Q9ESJ0 (XPO4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exportin-4
Short name=Exp4

Gene names

Name:	Xpo4
Synonyms:	Kiaa1721

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1151 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates the nuclear export of proteins (cargos) with broad substrate specificity. In the nucleus binds cooperatively to its cargo and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. XPO4 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus By similarity.
Subunit structure	Found in a complex with XPO4, Ran and EIF5A. Found in a complex with XPO4, Ran and SMAD3. Interacts with SMAD3. Interacts with Ran and cargo proteins in a GTP-dependent manner By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Shuttles between the nucleus and the cytoplasm By similarity.
Sequence similarities	Belongs to the exportin family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of protein export from nucleus Inferred from electronic annotation. Source: Compara protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1151	1151	Exportin-4		PRO_0000204712
Amino acid modifications
	Modified residue	464	1	Phosphoserine By similarity
	Modified residue	521	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	242	1	K → N in AAG09133. Ref.1
	Sequence conflict	242	1	K → N in BAC98240. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9ESJ0 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 91428FC24FB54628	FASTA	1,151	129,979
	10 20 30 40 50 60 MMAAALGPPE VIAQLENAAK VLMAPPSMVS NEQRQHAEHI FLSFRKSKSP FAVCRHILET 70 80 90 100 110 120 SKVDYVLFQA ATAIMEAVVR EWVLLEKGSI ESLRTFLLTY VLQRPNLQKY VREQILLAVA 130 140 150 160 170 180 VIVKRGSLDK SIDCKSIFHE VSQLISSGNP TVQTLACSIL TALLSEFSSS SKTSNIGLSM 190 200 210 220 230 240 EFHGNCKRVF QEEDLRQIFM LTVGVLQEFS RRENLSAQMS SVFQRYLALA NQVLSWNFLP 250 260 270 280 290 300 PKLGRHYIAM FESSQNVLLK PTESWREALL DSRVMELFFT VHRKIREDSD MAQDSLQCLA 310 320 330 340 350 360 QLASLHGPIF PDEGSQVDYL AHFIEGLLNT INGIEIEDSE AVGISSIISN LITVFPRNVL 370 380 390 400 410 420 TAIPSELFSS FVNCLTHLTC SFGRSAALEE VLDKDDMVYM EAYDKLLESW LTLVRDDKHF 430 440 450 460 470 480 HKGFFTQHAV QVFNSYIQCH LAAPDGTRNL TANGVASREE EEISELQEDD RDQFSDQLAS 490 500 510 520 530 540 VGMLGRIAAE HCMPLLTSLL EERVTRLHGQ LQRHQQQFLA SPGSSTIDNK MLDDLYEDIH 550 560 570 580 590 600 WLILVTGYLL ADDTQGETPL IPPEIMEYSI KHSSEVDINT TLQILGSPGE KASSIPGYSR 610 620 630 640 650 660 TDSVIRLLSA VLRVSEVESR AIRADLTHLL SPQMGKDIVW FLKRWAKTYL LVDEKLYDQI 670 680 690 700 710 720 SLPLSTAFGA DTEGSQWIIG YLLQKVISNL SVWSSEQDLA NDTVQLLVTL VERRERANLV 730 740 750 760 770 780 IQCENWWNLA KQFASRSPPL NFLSSPVQRT LMKALVLGGF AHMDTETKQQ YWTEVLQPLQ 790 800 810 820 830 840 QRFLRVINQE NFQQMCQQEE VKQEITATLE ALCGIAEATQ IDNVAILFNF LMDFLNNCIG 850 860 870 880 890 900 LMEVYKNTPE TVNLIIEVFV EVAHKQICYL GESKAMHLYE ACLTLLQVYS KNNLGRQRID 910 920 930 940 950 960 VTAEEEQYQD LLLIMELLTN LLSKEFIDFS DTDEVFRGHE PGQAAGRSVS AADVVLYGVN 970 980 990 1000 1010 1020 LILPLMSQDL LKFPTLCNQY YKLITFICEI FPEKIPQLPE DLFKSLMYSL ELGMTSMSSE 1030 1040 1050 1060 1070 1080 VCQLCLEALT PLAEQCAKAQ ETDSPLFLAT RHFLKLVFDM LVLQKHNTEM TTAAGEAFYT 1090 1100 1110 1120 1130 1140 LVCLHQAEYS ELVETLLSSQ QDPVIYQRLA DAFNKLTASS TPPALDRKQK MAFLKSLEEF 1150 MANVGGLLCV K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Exportin 4: a mediator of a novel nuclear export pathway in higher eukaryotes." Lipowsky G., Bischoff F.R., Schwarzmaier P., Kraft R., Kostka S., Hartmann E., Kutay U., Goerlich D. EMBO J. 19:4362-4371(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-1151. Tissue: Embryonic tail.
[4]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-521, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF145021 mRNA. Translation: AAG09133.1. AC154504 Genomic DNA. No translation available. AC154675 Genomic DNA. No translation available. AK129430 mRNA. Translation: BAC98240.1.
IPI	IPI00331063.
RefSeq	NP_065252.1. NM_020506.1.
UniGene	Mm.202747.
3D structure databases
ProteinModelPortal	Q9ESJ0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ESJ0. 1 interaction.
STRING	10090.ENSMUSP00000086909.
PTM databases
PhosphoSite	Q9ESJ0.
Proteomic databases
PaxDb	Q9ESJ0.
PRIDE	Q9ESJ0.
Protocols and materials databases
DNASU	57258.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000089482; ENSMUSP00000086909; ENSMUSG00000021952. ENSMUST00000174545; ENSMUSP00000133280; ENSMUSG00000021952.
GeneID	57258.
KEGG	mmu:57258.
UCSC	uc007udf.1. mouse.
Organism-specific databases
CTD	64328.
MGI	MGI:1888526. Xpo4.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG273883.
GeneTree	ENSGT00530000063137.
HOGENOM	HOG000154878.
HOVERGEN	HBG023214.
InParanoid	Q9ESJ0.
OrthoDB	EOG4Z36CX.
Gene expression databases
ArrayExpress	Q9ESJ0.
Bgee	Q9ESJ0.
CleanEx	MM_XPO4.
Genevestigator	Q9ESJ0.
GermOnline	ENSMUSG00000021952. Mus musculus.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR014877. CRM1_C_dom. [Graphical view]
Pfam	PF08767. CRM1_C. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	313565.
SOURCE	Search...

Entry information

Entry name

XPO4_MOUSE

Accession

Primary (citable) accession number: Q9ESJ0
Secondary accession number(s): E9QLH3, Q6ZPJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program