Q9ES28 (ARHG7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rho guanine nucleotide exchange factor 7

Alternative name(s):
Beta-Pix
PAK-interacting exchange factor beta
p85SPR

Gene names

Name:	Arhgef7
Synonyms:	Kiaa0142, Pak3bp

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	862 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. May function as a positive regulator of apoptosis. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons By similarity. Ref.11
Subunit structure	Interacts with PAK kinases through the SH3 domain. Interacts with unphosphorylated PAK1. Interacts with ITCH. Interacts with SCRIB; interaction is direct and may play a role in regulation of apoptosis By similarity. Interacts with GIT1 and TGFB1I1. Interacts with FRMPD4 (via N-terminus). Interacts with CaMK1 By similarity. Interacts with PTK2/FAK1 and RAC1. Ref.8 Ref.9 Ref.11
Subcellular location	Cell junction › focal adhesion. Cell projection › ruffle. Cytoplasm › cell cortex. Note: Detected at cell adhesions. A small proportion is detected at focal adhesions. Ref.11
Tissue specificity	Seems to be expressed in the central nervous system. Isoform B, isoform C and isoform E are expressed with highest levels in brain and testis. Ref.5 Ref.6
Post-translational modification	Phosphorylated on Ser-673 by CaMK1; enhancement of GEF activity and downstream activation of RAC1 By similarity. Phosphorylated by PTK2/FAK1; this promotes interaction with RAC1. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16
Sequence similarities	Contains 1 CH (calponin-homology) domain. Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 SH3 domain.
Sequence caution	The sequence AAB57691.1 differs from that shown. Reason: Erroneous initiation. The sequence AAG18017.1 differs from that shown. Reason: Erroneous initiation. The sequence AAG18018.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH44838.1 differs from that shown. Reason: Erroneous initiation. The sequence AAK97363.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC35033.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC97874.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE23905.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Neurogenesis
Cellular component	Cell junction Cell projection Cytoplasm
Coding sequence diversity	Alternative initiation Alternative splicing
Domain	Coiled coil SH3 domain
Molecular function	Guanine-nucleotide releasing factor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro nervous system development Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB regulation of Rho protein signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	Rho guanyl-nucleotide exchange factor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Git1	Q9Z272	2	EBI-642580,EBI-3842379	From a different organism.
GIT2	Q14161	2	EBI-642580,EBI-1046878	From a different organism.
Git2	Q9JLQ2	6	EBI-642580,EBI-642860

Alternative products

This entry describes 8 isoforms produced by alternative splicing and alternative initiation. [Align] [Select]

Isoform B (identifier: Q9ES28-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: Q9ES28-2)

The sequence of this isoform differs from the canonical sequence as follows:
     712-771: TWQGTDLMHNHVLADDDQSSLDSLGRRSSLSRLEPSDLSEDSEYDSIWTAHSYRMGSASR → S

Isoform C (identifier: Q9ES28-3)

The sequence of this isoform differs from the canonical sequence as follows:
     576-650: Missing.

Isoform D (identifier: Q9ES28-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     53-55: IEK → MLQ
     712-771: TWQGTDLMHNHVLADDDQSSLDSLGRRSSLSRLEPSDLSEDSEYDSIWTAHSYRMGSASR → S

Isoform E (identifier: Q9ES28-5)

Also known as: d;

The sequence of this isoform differs from the canonical sequence as follows:
     772-824: SRKESAPQVLLPEEEKIIVEETKSNGQTVIEEKSLVDTVYALKDEVQELRQDN → KSCCSYISHQN
     825-862: Missing.

Isoform F (identifier: Q9ES28-6)

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     53-55: IEK → MLQ
     712-820: TWQGTDLMHN...YALKDEVQEL → SECRSSPRVGTDYKQLLHGLAALEREVSGA
     821-862: Missing.

Isoform G (identifier: Q9ES28-7)

Also known as: b(L);

The sequence of this isoform differs from the canonical sequence as follows:
     1-52: Missing.
     53-55: IEK → MLQ

Isoform H (identifier: Q9ES28-8)

Also known as: b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-157: Missing.

Note: Produced by alternative initiation at Met-158 of isoform G.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 862

862

Rho guanine nucleotide exchange factor 7

PRO_0000080922

Regions

Domain

1 – 111

111

CH

Domain

163 – 222

60

SH3

Domain

250 – 430

181

DH

Domain

452 – 557

106

PH

Coiled coil

804 – 854

51

Potential

Amino acid modifications

Modified residue

132

1

Phosphoserine Ref.14

Modified residue

155

1

Phosphoserine Ref.13

Modified residue

164

1

Phosphoserine Ref.16

Modified residue

228

1

Phosphoserine By similarity

Modified residue

497

1

Phosphoserine Ref.12 Ref.15

Modified residue

673

1

Phosphoserine; by CaMK1 By similarity

Modified residue

776

1

Phosphoserine Ref.14

Natural variations

Alternative sequence

1 – 157

157

Missing in isoform H.

