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Protein

Nuclear protein localization protein 4 homolog

Gene

Nploc4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (PubMed:10811609, PubMed:11740563, PubMed:11781570, PubMed:12411482, PubMed:12644454, PubMed:14636562). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (By similarity).By similarity6 Publications

Pathwayi: proteasomal ubiquitin-dependent pathway

This protein is involved in the pathway proteasomal ubiquitin-dependent pathway, which is part of Protein degradation.
View all proteins of this organism that are known to be involved in the pathway proteasomal ubiquitin-dependent pathway and in Protein degradation.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri580 – 608RanBP2-typePROSITE-ProRule annotationAdd BLAST29

GO - Molecular functioni

  • ATPase binding Source: ParkinsonsUK-UCL
  • metal ion binding Source: UniProtKB-KW
  • ubiquitin binding Source: BHF-UCL
  • ubiquitin protein ligase binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionChaperone
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00144

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear protein localization protein 4 homolog
Short name:
Protein NPL4
Gene namesi
Name:Nploc4
Synonyms:Npl4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620794 Nploc4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000579432 – 608Nuclear protein localization protein 4 homologAdd BLAST607

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei179N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9ES54
PRIDEiQ9ES54

PTM databases

iPTMnetiQ9ES54
PhosphoSitePlusiQ9ES54

Interactioni

Subunit structurei

Heterodimer with UFD1 (PubMed:10811609, PubMed:12644454). The heterodimer binds ubiquitinated proteins (PubMed:12644454). The heterodimer binds to VCP and inhibits Golgi membrane fusion (PubMed:10811609, PubMed:12644454). Interacts with ZFAND2B; probably through VCP (By similarity).By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ATPase binding Source: ParkinsonsUK-UCL
  • ubiquitin binding Source: BHF-UCL
  • ubiquitin protein ligase binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi250817, 8 interactors
CORUMiQ9ES54
DIPiDIP-41365N
IntActiQ9ES54, 11 interactors
MINTiQ9ES54
STRINGi10116.ENSRNOP00000051856

Structurei

Secondary structure

1608
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi587 – 589Combined sources3
Beta strandi601 – 603Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NJ3NMR-A580-608[»]
1Q5WNMR-A580-608[»]
ProteinModelPortaliQ9ES54
SMRiQ9ES54
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ES54

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini226 – 363MPNPROSITE-ProRule annotationAdd BLAST138

Domaini

Binds ubiquitinated proteins via its RanBP2-type zinc finger.1 Publication

Sequence similaritiesi

Belongs to the NPL4 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri580 – 608RanBP2-typePROSITE-ProRule annotationAdd BLAST29

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2834 Eukaryota
COG5100 LUCA
HOGENOMiHOG000008139
HOVERGENiHBG052659
InParanoidiQ9ES54
KOiK14015
PhylomeDBiQ9ES54

Family and domain databases

CDDicd08061 MPN_NPL4, 1 hit
InterProiView protein in InterPro
IPR037518 MPN
IPR016563 Npl4
IPR007717 NPL4_C
IPR024682 Npl4_Ub-like_dom
IPR007716 NPL4_Zn-bd_put
IPR029071 Ubiquitin-like_domsf
IPR001876 Znf_RanBP2
IPR036443 Znf_RanBP2_sf
PfamiView protein in Pfam
PF05021 NPL4, 1 hit
PF11543 UN_NPL4, 1 hit
PF05020 zf-NPL4, 1 hit
PIRSFiPIRSF010052 Polyub_prc_Npl4, 1 hit
SMARTiView protein in SMART
SM00547 ZnF_RBZ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF90209 SSF90209, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit
PS01358 ZF_RANBP2_1, 1 hit
PS50199 ZF_RANBP2_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ES54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR
60 70 80 90 100
NKTGEITASS SKSLHLLKIK HGDLLFLFPS SLAGPSSEME TSTSVGLKAF
110 120 130 140 150
GAPHVVEDEI DQYLSKQDGK IYRSRDPQLC RHGPLGKCVH CVPLEPFDED
160 170 180 190 200
YLNHLEPPVK HMSFHAYIRK LTGGADKGKF VALENISCKI KSGCEGHLPW
210 220 230 240 250
PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF WRKTGNQHFG
260 270 280 290 300
YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA
310 320 330 340 350
SKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK
360 370 380 390 400
HPNICRLSPD GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL
410 420 430 440 450
LPCKDAPELG YAKESSSEQY VPDVFYKDID KFGNEITQLA RPLPVEYLII
460 470 480 490 500
DITTTFPKDP VYTFSISQNP FPIENRDVLG ETQDFHSLAT YLSQNTSSVF
510 520 530 540 550
LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA QTWKKSEQWA
560 570 580 590 600
TIEQLCSTVG VQLPGLHEFG AVGGSARAAT SAMWACQHCT FMNQPGTGHC

EMCSLPRT
Length:608
Mass (Da):68,056
Last modified:January 23, 2007 - v3
Checksum:iF6F57DDEA53594A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF234600 mRNA Translation: AAG27534.1
BC101887 mRNA Translation: AAI01888.1
RefSeqiNP_542144.1, NM_080577.2
UniGeneiRn.217306

Genome annotation databases

GeneIDi140639
KEGGirno:140639

Similar proteinsi

Entry informationi

Entry nameiNPL4_RAT
AccessioniPrimary (citable) accession number: Q9ES54
Secondary accession number(s): Q3T1J3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 133 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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