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Protein

Calpain-10

Gene

Capn10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. May play a role in insulin-stimulated glucose uptake (By similarity).By similarity

Catalytic activityi

Broad endopeptidase specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731By similarity
Active sitei238 – 2381By similarity
Active sitei263 – 2631By similarity

GO - Molecular functioni

  1. calcium-dependent cysteine-type endopeptidase activity Source: MGI

GO - Biological processi

  1. actin cytoskeleton reorganization Source: BHF-UCL
  2. cellular response to insulin stimulus Source: BHF-UCL
  3. positive regulation of glucose import Source: BHF-UCL
  4. positive regulation of insulin secretion Source: MGI
  5. positive regulation of intracellular transport Source: BHF-UCL
  6. positive regulation of type B pancreatic cell apoptotic process Source: MGI
  7. proteolysis Source: MGI
  8. type B pancreatic cell apoptotic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Protein family/group databases

MEROPSiC02.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-10 (EC:3.4.22.-)
Alternative name(s):
Calcium-activated neutral proteinase 10
Short name:
CANP 10
Gene namesi
Name:Capn10
Synonyms:Capn8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1344392. Capn10.

Subcellular locationi

GO - Cellular componenti

  1. cell Source: MGI
  2. cytoplasm Source: GO_Central
  3. cytosol Source: MGI
  4. mitochondrion Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 666666Calpain-10PRO_0000207727Add
BLAST

Proteomic databases

PaxDbiQ9ESK3.
PRIDEiQ9ESK3.

PTM databases

PhosphoSiteiQ9ESK3.

Expressioni

Gene expression databases

BgeeiQ9ESK3.
CleanExiMM_CAPN10.
MM_CAPN8.
ExpressionAtlasiQ9ESK3. baseline and differential.
GenevestigatoriQ9ESK3.

Structurei

3D structure databases

ProteinModelPortaliQ9ESK3.
SMRiQ9ESK3. Positions 10-484, 508-632.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 321309Calpain catalyticPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni322 – 488167Domain III 1Add
BLAST
Regioni507 – 648142Domain III 2Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG313372.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000081807.
HOVERGENiHBG050787.
InParanoidiQ9ESK3.
KOiK08579.
OMAiSCVPHRY.
OrthoDBiEOG7RV9FM.
PhylomeDBiQ9ESK3.
TreeFamiTF314748.

Family and domain databases

InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR028791. CAPN10.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF30. PTHR10183:SF30. 1 hit.
PfamiPF01067. Calpain_III. 2 hits.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 2 hits.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 2 hits.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ESK3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAVRAETPA RELFRDAAFP ASDSSLFYNL STPLAQFRED ITWRRPQEIC
60 70 80 90 100
ATPQLFPDNP WEGQVKQGLL GDCWFLCACA ALQKSQHLLD QVFPPGQPGW
110 120 130 140 150
SDQKYQGFFT CRIWQFGHWE EVTIDDRLPC LAGRLCFSRC QREDVFWLPL
160 170 180 190 200
LEKAYAKVHG SYEHLWAGQV ADALVDLTGS LAERWSLKDV TKASGQQDRP
210 220 230 240 250
SGGEHRTCRQ LLHLKDRCLI SCSVLSPRAG ARELGEFHAF IISDLQELRS
260 270 280 290 300
QTGQGILLLR IHNPWGRRCW QGLWREGGEG WNQVEPAKES ELLAQLQEGE
310 320 330 340 350
FWVEEEEFLR EFDEVTIGYP VTEAGHLQSL HTERVLCHTR TLPGAWVTGQ
360 370 380 390 400
SAGGCRNNSC FPCNPKFWLR LLEPSEVCVA VLQRPRRRLV GQTRALAGAS
410 420 430 440 450
PAPVNLPGKD YQAVGLHIWK VEKRKISLPR VLSAPPVAGT ACHAYDREIH
460 470 480 490 500
LRCELSPGYY LAVPSTFLKD VPGQFLLRVF STGKISLSAV RLATKGASPG
510 520 530 540 550
TALPAGEWET VQLQGCWRAG QTAGGSRNFA SYPCNPCLPF SVPEGAGPRY
560 570 580 590 600
IRITLQQHCR LSDSQLHPIG FHVFQVPADG ENQDACSLLL QEPLLSCVPH
610 620 630 640 650
RYAQEVSRLC LLSVGNYRIV PSTYLPDTEG TFTVTIATRI DRQSIHSQEM
660
LGQLLQEVSF MAVMKA
Length:666
Mass (Da):74,596
Last modified:February 12, 2003 - v2
Checksum:iA0B1B37EE1DE2371
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti481 – 4811S → F (PubMed:11017071).Curated
Sequence conflicti481 – 4811S → F (Ref. 2) Curated
Sequence conflicti602 – 6054YAQE → TPRK (Ref. 3) Curated
Sequence conflicti663 – 6631V → E in BAC40211 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF089089 mRNA. Translation: AAG17967.1.
AF203031 Genomic DNA. Translation: AAL54919.1.
AF126867 mRNA. Translation: AAD41779.1.
AK088207 mRNA. Translation: BAC40211.1.
BC005681 mRNA. Translation: AAH05681.1.
BC010969 mRNA. Translation: AAH10969.1.
CCDSiCCDS15181.1.
RefSeqiNP_035926.2. NM_011796.2.
UniGeneiMm.294315.

