Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Melanoma-associated antigen D1

Gene

Maged1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Plays a role in the circadian rythm regulation. May act as RORA co-regulator, modulating the expression of core clock genes such as ARNTL/BMAL1 and NFIL3, induced, or NR1D1, repressed.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Tumor antigen

Keywords - Biological processi

Biological rhythms, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-associated antigen D1
Alternative name(s):
MAGE-D1 antigen
Neurotrophin receptor-interacting MAGE homolog
Sertoli cell necdin-related gene protein 1
Short name:
SNERG-1
Gene namesi
Name:Maged1
Synonyms:Nrage
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi70898. Maged1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 775775Melanoma-associated antigen D1PRO_0000156726Add
BLAST

Proteomic databases

PaxDbiQ9ES73.
PRIDEiQ9ES73.

PTM databases

iPTMnetiQ9ES73.
PhosphoSiteiQ9ES73.

Expressioni

Tissue specificityi

Ubiquitous and in the seminiferous tubules expressed in Sertoli cells but not in germ cells. Expression decreases in all tissues with increased age and is detectable only in brain cortex and lung.

Developmental stagei

Expressed at low levels throughout the embryo and is enriched in the developing brain and spinal cord.

Inductioni

Follitropin decreased expression while lutropin and prolactin stimulated expression.

Gene expression databases

ExpressionAtlasiQ9ES73. baseline and differential.
GenevisibleiQ9ES73. RN.

Interactioni

Subunit structurei

Interacts with DLX5, DLX7 and MSX2 and forms homomultimers. Interacts with UNC5A. Interacts with TRIM28 and PJA1. Interacts with NGFR/p75NTR and RORA.2 Publications

Protein-protein interaction databases

BioGridi249969. 1 interaction.
STRINGi10116.ENSRNOP00000009870.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati293 – 29861
Repeati299 – 30462
Repeati305 – 31063
Repeati329 – 33464
Repeati335 – 34065
Repeati341 – 34666
Repeati347 – 35267
Repeati353 – 35868
Repeati359 – 36469
Repeati365 – 370610
Repeati371 – 376611
Repeati377 – 382612
Repeati383 – 388613
Repeati389 – 394614
Repeati395 – 400615
Repeati401 – 406616
Repeati407 – 412617
Repeati413 – 418618
Repeati419 – 424619
Repeati425 – 429520; approximate
Repeati430 – 435621
Repeati436 – 441622
Domaini468 – 666199MAGEPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni293 – 44114922 X 6 AA tandem repeats of W-[PQ]-X-P-X-XAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi276 – 462187Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 MAGE domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000049038.
HOVERGENiHBG003714.
InParanoidiQ9ES73.
KOiK12464.
OMAiWQNPVIW.
OrthoDBiEOG75F4GM.
PhylomeDBiQ9ES73.

Family and domain databases

InterProiIPR030083. MAGED1.
IPR002190. MHD_dom.
[Graphical view]
PANTHERiPTHR11736:SF28. PTHR11736:SF28. 1 hit.
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ES73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQKPDGGAG LRGFQAEASV EDSALLVQTL MEAIQISEAP PTSQATAAAS
60 70 80 90 100
GPNASPQSSQ PPTANEKADT EVSAAAARPK TGFKAQNTTT KGPNDYSQAR
110 120 130 140 150
NAKEMPKNQP KVAFKSQNAT SKGPHAASDF SHAASTGKSA AKKSEMAFKG
160 170 180 190 200
QNTTTKAGPS ATYNFTQSPS ANEMTNNQPK TAKAWNDTTK IPGADAQTQN
210 220 230 240 250
VNQAKMADVG TSAGISETDG AAAQTSADGS QAQNVESRTI IRGKRTRKIN
260 270 280 290 300
NLNVEENSNG DQRRASLASG NWRSAPVPVT TQNPPGAPPN VLWQTPLAWQ
310 320 330 340 350
NPSGWQNQTA RQTPPARQSP PARQTPSAWQ NPVAWQNPVI WPNPVIWQNP
360 370 380 390 400
VIWPNPIVWP GPIVWPNPMA WQSTPGWQSP PSWQAPPSWQ SPQDWQGPPD
410 420 430 440 450
WQLPPDWSMP PDWSFPSDWP FPPDWIPADW PIPPDWQNLR PSPNLRSSPN
460 470 480 490 500
SRASQNQGPP QPRDVALLQE RANKLVKYLM LKDYTKVPIK RSEMLRDIIR
510 520 530 540 550
EYTDVYPEII ERACFVLEKK FGIQLKEIDK EEHLYILIST PESLAGILGT
560 570 580 590 600
TKDTPKLGLL LVILGIIFMN GNRATEAVLW EALRKMGLRP GVRHPLLGDL
610 620 630 640 650
RKLLTYEFVK QKYLDYRRVP NSNPPEYEFL WGLRSYHETS KMKVLRFIAE
660 670 680 690 700
VQKRDPRDWT AQFMEAADEA LDALDAAAAE AEARAEARNR MGIGDEAVSG
710 720 730 740 750
PWSWDDIEFE LLTWDEEGDF GDPWSRIPFT FWARYHQNAR SRFPQAFTGP
760 770
IIGPSGTATA NFAANFGAIG FFWVE
Length:775
Mass (Da):85,798
Last modified:May 1, 2007 - v3
Checksum:iF8BF3B170B8A3742
GO

