Reviewed, UniProtKB/Swiss-Prot Q9ES71 (GNPAT_RAT)

Names and origin

Protein names

Recommended name:
    Dihydroxyacetone phosphate acyltransferase
      Short name=DHAP-AT
      Short name=DAP-AT
    EC=2.3.1.42
Alternative name(s):
    Glycerone-phosphate O-acyltransferase
    Acyl-CoA:dihydroxyacetonephosphateacyltransferase

Gene names

Name:	Gnpat
Synonyms:	Dhapat

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Acyl-CoA + glycerone phosphate = CoA + acylglycerone phosphate.
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism.
Subunit structure	May be part of an heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT By similarity.
Subcellular location	Peroxisome membrane; Peripheral membrane protein; Matrix side By similarity. Note: Exclusively localized to the lumenal side of the peroxisomal membrane By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Cellular component	Membrane Peroxisome
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	peroxisomal membrane Traceable author statement. Source: RGD
Molecular function	glycerone-phosphate O-acyltransferase activity Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 678	678	Dihydroxyacetone phosphate acyltransferase		PRO_0000195248
Regions
	Motif	161 – 166	6	HXXXXD motif
	Motif	676 – 678	3	Microbody targeting signal Potential
	Compositional bias	5 – 9	5	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	Q9ES71-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F825A2728D534B68	FASTA	678	77,076
	10 20 30 40 50 60 MDVPSSSSSR FSVGSASPSS VLLYAKDLKK WDEFEDLLEE RRQVSDFKFA MKCYTPPLYR 70 80 90 100 110 120 GITPCKPSDI KSIVLNSEEI NYVIKQLSRE SLTGVDVLRE EANEILEEMS HKLRIGAIRF 130 140 150 160 170 180 FAFVLSKVFK QIFSKVCVNE EGIQKLQRAI QEHPVILLPS HRSYIDFLML SFVLYNYDLP 190 200 210 220 230 240 VPVIAAGMDF LGMRVVSELL RMSGAFFMRR TFGGNKLYWA VFSEYVKTML RSGYAPVEFF 250 260 270 280 290 300 LEGTRSRAAK TLTPKFGLLN IVMEPFFKRE VFDTYFVPIS ISYDKILEES LYAYELLGIP 310 320 330 340 350 360 KPKESTTGLL KARRILSENF GSIHVYFGDP VSLRSLAAGR LSRNTYNLVP RCIPQKQPED 370 380 390 400 410 420 VQAFVTEVAY KMQLLQIENL ALSPWLLVVA ILLQNQLCMD FDALVEKTLW LKGLTQVFGG 430 440 450 460 470 480 FLLWPDNKLP EEVVQSSILL HSNLATLVKD QVVLKVDSES SQMVNGLVPQ HIAFLMCSAY 490 500 510 520 530 540 RNQLLNVFAR PSLVAVALHM TPGLRKEDVF SCFSFLRNVF SDEFIFLPGN TLRDFEEGCY 550 560 570 580 590 600 LLCKTEVMQM TGKDIILTDK GNAVLQFLTG LFKPFVESYQ ILSKCLLHEE DYFSEKEYLV 610 620 630 640 650 660 TARKFTRQLL DQDASQCYDA LSSELQKNAL AAFVRLGVVE KNKVDSKYVY YVNGPATSKL 670 EEMLGCKKPI GKPATAKL

References

[1]	"cDNA encoding rat dihydroxyacetonephosphate acyltransferase (DHAP-AT)." Ofman R., Lajmir S., Wanders R.J.A. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF218826 mRNA. Translation: AAG17548.1.
IPI	IPI00191554.
RefSeq	NP_445862.1.
UniGene	Rn.1739
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9ES71.
Genome annotation databases
Ensembl	ENSRNOG00000019205. Rattus norvegicus. [Contig view]
GeneID	84470.
KEGG	rno:84470.
NMPDR	fig|10116.3.peg.15208.
Organism-specific databases
RGD	620179. Gnpat.
Phylogenomic databases
HOVERGEN	Q9ES71.
OMA	Q9ES71. GSIHVYF.
Enzyme and pathway databases
BRENDA	2.3.1.42. 248.
Gene expression databases
ArrayExpress	Q9ES71.
GermOnline	ENSRNOG00000019205. Rattus norvegicus.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	616952.

Entry information

Entry name

GNPAT_RAT

Accession

Primary (citable) accession number: Q9ES71

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)