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Protein

Inhibitor of growth protein 2

Gene

Ing2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs) (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215Histone H3K4me3By similarity1
Metal bindingi216Zinc 1By similarity1
Metal bindingi218Zinc 1By similarity1
Binding sitei226Histone H3K4me3By similarity1
Metal bindingi229Zinc 2By similarity1
Binding sitei230Histone H3K4me3By similarity1
Metal bindingi234Zinc 2By similarity1
Binding sitei238Histone H3K4me3By similarity1
Metal bindingi240Zinc 1; via pros nitrogenBy similarity1
Metal bindingi243Zinc 1By similarity1
Metal bindingi256Zinc 2By similarity1
Metal bindingi259Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri213 – 262PHD-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

GO - Biological processi

  • chromatin organization Source: UniProtKB-KW
  • flagellated sperm motility Source: BHF-UCL
  • male germ-line stem cell asymmetric division Source: BHF-UCL
  • male meiosis I Source: BHF-UCL
  • negative regulation of apoptotic signaling pathway Source: MGI
  • negative regulation of cell proliferation Source: InterPro
  • positive regulation of histone deacetylation Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transforming growth factor beta receptor signaling pathway Source: MGI
  • regulation of cellular senescence Source: InterPro
  • regulation of growth Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: MGI
  • seminiferous tubule development Source: BHF-UCL
  • spermatid development Source: BHF-UCL
  • spermatogenesis Source: BHF-UCL
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChromatin regulator
Biological processGrowth regulation, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-6811555 PI5P Regulates TP53 Acetylation

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitor of growth protein 2
Alternative name(s):
Inhibitor of growth 1-like protein
p33ING2
Gene namesi
Name:Ing2
Synonyms:Ing1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1916510 Ing2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002126641 – 281Inhibitor of growth protein 2Add BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki196Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity

Post-translational modificationi

Sumoylation enhances its association with SIN3A and is required for binding to some target gene promoters, this is the case for TMEM71.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9ESK4
MaxQBiQ9ESK4
PaxDbiQ9ESK4
PeptideAtlasiQ9ESK4
PRIDEiQ9ESK4

PTM databases

PhosphoSitePlusiQ9ESK4

Expressioni

Gene expression databases

BgeeiENSMUSG00000063049
CleanExiMM_ING2
GenevisibleiQ9ESK4 MM

Interactioni

Subunit structurei

Interacts with H3K4me3 and to a lesser extent with H3K4me2. Component of a mSin3A-like complex at least consisting of SIN3A, HDAC1, HDAC2, RBBP4/RbAp48, RBBP7/RbAp46, SAP30 and ING2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213322, 3 interactors
DIPiDIP-38912N
IntActiQ9ESK4, 2 interactors
MINTiQ9ESK4
STRINGi10090.ENSMUSP00000079226

Structurei

Secondary structure

1281
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi210 – 212Combined sources3
Turni216 – 219Combined sources4
Beta strandi224 – 228Combined sources5
Beta strandi238 – 240Combined sources3
Helixi242 – 244Combined sources3
Helixi257 – 260Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WESNMR-A205-262[»]
2G6QX-ray2.00A205-264[»]
ProteinModelPortaliQ9ESK4
SMRiQ9ESK4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ESK4

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 281PBRBy similarityAdd BLAST17

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili49 – 101Sequence analysisAdd BLAST53

Domaini

The PHD-type zinc finger mediates the binding to H3K4me3.By similarity
The polybasic region (PBR) is responsive to the binding to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns5P).By similarity

Sequence similaritiesi

Belongs to the ING family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri213 – 262PHD-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG1973 Eukaryota
COG5034 LUCA
GeneTreeiENSGT00550000074538
HOGENOMiHOG000239724
HOVERGENiHBG006607
InParanoidiQ9ESK4
KOiK19198
OMAiLCHMTNG
OrthoDBiEOG091G0J8Y
TreeFamiTF352014

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR028639 ING2
IPR024610 ING_N_histone_binding
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR10333:SF37 PTHR10333:SF37, 1 hit
PfamiView protein in Pfam
PF12998 ING, 1 hit
SMARTiView protein in SMART
SM01408 ING, 1 hit
SM00249 PHD, 1 hit
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ESK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGQQQQQQL YSSAALLTGE RSRLLSCYVQ DYLECVESLP HDMQRNVSVL
60 70 80 90 100
RELDNKYQET LKEIDDVYEK YKKEDDSNQK KRLQQHLQRA LINSQELGDE
110 120 130 140 150
KIQIVTQMLE LVENRARQME LHSQCFQDPA ESERASDKSK MDSSQPERSS
160 170 180 190 200
RRPRRQRTSE SRDLCHMTNG IDDCDDQPPK EKRSKSAKKK KRSKAKQERE
210 220 230 240 250
ASPVEFAIDP NEPTYCLCNQ VSYGEMIGCD NEQCPIEWFH FSCVSLTYKP
260 270 280
KGKWYCPKCR GDNEKTMDKS TEKTKKERRA R
Length:281
Mass (Da):32,978
Last modified:October 3, 2012 - v3
Checksum:i6D953B52822C2D8A
GO

Sequence cautioni

The sequence AAH50003 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26S → T in AAG12173 (Ref. 1) Curated1
Sequence conflicti26S → T in AAH50003 (PubMed:15489334).Curated1
Sequence conflicti72K → E in AAG12173 (Ref. 1) Curated1
Sequence conflicti104I → N in AAG12173 (Ref. 1) Curated1
Sequence conflicti110E → G in AAG12173 (Ref. 1) Curated1
Sequence conflicti116A → S in AAG12173 (Ref. 1) Curated1
Sequence conflicti203P → S in AAG12173 (Ref. 1) Curated1
Sequence conflicti250P → H in AAG12173 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF078834 mRNA Translation: AAG12173.1
AK048800 mRNA Translation: BAC33461.1
AK083144 mRNA Translation: BAC38783.1
AC107236 Genomic DNA No translation available.
CH466554 Genomic DNA Translation: EDL35605.1
BC096433 mRNA Translation: AAH96433.1
BC138229 mRNA Translation: AAI38230.1
BC050003 mRNA Translation: AAH50003.1 Sequence problems.
CCDSiCCDS22298.1
RefSeqiNP_075992.2, NM_023503.3
UniGeneiMm.430733

Genome annotation databases

EnsembliENSMUST00000080353; ENSMUSP00000079226; ENSMUSG00000063049
GeneIDi69260
KEGGimmu:69260
UCSCiuc009lrf.2 mouse

Similar proteinsi

Entry informationi

Entry nameiING2_MOUSE
AccessioniPrimary (citable) accession number: Q9ESK4
Secondary accession number(s): Q4VAD7, Q80VI5, Q8BGU8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: October 3, 2012
Last modified: April 25, 2018
This is version 151 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health