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Protein

Exostosin-like 2

Gene

Extl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan = UDP + alpha-N-acetyl-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan.1 Publication

Cofactori

Mn2+1 Publication

Kineticsi

  1. KM=320 µM for UDP-GalNAc1 Publication
  2. KM=560 µM for UDP-GlcNAc1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei101 – 1011Substrate1 Publication
    Metal bindingi153 – 1531Manganese; catalytic1 Publication
    Binding sitei181 – 1811Substrate1 Publication
    Active sitei246 – 24611 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Protein family/group databases

    CAZyiGT64. Glycosyltransferase Family 64.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exostosin-like 2 (EC:2.4.1.2231 Publication)
    Alternative name(s):
    Alpha-1,4-N-acetylhexosaminyltransferase EXTL2
    Alpha-GalNAcT EXTL2
    EXT-related protein 2
    Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
    Gene namesi
    Name:Extl2
    Synonyms:Extr2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 3

    Organism-specific databases

    MGIiMGI:1889574. Extl2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2121CytoplasmicSequence analysisAdd
    BLAST
    Transmembranei22 – 4221Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
    BLAST
    Topological domaini43 – 330288LumenalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi243 – 2431N → A: Reduced activity. 1 Publication
    Mutagenesisi246 – 2461D → A: Abolishes enzyme activity. 1 Publication
    Mutagenesisi293 – 2931R → A: Reduced activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 330330Exostosin-like 2PRO_0000149656Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence analysis
    Disulfide bondi244 ↔ 2961 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ9ES89.
    PaxDbiQ9ES89.
    PRIDEiQ9ES89.

    PTM databases

    PhosphoSiteiQ9ES89.

    Expressioni

    Gene expression databases

    BgeeiENSMUSG00000027963.
    CleanExiMM_EXTL2.
    ExpressionAtlasiQ9ES89. baseline and differential.
    GenevisibleiQ9ES89. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000029575.

    Structurei

    Secondary structure

    1
    330
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi67 – 759Combined sources
    Helixi77 – 8711Combined sources
    Beta strandi93 – 1008Combined sources
    Helixi109 – 1146Combined sources
    Beta strandi122 – 1265Combined sources
    Helixi132 – 1365Combined sources
    Beta strandi144 – 1507Combined sources
    Beta strandi154 – 1563Combined sources
    Helixi158 – 16811Combined sources
    Beta strandi174 – 1785Combined sources
    Beta strandi180 – 1878Combined sources
    Beta strandi190 – 1945Combined sources
    Beta strandi202 – 2065Combined sources
    Beta strandi209 – 2124Combined sources
    Beta strandi216 – 2205Combined sources
    Helixi222 – 2287Combined sources
    Helixi232 – 24110Combined sources
    Helixi245 – 25713Combined sources
    Beta strandi261 – 2655Combined sources
    Beta strandi268 – 2725Combined sources
    Beta strandi276 – 2794Combined sources
    Helixi283 – 2853Combined sources
    Helixi288 – 30417Combined sources
    Beta strandi314 – 3185Combined sources
    Turni323 – 3264Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OMXX-ray2.40A/B38-330[»]
    1OMZX-ray2.10A/B38-330[»]
    1ON6X-ray2.30A/B38-330[»]
    1ON8X-ray2.70A/B38-330[»]
    DisProtiDP00397.
    ProteinModelPortaliQ9ES89.
    SMRiQ9ES89. Positions 63-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9ES89.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni130 – 1356Substrate binding1 Publication
    Regioni151 – 1533Substrate binding1 Publication
    Regioni242 – 2465Substrate binding1 Publication
    Regioni280 – 29314Substrate binding1 PublicationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyltransferase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1022. Eukaryota.
    ENOG410XQYV. LUCA.
    GeneTreeiENSGT00550000074496.
    HOGENOMiHOG000054215.
    HOVERGENiHBG051525.
    InParanoidiQ9ES89.
    KOiK02369.
    OMAiGCHICKL.
    OrthoDBiEOG091G0A1H.
    PhylomeDBiQ9ES89.
    TreeFamiTF314231.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR015338. EXT_C.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF09258. Glyco_transf_64. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9ES89-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MMRGCHICKL PGRVMGIRVL RFSLVVILVL LLVAGALTNL LPNIKEDKML
    60 70 80 90 100
    TLRREIKSPS KSALDSFTLI MQTYNRTDLL LRLLNHYQAV PSLHKVIVVW
    110 120 130 140 150
    NNVGEKGPEE LWNSLGPHPI PVIFKPQTAN KMRNRLQVFP EVETNAVLMV
    160 170 180 190 200
    DDDTLISAQD LVFAFSIWQQ FPDQIIGFVP RKHVSTSSGI YSYGGFELQT
    210 220 230 240 250
    PGPGNGDQYS MVLIGASFFN SKYLELFQKQ PAAVHALIDE TQNCDDIAMN
    260 270 280 290 300
    FLVTRHTGKP SGIFVKPINM VNLEKETNGY SGMWHRAEHF LQRSYCINKL
    310 320 330
    VNIYDGMPLK YSNIMISQFG FPYANHKSKM
    Length:330
    Mass (Da):37,391
    Last modified:March 1, 2001 - v1
    Checksum:i51F88BE5D3E5EADF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti191 – 1911Y → D in BAB31683 (PubMed:16141072).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF200973 mRNA. Translation: AAG17542.1.
    AK019370 mRNA. Translation: BAB31683.2.
    BC031438 mRNA. Translation: AAH31438.1.
    BC094444 mRNA. Translation: AAH94444.1.
    CCDSiCCDS17784.1.
    RefSeqiNP_001156986.1. NM_001163514.1.
    NP_067363.3. NM_021388.4.
    UniGeneiMm.41739.

