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Protein

Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase

Gene

Pkmyt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Negatively regulated by hyperphosphorylation during mitosis. The hyperphosphorylated form does not associate with CCNB1-CDC2 complexes. The PLK1 protein kinase may be required for mitotic phosphorylation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei130ATPPROSITE-ProRule annotation1
Active sitei224Proton acceptorPROSITE-ProRule annotation1
Metal bindingi229Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi242Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi107 – 115ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-156711. Polo-like kinase mediated events.
R-MMU-69273. Cyclin A/B1 associated events during G2/M transition.
R-MMU-69478. G2/M DNA replication checkpoint.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase (EC:2.7.11.1)
Alternative name(s):
Myt1 kinase
Gene namesi
Name:Pkmyt1
Synonyms:Myt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2137630. Pkmyt1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • Golgi membrane Source: UniProtKB-SubCell
  • membrane Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865741 – 490Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinaseAdd BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphothreonineBy similarity1
Modified residuei31PhosphoserineBy similarity1
Modified residuei85PhosphoserineBy similarity1
Modified residuei111PhosphoserineBy similarity1
Modified residuei134PhosphoserineBy similarity1
Modified residuei417Phosphothreonine; by PLK1By similarity1
Modified residuei464PhosphoserineBy similarity1
Modified residuei486Phosphoserine; by PLK1By similarity1

Post-translational modificationi

Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9ESG9.
PaxDbiQ9ESG9.
PeptideAtlasiQ9ESG9.
PRIDEiQ9ESG9.

PTM databases

iPTMnetiQ9ESG9.
PhosphoSitePlusiQ9ESG9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000023908.
CleanExiMM_MYT1.
GenevisibleiQ9ESG9. MM.

Interactioni

Subunit structurei

Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024701.

Structurei

3D structure databases

ProteinModelPortaliQ9ESG9.
SMRiQ9ESG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini101 – 350Protein kinasePROSITE-ProRule annotationAdd BLAST250

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni389 – 490Interaction with PIN1By similarityAdd BLAST102
Regioni428 – 490Interaction with CDC2-CCNB1By similarityAdd BLAST63

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi373 – 389Membrane-association motifBy similarityAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 93Pro-richAdd BLAST90

Domaini

The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region of such is however unsure (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0601. Eukaryota.
ENOG410XS1M. LUCA.
GeneTreeiENSGT00530000063230.
HOGENOMiHOG000293277.
HOVERGENiHBG053623.
InParanoidiQ9ESG9.
KOiK06633.
OMAiGTFPAFE.
OrthoDBiEOG091G0OE6.
TreeFamiTF101087.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR016235. Tyr/Thr_kinase_Cdc2_inhib.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000567. TYPK_Myt1. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ESG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMPTEGTPP PLSGTPIPVP AYFRHAEPGF SLKRPGGLSR SLPPRPPAKG
60 70 80 90 100
CIPVSRLFPP RTPGWHQPQP RRVSFLCETS EPLQSPGYDP SRPESFFQQN
110 120 130 140 150
FQRLSRLGHG SYGEVFKVRS KEDGRLYAVK RYMSPFRGPK DRTRKLAEVG
160 170 180 190 200
GHEKVGQHPH CVRLERAWEE GGILYLQTEL CGPSLQQHCE AWGASLPEAQ
210 220 230 240 250
VWGYLRDILL ALDHLHSQGL VHLDVKPANI FLGPRGRCKL GDFGLLVELG
260 270 280 290 300
SAGAGEAQEG DPRYMAPELL QGSYGTAADV FSLGLTILEV ACNMELPHGG
310 320 330 340 350
EGWQQLRQGY LPPEFTAGLS SELRSVLAMM LEPDPQLRAT AEALLALPML
360 370 380 390 400
RQPRPWNVLW YMAAEALSRG WALWQALVTL LCWLWHGLVH PASWLQPPGP
410 420 430 440 450
PATPPGSPPC SPLLDSTLSS SWDNDSIGPS LSPETVLSRI TRRTSTPRGR
460 470 480 490
YIPRDALDLT DVDSEPPRGP CPTFEPRNLL SLFEDSLDPA
Length:490
Mass (Da):54,053
Last modified:July 27, 2011 - v3
Checksum:i1308CD1F9D0293B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti252A → T in AAG09210 (Ref. 1) Curated1
Sequence conflicti252A → T in AAH25061 (PubMed:15489334).Curated1
Sequence conflicti405P → L in AAG09210 (Ref. 1) Curated1
Sequence conflicti414 – 415LD → MN in AAG09210 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175892 mRNA. Translation: AAG09210.1.
AK158550 mRNA. Translation: BAE34554.1.
AK165164 mRNA. Translation: BAE38056.1.
BC025061 mRNA. Translation: AAH25061.1.
CCDSiCCDS28460.1.
RefSeqiNP_075545.2. NM_023058.3.
UniGeneiMm.182193.

Genome annotation databases

EnsembliENSMUST00000024701; ENSMUSP00000024701; ENSMUSG00000023908.
GeneIDi268930.
KEGGimmu:268930.
UCSCiuc008asz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF175892 mRNA. Translation: AAG09210.1.
AK158550 mRNA. Translation: BAE34554.1.
AK165164 mRNA. Translation: BAE38056.1.
BC025061 mRNA. Translation: AAH25061.1.
CCDSiCCDS28460.1.
RefSeqiNP_075545.2. NM_023058.3.
UniGeneiMm.182193.

3D structure databases

ProteinModelPortaliQ9ESG9.
SMRiQ9ESG9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000024701.

PTM databases

iPTMnetiQ9ESG9.
PhosphoSitePlusiQ9ESG9.

Proteomic databases

EPDiQ9ESG9.
PaxDbiQ9ESG9.
PeptideAtlasiQ9ESG9.
PRIDEiQ9ESG9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024701; ENSMUSP00000024701; ENSMUSG00000023908.
GeneIDi268930.
KEGGimmu:268930.
UCSCiuc008asz.1. mouse.

Organism-specific databases

CTDi9088.
MGIiMGI:2137630. Pkmyt1.

Phylogenomic databases

eggNOGiKOG0601. Eukaryota.
ENOG410XS1M. LUCA.
GeneTreeiENSGT00530000063230.
HOGENOMiHOG000293277.
HOVERGENiHBG053623.
InParanoidiQ9ESG9.
KOiK06633.
OMAiGTFPAFE.
OrthoDBiEOG091G0OE6.
TreeFamiTF101087.

Enzyme and pathway databases

ReactomeiR-MMU-156711. Polo-like kinase mediated events.
R-MMU-69273. Cyclin A/B1 associated events during G2/M transition.
R-MMU-69478. G2/M DNA replication checkpoint.

Miscellaneous databases

ChiTaRSiPkmyt1. mouse.
PROiQ9ESG9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023908.
CleanExiMM_MYT1.
GenevisibleiQ9ESG9. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR016235. Tyr/Thr_kinase_Cdc2_inhib.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000567. TYPK_Myt1. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMYT1_MOUSE
AccessioniPrimary (citable) accession number: Q9ESG9
Secondary accession number(s): Q3TNN2, Q8R3L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.