Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Prostasin

Gene

Prss8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates (By similarity). Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Charge relay systemBy similarity
Active sitei134 – 1341Charge relay systemBy similarity
Active sitei238 – 2381Charge relay systemBy similarity

GO - Molecular functioni

  • serine-type endopeptidase activity Source: InterPro
  • sodium channel regulator activity Source: UniProtKB

GO - Biological processi

  • positive regulation of sodium ion transport Source: MGI
  • transepithelial transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.159.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostasin (EC:3.4.21.-)
Alternative name(s):
Channel-activating protease 1
Short name:
CAP1
Serine protease 8
Cleaved into the following 2 chains:
Gene namesi
Name:Prss8
Synonyms:Cap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1923810. Prss8.

Subcellular locationi

Chain Prostasin light chain :
  • Secretedextracellular space By similarity

  • Note: Found in the seminal fluid. Secreted after cleavage of its C-terminus.By similarity
Chain Prostasin heavy chain :
  • Secretedextracellular space By similarity

  • Note: Found in the seminal fluid. Secreted after cleavage of its C-terminus.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei320 – 34021HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • anchored component of plasma membrane Source: UniProtKB
  • extracellular exosome Source: MGI
  • extracellular space Source: UniProtKB
  • extrinsic component of plasma membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • membrane raft Source: UniProtKB
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Propeptidei30 – 323Activation peptideBy similarityPRO_0000028031
Chaini33 – 322290ProstasinPRO_0000240512Add
BLAST
Chaini33 – 4412Prostasin light chainPRO_0000028032Add
BLAST
Chaini45 – 322278Prostasin heavy chainPRO_0000028033Add
BLAST
Propeptidei323 – 34220By similarityPRO_0000028034Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi37 ↔ 154Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi70 ↔ 86PROSITE-ProRule annotation
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence analysis
Glycosylationi159 – 1591N-linked (GlcNAc...)Sequence analysis
Disulfide bondi168 ↔ 244PROSITE-ProRule annotation
Disulfide bondi201 ↔ 223PROSITE-ProRule annotation
Disulfide bondi234 ↔ 262PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9ESD1.
PRIDEiQ9ESD1.

Expressioni

Gene expression databases

CleanExiMM_PRSS8.

Interactioni

Subunit structurei

Heterodimer of two chains, light and heavy, held by a disulfide bond.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032988.

Structurei

3D structure databases

ProteinModelPortaliQ9ESD1.
SMRiQ9ESD1. Positions 45-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 286242Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9ESD1.
PhylomeDBiQ9ESD1.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ESD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPRVGLGLG QLEAVTILLL LGLLQSGIRA DGTEASCGAV IQPRITGGGS
60 70 80 90 100
AKPGQWPWQV SITYDGNHVC GGSLVSNKWV VSAAHCFPRE HSREAYEVKL
110 120 130 140 150
GAHQLDSYSN DTVVHTVAQI ITHSSYREEG SQGDIAFIRL SSPVTFSRYI
160 170 180 190 200
RPICLPAANA SFPNGLHCTV TGWGHVAPSV SLQTPRPLQQ LEVPLISRET
210 220 230 240 250
CSCLYNINAV PEEPHTIQQD MLCAGYVKGG KDACQGDSGG PLSCPMEGIW
260 270 280 290 300
YLAGIVSWGD ACGAPNRPGV YTLTSTYASW IHHHVAELQP RVVPQTQESQ
310 320 330 340
PDGHLCNHHP VFSSAAAPKL LRPVLFLPLG LTLGLLSLWL EH
Length:342
Mass (Da):36,729
Last modified:April 27, 2001 - v2
Checksum:i0620DE88ED187D0F
GO

Sequence cautioni

The sequence AAG17054.1 differs from that shown. Reason: Frameshift at position 339. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF188613 mRNA. Translation: AAG17054.1. Frameshift.
UniGeneiMm.5875.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF188613 mRNA. Translation: AAG17054.1. Frameshift.
UniGeneiMm.5875.

3D structure databases

ProteinModelPortaliQ9ESD1.
SMRiQ9ESD1. Positions 45-289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032988.

Protein family/group databases

MEROPSiS01.159.

Proteomic databases

PaxDbiQ9ESD1.
PRIDEiQ9ESD1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1923810. Prss8.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9ESD1.
PhylomeDBiQ9ESD1.

Miscellaneous databases

PROiQ9ESD1.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PRSS8.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Activation of the amiloride-sensitive epithelial sodium channel by the serine protease mCAP1 expressed in a mouse cortical collecting duct cell line."
    Vuagniaux G., Vallet V., Jaeger N.F., Pfister C., Bens M., Farman N., Courtois-Coutry N., Vandewalle A., Rossier B.C., Hummler E.
    J. Am. Soc. Nephrol. 11:828-834(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.

Entry informationi

Entry nameiPRSS8_MOUSE
AccessioniPrimary (citable) accession number: Q9ESD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: March 16, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.