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Protein

Reticulon-3

Gene

Rtn3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress (By similarity). Induces the formation of endoplasmic reticulum tubules (PubMed:24262037).By similarity1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, ER-Golgi transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-8849932. SALM protein interactions at the synapse.

Names & Taxonomyi

Protein namesi
Recommended name:
Reticulon-3
Gene namesi
Name:Rtn3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1339970. Rtn3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 795794CytoplasmicSequence analysisAdd
BLAST
Intramembranei796 – 81924HelicalSequence analysisAdd
BLAST
Topological domaini820 – 87657CytoplasmicSequence analysisAdd
BLAST
Intramembranei877 – 89923HelicalSequence analysisAdd
BLAST
Topological domaini900 – 9034CytoplasmicSequence analysis
Intramembranei904 – 92623HelicalSequence analysisAdd
BLAST
Topological domaini927 – 96438CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 964963Reticulon-3PRO_0000168164Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei31 – 311PhosphoserineBy similarity
Modified residuei217 – 2171PhosphoserineCombined sources
Modified residuei225 – 2251PhosphoserineCombined sources
Modified residuei230 – 2301PhosphoserineCombined sources
Modified residuei233 – 2331PhosphoserineCombined sources
Modified residuei270 – 2701PhosphoserineBy similarity
Modified residuei303 – 3031PhosphoserineBy similarity
Modified residuei429 – 4291PhosphoserineBy similarity
Modified residuei529 – 5291PhosphoserineCombined sources
Modified residuei593 – 5931PhosphothreonineBy similarity
Modified residuei596 – 5961PhosphoserineCombined sources
Modified residuei597 – 5971PhosphoserineCombined sources
Modified residuei673 – 6731PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9ES97.
PaxDbiQ9ES97.
PeptideAtlasiQ9ES97.
PRIDEiQ9ES97.
TopDownProteomicsiQ9ES97-1. [Q9ES97-1]
Q9ES97-3. [Q9ES97-3]
Q9ES97-4. [Q9ES97-4]
Q9ES97-5. [Q9ES97-5]

PTM databases

iPTMnetiQ9ES97.
PhosphoSiteiQ9ES97.
SwissPalmiQ9ES97.

Expressioni

Tissue specificityi

Isoform 1, isoform 3, isoform 4 and isoform 5 are expressed in spinal cord. Isoform 1 is present in brain, where it is expressed in the neurons of cerebral cortex, hippocampus, hypothalamus and cerebellum (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000024758.
CleanExiMM_RTN3.
GenevisibleiQ9ES97. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with RTN4. Isoform 3 interacts with BACE1, BACE2, BCL2 and FADD (By similarity). Interacts with ATL1 and ATL2. Isoform 3 interacts with TMEM33 (PubMed:25612671).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATL1Q8WXF73EBI-1487798,EBI-2410266From a different organism.
ATL2Q8NHH92EBI-1487798,EBI-2410430From a different organism.

Protein-protein interaction databases

BioGridi203034. 1 interaction.
IntActiQ9ES97. 7 interactions.
MINTiMINT-1743916.
STRINGi10090.ENSMUSP00000065810.

Structurei

3D structure databases

ProteinModelPortaliQ9ES97.
SMRiQ9ES97. Positions 826-891.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini776 – 964189ReticulonPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni919 – 96446Interaction with FADDBy similarityAdd
BLAST
Regioni932 – 9343Interaction with BACE1By similarity

Sequence similaritiesi

Contains 1 reticulon domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1792. Eukaryota.
ENOG410XPKH. LUCA.
GeneTreeiENSGT00390000009934.
HOVERGENiHBG093922.
InParanoidiQ9ES97.
OMAiQFSHTTA.
OrthoDBiEOG091G0TIO.
PhylomeDBiQ9ES97.
TreeFamiTF105431.