Sequence cautioni

The sequence CAB65381.1 differs from that shown. Reason: Frameshift at position 726. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781Q → R in AAF75283 (Ref. 4) Curated
Sequence conflicti425 – 4273WIP → CIL in CAB65381 (PubMed:11014239).Curated
Sequence conflicti425 – 4273WIP → CIL in AAF75283 (Ref. 4) Curated
Sequence conflicti693 – 6997IGDEAVS → LETELCL in CAB65381 (PubMed:11014239).Curated
Sequence conflicti693 – 6997IGDEAVS → LETELCL in AAF75283 (Ref. 4) Curated
Sequence conflicti724 – 7252WS → GP in AAF75283 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217964 mRNA. Translation: AAG09705.1.
BC081756 mRNA. Translation: AAH81756.1.
AJ133038 mRNA. Translation: CAB65381.1. Frameshift.
AF274043 mRNA. Translation: AAF75283.1.
RefSeqiNP_445861.1. NM_053409.2.
UniGeneiRn.54596.

Genome annotation databases

EnsembliENSRNOT00000009870; ENSRNOP00000009870; ENSRNOG00000006756.
GeneIDi84469.
KEGGirno:84469.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF217964 mRNA. Translation: AAG09705.1.
BC081756 mRNA. Translation: AAH81756.1.
AJ133038 mRNA. Translation: CAB65381.1. Frameshift.
AF274043 mRNA. Translation: AAF75283.1.
RefSeqiNP_445861.1. NM_053409.2.
UniGeneiRn.54596.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249969. 1 interaction.
STRINGi10116.ENSRNOP00000009870.

PTM databases

iPTMnetiQ9ES73.
PhosphoSiteiQ9ES73.

Proteomic databases

PaxDbiQ9ES73.
PRIDEiQ9ES73.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009870; ENSRNOP00000009870; ENSRNOG00000006756.
GeneIDi84469.
KEGGirno:84469.

Organism-specific databases

CTDi9500.
RGDi70898. Maged1.

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000049038.
HOVERGENiHBG003714.
InParanoidiQ9ES73.
KOiK12464.
OMAiWQNPVIW.
OrthoDBiEOG75F4GM.
PhylomeDBiQ9ES73.

Miscellaneous databases

NextBioi616948.
PROiQ9ES73.

Gene expression databases

ExpressionAtlasiQ9ES73. baseline and differential.
GenevisibleiQ9ES73. RN.

Family and domain databases

InterProiIPR030083. MAGED1.
IPR002190. MHD_dom.
[Graphical view]
PANTHERiPTHR11736:SF28. PTHR11736:SF28. 1 hit.
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NRAGE, a novel MAGE protein, interacts with the p75 neurotrophin receptor and facilitates nerve growth factor dependent apoptosis."
    Salehi A.H., Roux P.P., Kubu C.J., Zeindler C., Bhakar A., Tannis L.-L., Verdi J.M., Barker P.A.
    Neuron 27:279-288(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN APOPTOSIS, INTERACTION WITH NGFR.
    Strain: Sprague-Dawley.
    Tissue: Neural crest.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  3. "A novel messenger ribonucleic acid homologous to human MAGE-D is strongly expressed in rat Sertoli cells and weakly in Leydig cells and is regulated by follitropin, lutropin, and prolactin."
    Hennuy B., Reiter E., Cornet A., Bruyninx M., Daukandt M., Houssa P., N'Guyen V.-H., Closset J., Hennen G.
    Endocrinology 141:3821-3831(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 206-775.
    Tissue: Testis.
  4. "Rattus norvegicus mRNA for SNERG-1 protein, partial CDS."
    Zhang C., He F.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 178-775.
  5. "UNC5H1 induces apoptosis via its juxtamembrane region through an interaction with NRAGE."
    Williams M.E., Strickland P., Watanabe K., Hinck L.
    J. Biol. Chem. 278:17483-17490(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UNC5A.

Entry informationi

Entry nameiMAGD1_RAT
AccessioniPrimary (citable) accession number: Q9ES73
Secondary accession number(s): Q66HP3, Q9JHZ6, Q9QX92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: May 1, 2007
Last modified: May 11, 2016
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.