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Protein

Bile acyl-CoA synthetase

Gene

Slc27a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation.

Catalytic activityi

ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA.1 Publication
ATP + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi292 – 30312AMPSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14312.
ReactomeiR-RNO-159418. Recycling of bile acids and salts.
R-RNO-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-RNO-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
SABIO-RKQ9ES38.

Names & Taxonomyi

Protein namesi
Recommended name:
Bile acyl-CoA synthetase (EC:6.2.1.7)
Short name:
BACS
Alternative name(s):
Bile acid-CoA ligase
Short name:
BA-CoA ligase
Short name:
BAL
Cholate--CoA ligase
Fatty acid transport protein 5
Short name:
FATP-5
Solute carrier family 27 member 5
Very long-chain acyl-CoA synthetase-related protein
Short name:
VLACS-related
Short name:
VLACSR
Gene namesi
Name:Slc27a5
Synonyms:Acsb, Fatp5, Vlacsr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi708535. Slc27a5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030CytoplasmicBy similarityAdd
BLAST
Transmembranei31 – 5121HelicalSequence analysisAdd
BLAST
Transmembranei56 – 7621HelicalSequence analysisAdd
BLAST
Topological domaini77 – 690614CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • basal plasma membrane Source: Ensembl
  • integral component of endoplasmic reticulum membrane Source: Ensembl
  • intracellular membrane-bounded organelle Source: RGD
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690Bile acyl-CoA synthetasePRO_0000193215Add
BLAST

Proteomic databases

PaxDbiQ9ES38.
PRIDEiQ9ES38.

PTM databases

iPTMnetiQ9ES38.
PhosphoSiteiQ9ES38.

Expressioni

Tissue specificityi

Predominantly expressed in liver (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000019626.
GenevisibleiQ9ES38. RN.

Interactioni

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

IntActiQ9ES38. 1 interaction.
MINTiMINT-4569701.
STRINGi10116.ENSRNOP00000026574.

Structurei

3D structure databases

ProteinModelPortaliQ9ES38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1179. Eukaryota.
ENOG410XQ8T. LUCA.
GeneTreeiENSGT00550000074420.
HOGENOMiHOG000044189.
HOVERGENiHBG005642.
InParanoidiQ9ES38.
KOiK08748.
OMAiCFWDDCR.
OrthoDBiEOG091G0B76.
PhylomeDBiQ9ES38.
TreeFamiTF313430.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR030302. FATP5.
[Graphical view]
PANTHERiPTHR24096:SF99. PTHR24096:SF99. 1 hit.
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ES38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVWKKLTFL LLSLLLLVGL GQPLWPAATA LALRWFLGDP TCFVLLGLAF
60 70 80 90 100
LGRPWISSWI PHWLSLAAAA LTLSLLPPRP PPELRWLHKD VAFAFKLLFY
110 120 130 140 150
GLNLRRRLNR HPPELFVDAL EQQAQARPDQ VALVCTGSEG CSITNRELNA
160 170 180 190 200
KACQAAWALK AKLKEATIQE DKGATAILVL PSKSISALSV FLGLAKLGCP
210 220 230 240 250
VAWINPHSRG MPLLHSVQSS GASVLIVDPD LQENLEEVLP KLLAENIRCF
260 270 280 290 300
YLGHSSPTPG VEALGAALDA APSDPVPAKL RANIKWKSPA IFIYTSGTTG
310 320 330 340 350
LPKPAILSHE RVIQMSNVLS FCGRTADDVV YNVLPLYHSM GLVLGVLGCL
360 370 380 390 400
QLGATCVLAP KFSASRYWAE CRQYSVTVVL YVGEVLRYLC NVPGQPEDKK
410 420 430 440 450
HTVRFALGNG LRADVWENFQ QRFGPIQIWE LYGSTEGNVG LMNYVGHCGA
460 470 480 490 500
VGKTSCFIRM LTPLELVQFD IETAEPVRDK QGFCIPVETG KPGLLLTKIR
510 520 530 540 550
KNQPFLGYRG SQDETKRKLV ANVRQVGDLY YNTGDVLALD QEGFFYFRDR
560 570 580 590 600
LGDTFRWKGE NVSTREVEGV LSILDFLEEV NVYGVTVPGC EGKVGMAAVK
610 620 630 640 650
LAPGKTFDGQ KLYQHVRSWL PAYATPHFIR IQDSLEITNT YKLVKSQLAR
660 670 680 690
EGFDVGVIAD PLYILDNKAE TFRSLMPDVY QAVCEGTWKL
Length:690
Mass (Da):76,266
Last modified:March 1, 2001 - v1
Checksum:iD5BEB8317758DA59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242189 mRNA. Translation: AAG09770.1.
BC091147 mRNA. Translation: AAH91147.1.
RefSeqiNP_077057.1. NM_024143.2.
UniGeneiRn.207896.

Genome annotation databases

EnsembliENSRNOT00000026575; ENSRNOP00000026574; ENSRNOG00000019626.
GeneIDi79111.
KEGGirno:79111.
UCSCiRGD:708535. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF242189 mRNA. Translation: AAG09770.1.
BC091147 mRNA. Translation: AAH91147.1.
RefSeqiNP_077057.1. NM_024143.2.
UniGeneiRn.207896.

3D structure databases

ProteinModelPortaliQ9ES38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9ES38. 1 interaction.
MINTiMINT-4569701.
STRINGi10116.ENSRNOP00000026574.

PTM databases

iPTMnetiQ9ES38.
PhosphoSiteiQ9ES38.

Proteomic databases

PaxDbiQ9ES38.
PRIDEiQ9ES38.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026575; ENSRNOP00000026574; ENSRNOG00000019626.
GeneIDi79111.
KEGGirno:79111.
UCSCiRGD:708535. rat.

Organism-specific databases

CTDi10998.
RGDi708535. Slc27a5.

Phylogenomic databases

eggNOGiKOG1179. Eukaryota.
ENOG410XQ8T. LUCA.
GeneTreeiENSGT00550000074420.
HOGENOMiHOG000044189.
HOVERGENiHBG005642.
InParanoidiQ9ES38.
KOiK08748.
OMAiCFWDDCR.
OrthoDBiEOG091G0B76.
PhylomeDBiQ9ES38.
TreeFamiTF313430.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14312.
ReactomeiR-RNO-159418. Recycling of bile acids and salts.
R-RNO-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-RNO-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
SABIO-RKQ9ES38.

Miscellaneous databases

PROiQ9ES38.

Gene expression databases

BgeeiENSRNOG00000019626.
GenevisibleiQ9ES38. RN.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR030302. FATP5.
[Graphical view]
PANTHERiPTHR24096:SF99. PTHR24096:SF99. 1 hit.
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS27A5_RAT
AccessioniPrimary (citable) accession number: Q9ES38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.