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Protein

Bile acyl-CoA synthetase

Gene

Slc27a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation.

Catalytic activityi

ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA.1 Publication
ATP + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi292 – 303AMPSequence analysisAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14312
ReactomeiR-RNO-159418 Recycling of bile acids and salts
R-RNO-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-RNO-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol
SABIO-RKiQ9ES38

Names & Taxonomyi

Protein namesi
Recommended name:
Bile acyl-CoA synthetase (EC:6.2.1.7)
Short name:
BACS
Alternative name(s):
Bile acid-CoA ligase
Short name:
BA-CoA ligase
Short name:
BAL
Cholate--CoA ligase
Fatty acid transport protein 5
Short name:
FATP-5
Solute carrier family 27 member 5
Very long-chain acyl-CoA synthetase-related protein
Short name:
VLACS-related
Short name:
VLACSR
Gene namesi
Name:Slc27a5
Synonyms:Acsb, Fatp5, Vlacsr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi708535 Slc27a5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30CytoplasmicBy similarityAdd BLAST30
Transmembranei31 – 51HelicalSequence analysisAdd BLAST21
Transmembranei56 – 76HelicalSequence analysisAdd BLAST21
Topological domaini77 – 690CytoplasmicBy similarityAdd BLAST614

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001932151 – 690Bile acyl-CoA synthetaseAdd BLAST690

Proteomic databases

PaxDbiQ9ES38
PRIDEiQ9ES38

PTM databases

iPTMnetiQ9ES38
PhosphoSitePlusiQ9ES38

Expressioni

Tissue specificityi

Predominantly expressed in liver (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000019626
GenevisibleiQ9ES38 RN

Interactioni

Protein-protein interaction databases

IntActiQ9ES38, 1 interactor
STRINGi10116.ENSRNOP00000026574

Structurei

3D structure databases

ProteinModelPortaliQ9ES38
SMRiQ9ES38
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1179 Eukaryota
ENOG410XQ8T LUCA
GeneTreeiENSGT00550000074420
HOGENOMiHOG000044189
HOVERGENiHBG005642
InParanoidiQ9ES38
KOiK08748
OMAiDCRQHGV
OrthoDBiEOG091G0B76
PhylomeDBiQ9ES38
TreeFamiTF313430

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR030302 FATP5
PANTHERiPTHR43107:SF4 PTHR43107:SF4, 1 hit
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ES38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVWKKLTFL LLSLLLLVGL GQPLWPAATA LALRWFLGDP TCFVLLGLAF
60 70 80 90 100
LGRPWISSWI PHWLSLAAAA LTLSLLPPRP PPELRWLHKD VAFAFKLLFY
110 120 130 140 150
GLNLRRRLNR HPPELFVDAL EQQAQARPDQ VALVCTGSEG CSITNRELNA
160 170 180 190 200
KACQAAWALK AKLKEATIQE DKGATAILVL PSKSISALSV FLGLAKLGCP
210 220 230 240 250
VAWINPHSRG MPLLHSVQSS GASVLIVDPD LQENLEEVLP KLLAENIRCF
260 270 280 290 300
YLGHSSPTPG VEALGAALDA APSDPVPAKL RANIKWKSPA IFIYTSGTTG
310 320 330 340 350
LPKPAILSHE RVIQMSNVLS FCGRTADDVV YNVLPLYHSM GLVLGVLGCL
360 370 380 390 400
QLGATCVLAP KFSASRYWAE CRQYSVTVVL YVGEVLRYLC NVPGQPEDKK
410 420 430 440 450
HTVRFALGNG LRADVWENFQ QRFGPIQIWE LYGSTEGNVG LMNYVGHCGA
460 470 480 490 500
VGKTSCFIRM LTPLELVQFD IETAEPVRDK QGFCIPVETG KPGLLLTKIR
510 520 530 540 550
KNQPFLGYRG SQDETKRKLV ANVRQVGDLY YNTGDVLALD QEGFFYFRDR
560 570 580 590 600
LGDTFRWKGE NVSTREVEGV LSILDFLEEV NVYGVTVPGC EGKVGMAAVK
610 620 630 640 650
LAPGKTFDGQ KLYQHVRSWL PAYATPHFIR IQDSLEITNT YKLVKSQLAR
660 670 680 690
EGFDVGVIAD PLYILDNKAE TFRSLMPDVY QAVCEGTWKL
Length:690
Mass (Da):76,266
Last modified:March 1, 2001 - v1
Checksum:iD5BEB8317758DA59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242189 mRNA Translation: AAG09770.1
BC091147 mRNA Translation: AAH91147.1
RefSeqiNP_077057.1, NM_024143.2
UniGeneiRn.207896

Genome annotation databases

EnsembliENSRNOT00000026575; ENSRNOP00000026574; ENSRNOG00000019626
GeneIDi79111
KEGGirno:79111
UCSCiRGD:708535 rat

Similar proteinsi

Entry informationi

Entry nameiS27A5_RAT
AccessioniPrimary (citable) accession number: Q9ES38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health