL
  • male germ-line stem cell asymmetric division Source: BHF-UCL
  • male meiosis I Source: BHF-UCL
  • negative regulation of apoptotic signaling pathway Source: MGI
  • negative regulation of cell proliferation Source: InterPro
  • positive regulation of histone deacetylation Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  • regulation of cellular senescence Source: InterPro
  • regulation of growth Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: MGI
  • seminiferous tubule development Source: BHF-UCL
  • spermatid development Source: BHF-UCL
  • spermatogenesis Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Growth regulation, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-6811555. PI5P Regulates TP53 Acetylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitor of growth protein 2
Alternative name(s):
Inhibitor of growth 1-like protein
p33ING2
Gene namesi
Name:Ing2
Synonyms:Ing1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1916510. Ing2.

Subcellular locationi

  • Nucleus By similarity

  • Note: Predominantly cytoplasmic. Localized to chromatin and nuclear matrix. Upon reduced PtdIns5P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002126641 – 281Inhibitor of growth protein 2Add BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki196Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity

Post-translational modificationi

Sumoylation enhances its association with SIN3A and is required for binding to some target gene promoters, this is the case for TMEM71.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9ESK4.
MaxQBiQ9ESK4.
PaxDbiQ9ESK4.
PeptideAtlasiQ9ESK4.
PRIDEiQ9ESK4.

PTM databases

PhosphoSitePlusiQ9ESK4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000063049.
CleanExiMM_ING2.
GenevisibleiQ9ESK4. MM.

Interactioni

Subunit structurei

Interacts with H3K4me3 and to a lesser extent with H3K4me2. Component of a mSin3A-like complex at least consisting of SIN3A, HDAC1, HDAC2, RBBP4/RbAp48, RBBP7/RbAp46, SAP30 and ING2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213322. 3 interactors.
DIPiDIP-38912N.
MINTiMINT-1340342.
STRINGi10090.ENSMUSP00000079226.

Structurei

Secondary structure

1281
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi210 – 212Combined sources3
Turni216 – 219Combined sources4
Beta strandi224 – 228Combined sources5
Beta strandi238 – 240Combined sources3
Helixi242 – 244Combined sources3
Helixi257 – 260Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WESNMR-A205-262[»]
2G6QX-ray2.00A205-264[»]
ProteinModelPortaliQ9ESK4.
SMRiQ9ESK4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ESK4.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 281PBRBy similarityAdd BLAST17

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili49 – 101Sequence analysisAdd BLAST53

Domaini

The PHD-type zinc finger mediates the binding to H3K4me3.By similarity
The polybasic region (PBR) is responsive to the binding to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns5P).By similarity

Sequence similaritiesi

Belongs to the ING family.Curated
Contains 1 PHD-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri213 – 262PHD-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1973. Eukaryota.
COG5034. LUCA.
GeneTreeiENSGT00550000074538.
HOGENOMiHOG000239724.
HOVERGENiHBG006607.
InParanoidiQ9ESK4.
KOiK19198.
OMAiLCHMTNG.
OrthoDBiEOG091G0J8Y.
TreeFamiTF352014.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR028639. ING2.
IPR028651. ING_fam.
IPR024610. ING_N_histone_binding.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10333. PTHR10333. 1 hit.
PTHR10333:SF37. PTHR10333:SF37. 1 hit.
PfamiPF12998. ING. 1 hit.
[Graphical view]
SMARTiSM01408. ING. 1 hit.
SM00249. PHD. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ESK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGQQQQQQL YSSAALLTGE RSRLLSCYVQ DYLECVESLP HDMQRNVSVL
60 70 80 90 100
RELDNKYQET LKEIDDVYEK YKKEDDSNQK KRLQQHLQRA LINSQELGDE
110 120 130 140 150
KIQIVTQMLE LVENRARQME LHSQCFQDPA ESERASDKSK MDSSQPERSS
160 170 180 190 200
RRPRRQRTSE SRDLCHMTNG IDDCDDQPPK EKRSKSAKKK KRSKAKQERE
210 220 230 240 250
ASPVEFAIDP NEPTYCLCNQ VSYGEMIGCD NEQCPIEWFH FSCVSLTYKP
260 270 280
KGKWYCPKCR GDNEKTMDKS TEKTKKERRA R
Length:281
Mass (Da):32,978
Last modified:October 3, 2012 - v3
Checksum:i6D953B52822C2D8A
GO

