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Protein

PRA1 family protein 3

Gene

Arl6ip5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates intracellular concentrations of taurine and glutamate (Ref. 5). Negatively modulates SLC1A1/EAAC1 glutamate transport activity by decreasing its affinity for glutamate in a PKC activity-dependent manner (PubMed:11242046). May be involved in membrane traffic (PubMed:11096102).1 Publication2 Publications

GO - Molecular functioni

  • protein C-terminus binding Source: RGD

GO - Biological processi

  • negative regulation of L-glutamate transport Source: UniProtKB
  • negative regulation of transport Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
PRA1 family protein 3
Alternative name(s):
ADP-ribosylation factor-like protein 6-interacting protein 5
Short name:
ARL-6-interacting protein 5
Short name:
Aip-5
GTRAP3-18
Glutamate transporter EAAC1-interacting protein
Prenylated Rab acceptor protein 2
Protein JWa
Gene namesi
Name:Arl6ip5
Synonyms:Gtrap3-18, Jwa, Pra2, Praf3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi708572. Arl6ip5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535CytoplasmicBy similarityAdd
BLAST
Transmembranei36 – 5621HelicalSequence analysisAdd
BLAST
Transmembranei57 – 7721HelicalSequence analysisAdd
BLAST
Topological domaini78 – 9215CytoplasmicBy similarityAdd
BLAST
Transmembranei93 – 11321HelicalSequence analysisAdd
BLAST
Transmembranei115 – 13521HelicalSequence analysisAdd
BLAST
Topological domaini136 – 18853CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytoskeleton Source: UniProtKB-SubCell
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi140 – 15112RLRNL…KLENK → ELENLENELENE: Partial relocalization to Golgi membranes. 1 PublicationAdd
BLAST
Mutagenesisi140 – 1478RLRNLKNK → ALANLANA: No effect. 1 Publication
Mutagenesisi185 – 1851K → A: Partial relocalization to Golgi membranes. 1 Publication
Mutagenesisi187 – 1871R → A: Partial relocalization to Golgi membranes. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188PRA1 family protein 3PRO_0000220885Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9ES40.
PRIDEiQ9ES40.

PTM databases

iPTMnetiQ9ES40.
SwissPalmiQ9ES40.

Expressioni

Tissue specificityi

Ubiquitous. Most abundant in heart and brain.1 Publication

Inductioni

By methyl-beta-cyclodextrin.By similarity

Gene expression databases

GenevisibleiQ9ES40. RN.

Interactioni

Subunit structurei

Homodimer. Heterodimer with ARL6IP1 (By similarity). Forms multimers (PubMed:11242046). Interacts with ARL6 (By similarity). Interacts with prenylated RAB1A and RAB3A (PubMed:11096102). Interacts with SLC1A1/EAAC1 (PubMed:11242046). Does not interact with VAMP1, VAMP2 or VAMP3 (PubMed:11096102).By similarity2 Publications

GO - Molecular functioni

  • protein C-terminus binding Source: RGD

Protein-protein interaction databases

BioGridi249355. 1 interaction.
IntActiQ9ES40. 1 interaction.
MINTiMINT-4998231.
STRINGi10116.ENSRNOP00000010185.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni103 – 11715Required for homodimer formation and heterodimer formation with ARL6IP1By similarityAdd
BLAST
Regioni136 – 18853Targeting to endoplasmic reticulum membrane1 PublicationAdd
BLAST