VSP_023051

Alternative sequence

1 – 52

52

Missing in isoform D, isoform F and isoform G.

VSP_023052

Alternative sequence

53 – 55

3

IEK → MLQ in isoform D, isoform F and isoform G.

VSP_023053

Alternative sequence

576 – 650

75

Missing in isoform C.

VSP_001816

Alternative sequence

712 – 820

109

TWQGT…EVQEL → SECRSSPRVGTDYKQLLHGL AALEREVSGA in isoform F.

VSP_023054

Alternative sequence

712 – 771

60

TWQGT…GSASR → S in isoform A and isoform D.

VSP_001817

Alternative sequence

772 – 824

53

SRKES…LRQDN → KSCCSYISHQN in isoform E.

VSP_023055

Alternative sequence

821 – 862

42

Missing in isoform F.

VSP_023056

Alternative sequence

825 – 862

38

Missing in isoform E.

VSP_023057

Experimental info

Sequence conflict

485 – 488

4

NLLL → KPSV in AAO65479. Ref.2

Sequence conflict

485 – 488

4

NLLL → KPSV in AAG18017. Ref.5

Sequence conflict

485 – 488

4

NLLL → KPSV in AAG18018. Ref.5

Sequence conflict

485 – 488

4

NLLL → KPSV in AAB57691. Ref.7

Sequence conflict

492

1

A → P in AAO65479. Ref.2

Sequence conflict

492

1

A → P in AAG18017. Ref.5

Sequence conflict

492

1

A → P in AAG18018. Ref.5

Sequence conflict

492

1

A → P in AAB57691. Ref.7

Sequence conflict

616

1

S → R in AAO65479. Ref.2

Sequence conflict

616

1

S → R in AAG18017. Ref.5

Sequence conflict

616

1

S → R in AAB57691. Ref.7

Sequence conflict

624 – 625

2

KT → PH in AAB57691. Ref.7

Sequence conflict

628

1

P → A in AAO65479. Ref.2

Sequence conflict

628

1

P → A in AAB57691. Ref.7

Sequence conflict

630

1

S → G in AAB57691. Ref.7

Sequence conflict

633

1

C → W in AAO65479. Ref.2

Sequence conflict

633

1

C → W in AAG18017. Ref.5

Sequence conflict

633

1

C → W in AAB57691. Ref.7

Sequence conflict

635 – 636

2

RP → WT in AAB57691. Ref.7

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

862

Helix Strand Turn

Details...

