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Protein

Blood vessel epicardial substance

Gene

Bves

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion molecule involved in the establishment and/or maintenance of cell integrity. Involved in the formation and regulation of the tight junction (TJ) paracellular permeability barrier in epithelial cells (PubMed:16188940). Plays a role in VAMP3-mediated vesicular transport and recycling of different receptor molecules through its interaction with VAMP3 (PubMed:20057356). Plays a role in the regulation of cell shape and movement by modulating the Rho-family GTPase activity through its interaction with ARHGEF25/GEFT (PubMed:18541910). Induces primordial adhesive contact and aggregation of epithelial cells in a Ca2+-independent manner. Also involved in striated muscle regeneration and repair and in the regulation of cell spreading (PubMed:11839816). Important for the maintenance of cardiac function. Plays a regulatory function in heart rate dynamics mediated, at least in part, through cAMP-binding and, probably, by increasing cell surface expression of the potassium channel KCNK2 and enhancing current density (PubMed:26642364). Is a caveolae-associated protein important for the preservation of caveolae structural and functional integrity as well as for heart protection against ischemia injury (PubMed:24066022).By similarity7 Publications

GO - Molecular functioni

  • cAMP binding Source: MGI
  • structural molecule activity Source: UniProtKB

GO - Biological processi

  • cell migration involved in heart development Source: BHF-UCL
  • epithelial cell-cell adhesion Source: UniProtKB
  • heart development Source: UniProtKB
  • hematopoietic progenitor cell differentiation Source: MGI
  • positive regulation of locomotion Source: UniProtKB
  • positive regulation of receptor recycling Source: UniProtKB
  • regulation of cell shape Source: UniProtKB
  • regulation of endocytic recycling Source: BHF-UCL
  • regulation of GTPase activity Source: UniProtKB
  • regulation of heart rate Source: MGI
  • regulation of membrane potential Source: MGI
  • response to ischemia Source: UniProtKB
  • sinoatrial node cell development Source: MGI
  • skeletal muscle tissue development Source: UniProtKB
  • substrate adhesion-dependent cell spreading Source: UniProtKB
  • vesicle docking Source: BHF-UCL
  • vesicle-mediated transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

cAMP, cAMP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Blood vessel epicardial substanceImported
Short name:
mBVES
Alternative name(s):
Popeye domain-containing protein 1
Short name:
Popeye protein 1
Gene namesi
Name:BvesImported
Synonyms:Pop1Imported, Popdc1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1346013. Bves.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4848ExtracellularSequence analysisAdd
BLAST
Transmembranei49 – 6921HelicalSequence analysisAdd
BLAST
Topological domaini70 – 701CytoplasmicSequence analysis
Transmembranei71 – 9121HelicalSequence analysisAdd
BLAST
Topological domaini92 – 921ExtracellularSequence analysis
Transmembranei93 – 11321HelicalSequence analysisAdd
BLAST
Topological domaini114 – 358245CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • bicellular tight junction Source: UniProtKB
  • caveola Source: UniProtKB
  • cell projection membrane Source: BHF-UCL
  • integral component of membrane Source: UniProtKB
  • lateral plasma membrane Source: UniProtKB
  • membrane Source: MGI
  • plasma membrane Source: UniProtKB
  • sarcolemma Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Tight junction

Pathology & Biotechi

Disruption phenotypei

Skeletal muscle regeneration appears to be less efficient and delayed (PubMed:11839816). Knockout mice are deficient to adapt heart rate to physiological stress, this deficiency develops in older mice. They show severe sinus node dysfunction with long pauses and intercurrent periods of normal synus rhythm. The sinus node strucutre is abnormal with a loss of pacemaker tissue from the inferior part of the sinus node and a compact structure of the superior sinus node (PubMed:22354168). They have inpaired functional recovery after ischemia/reperfusion injury (PubMed:24066022).3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi200 – 2001D → A: Almost abolishes cAMP-binding. Interaction with KCNK2 is not inhibited by cAMP. 1 Publication
Mutagenesisi202 – 2021P → A: No effect on cAMP-binding. 1 Publication
Mutagenesisi203 – 2031E → A: Decreases cAMP-binding. 1 Publication
Mutagenesisi217 – 2171V → F: Decreases cAMP-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Blood vessel epicardial substancePRO_0000046792Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)Sequence analysis
Glycosylationi30 – 301N-linked (GlcNAc...)Sequence analysis
Modified residuei295 – 2951PhosphoserineCombined sources
Modified residuei318 – 3181PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9ES83.
PaxDbiQ9ES83.
PeptideAtlasiQ9ES83.
PRIDEiQ9ES83.

PTM databases

iPTMnetiQ9ES83.
PhosphoSiteiQ9ES83.

Expressioni

Tissue specificityi

Expressed in epithelial cells, skeletal muscle, heart and intestinal smooth muscle (at protein level). Expressed in fetal and adult heart and skeletal muscle.4 Publications

Developmental stagei

Expressed in the mesoderm of the cardiac crescent at E9.5. Expressed in cardiac myocytes of the sinoatrial compartment and some restricted areas of the dorsal part of the ventricle chambers at E10.5 and E12.5. Expressed in branchial arches, myotome and in a posterior domain in the limb at E10.5. Expressed in the heart, mainly in the compact layer myocardium, peridigital mesenchyme, the somites of the tail bud, the smooth muscle cells of the trachea and the developing bronchial tree, the smooth muscle cells lining the digestive tract, the dorsal root ganglia and the pancreas anlage at E13.5 (at protein level). Expressed in the sinoatrial compartment and some restricted areas in the dorsal part of the ventricle at E9.5, E10.5 and E11.5. At E12.5, expression was observed in the ventral half of the ventricle where it was limited to the subepicardial compact layer.2 Publications

Gene expression databases

BgeeiENSMUSG00000071317.
CleanExiMM_BVES.
GenevisibleiQ9ES83. MM.

