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Protein

Serine/threonine-protein kinase Nek6

Gene

Nek6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, TRIP4, RPS6KB1 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (By similarity). Phosphorylates STAT3.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-108.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741ATPPROSITE-ProRule annotation
Sitei108 – 1081AutoinhibitoryBy similarity
Active sitei172 – 1721Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi51 – 599ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Cell cycle, Cell division, Chromosome partition, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-MMU-3301854. Nuclear Pore Complex (NPC) Disassembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek6 (EC:2.7.11.1)
Alternative name(s):
Never in mitosis A-related kinase 6
Short name:
NimA-related protein kinase 6
Gene namesi
Name:Nek6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1891638. Nek6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Serine/threonine-protein kinase Nek6PRO_0000086428Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei37 – 371PhosphoserineBy similarity
Modified residuei202 – 2021PhosphothreonineBy similarity
Modified residuei206 – 2061Phosphoserine; by NEK9By similarity
Modified residuei210 – 2101PhosphothreonineBy similarity

Post-translational modificationi

Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9ES70.
MaxQBiQ9ES70.
PaxDbiQ9ES70.
PeptideAtlasiQ9ES70.
PRIDEiQ9ES70.

PTM databases

iPTMnetiQ9ES70.
PhosphoSiteiQ9ES70.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026749.
ExpressionAtlasiQ9ES70. baseline and differential.
GenevisibleiQ9ES70. MM.

Interactioni

Subunit structurei

Interacts with NEK9, predominantly in mitosis. Interacts with KIF11 (via C-terminus). Interacts with APBB1 (via WW domain). Interacts with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7, RPS6KB1 and TRIP4 (By similarity). Interacts with STAT3.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi208542. 3 interactions.
STRINGi10090.ENSMUSP00000049723.

Structurei

3D structure databases

ProteinModelPortaliQ9ES70.
SMRiQ9ES70. Positions 41-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 310266Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4444Interaction with ARHGAP33, ANKRA2, CDC42, PRDX3, RAD26L, RBBP6, RPS7 and TRIP4By similarityAdd
BLAST
Regioni267 – 2704Interaction with APBB1By similarity

Domaini

Displays an autoinhibited conformation: Tyr-108 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9 (By similarity).By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0591. Eukaryota.
ENOG410XNQP. LUCA.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000233029.
HOVERGENiHBG105886.
InParanoidiQ9ES70.
KOiK08857.
OMAiTLADFQI.
OrthoDBiEOG091G0AER.
PhylomeDBiQ9ES70.
TreeFamiTF101021.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ES70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGQPSHMPH GGSPNHLCHA LGPAPPPDPQ RLPNTLSFRC SLADFQIEKK
60 70 80 90 100
IGRGQFSEVY KATCLLDRKT VALKKVQIFE MMDAKARQDC VKEIGLLKQL
110 120 130 140 150
NHPNIIKYLD SFIEDNELNI VLELADAGDL SQMIKYFKKQ KRLIPERTVW
160 170 180 190 200
KYFVQLCSAV EHMHSRRVMH RDIKPANVFI TATGIVKLGD LGLGRFFSSE
210 220 230 240 250
TTAAHSLVGT PYYMSPERIH ENGYNFKSDI WSLGCLLYEM AALQSPFYGD
260 270 280 290 300
KMNLFSLCQK IEQCDYPPLP GEHYSEKLRE LVSMCIYPDP DHRPDIVYVH
310
QVARQMHVWT SST
Length:313
Mass (Da):35,742
Last modified:March 1, 2001 - v1
Checksum:i14A8BC9F2C34397C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti210 – 2101T → A in BAC35995 (PubMed:16141072).Curated
Sequence conflicti244 – 2441Q → R in BAB23676 (PubMed:16141072).Curated
Sequence conflicti246 – 2461P → R in BAC35995 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218847 mRNA. Translation: AAG16653.1.
AK004925 mRNA. Translation: BAB23676.2.
AK011519 mRNA. Translation: BAB27673.2.
AK075717 mRNA. Translation: BAC35907.1.
AK075836 mRNA. Translation: BAC35995.1.
AK086700 mRNA. Translation: BAC39721.1.
AK089919 mRNA. Translation: BAC40995.1.
AK170757 mRNA. Translation: BAE42008.1.
BC019524 mRNA. Translation: AAH19524.1.
CCDSiCCDS16009.1.
RefSeqiNP_001153103.1. NM_001159631.1.
NP_067619.1. NM_021606.3.
XP_006498273.1. XM_006498210.2.
UniGeneiMm.143818.

Genome annotation databases

EnsembliENSMUST00000054234; ENSMUSP00000049723; ENSMUSG00000026749.
ENSMUST00000112895; ENSMUSP00000108516; ENSMUSG00000026749.
GeneIDi59126.
KEGGimmu:59126.
UCSCiuc008jnn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218847 mRNA. Translation: AAG16653.1.
AK004925 mRNA. Translation: BAB23676.2.
AK011519 mRNA. Translation: BAB27673.2.
AK075717 mRNA. Translation: BAC35907.1.
AK075836 mRNA. Translation: BAC35995.1.
AK086700 mRNA. Translation: BAC39721.1.
AK089919 mRNA. Translation: BAC40995.1.
AK170757 mRNA. Translation: BAE42008.1.
BC019524 mRNA. Translation: AAH19524.1.
CCDSiCCDS16009.1.
RefSeqiNP_001153103.1. NM_001159631.1.
NP_067619.1. NM_021606.3.
XP_006498273.1. XM_006498210.2.
UniGeneiMm.143818.

3D structure databases

ProteinModelPortaliQ9ES70.
SMRiQ9ES70. Positions 41-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208542. 3 interactions.
STRINGi10090.ENSMUSP00000049723.

PTM databases

iPTMnetiQ9ES70.
PhosphoSiteiQ9ES70.

Proteomic databases

EPDiQ9ES70.
MaxQBiQ9ES70.
PaxDbiQ9ES70.
PeptideAtlasiQ9ES70.
PRIDEiQ9ES70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054234; ENSMUSP00000049723; ENSMUSG00000026749.
ENSMUST00000112895; ENSMUSP00000108516; ENSMUSG00000026749.
GeneIDi59126.
KEGGimmu:59126.
UCSCiuc008jnn.2. mouse.

Organism-specific databases

CTDi10783.
MGIiMGI:1891638. Nek6.

Phylogenomic databases

eggNOGiKOG0591. Eukaryota.
ENOG410XNQP. LUCA.
GeneTreeiENSGT00760000118997.
HOGENOMiHOG000233029.
HOVERGENiHBG105886.
InParanoidiQ9ES70.
KOiK08857.
OMAiTLADFQI.
OrthoDBiEOG091G0AER.
PhylomeDBiQ9ES70.
TreeFamiTF101021.

Enzyme and pathway databases

ReactomeiR-MMU-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-MMU-3301854. Nuclear Pore Complex (NPC) Disassembly.

Miscellaneous databases

ChiTaRSiNek6. mouse.
PROiQ9ES70.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026749.
ExpressionAtlasiQ9ES70. baseline and differential.
GenevisibleiQ9ES70. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEK6_MOUSE
AccessioniPrimary (citable) accession number: Q9ES70
Secondary accession number(s): Q3TCE9
, Q8C6E6, Q9D0E2, Q9DBI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.