Family and domain databases

InterProiIPR003388. Reticulon.
[Graphical view]
PfamiPF02453. Reticulon. 1 hit.
[Graphical view]
PROSITEiPS50845. RETICULON. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ES97-1) [UniParc]FASTAAdd to basket
Also known as: A1, A4b, B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAESSAATQS PSVSSSSSGA EPSALGGGGG SPGACPALGA KSCGSSCADS
60 70 80 90 100
FVSSSSSQPV SIFSTSQAGL SSLCSDEPPS KSMTSSFLSS SEIHNPDPTT
110 120 130 140 150
PLGEKSETLG SQFVLAKGKD PLVLLDKKKL DSPQGTNKDR VDAPVSLATG
160 170 180 190 200
IPCSHPSIPD SFPEQPAFLS KEIGPAEEWV VKDQEPKNPN KVPDGEDRSA
210 220 230 240 250
LDFGQSKAEH ICTYSLSPSE LPVASVEKDS PESPFEVIID KATFDREFKD
260 270 280 290 300
LYKENPNDLG GWAAHGDRES PADLLEMNDK LFPLRNKEAG RYPSSVLLGR
310 320 330 340 350
QFSHTTAALE EVSRCVNDMH NFTNEILTWD LDPQAKQQAN KTSCTTESTG
360 370 380 390 400
LDRSELRSEI PVINLKTNPQ QKMPVCSFNG STPITKSTGD WTEAFTEGKP
410 420 430 440 450
VRDYLSSTKE AGGNGVPGSS QLHSELPGSM PEKWVSGSGA ATVEVTLPNL
460 470 480 490 500
RGAWPNSVMG EVTEVDSSGE SDDTVIEDIT EKPDSLPSAA AKTSEREIKE
510 520 530 540 550
TPSRETVRSE MCENSEQPQA QPETPTQKSL EGEVASQVPN TLNEVTPEKL
560 570 580 590 600
DMTNNPKVCS AAPPSVLNET GFSLTVPASA KLESLLGKYV EDTDGSSPED
610 620 630 640 650
LMAVLTGAEE KGIVDKEEGD VLEAVLEKIA DFKNTLPVEL LHESELSGSE
660 670 680 690 700
TKNIKSKYSE DSRETTGGAP TMSPDLEQEQ LTIRAIKELG ERQAEKVQDE
710 720 730 740 750
GISSGGKLKQ TFAPQSGPQS SSDILEHTDV KTGSDLGIPK NPTIIKNTRI
760 770 780 790 800
DSISSLTKTE MVNKNVLARL LSDFPVHDLI FWRDVKKTGF VFGTTLIMLL
810 820 830 840 850
SLAAFSVISV VSYLILALLS VTISFRVYKS VIQAVQKSEE GHPFKAYLDV
860 870 880 890 900
DITLSSEAFH NYMNAAMVHV NKALKLIIRL FLVEDLVDSL KLAVFMWLMT
910 920 930 940 950
YVGAVFNGIT LLILAELLVF SVPIVYEKYK TQIDHYVGIA RDQTKSIVEK
960
IQAKLPGIAK KKAE
Length:964
Mass (Da):103,879
Last modified:March 20, 2007 - v2
Checksum:i5170809A696F8F7A
GO
Isoform 2 (identifier: Q9ES97-2) [UniParc]FASTAAdd to basket
Also known as: A2, A3b

The sequence of this isoform differs from the canonical sequence as follows:
     49-67: Missing.

Show »
Length:945
Mass (Da):101,920
Checksum:i28DD47E72CBEC824
GO
Isoform 3 (identifier: Q9ES97-3) [UniParc]FASTAAdd to basket
Also known as: B1, A1, Rtn3c

The sequence of this isoform differs from the canonical sequence as follows:
     49-775: Missing.

Show »
Length:237
Mass (Da):25,428
Checksum:iEB60A0A7AC45F0DE
GO
Isoform 4 (identifier: Q9ES97-4) [UniParc]FASTAAdd to basket
Also known as: B2, A2

The sequence of this isoform differs from the canonical sequence as follows:
     68-775: Missing.

Show »
Length:256
Mass (Da):27,387
Checksum:i5EE394BAA15E38B0
GO
Isoform 5 (identifier: Q9ES97-5) [UniParc]FASTAAdd to basket
Also known as: A4a

The sequence of this isoform differs from the canonical sequence as follows:
     68-388: Missing.

