Q9ES71 (GNPAT_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxyacetone phosphate acyltransferase
Short name=DAP-AT
Short name=DHAP-AT
EC=2.3.1.42

Alternative name(s):
Acyl-CoA:dihydroxyacetonephosphateacyltransferase
Glycerone-phosphate O-acyltransferase

Gene names

Name:	Gnpat
Synonyms:	Dhapat

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Acyl-CoA + glycerone phosphate = CoA + acylglycerone phosphate.
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism.
Subunit structure	May be part of an heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT By similarity.
Subcellular location	Peroxisome membrane; Peripheral membrane protein; Matrix side By similarity. Note: Exclusively localized to the lumenal side of the peroxisomal membrane By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Cellular component	Membrane Peroxisome
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: InterPro response to drug Inferred from expression pattern. Source: RGD response to fatty acid Inferred from expression pattern. Source: RGD response to nutrient Inferred from expression pattern. Source: RGD response to starvation Inferred from expression pattern. Source: RGD
Cellular component	mitochondrion Inferred from direct assay. Source: RGD peroxisomal membrane Traceable author statement. Source: RGD
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: InterPro glycerone-phosphate O-acyltransferase activity Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 678	678	Dihydroxyacetone phosphate acyltransferase		PRO_0000195248
Regions
	Motif	161 – 166	6	HXXXXD motif
	Motif	676 – 678	3	Microbody targeting signal Potential
	Compositional bias	5 – 9	5	Poly-Ser
Amino acid modifications
	Modified residue	641	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ES71 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F825A2728D534B68	FASTA	678	77,076
	10 20 30 40 50 60 MDVPSSSSSR FSVGSASPSS VLLYAKDLKK WDEFEDLLEE RRQVSDFKFA MKCYTPPLYR 70 80 90 100 110 120 GITPCKPSDI KSIVLNSEEI NYVIKQLSRE SLTGVDVLRE EANEILEEMS HKLRIGAIRF 130 140 150 160 170 180 FAFVLSKVFK QIFSKVCVNE EGIQKLQRAI QEHPVILLPS HRSYIDFLML SFVLYNYDLP 190 200 210 220 230 240 VPVIAAGMDF LGMRVVSELL RMSGAFFMRR TFGGNKLYWA VFSEYVKTML RSGYAPVEFF 250 260 270 280 290 300 LEGTRSRAAK TLTPKFGLLN IVMEPFFKRE VFDTYFVPIS ISYDKILEES LYAYELLGIP 310 320 330 340 350 360 KPKESTTGLL KARRILSENF GSIHVYFGDP VSLRSLAAGR LSRNTYNLVP RCIPQKQPED 370 380 390 400 410 420 VQAFVTEVAY KMQLLQIENL ALSPWLLVVA ILLQNQLCMD FDALVEKTLW LKGLTQVFGG 430 440 450 460 470 480 FLLWPDNKLP EEVVQSSILL HSNLATLVKD QVVLKVDSES SQMVNGLVPQ HIAFLMCSAY 490 500 510 520 530 540 RNQLLNVFAR PSLVAVALHM TPGLRKEDVF SCFSFLRNVF SDEFIFLPGN TLRDFEEGCY 550 560 570 580 590 600 LLCKTEVMQM TGKDIILTDK GNAVLQFLTG LFKPFVESYQ ILSKCLLHEE DYFSEKEYLV 610 620 630 640 650 660 TARKFTRQLL DQDASQCYDA LSSELQKNAL AAFVRLGVVE KNKVDSKYVY YVNGPATSKL 670 EEMLGCKKPI GKPATAKL

References

[1]	"cDNA encoding rat dihydroxyacetonephosphate acyltransferase (DHAP-AT)." Ofman R., Lajmir S., Wanders R.J.A. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF218826 mRNA. Translation: AAG17548.1.
IPI	IPI00191554.
RefSeq	NP_445862.1. NM_053410.1.
UniGene	Rn.1739.
3D structure databases
ProteinModelPortal	Q9ES71.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ES71.
PTM databases
PhosphoSite	Q9ES71.
Proteomic databases
PRIDE	Q9ES71.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026617; ENSRNOP00000026617; ENSRNOG00000019205.
GeneID	84470.
KEGG	rno:84470.
NMPDR	fig|10116.3.peg.15208.
UCSC	BC061805. rat.
Organism-specific databases
CTD	8443.
RGD	620179. Gnpat.
Phylogenomic databases
eggNOG	roNOG08043.
GeneTree	ENSGT00520000055570.
HOVERGEN	HBG051749.
InParanoid	Q9ES71.
OMA	PRYIPQK.
OrthoDB	EOG4DJJW5.
PhylomeDB	Q9ES71.
Enzyme and pathway databases
BRENDA	2.3.1.42. 5301.
Gene expression databases
ArrayExpress	Q9ES71.
Genevestigator	Q9ES71.
GermOnline	ENSRNOG00000019205. Rattus norvegicus.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR022284. G3P_O-AcylTrfase. [Graphical view]
KO	K00649.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000437. GPAT_DHAPAT. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	616952.

Entry information

Entry name

GNPAT_RAT

Accession

Primary (citable) accession number: Q9ES71

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	November 16, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program