Beta strand

167 – 172

6

Beta strand

189 – 195

7

Beta strand

199 – 205

7

Beta strand

208 – 213

6

Helix

214 – 216

3

Beta strand

217 – 219

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified February 6, 2007. Version 2. Checksum: 46D61B606B8391B4	FASTA	862	97,056
	10 20 30 40 50 60 MNSAEQTVTW LITLGVLESP KKTISDPEVF LQASLKDGVV LCRLLERLLP GTIEKVYPEP 70 80 90 100 110 120 RNESECLSNI REFLRACGAS LRLETFDAND LYQGQNFNKV LSSLVTLNKV TADIGLGSDS 130 140 150 160 170 180 VCARPSSHRI KSFDSLGSQS SHSRTSKLLQ SQYRSLDMTD NTNSQLVVRA KFNFQQTNED 190 200 210 220 230 240 ELSFSKGDVI HVTRVEEGGW WEGTHNGRTG WFPSNYVREI KPSEKPVSPK SGTLKSPPKG 250 260 270 280 290 300 FDTTAINKSY YNVVLQNILE TEHEYSKELQ SVLSTYLRPL QTSDKLSSAN TSYLMGNLEE 310 320 330 340 350 360 ISSFQQVLVQ SLEECTKSPE AQQRVGGCFL SLMPQMRTLY LAYCANHPSA VSVLTEHSED 370 380 390 400 410 420 LGEFMETKGA SSPGILVLTT GLSKPFMRLD KYPTLLKELE RHMEDYHPDR QDIQKSMTAF 430 440 450 460 470 480 KNLSAQCQEV RKRKELELQI LTEPIRSWEG DDIKTLGSVT YMSQVTIQCA GSEEKNERYL 490 500 510 520 530 540 LLFPNLLLML SASPRMSGFI YQGKLPTTGM TITKLEDSEN HRNAFEISGS MIERILVSCT 550 560 570 580 590 600 SQQDLHEWVE HLQKQTKVTS VSNPTIKPHS VPSHTLPSHP LTPSSKHADS KPVALTPAYH 610 620 630 640 650 660 TLPHPSHHGT PHTTISWGPL EPPKTPKPWS LSCLRPAPPL RPSAALCYKE DLSKSPKTMK 670 680 690 700 710 720 KLLPKRKPER KPSDEEFAVR KSTAALEEDA QILKVIEAYC TSAKTRQTLN STWQGTDLMH 730 740 750 760 770 780 NHVLADDDQS SLDSLGRRSS LSRLEPSDLS EDSEYDSIWT AHSYRMGSAS RSRKESAPQV 790 800 810 820 830 840 LLPEEEKIIV EETKSNGQTV IEEKSLVDTV YALKDEVQEL RQDNKKMKKS LEEEQRARKD 850 860 LEKLVRKVLK NMNDPAWDET NL
	Isoform A [UniParc]. Checksum: C829852E0C7EA8CD	FASTA	803	90,390
	10 20 30 40 50 60 MNSAEQTVTW LITLGVLESP KKTISDPEVF LQASLKDGVV LCRLLERLLP GTIEKVYPEP 70 80 90 100 110 120 RNESECLSNI REFLRACGAS LRLETFDAND LYQGQNFNKV LSSLVTLNKV TADIGLGSDS 130 140 150 160 170 180 VCARPSSHRI KSFDSLGSQS SHSRTSKLLQ SQYRSLDMTD NTNSQLVVRA KFNFQQTNED 190 200 210 220 230 240 ELSFSKGDVI HVTRVEEGGW WEGTHNGRTG WFPSNYVREI KPSEKPVSPK SGTLKSPPKG 250 260 270 280 290 300 FDTTAINKSY YNVVLQNILE TEHEYSKELQ SVLSTYLRPL QTSDKLSSAN TSYLMGNLEE 310 320 330 340 350 360 ISSFQQVLVQ SLEECTKSPE AQQRVGGCFL SLMPQMRTLY LAYCANHPSA VSVLTEHSED 370 380 390 400 410 420 LGEFMETKGA SSPGILVLTT GLSKPFMRLD KYPTLLKELE RHMEDYHPDR QDIQKSMTAF 430 440 450 460 470 480 KNLSAQCQEV RKRKELELQI LTEPIRSWEG DDIKTLGSVT YMSQVTIQCA GSEEKNERYL 490 500 510 520 530 540 LLFPNLLLML SASPRMSGFI YQGKLPTTGM TITKLEDSEN HRNAFEISGS MIERILVSCT 550 560 570 580 590 600 SQQDLHEWVE HLQKQTKVTS VSNPTIKPHS VPSHTLPSHP LTPSSKHADS KPVALTPAYH 610 620 630 640 650 660 TLPHPSHHGT PHTTISWGPL EPPKTPKPWS LSCLRPAPPL RPSAALCYKE DLSKSPKTMK 670 680 690 700 710 720 KLLPKRKPER KPSDEEFAVR KSTAALEEDA QILKVIEAYC TSAKTRQTLN SSSRKESAPQ 730 740 750 760 770 780 VLLPEEEKII VEETKSNGQT VIEEKSLVDT VYALKDEVQE LRQDNKKMKK SLEEEQRARK 790 800 DLEKLVRKVL KNMNDPAWDE TNL