Sequence similaritiesi

Belongs to the PRA1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4050. Eukaryota.
ENOG4111MUC. LUCA.
HOGENOMiHOG000231363.
HOVERGENiHBG053661.
InParanoidiQ9ES40.
OMAiISNLMYY.
OrthoDBiEOG7VDXQH.
PhylomeDBiQ9ES40.
TreeFamiTF105479.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ES40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVNLAPLRA WDDFFPGSDR FARPDFRDIS KWNNRVVSNL LYYQTNYLVV
60 70 80 90 100
AAMMISVVGF LSPFNMILGG IIVVLVFTGF VWAAHNKDIL RRMKKQYPTA
110 120 130 140 150
FVMVVMLASY FLISMFGGVM VFVFGITFPL LLMFIHASLR LRNLKNKLEN
160 170 180
KMEGIGLKKT PMGIILDALE QQEDSINKFA DYISKARE
Length:188
Mass (Da):21,549
Last modified:March 1, 2001 - v1
Checksum:i6FEA29017333C24D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51L → I in AAF60354 (Ref. 3) Curated
Sequence conflicti34 – 341N → D in AAF60354 (Ref. 3) Curated
Sequence conflicti57 – 571V → I in AAF60354 (Ref. 3) Curated
Sequence conflicti72 – 721I → V in AAF60354 (Ref. 3) Curated
Sequence conflicti89 – 891I → V in AAF60354 (Ref. 3) Curated
Sequence conflicti96 – 961Q → R in AAF60354 (Ref. 3) Curated
Sequence conflicti100 – 1001A → T in AAF60354 (Ref. 3) Curated
Sequence conflicti147 – 1471K → Q in AAF60354 (Ref. 3) Curated
Sequence conflicti159 – 1591K → R in AAF60354 (Ref. 3) Curated
Sequence conflicti165 – 1651I → V in AAF60354 (Ref. 3) Curated
Sequence conflicti174 – 1829DSINKFADY → EGINRLTGC in AAF60354 (Ref. 3) Curated
Sequence conflicti186 – 1872AR → VK in AAF60354 (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240182 mRNA. Translation: AAG28598.1.
AF242391 mRNA. Translation: AAF60354.1.
BC061548 mRNA. Translation: AAH61548.1.
RefSeqiNP_076462.1. NM_023972.3.
UniGeneiRn.64512.

Genome annotation databases

GeneIDi66028.
KEGGirno:66028.
UCSCiRGD:708572. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240182 mRNA. Translation: AAG28598.1.
AF242391 mRNA. Translation: AAF60354.1.
BC061548 mRNA. Translation: AAH61548.1.
RefSeqiNP_076462.1. NM_023972.3.
UniGeneiRn.64512.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249355. 1 interaction.
IntActiQ9ES40. 1 interaction.
MINTiMINT-4998231.
STRINGi10116.ENSRNOP00000010185.

PTM databases

iPTMnetiQ9ES40.
SwissPalmiQ9ES40.

Proteomic databases

PaxDbiQ9ES40.
PRIDEiQ9ES40.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi66028.
KEGGirno:66028.
UCSCiRGD:708572. rat.

Organism-specific databases

CTDi10550.
RGDi708572. Arl6ip5.

Phylogenomic databases

eggNOGiKOG4050. Eukaryota.
ENOG4111MUC. LUCA.
HOGENOMiHOG000231363.
HOVERGENiHBG053661.
InParanoidiQ9ES40.
OMAiISNLMYY.
OrthoDBiEOG7VDXQH.
PhylomeDBiQ9ES40.
TreeFamiTF105479.

Miscellaneous databases

PROiQ9ES40.

Gene expression databases

GenevisibleiQ9ES40. RN.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RAB1A AND RAB3A, LACK OF INTERACTION WITH VAMP1; VAMP2 AND VAMP3, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, MUTAGENESIS OF 140-ARG--LYS-147; 140-ARG--LYS-151; LYS-185 AND ARG-187.
    Tissue: Brain.
  2. "Modulation of the neuronal glutamate transporter EAAC1 by the interacting protein GTRAP3-18."
    Lin C.-L.G., Orlov I., Ruggiero A.M., Dykes-Hoberg M., Lee A., Jackson M., Rothstein J.D.
    Nature 410:84-88(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SLC1A1, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "JWA and its role in the regulation of cell differentiation."
    Xia W., Ye J., Cao H.X., Chen J.F., Zhang Z.D., Zhao H., Shen Q., Lu H., Sheng R.L., Wang C.Y., Xu X.K., Wang X.R., Zhou J.W.
    Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  5. "JWA, a novel microtubule-associated protein, regulates homeostasis of intracellular amino acids in PC12 cells."
    Li A.Q., Li A.P., Mao W.G., Chen H., Huang S., Qi H., Ye J., Zhang Z.D., Wang X.R., Sun F., Zou C., Zhou J.W.
    Chin. Sci. Bull. 48:1828-1834(2003)
    Cited for: SUBCELLULAR LOCATION, FUNCTION.

Entry informationi

Entry nameiPRAF3_RAT
AccessioniPrimary (citable) accession number: Q9ES40
Secondary accession number(s): Q9JKD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.