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Protein

NCK-interacting protein with SH3 domain

Gene

Nckipsd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1) (By similarity). Induces microspike formation, in vivo. In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42. May play an important role in the maintenance of sarcomere and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion.By similarity

GO - Molecular functioni

GO - Biological processi

  • positive regulation of neuron projection development Source: CACAO

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-5663213 RHO GTPases Activate WASPs and WAVEs

Names & Taxonomyi

Protein namesi
Recommended name:
NCK-interacting protein with SH3 domain
Alternative name(s):
54 kDa VacA-interacting protein
Short name:
VIP54
90 kDa N-WASP-interacting protein
90 kDa SH3 protein interacting with Nck
SH3 adapter protein SPIN90
WASP-interacting SH3-domain protein
Short name:
WISH
Wiskott-Aldrich syndrome protein-binding protein
Short name:
N-WASP-binding protein
Gene namesi
Name:Nckipsd
Synonyms:Spin90, Wasbp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1931834 Nckipsd

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000721311 – 714NCK-interacting protein with SH3 domainAdd BLAST714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei120PhosphoserineCombined sources1
Modified residuei174PhosphothreonineBy similarity1
Modified residuei260PhosphoserineCombined sources1
Modified residuei286PhosphoserineCombined sources1
Modified residuei673PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9ESJ4
MaxQBiQ9ESJ4
PaxDbiQ9ESJ4
PeptideAtlasiQ9ESJ4
PRIDEiQ9ESJ4

PTM databases

iPTMnetiQ9ESJ4
PhosphoSitePlusiQ9ESJ4

Expressioni

Gene expression databases

BgeeiENSMUSG00000032598
CleanExiMM_NCKIPSD
ExpressionAtlasiQ9ESJ4 baseline and differential
GenevisibleiQ9ESJ4 MM

Interactioni

Subunit structurei

Associates with the intermediate filaments, vimentin and desmin (By similarity). Binds the first and third SH3 domains of NCK (By similarity). Binds the proline-rich domains of N-WASP through its SH3 domain. Similarly, binds diaphanous protein homolog 1 (DRF1) (By similarity). Binds the SH3 domains of GRB2 through its proline-rich domains. Interacts with FASLG (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi219860, 1 interactor
IntActiQ9ESJ4, 9 interactors
MINTiQ9ESJ4
STRINGi10090.ENSMUSP00000035218

Structurei

3D structure databases

ProteinModelPortaliQ9ESJ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 58SH3PROSITE-ProRule annotationAdd BLAST58

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi168 – 185Nuclear localization signalSequence analysisAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi200 – 233Ser-richAdd BLAST34
Compositional biasi225 – 264Pro-richAdd BLAST40
Compositional biasi434 – 479Leu-richAdd BLAST46
Compositional biasi526 – 593Leu-richAdd BLAST68

Keywords - Domaini

SH3 domain, SH3-binding

Phylogenomic databases

eggNOGiKOG4035 Eukaryota
ENOG410Y8WT LUCA
GeneTreeiENSGT00390000015725
HOGENOMiHOG000008184
HOVERGENiHBG061630
InParanoidiQ9ESJ4
OMAiYHTDMMA
OrthoDBiEOG091G0D4N
PhylomeDBiQ9ESJ4
TreeFamiTF324522

Family and domain databases

CDDicd11849 SH3_SPIN90, 1 hit
InterProiView protein in InterPro
IPR018556 DUF2013
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR035514 SPIN90_SH3
PfamiView protein in Pfam
PF09431 DUF2013, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00326 SH3, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ESJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRALYAFRS AEPNAMAFAA GETFLVLERS STHWWLAARA RSGETGYVPP
60 70 80 90 100
AYLHRLQGME QDVLQAIDRA IEAVHNTAMR DGGKYSLEQR GVLQKLIHHR
110 120 130 140 150
KETLSRRGTS ASSATVMTPS TSDHHLDAAV SRQPNGVCRT GFERQHSLPS
160 170 180 190 200
SEHLGTDGAL YQVPPQPRRA APTTPPPPVK RRDREALVIS GSGGRTAIPS
210 220 230 240 250
GGSSVSSGSS ASSTSMDTLY TGSSPSELGP SCSPTPPPVP RRGAHTTVSQ
260 270 280 290 300
PQPSPSKAPS PEPPTEEVAA ETNSTPDDLE AQDALSPETT EEKAAAETVV
310 320 330 340 350
PRTIGAELME LVRRNTGLSH ELCRVAIGVV VGHIQATVPA SSPIMEQVLL
360 370 380 390 400
SLVEGKDLST ALPSGQVCHD QQRLEVIFAD LARRKDDAQQ RSWALYEDED
410 420 430 440 450
VIRCYLEELL HILTDADPEV CKKMCKRSDF ESVLALVAYY QMEHRASLRL
460 470 480 490 500
LLLKCFGAMC SLDAAIISTL VSSVLPVELA RDMQTDTQDH QKLCYSALVL
510 520 530 540 550
AMVFSMGEAV PYAHYEHLGT PFAQFLLSIV EDGLPMDTTE QLPDLCMNLL
560 570 580 590 600
LALNLHLTAP EQNVIMAALS RHTNVKIFSE KLLLLLNRGD DPVRIFRHEP
610 620 630 640 650
QPPHSVLKFL QDVFSSSATA AIFYHTDMMA LIDITVRQIA DLSPGDKLRM
660 670 680 690 700
EYLSLMHAVV RSTPYLQHRH RLSDLQATLR RILTEEEASP QCQMDRMIVQ
710
EMYKEFPDLG EVPS
Length:714
Mass (Da):78,572
Last modified:January 4, 2005 - v2
Checksum:i2111AB09799F4718
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF130313 mRNA Translation: AAF36503.2
AK134725 mRNA Translation: BAE22259.1
CH466560 Genomic DNA Translation: EDL21315.1
BC064818 mRNA Translation: AAH64818.1
CCDSiCCDS23538.1
RefSeqiNP_109654.2, NM_030729.4
UniGeneiMm.192416

Genome annotation databases

EnsembliENSMUST00000035218; ENSMUSP00000035218; ENSMUSG00000032598
ENSMUST00000202637; ENSMUSP00000144069; ENSMUSG00000107055
GeneIDi80987
KEGGimmu:80987
UCSCiuc009rqz.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSPN90_MOUSE
AccessioniPrimary (citable) accession number: Q9ESJ4
Secondary accession number(s): Q3UYF3, Q68G72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: January 4, 2005
Last modified: March 28, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health