Genome annotation databases

EnsembliENSMUST00000027488; ENSMUSP00000027488; ENSMUSG00000026270.
GeneIDi23830.
KEGGimmu:23830.
UCSCiuc007cby.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF089089 mRNA. Translation: AAG17967.1.
AF203031 Genomic DNA. Translation: AAL54919.1.
AF126867 mRNA. Translation: AAD41779.1.
AK088207 mRNA. Translation: BAC40211.1.
BC005681 mRNA. Translation: AAH05681.1.
BC010969 mRNA. Translation: AAH10969.1.
CCDSiCCDS15181.1.
RefSeqiNP_035926.2. NM_011796.2.
UniGeneiMm.294315.

3D structure databases

ProteinModelPortaliQ9ESK3.
SMRiQ9ESK3. Positions 10-484, 508-632.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC02.018.

PTM databases

PhosphoSiteiQ9ESK3.

Proteomic databases

PaxDbiQ9ESK3.
PRIDEiQ9ESK3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027488; ENSMUSP00000027488; ENSMUSG00000026270.
GeneIDi23830.
KEGGimmu:23830.
UCSCiuc007cby.1. mouse.

Organism-specific databases

CTDi11132.
MGIiMGI:1344392. Capn10.

Phylogenomic databases

eggNOGiNOG313372.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000081807.
HOVERGENiHBG050787.
InParanoidiQ9ESK3.
KOiK08579.
OMAiSCVPHRY.
OrthoDBiEOG7RV9FM.
PhylomeDBiQ9ESK3.
TreeFamiTF314748.

Miscellaneous databases

NextBioi303493.
PROiQ9ESK3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9ESK3.
CleanExiMM_CAPN10.
MM_CAPN8.
ExpressionAtlasiQ9ESK3. baseline and differential.
GenevestigatoriQ9ESK3.

Family and domain databases

InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR028791. CAPN10.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF30. PTHR10183:SF30. 1 hit.
PfamiPF01067. Calpain_III. 2 hits.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 2 hits.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 2 hits.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning of mouse protease gene."
    Horikawa Y., Ye H., Bell G.I.
    Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 129/SvJ.
  3. "Identification of a new calpain-like cDNA in mouse lung."
    Braun C., Seifert M., Engel M., Welter C.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Lung.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
    Tissue: Thymus.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiCAN10_MOUSE
AccessioniPrimary (citable) accession number: Q9ESK3
Secondary accession number(s): Q99J13, Q9WVF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: February 12, 2003
Last modified: February 4, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.