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Protein

Serine/threonine-protein kinase Nek7

Gene

Nek7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Enzyme regulationi

Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-97.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631ATPPROSITE-ProRule annotation
Sitei97 – 971AutoinhibitoryBy similarity
Active sitei161 – 1611Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 489ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. protein phosphorylation Source: UniProtKB
  2. regulation of signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek7 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 7
Short name:
NimA-related protein kinase 7
Gene namesi
Name:Nek7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1890645. Nek7.

Subcellular locationi

  1. Nucleus By similarity
  2. Cytoplasm
  3. Cytoplasmcytoskeletonspindle pole By similarity
  4. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

  5. Note: Present at centrosome throughout the cell cycle. Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. microtubule organizing center Source: UniProtKB-SubCell
  3. nucleoplasm Source: MGI
  4. spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Serine/threonine-protein kinase Nek7PRO_0000086431Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei191 – 1911PhosphothreonineBy similarity
Modified residuei195 – 1951Phosphoserine; by NEK9By similarity
Modified residuei199 – 1991PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylation at Ser-195 required for its activation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9ES74.
PaxDbiQ9ES74.
PRIDEiQ9ES74.

PTM databases

PhosphoSiteiQ9ES74.

Expressioni

Gene expression databases

BgeeiQ9ES74.
ExpressionAtlasiQ9ES74. baseline and differential.
GenevestigatoriQ9ES74.

Interactioni

Subunit structurei

Monomer. Interacts with NEK9 (By similarity).By similarity

Structurei

3D structure databases

SMRiQ9ES74. Positions 21-300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 299266Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni20 – 3314NTE motifBy similarityAdd
BLAST

Domaini

Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9 (By similarity).By similarity
The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000233029.
HOVERGENiHBG105886.
InParanoidiQ9ES74.
KOiK08857.
OMAiQLVNICI.
OrthoDBiEOG74J983.
PhylomeDBiQ9ES74.
TreeFamiTF105135.

Family and domain databases

InterProiIPR016253. Integrin-linked_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000654. Integrin-linked_kinase. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ES74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEQSQGMQG PPVTQFQPQK ALRPDMGYNT LANFRIEKKI GRGQFSEVYR
60 70 80 90 100
ASCLLDGVPV ALKKVQIFDL MDAKARADCI KEIDLLKQLN HPNVIKYYAS
110 120 130 140 150
FIEDNELNIV LELADAGDLS RMIKHFKKQK RLIPERTVWK YFVQLCSALD
160 170 180 190 200
HMHSRRVMHR DIKPANVFIT ATGVVKLGDL GLGRFFSSKT TAAHSLVGTP
210 220 230 240 250
YYMSPERIHE NGYNFKSDIW SLGCLLYEMA ALQSPFYGDK MNLYSLCKKI
260 270 280 290 300
EQCDYPPLPS DHYSEELRQL VNICINPDPE KRPDIAYVYD VAKRMHACTA

ST
Length:302
Mass (Da):34,537
Last modified:March 1, 2001 - v1
Checksum:iC5474CD0E63478EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217650 mRNA. Translation: AAG16652.1.
BC037697 mRNA. Translation: AAH37697.1.
AK035502 mRNA. Translation: BAC29080.1.
AK088173 mRNA. Translation: BAC40190.1.
AK149308 mRNA. Translation: BAE28804.1.
CCDSiCCDS15332.1.
RefSeqiNP_067618.1. NM_021605.3.
UniGeneiMm.143817.

Genome annotation databases

EnsembliENSMUST00000186017; ENSMUSP00000140903; ENSMUSG00000026393.
ENSMUST00000187407; ENSMUSP00000140635; ENSMUSG00000026393.
GeneIDi59125.
KEGGimmu:59125.
UCSCiuc007cvn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217650 mRNA. Translation: AAG16652.1.
BC037697 mRNA. Translation: AAH37697.1.
AK035502 mRNA. Translation: BAC29080.1.
AK088173 mRNA. Translation: BAC40190.1.
AK149308 mRNA. Translation: BAE28804.1.
CCDSiCCDS15332.1.
RefSeqiNP_067618.1. NM_021605.3.
UniGeneiMm.143817.

3D structure databases

SMRiQ9ES74. Positions 21-300.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9ES74.

Proteomic databases

MaxQBiQ9ES74.
PaxDbiQ9ES74.
PRIDEiQ9ES74.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000186017; ENSMUSP00000140903; ENSMUSG00000026393.
ENSMUST00000187407; ENSMUSP00000140635; ENSMUSG00000026393.
GeneIDi59125.
KEGGimmu:59125.
UCSCiuc007cvn.1. mouse.

Organism-specific databases

CTDi140609.
MGIiMGI:1890645. Nek7.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000233029.
HOVERGENiHBG105886.
InParanoidiQ9ES74.
KOiK08857.
OMAiQLVNICI.
OrthoDBiEOG74J983.
PhylomeDBiQ9ES74.
TreeFamiTF105135.

Miscellaneous databases

ChiTaRSiNek7. mouse.
NextBioi314776.
PROiQ9ES74.
SOURCEiSearch...

Gene expression databases

BgeeiQ9ES74.
ExpressionAtlasiQ9ES74. baseline and differential.
GenevestigatoriQ9ES74.

Family and domain databases

InterProiIPR016253. Integrin-linked_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000654. Integrin-linked_kinase. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of two evolutionarily conserved murine kinases (Nek6 and Nek7) related to the fungal mitotic regulator, NIMA."
    Kandli M., Feige E., Chen A., Kilfin G., Motro B.
    Genomics 68:187-196(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Retina, Thymus and Urinary bladder.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
  4. "Nek7 kinase targeting leads to early mortality, cytokinesis disturbance and polyploidy."
    Salem H., Rachmin I., Yissachar N., Cohen S., Amiel A., Haffner R., Lavi L., Motro B.
    Oncogene 29:4046-4057(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNEK7_MOUSE
AccessioniPrimary (citable) accession number: Q9ES74
Secondary accession number(s): Q3UEV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: March 1, 2001
Last modified: April 1, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.