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Protein

Calpain-10

Gene

Capn10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. May play a role in insulin-stimulated glucose uptake (By similarity).By similarity

Catalytic activityi

Broad endopeptidase specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei73 – 731By similarity
Active sitei238 – 2381By similarity
Active sitei263 – 2631By similarity

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: BHF-UCL
  • cytoskeletal protein binding Source: BHF-UCL
  • SNARE binding Source: BHF-UCL

GO - Biological processi

  • actin cytoskeleton reorganization Source: RGD
  • aging Source: RGD
  • cellular response to insulin stimulus Source: InterPro
  • mitochondrion organization Source: RGD
  • mitophagy Source: RGD
  • positive regulation of apoptotic process Source: RGD
  • proteolysis Source: RGD
  • response to glucose Source: RGD
  • response to nutrient levels Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.B30. 5301.

Protein family/group databases

MEROPSiC02.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-10 (EC:3.4.22.-)
Alternative name(s):
Calcium-activated neutral proteinase 10
Short name:
CANP 10
Gene namesi
Name:Capn10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69354. Capn10.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: RGD
  • cytoplasm Source: RGD
  • cytoskeleton Source: RGD
  • cytosol Source: BHF-UCL
  • intracellular membrane-bounded organelle Source: RGD
  • mitochondrial inner membrane Source: RGD
  • mitochondrial intermembrane space Source: RGD
  • mitochondrial matrix Source: RGD
  • mitochondrial outer membrane Source: RGD
  • nucleus Source: RGD
  • plasma membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL6130.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 666666Calpain-10PRO_0000207728Add
BLAST

Proteomic databases

PaxDbiQ9ES66.
PRIDEiQ9ES66.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Interactioni

GO - Molecular functioni

  • cytoskeletal protein binding Source: BHF-UCL
  • SNARE binding Source: BHF-UCL

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067567.

Structurei

3D structure databases

ProteinModelPortaliQ9ES66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 321309Calpain catalyticPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni322 – 488167Domain III 1Add
BLAST
Regioni507 – 648142Domain III 2Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOVERGENiHBG050787.
InParanoidiQ9ES66.
KOiK08579.
PhylomeDBiQ9ES66.

Family and domain databases

InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR028791. CAPN10.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF30. PTHR10183:SF30. 1 hit.
PfamiPF01067. Calpain_III. 2 hits.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 2 hits.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 2 hits.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ES66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAVRAETRA RELFRDAAFP ASDSSLFYNL STPLAQFRED ITWRRPQDIC
60 70 80 90 100
ATPQLFPDNP WEGQVKQGLL GDCWFLCACA ALQKSRHLLD QVFPPGQPGW
110 120 130 140 150
SDQEYQGFFT CRIWQFGHWE EVTIDDRLPC LAGRLCFSRC QREDVFWLPL
160 170 180 190 200
LEKAYAKVHG SYEHLWAGQV ADALVDLTGS LAERWSLKDI RKASGQQDRP
210 220 230 240 250
SGGEHRACQQ LLRLKDQCLL SCSVLSPRAG ARELGEFHAF IISDLQELRS
260 270 280 290 300
QTGQGILLLR IHNPWGRRCW QGLWREGGEG WNQVEPAKES ELLAQLQEGE
310 320 330 340 350
FWVEEEEFLR EFDEVTIGYP VTEAGHLQSL YTEKVLCHTR ALPGAWVTGQ
360 370 380 390 400
SAGGCRNNSC FPCNPKFWLR LLEPSEVCVA VLQRPRRRLV GQTRALAGAS
410 420 430 440 450
PAPVNLPGKD YQAVGLHIWK VEKRKISLPR VLSAPPVAGT ACHAYDREIH
460 470 480 490 500
LRCELSPGYY LAVPSTFLKD VPGQFLLRVF STGKISLSAV RLATKGASPG
510 520 530 540 550
AALPAGEWET VQLQGSWRAG QTAGGSRNFA SYPCNPCLPF SVPEGAGPRY
560 570 580 590 600
IRITLQQHCR LSDSQLHPIG FHVFQVPADG EKQDACSLLL QEPLLSCVPH
610 620 630 640 650
CYAQEVSRLC LLSAGNYRIV PSTYLPDTEG TFTVTIATRI DRQSIHSQEM
660
LGQLLQEVSF MAVMKA
Length:666
Mass (Da):74,527
Last modified:March 1, 2001 - v1
Checksum:iA92263A9C8C4947D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF227909 mRNA. Translation: AAG09736.3.
RefSeqiNP_113861.1. NM_031673.2.
UniGeneiRn.176334.

Genome annotation databases

GeneIDi63834.
KEGGirno:63834.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF227909 mRNA. Translation: AAG09736.3.
RefSeqiNP_113861.1. NM_031673.2.
UniGeneiRn.176334.

3D structure databases

ProteinModelPortaliQ9ES66.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000067567.

Chemistry

ChEMBLiCHEMBL6130.

Protein family/group databases

MEROPSiC02.018.

Proteomic databases

PaxDbiQ9ES66.
PRIDEiQ9ES66.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi63834.
KEGGirno:63834.

Organism-specific databases

CTDi11132.
RGDi69354. Capn10.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOVERGENiHBG050787.
InParanoidiQ9ES66.
KOiK08579.
PhylomeDBiQ9ES66.

Enzyme and pathway databases

BRENDAi3.4.22.B30. 5301.

Miscellaneous databases

PROiQ9ES66.

Family and domain databases

InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR028791. CAPN10.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF30. PTHR10183:SF30. 1 hit.
PfamiPF01067. Calpain_III. 2 hits.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 2 hits.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF49758. SSF49758. 2 hits.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization and expression of calpain 10. A novel ubiquitous calpain with nuclear localization."
    Ma H., Fukiage C., Kim Y.H., Duncan M.K., Reed N.A., Shih M., Azuma M., Shearer T.R.
    J. Biol. Chem. 276:28525-28531(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiCAN10_RAT
AccessioniPrimary (citable) accession number: Q9ES66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.