Sequence cautioni

The sequence AAH50003 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26S → T in AAG12173 (Ref. 1) Curated1
Sequence conflicti26S → T in AAH50003 (PubMed:15489334).Curated1
Sequence conflicti72K → E in AAG12173 (Ref. 1) Curated1
Sequence conflicti104I → N in AAG12173 (Ref. 1) Curated1
Sequence conflicti110E → G in AAG12173 (Ref. 1) Curated1
Sequence conflicti116A → S in AAG12173 (Ref. 1) Curated1
Sequence conflicti203P → S in AAG12173 (Ref. 1) Curated1
Sequence conflicti250P → H in AAG12173 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF078834 mRNA. Translation: AAG12173.1.
AK048800 mRNA. Translation: BAC33461.1.
AK083144 mRNA. Translation: BAC38783.1.
AC107236 Genomic DNA. No translation available.
CH466554 Genomic DNA. Translation: EDL35605.1.
BC096433 mRNA. Translation: AAH96433.1.
BC138229 mRNA. Translation: AAI38230.1.
BC050003 mRNA. Translation: AAH50003.1. Sequence problems.
CCDSiCCDS22298.1.
RefSeqiNP_075992.2. NM_023503.3.
UniGeneiMm.430733.

Genome annotation databases

EnsembliENSMUST00000080353; ENSMUSP00000079226; ENSMUSG00000063049.
GeneIDi69260.
KEGGimmu:69260.
UCSCiuc009lrf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF078834 mRNA. Translation: AAG12173.1.
AK048800 mRNA. Translation: BAC33461.1.
AK083144 mRNA. Translation: BAC38783.1.
AC107236 Genomic DNA. No translation available.
CH466554 Genomic DNA. Translation: EDL35605.1.
BC096433 mRNA. Translation: AAH96433.1.
BC138229 mRNA. Translation: AAI38230.1.
BC050003 mRNA. Translation: AAH50003.1. Sequence problems.
CCDSiCCDS22298.1.
RefSeqiNP_075992.2. NM_023503.3.
UniGeneiMm.430733.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WESNMR-A205-262[»]
2G6QX-ray2.00A205-264[»]
ProteinModelPortaliQ9ESK4.
SMRiQ9ESK4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213322. 3 interactors.
DIPiDIP-38912N.
MINTiMINT-1340342.
STRINGi10090.ENSMUSP00000079226.

PTM databases

PhosphoSitePlusiQ9ESK4.

Proteomic databases

EPDiQ9ESK4.
MaxQBiQ9ESK4.
PaxDbiQ9ESK4.
PeptideAtlasiQ9ESK4.
PRIDEiQ9ESK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000080353; ENSMUSP00000079226; ENSMUSG00000063049.
GeneIDi69260.
KEGGimmu:69260.
UCSCiuc009lrf.2. mouse.

Organism-specific databases

CTDi3622.
MGIiMGI:1916510. Ing2.

Phylogenomic databases

eggNOGiKOG1973. Eukaryota.
COG5034. LUCA.
GeneTreeiENSGT00550000074538.
HOGENOMiHOG000239724.
HOVERGENiHBG006607.
InParanoidiQ9ESK4.
KOiK19198.
OMAiLCHMTNG.
OrthoDBiEOG091G0J8Y.
TreeFamiTF352014.

Enzyme and pathway databases

ReactomeiR-MMU-6811555. PI5P Regulates TP53 Acetylation.

Miscellaneous databases

EvolutionaryTraceiQ9ESK4.
PROiQ9ESK4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000063049.
CleanExiMM_ING2.
GenevisibleiQ9ESK4. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR028639. ING2.
IPR028651. ING_fam.
IPR024610. ING_N_histone_binding.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR10333. PTHR10333. 1 hit.
PTHR10333:SF37. PTHR10333:SF37. 1 hit.
PfamiPF12998. ING. 1 hit.
[Graphical view]
SMARTiSM01408. ING. 1 hit.
SM00249. PHD. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiING2_MOUSE
AccessioniPrimary (citable) accession number: Q9ESK4
Secondary accession number(s): Q4VAD7, Q80VI5, Q8BGU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: October 3, 2012
Last modified: November 30, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.