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Protein

Heparan sulfate glucosamine 3-O-sulfotransferase 1

Gene

Hs3st1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site (By similarity).By similarity

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei151 – 1511PAPSBy similarity
Binding sitei159 – 1591PAPSBy similarity
Binding sitei259 – 2591PAPSBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi68 – 725PAPSBy similarity
Nucleotide bindingi274 – 2785PAPSBy similarity

GO - Molecular functioni

  • [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Heparan sulfate glucosamine 3-O-sulfotransferase 1 (EC:2.8.2.23)
Alternative name(s):
Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1
Short name:
Heparan sulfate 3-O-sulfotransferase 1
Gene namesi
Name:Hs3st1
Synonyms:3ost, 3ost1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi71084. Hs3st1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Chaini21 – 311291Heparan sulfate glucosamine 3-O-sulfotransferase 1PRO_0000033453Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi52 – 521N-linked (GlcNAc...)Sequence analysis
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence analysis
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence analysis
Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence analysis
Disulfide bondi260 ↔ 269By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9ESG5.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014077.

Structurei

3D structure databases

ProteinModelPortaliQ9ESG5.
SMRiQ9ESG5. Positions 54-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ9ESG5.
KOiK01024.
PhylomeDBiQ9ESG5.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ESG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLLLGAVL LVAQPQLVPS HPAAPGPGLK QQGLLRKVII LPEDTGEGAA
60 70 80 90 100
TNGSTQQLPQ TIIIGVRKGG TRALLEMLSL HPDVAAAENE VHFFDWEEHY
110 120 130 140 150
SQGLGWYLTQ MPFSSPHQLT VEKTPAYFTS PKVPERIHSM NPTIRLLLIL
160 170 180 190 200
RDPSERVLSD YTQVLYNHLQ KHKPYPPIED LLMRDGRLNV DYKALNRSLY
210 220 230 240 250
HAHMLNWLRF FPLGHIHIVD GDRFIRDPFP EIQKVERFLK LSPQINASNF
260 270 280 290 300
YFNKTKGFYC LRDSGKDRCL HESKGRAHPQ VDPKLLDKLH EYFREPNKKF
310
FKLVGRTFDW H
Length:311
Mass (Da):35,810
Last modified:March 1, 2001 - v1
Checksum:iACEE78B365EBCB75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177430 mRNA. Translation: AAG09283.1.
RefSeqiNP_445843.1. NM_053391.1.
UniGeneiRn.17364.

Genome annotation databases

GeneIDi84406.
KEGGirno:84406.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177430 mRNA. Translation: AAG09283.1.
RefSeqiNP_445843.1. NM_053391.1.
UniGeneiRn.17364.

3D structure databases

ProteinModelPortaliQ9ESG5.
SMRiQ9ESG5. Positions 54-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014077.

Proteomic databases

PaxDbiQ9ESG5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84406.
KEGGirno:84406.

Organism-specific databases

CTDi9957.
RGDi71084. Hs3st1.

Phylogenomic databases

eggNOGiKOG3704. Eukaryota.
ENOG410XS59. LUCA.
HOGENOMiHOG000036663.
HOVERGENiHBG053377.
InParanoidiQ9ESG5.
KOiK01024.
PhylomeDBiQ9ESG5.

Miscellaneous databases

PROiQ9ESG5.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Expression of N-deacetylase/sulfotransferase and 3-O-sulfotransferase in rat alveolar type II cells."
    Li Z.-Y., Hirayoshi K., Suzuki Y.
    Am. J. Physiol. 279:L292-L301(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.

Entry informationi

Entry nameiHS3S1_RAT
AccessioniPrimary (citable) accession number: Q9ESG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.