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Protein

Exostosin-like 2

Gene

Extl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan = UDP + alpha-N-acetyl-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan.1 Publication

Cofactori

Mn2+1 Publication

Kineticsi

  1. KM=320 µM for UDP-GalNAc1 Publication
  2. KM=560 µM for UDP-GlcNAc1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei101Substrate1 Publication1
    Metal bindingi153Manganese; catalytic1 Publication1
    Binding sitei181Substrate1 Publication1
    Active sitei2461 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Protein family/group databases

    CAZyiGT64. Glycosyltransferase Family 64.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exostosin-like 2 (EC:2.4.1.2231 Publication)
    Alternative name(s):
    Alpha-1,4-N-acetylhexosaminyltransferase EXTL2
    Alpha-GalNAcT EXTL2
    EXT-related protein 2
    Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
    Gene namesi
    Name:Extl2
    Synonyms:Extr2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 3

    Organism-specific databases

    MGIiMGI:1889574. Extl2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
    Transmembranei22 – 42Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini43 – 330LumenalSequence analysisAdd BLAST288

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi243N → A: Reduced activity. 1 Publication1
    Mutagenesisi246D → A: Abolishes enzyme activity. 1 Publication1
    Mutagenesisi293R → A: Reduced activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001496561 – 330Exostosin-like 2Add BLAST330

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi75N-linked (GlcNAc...)Sequence analysis1
    Disulfide bondi244 ↔ 2961 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ9ES89.
    PaxDbiQ9ES89.
    PRIDEiQ9ES89.

    PTM databases

    PhosphoSitePlusiQ9ES89.

    Expressioni

    Gene expression databases

    BgeeiENSMUSG00000027963.
    CleanExiMM_EXTL2.
    ExpressionAtlasiQ9ES89. baseline and differential.
    GenevisibleiQ9ES89. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000029575.

    Structurei

    Secondary structure

    1330
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi67 – 75Combined sources9
    Helixi77 – 87Combined sources11
    Beta strandi93 – 100Combined sources8
    Helixi109 – 114Combined sources6
    Beta strandi122 – 126Combined sources5
    Helixi132 – 136Combined sources5
    Beta strandi144 – 150Combined sources7
    Beta strandi154 – 156Combined sources3
    Helixi158 – 168Combined sources11
    Beta strandi174 – 178Combined sources5
    Beta strandi180 – 187Combined sources8
    Beta strandi190 – 194Combined sources5
    Beta strandi202 – 206Combined sources5
    Beta strandi209 – 212Combined sources4
    Beta strandi216 – 220Combined sources5
    Helixi222 – 228Combined sources7
    Helixi232 – 241Combined sources10
    Helixi245 – 257Combined sources13
    Beta strandi261 – 265Combined sources5
    Beta strandi268 – 272Combined sources5
    Beta strandi276 – 279Combined sources4
    Helixi283 – 285Combined sources3
    Helixi288 – 304Combined sources17
    Beta strandi314 – 318Combined sources5
    Turni323 – 326Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1OMXX-ray2.40A/B38-330[»]
    1OMZX-ray2.10A/B38-330[»]
    1ON6X-ray2.30A/B38-330[»]
    1ON8X-ray2.70A/B38-330[»]
    DisProtiDP00397.
    ProteinModelPortaliQ9ES89.
    SMRiQ9ES89.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9ES89.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni130 – 135Substrate binding1 Publication6
    Regioni151 – 153Substrate binding1 Publication3
    Regioni242 – 246Substrate binding1 Publication5
    Regioni280 – 293Substrate binding1 PublicationAdd BLAST14

