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Protein

Prostasin

Gene

Prss8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G) (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Charge relay systemBy similarity1
Active sitei134Charge relay systemBy similarity1
Active sitei238Charge relay systemBy similarity1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: RGD
  • sodium channel regulator activity Source: RGD

GO - Biological processi

  • positive regulation of sodium ion transport Source: GO_Central
  • response to drug Source: RGD
  • response to mineralocorticoid Source: RGD
  • response to peptide hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.159.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostasin (EC:3.4.21.-)
Alternative name(s):
Channel-activating protease 1
Short name:
CAP1
Serine protease 8
Cleaved into the following 2 chains:
Gene namesi
Name:Prss8
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619973. Prss8.

Subcellular locationi

Chain Prostasin light chain :
  • Secretedextracellular space By similarity

  • Note: Found in the seminal fluid. Secreted after cleavage of its C-terminus.By similarity
Chain Prostasin heavy chain :
  • Secretedextracellular space By similarity

  • Note: Found in the seminal fluid. Secreted after cleavage of its C-terminus.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
PropeptideiPRO_000002803530 – 32Activation peptideBy similarity3
ChainiPRO_000024051333 – 322ProstasinAdd BLAST290
ChainiPRO_000002803633 – 44Prostasin light chainAdd BLAST12
ChainiPRO_000002803745 – 322Prostasin heavy chainAdd BLAST278
PropeptideiPRO_0000028038323 – 342By similarityAdd BLAST20

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 154Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi70 ↔ 86PROSITE-ProRule annotation
Glycosylationi159N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi168 ↔ 244PROSITE-ProRule annotation
Disulfide bondi201 ↔ 223PROSITE-ProRule annotation
Disulfide bondi234 ↔ 262PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9ES87.

Interactioni

Subunit structurei

Heterodimer of two chains, light and heavy, held by a disulfide bond.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026572.

Structurei

3D structure databases

ProteinModelPortaliQ9ES87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 286Peptidase S1PROSITE-ProRule annotationAdd BLAST242

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9ES87.
KOiK08664.
PhylomeDBiQ9ES87.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ES87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRVGLGLG QLEALFVLLL IGLLQSRIGA DGTEASCGAV IQPRITGGGS
60 70 80 90 100
AKPGQWPWQV SITYNGVHVC GGSLVSNQWV VSAAHCFPRE HSKEEYEVKL
110 120 130 140 150
GAHQLDSFSN DIVVHTVAQI ISHSSYREEG SQGDIALIRL SSPVTFSRYI
160 170 180 190 200
RPICLPAANA SFPNGLHCTV TGWGHVAPSV SLQTPRPLQQ LEVPLISRET
210 220 230 240 250
CSCLYNINAV PEEPHTIQQD MLCAGYVKGG KDACQGDSGG PLSCPIDGLW
260 270 280 290 300
YLAGIVSWGD ACGAPNRPGV YTLTSTYASW IHHHVAELQP RVVPQTQESQ
310 320 330 340
PDGHLCNHHP VFNLAAAQKL SRPILFLPLS LTLGLFSLWL EH
Length:342
Mass (Da):36,857
Last modified:May 1, 2007 - v3
Checksum:iF78BCAC8B1268C64
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17V → I in BAB20281 (PubMed:11373334).Curated1
Sequence conflicti292V → A in BAB20281 (PubMed:11373334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017638 mRNA. Translation: BAB20281.1.
AF202076 mRNA. Translation: AAG32641.1.
BC061800 mRNA. Translation: AAH61800.1.
RefSeqiNP_620191.1. NM_138836.1.
UniGeneiRn.107364.

Genome annotation databases

GeneIDi192107.
KEGGirno:192107.
UCSCiRGD:619973. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017638 mRNA. Translation: BAB20281.1.
AF202076 mRNA. Translation: AAG32641.1.
BC061800 mRNA. Translation: AAH61800.1.
RefSeqiNP_620191.1. NM_138836.1.
UniGeneiRn.107364.

3D structure databases

ProteinModelPortaliQ9ES87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026572.

Protein family/group databases

MEROPSiS01.159.

Proteomic databases

PaxDbiQ9ES87.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi192107.
KEGGirno:192107.
UCSCiRGD:619973. rat.

Organism-specific databases

CTDi5652.
RGDi619973. Prss8.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9ES87.
KOiK08664.
PhylomeDBiQ9ES87.

Miscellaneous databases

PROiQ9ES87.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRSS8_RAT
AccessioniPrimary (citable) accession number: Q9ES87
Secondary accession number(s): Q6GSY8, Q9ER01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2007
Last modified: September 7, 2016
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.