Show »
Length:643
Mass (Da):68,770
Checksum:i15E3335B280533F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171S → P in AAR98631 (PubMed:15350194).Curated
Sequence conflicti215 – 2151S → P in AAR98632 (PubMed:15350194).Curated
Sequence conflicti355 – 3551E → G in AAR98632 (PubMed:15350194).Curated
Sequence conflicti899 – 8991M → I in BAC36028 (PubMed:16141072).Curated
Sequence conflicti964 – 9641E → K in BAE28664 (PubMed:16141072).Curated
Sequence conflicti964 – 9641E → K in BAE28699 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei49 – 775727Missing in isoform 3. 6 PublicationsVSP_023762Add
BLAST
Alternative sequencei49 – 6719Missing in isoform 2. 1 PublicationVSP_023763Add
BLAST
Alternative sequencei68 – 775708Missing in isoform 4. CuratedVSP_023764Add
BLAST
Alternative sequencei68 – 388321Missing in isoform 5. 1 PublicationVSP_023765Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046114 mRNA. Translation: BAB62070.1.
BK001796 mRNA. Translation: DAA01968.1.
AY164700 mRNA. Translation: AAP47278.1.
AY507126 mRNA. Translation: AAR98631.1.
AY507127 mRNA. Translation: AAR98632.1.
AY427822 mRNA. Translation: AAR08193.1.
AY750849 mRNA. Translation: AAU81931.1.
AF195940 mRNA. Translation: AAG31360.1.
AK032109 mRNA. Translation: BAC27708.1.
AK036892 mRNA. Translation: BAC29625.1.
AK049845 mRNA. Translation: BAC33952.1.
AK075883 mRNA. Translation: BAC36028.1.
AK088670 mRNA. Translation: BAC40493.1.
AK146505 mRNA. Translation: BAE27220.1.
AK148792 mRNA. Translation: BAE28664.1.
AK148947 mRNA. Translation: BAE28699.1.
AK165683 mRNA. Translation: BAE38336.1.
BC014697 mRNA. Translation: AAH14697.1.
BC036717 mRNA. Translation: AAH36717.1.
BC079882 mRNA. Translation: AAH79882.1.
CCDSiCCDS29525.1. [Q9ES97-2]
CCDS29526.1. [Q9ES97-3]
CCDS37906.1. [Q9ES97-1]
CCDS70927.1. [Q9ES97-4]
RefSeqiNP_001003933.1. NM_001003933.2. [Q9ES97-2]
NP_001003934.1. NM_001003934.2. [Q9ES97-1]
NP_001258415.1. NM_001271486.1. [Q9ES97-5]
NP_001258416.1. NM_001271487.1. [Q9ES97-4]
NP_444306.1. NM_053076.3. [Q9ES97-3]
UniGeneiMm.246990.

Genome annotation databases

EnsembliENSMUST00000025667; ENSMUSP00000025667; ENSMUSG00000024758. [Q9ES97-3]
ENSMUST00000065304; ENSMUSP00000065810; ENSMUSG00000024758. [Q9ES97-1]
ENSMUST00000088169; ENSMUSP00000085494; ENSMUSG00000024758. [Q9ES97-4]
ENSMUST00000088171; ENSMUSP00000085496; ENSMUSG00000024758. [Q9ES97-2]
GeneIDi20168.
KEGGimmu:20168.
UCSCiuc008glb.2. mouse. [Q9ES97-1]
uc008gld.2. mouse. [Q9ES97-2]
uc008gle.2. mouse. [Q9ES97-3]
uc012bhv.2. mouse. [Q9ES97-5]
uc033hiw.1. mouse. [Q9ES97-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB046114 mRNA. Translation: BAB62070.1.
BK001796 mRNA. Translation: DAA01968.1.
AY164700 mRNA. Translation: AAP47278.1.
AY507126 mRNA. Translation: AAR98631.1.
AY507127 mRNA. Translation: AAR98632.1.
AY427822 mRNA. Translation: AAR08193.1.
AY750849 mRNA. Translation: AAU81931.1.
AF195940 mRNA. Translation: AAG31360.1.
AK032109 mRNA. Translation: BAC27708.1.
AK036892 mRNA. Translation: BAC29625.1.
AK049845 mRNA. Translation: BAC33952.1.
AK075883 mRNA. Translation: BAC36028.1.
AK088670 mRNA. Translation: BAC40493.1.
AK146505 mRNA. Translation: BAE27220.1.
AK148792 mRNA. Translation: BAE28664.1.
AK148947 mRNA. Translation: BAE28699.1.
AK165683 mRNA. Translation: BAE38336.1.
BC014697 mRNA. Translation: AAH14697.1.
BC036717 mRNA. Translation: AAH36717.1.
BC079882 mRNA. Translation: AAH79882.1.
CCDSiCCDS29525.1. [Q9ES97-2]
CCDS29526.1. [Q9ES97-3]
CCDS37906.1. [Q9ES97-1]
CCDS70927.1. [Q9ES97-4]
RefSeqiNP_001003933.1. NM_001003933.2. [Q9ES97-2]
NP_001003934.1. NM_001003934.2. [Q9ES97-1]
NP_001258415.1. NM_001271486.1. [Q9ES97-5]
NP_001258416.1. NM_001271487.1. [Q9ES97-4]
NP_444306.1. NM_053076.3. [Q9ES97-3]
UniGeneiMm.246990.