    Sequence similaritiesi

    Belongs to the glycosyltransferase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1022. Eukaryota.
    ENOG410XQYV. LUCA.
    GeneTreeiENSGT00550000074496.
    HOGENOMiHOG000054215.
    HOVERGENiHBG051525.
    InParanoidiQ9ES89.
    KOiK02369.
    OMAiGCHICKL.
    OrthoDBiEOG091G0A1H.
    PhylomeDBiQ9ES89.
    TreeFamiTF314231.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR015338. EXT_C.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF09258. Glyco_transf_64. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9ES89-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MMRGCHICKL PGRVMGIRVL RFSLVVILVL LLVAGALTNL LPNIKEDKML
    60 70 80 90 100
    TLRREIKSPS KSALDSFTLI MQTYNRTDLL LRLLNHYQAV PSLHKVIVVW
    110 120 130 140 150
    NNVGEKGPEE LWNSLGPHPI PVIFKPQTAN KMRNRLQVFP EVETNAVLMV
    160 170 180 190 200
    DDDTLISAQD LVFAFSIWQQ FPDQIIGFVP RKHVSTSSGI YSYGGFELQT
    210 220 230 240 250
    PGPGNGDQYS MVLIGASFFN SKYLELFQKQ PAAVHALIDE TQNCDDIAMN
    260 270 280 290 300
    FLVTRHTGKP SGIFVKPINM VNLEKETNGY SGMWHRAEHF LQRSYCINKL
    310 320 330
    VNIYDGMPLK YSNIMISQFG FPYANHKSKM
    Length:330
    Mass (Da):37,391
    Last modified:March 1, 2001 - v1
    Checksum:i51F88BE5D3E5EADF
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti191Y → D in BAB31683 (PubMed:16141072).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF200973 mRNA. Translation: AAG17542.1.
    AK019370 mRNA. Translation: BAB31683.2.
    BC031438 mRNA. Translation: AAH31438.1.
    BC094444 mRNA. Translation: AAH94444.1.
    CCDSiCCDS17784.1.
    RefSeqiNP_001156986.1. NM_001163514.1.
    NP_067363.3. NM_021388.4.
    UniGeneiMm.41739.

    Genome annotation databases

    EnsembliENSMUST00000029575; ENSMUSP00000029575; ENSMUSG00000027963.
    ENSMUST00000106502; ENSMUSP00000102111; ENSMUSG00000027963.
    GeneIDi58193.
    KEGGimmu:58193.
    UCSCiuc008rbu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF200973 mRNA. Translation: AAG17542.1.
    AK019370 mRNA. Translation: BAB31683.2.
    BC031438 mRNA. Translation: AAH31438.1.
    BC094444 mRNA. Translation: AAH94444.1.
    CCDSiCCDS17784.1.
    RefSeqiNP_001156986.1. NM_001163514.1.
    NP_067363.3. NM_021388.4.
    UniGeneiMm.41739.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1OMXX-ray2.40A/B38-330[»]
    1OMZX-ray2.10A/B38-330[»]
    1ON6X-ray2.30A/B38-330[»]
    1ON8X-ray2.70A/B38-330[»]
    DisProtiDP00397.
    ProteinModelPortaliQ9ES89.
    SMRiQ9ES89.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000029575.

    Protein family/group databases

    CAZyiGT64. Glycosyltransferase Family 64.

    PTM databases

    PhosphoSitePlusiQ9ES89.

    Proteomic databases

    MaxQBiQ9ES89.
    PaxDbiQ9ES89.
    PRIDEiQ9ES89.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000029575; ENSMUSP00000029575; ENSMUSG00000027963.
    ENSMUST00000106502; ENSMUSP00000102111; ENSMUSG00000027963.
    GeneIDi58193.
    KEGGimmu:58193.
    UCSCiuc008rbu.2. mouse.

    Organism-specific databases

    CTDi2135.
    MGIiMGI:1889574. Extl2.

    Phylogenomic databases

    eggNOGiKOG1022. Eukaryota.
    ENOG410XQYV. LUCA.
    GeneTreeiENSGT00550000074496.
    HOGENOMiHOG000054215.
    HOVERGENiHBG051525.
    InParanoidiQ9ES89.
    KOiK02369.
    OMAiGCHICKL.
    OrthoDBiEOG091G0A1H.
    PhylomeDBiQ9ES89.
    TreeFamiTF314231.

    Miscellaneous databases

    ChiTaRSiExtl2. mouse.
    EvolutionaryTraceiQ9ES89.
    PROiQ9ES89.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000027963.
    CleanExiMM_EXTL2.
    ExpressionAtlasiQ9ES89. baseline and differential.
    GenevisibleiQ9ES89. MM.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR015338. EXT_C.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF09258. Glyco_transf_64. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiEXTL2_MOUSE
    AccessioniPrimary (citable) accession number: Q9ES89
    Secondary accession number(s): Q505Q8, Q9CX90
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: March 1, 2001
    Last modified: November 2, 2016
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.