    Genome annotation databases

    EnsembliENSMUST00000029575; ENSMUSP00000029575; ENSMUSG00000027963.
    ENSMUST00000106502; ENSMUSP00000102111; ENSMUSG00000027963.
    GeneIDi58193.
    KEGGimmu:58193.
    UCSCiuc008rbu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF200973 mRNA. Translation: AAG17542.1.
    AK019370 mRNA. Translation: BAB31683.2.
    BC031438 mRNA. Translation: AAH31438.1.
    BC094444 mRNA. Translation: AAH94444.1.
    CCDSiCCDS17784.1.
    RefSeqiNP_001156986.1. NM_001163514.1.
    NP_067363.3. NM_021388.4.
    UniGeneiMm.41739.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OMXX-ray2.40A/B38-330[»]
    1OMZX-ray2.10A/B38-330[»]
    1ON6X-ray2.30A/B38-330[»]
    1ON8X-ray2.70A/B38-330[»]
    DisProtiDP00397.
    ProteinModelPortaliQ9ES89.
    SMRiQ9ES89. Positions 63-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000029575.

    Protein family/group databases

    CAZyiGT64. Glycosyltransferase Family 64.

    PTM databases

    PhosphoSiteiQ9ES89.

    Proteomic databases

    MaxQBiQ9ES89.
    PaxDbiQ9ES89.
    PRIDEiQ9ES89.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000029575; ENSMUSP00000029575; ENSMUSG00000027963.
    ENSMUST00000106502; ENSMUSP00000102111; ENSMUSG00000027963.
    GeneIDi58193.
    KEGGimmu:58193.
    UCSCiuc008rbu.2. mouse.

    Organism-specific databases

    CTDi2135.
    MGIiMGI:1889574. Extl2.

    Phylogenomic databases

    eggNOGiKOG1022. Eukaryota.
    ENOG410XQYV. LUCA.
    GeneTreeiENSGT00550000074496.
    HOGENOMiHOG000054215.
    HOVERGENiHBG051525.
    InParanoidiQ9ES89.
    KOiK02369.
    OMAiGCHICKL.
    OrthoDBiEOG091G0A1H.
    PhylomeDBiQ9ES89.
    TreeFamiTF314231.

    Miscellaneous databases

    ChiTaRSiExtl2. mouse.
    EvolutionaryTraceiQ9ES89.
    PROiQ9ES89.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000027963.
    CleanExiMM_EXTL2.
    ExpressionAtlasiQ9ES89. baseline and differential.
    GenevisibleiQ9ES89. MM.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR015338. EXT_C.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF09258. Glyco_transf_64. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiEXTL2_MOUSE
    AccessioniPrimary (citable) accession number: Q9ES89
    Secondary accession number(s): Q505Q8, Q9CX90
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: March 1, 2001
    Last modified: September 7, 2016
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.