3D structure databases

ProteinModelPortaliQ9ES97.
SMRiQ9ES97. Positions 826-891.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203034. 1 interaction.
IntActiQ9ES97. 7 interactions.
MINTiMINT-1743916.
STRINGi10090.ENSMUSP00000065810.

PTM databases

iPTMnetiQ9ES97.
PhosphoSiteiQ9ES97.
SwissPalmiQ9ES97.

Proteomic databases

MaxQBiQ9ES97.
PaxDbiQ9ES97.
PeptideAtlasiQ9ES97.
PRIDEiQ9ES97.
TopDownProteomicsiQ9ES97-1. [Q9ES97-1]
Q9ES97-3. [Q9ES97-3]
Q9ES97-4. [Q9ES97-4]
Q9ES97-5. [Q9ES97-5]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025667; ENSMUSP00000025667; ENSMUSG00000024758. [Q9ES97-3]
ENSMUST00000065304; ENSMUSP00000065810; ENSMUSG00000024758. [Q9ES97-1]
ENSMUST00000088169; ENSMUSP00000085494; ENSMUSG00000024758. [Q9ES97-4]
ENSMUST00000088171; ENSMUSP00000085496; ENSMUSG00000024758. [Q9ES97-2]
GeneIDi20168.
KEGGimmu:20168.
UCSCiuc008glb.2. mouse. [Q9ES97-1]
uc008gld.2. mouse. [Q9ES97-2]
uc008gle.2. mouse. [Q9ES97-3]
uc012bhv.2. mouse. [Q9ES97-5]
uc033hiw.1. mouse. [Q9ES97-4]

Organism-specific databases

CTDi10313.
MGIiMGI:1339970. Rtn3.

Phylogenomic databases

eggNOGiKOG1792. Eukaryota.
ENOG410XPKH. LUCA.
GeneTreeiENSGT00390000009934.
HOVERGENiHBG093922.
InParanoidiQ9ES97.
OMAiQFSHTTA.
OrthoDBiEOG091G0TIO.
PhylomeDBiQ9ES97.
TreeFamiTF105431.

Enzyme and pathway databases

ReactomeiR-MMU-8849932. SALM protein interactions at the synapse.

Miscellaneous databases

ChiTaRSiRtn3. mouse.
PROiQ9ES97.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024758.
CleanExiMM_RTN3.
GenevisibleiQ9ES97. MM.

Family and domain databases

InterProiIPR003388. Reticulon.
[Graphical view]
PfamiPF02453. Reticulon. 1 hit.
[Graphical view]
PROSITEiPS50845. RETICULON. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRTN3_MOUSE
AccessioniPrimary (citable) accession number: Q9ES97
Secondary accession number(s): Q3UF62
, Q544J1, Q68FE4, Q6IM69, Q6R8K6, Q6R8K7, Q6T929, Q8C6D5, Q8CCU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 20, 2007
Last modified: September 7, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.