Interactioni

Subunit structurei

Homodimer. Homodimerization requires the C-terminus cytoplasmic region (By similarity). Interacts (via the C-terminus cytoplasmic tail) with TJP1. Interacts (via the C-terminus cytoplasmic tail) with ARHGEF25/GEFT (via the DH domain). Interacts (via the C-terminus cytoplasmic tail) with VAMP3. Interacts with KCNK2; the interaction enhances KCNK2 surface expression and is inhibited by cAMP (PubMed:22354168, PubMed:26642364). Interacts with CAV3 (PubMed:24066022).By similarity5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Vamp3P630253EBI-7705661,EBI-7705696From a different organism.

Protein-protein interaction databases

DIPiDIP-46118N.
IntActiQ9ES83. 2 interactions.
MINTiMINT-7712901.
STRINGi10090.ENSMUSP00000093382.

Structurei

3D structure databases

ProteinModelPortaliQ9ES83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 11523Required for interaction with CAV31 PublicationAdd
BLAST
Regioni136 – 18651Required for interaction with KCNK2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the popeye family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IGHK. Eukaryota.
ENOG410ZTIT. LUCA.
GeneTreeiENSGT00390000002563.
HOGENOMiHOG000236292.
HOVERGENiHBG053638.
InParanoidiQ9ES83.
OMAiTIGCTLY.
OrthoDBiEOG091G0B7Q.
PhylomeDBiQ9ES83.
TreeFamiTF326644.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR006916. Popeye_prot.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR12101. PTHR12101. 1 hit.
PfamiPF04831. Popeye. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ES83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSTESIPLA QSTVAGFTSE LESLTPVPSN ETTCENWREI HHLVFHVANV
60 70 80 90 100
CFAVGLLIPT TLHLHMILLR VMLSLGCTLY VVWATLYRCA LDVMIWNSVF
110 120 130 140 150
LGINILHLSY LLYKKRPVKI EKELGGVYHR LFEPLRVPPD LFRRLTGQFC
160 170 180 190 200
MIQTLKRGQV YATEDKTSVD DRLSILLKGR MKVSYRGHFL HNIYPCAFID
210 220 230 240 250
SPEFRSTQMH KGEKFQVTIV ADDNCRFLCW SRERLTYFLE SEPFLYEIFR
260 270 280 290 300
YLIGKDITNK LYSLNDPTLN DKKVKKLEPQ MSLCTQISML EMRNSITSSS
310 320 330 340 350
DGEDGLHHFL RGSSSTASLP MSSPQQRASA KMKPIEEGVE DDDEVFVSPD

ALKVHQLP
Length:358
Mass (Da):41,016
Last modified:March 1, 2001 - v1
Checksum:iB92F1942F1713027
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF204174 mRNA. Translation: AAG23407.1.
AF124510 mRNA. No translation available.
BC132018 mRNA. Translation: AAI32019.1.
BC132044 mRNA. Translation: AAI32045.1.
AK046765 mRNA. Translation: BAC32859.1.
CCDSiCCDS23828.1.
RefSeqiNP_077247.1. NM_024285.2.
XP_006512799.1. XM_006512736.2.
XP_006512800.1. XM_006512737.1.
UniGeneiMm.332793.

Genome annotation databases

EnsembliENSMUST00000095715; ENSMUSP00000093382; ENSMUSG00000071317.
GeneIDi23828.
KEGGimmu:23828.
UCSCiuc007ezz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF204174 mRNA. Translation: AAG23407.1.
AF124510 mRNA. No translation available.
BC132018 mRNA. Translation: AAI32019.1.
BC132044 mRNA. Translation: AAI32045.1.
AK046765 mRNA. Translation: BAC32859.1.
CCDSiCCDS23828.1.
RefSeqiNP_077247.1. NM_024285.2.
XP_006512799.1. XM_006512736.2.
XP_006512800.1. XM_006512737.1.
UniGeneiMm.332793.

3D structure databases

ProteinModelPortaliQ9ES83.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46118N.
IntActiQ9ES83. 2 interactions.
MINTiMINT-7712901.
STRINGi10090.ENSMUSP00000093382.

PTM databases

iPTMnetiQ9ES83.
PhosphoSiteiQ9ES83.

Proteomic databases

MaxQBiQ9ES83.
PaxDbiQ9ES83.
PeptideAtlasiQ9ES83.
PRIDEiQ9ES83.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095715; ENSMUSP00000093382; ENSMUSG00000071317.
GeneIDi23828.
KEGGimmu:23828.
UCSCiuc007ezz.1. mouse.

Organism-specific databases

CTDi11149.
MGIiMGI:1346013. Bves.

Phylogenomic databases

eggNOGiENOG410IGHK. Eukaryota.
ENOG410ZTIT. LUCA.
GeneTreeiENSGT00390000002563.
HOGENOMiHOG000236292.
HOVERGENiHBG053638.
InParanoidiQ9ES83.
OMAiTIGCTLY.
OrthoDBiEOG091G0B7Q.
PhylomeDBiQ9ES83.
TreeFamiTF326644.

Miscellaneous databases

PROiQ9ES83.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071317.
CleanExiMM_BVES.
GenevisibleiQ9ES83. MM.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR006916. Popeye_prot.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PANTHERiPTHR12101. PTHR12101. 1 hit.
PfamiPF04831. Popeye. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPOPD1_MOUSE
AccessioniPrimary (citable) accession number: Q9ES83
Secondary accession number(s): A2RS91, Q8C8L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.