	Isoform C [UniParc]. Checksum: EEB889F6BE45B63D	FASTA	787	88,950
	10 20 30 40 50 60 MNSAEQTVTW LITLGVLESP KKTISDPEVF LQASLKDGVV LCRLLERLLP GTIEKVYPEP 70 80 90 100 110 120 RNESECLSNI REFLRACGAS LRLETFDAND LYQGQNFNKV LSSLVTLNKV TADIGLGSDS 130 140 150 160 170 180 VCARPSSHRI KSFDSLGSQS SHSRTSKLLQ SQYRSLDMTD NTNSQLVVRA KFNFQQTNED 190 200 210 220 230 240 ELSFSKGDVI HVTRVEEGGW WEGTHNGRTG WFPSNYVREI KPSEKPVSPK SGTLKSPPKG 250 260 270 280 290 300 FDTTAINKSY YNVVLQNILE TEHEYSKELQ SVLSTYLRPL QTSDKLSSAN TSYLMGNLEE 310 320 330 340 350 360 ISSFQQVLVQ SLEECTKSPE AQQRVGGCFL SLMPQMRTLY LAYCANHPSA VSVLTEHSED 370 380 390 400 410 420 LGEFMETKGA SSPGILVLTT GLSKPFMRLD KYPTLLKELE RHMEDYHPDR QDIQKSMTAF 430 440 450 460 470 480 KNLSAQCQEV RKRKELELQI LTEPIRSWEG DDIKTLGSVT YMSQVTIQCA GSEEKNERYL 490 500 510 520 530 540 LLFPNLLLML SASPRMSGFI YQGKLPTTGM TITKLEDSEN HRNAFEISGS MIERILVSCT 550 560 570 580 590 600 SQQDLHEWVE HLQKQTKVTS VSNPTIKPHS VPSHTDLSKS PKTMKKLLPK RKPERKPSDE 610 620 630 640 650 660 EFAVRKSTAA LEEDAQILKV IEAYCTSAKT RQTLNSTWQG TDLMHNHVLA DDDQSSLDSL 670 680 690 700 710 720 GRRSSLSRLE PSDLSEDSEY DSIWTAHSYR MGSASRSRKE SAPQVLLPEE EKIIVEETKS 730 740 750 760 770 780 NGQTVIEEKS LVDTVYALKD EVQELRQDNK KMKKSLEEEQ RARKDLEKLV RKVLKNMNDP AWDETNL
	Isoform D [UniParc]. Checksum: 43F22A9DF918F599	FASTA	751	84,698
	10 20 30 40 50 60 MLQVYPEPRN ESECLSNIRE FLRACGASLR LETFDANDLY QGQNFNKVLS SLVTLNKVTA 70 80 90 100 110 120 DIGLGSDSVC ARPSSHRIKS FDSLGSQSSH SRTSKLLQSQ YRSLDMTDNT NSQLVVRAKF 130 140 150 160 170 180 NFQQTNEDEL SFSKGDVIHV TRVEEGGWWE GTHNGRTGWF PSNYVREIKP SEKPVSPKSG 190 200 210 220 230 240 TLKSPPKGFD TTAINKSYYN VVLQNILETE HEYSKELQSV LSTYLRPLQT SDKLSSANTS 250 260 270 280 290 300 YLMGNLEEIS SFQQVLVQSL EECTKSPEAQ QRVGGCFLSL MPQMRTLYLA YCANHPSAVS 310 320 330 340 350 360 VLTEHSEDLG EFMETKGASS PGILVLTTGL SKPFMRLDKY PTLLKELERH MEDYHPDRQD 370 380 390 400 410 420 IQKSMTAFKN LSAQCQEVRK RKELELQILT EPIRSWEGDD IKTLGSVTYM SQVTIQCAGS 430 440 450 460 470 480 EEKNERYLLL FPNLLLMLSA SPRMSGFIYQ GKLPTTGMTI TKLEDSENHR NAFEISGSMI 490 500 510 520 530 540 ERILVSCTSQ QDLHEWVEHL QKQTKVTSVS NPTIKPHSVP SHTLPSHPLT PSSKHADSKP 550 560 570 580 590 600 VALTPAYHTL PHPSHHGTPH TTISWGPLEP PKTPKPWSLS CLRPAPPLRP SAALCYKEDL 610 620 630 640 650 660 SKSPKTMKKL LPKRKPERKP SDEEFAVRKS TAALEEDAQI LKVIEAYCTS AKTRQTLNSS 670 680 690 700 710 720 SRKESAPQVL LPEEEKIIVE ETKSNGQTVI EEKSLVDTVY ALKDEVQELR QDNKKMKKSL 730 740 750 EEEQRARKDL EKLVRKVLKN MNDPAWDETN L
	Isoform E (d) [UniParc]. Checksum: 9B38100E66FFDAD9	FASTA	782	87,673
	10 20 30 40 50 60 MNSAEQTVTW LITLGVLESP KKTISDPEVF LQASLKDGVV LCRLLERLLP GTIEKVYPEP 70 80 90 100 110 120 RNESECLSNI REFLRACGAS LRLETFDAND LYQGQNFNKV LSSLVTLNKV TADIGLGSDS 130 140 150 160 170 180 VCARPSSHRI KSFDSLGSQS SHSRTSKLLQ SQYRSLDMTD NTNSQLVVRA KFNFQQTNED 190 200 210 220 230 240 ELSFSKGDVI HVTRVEEGGW WEGTHNGRTG WFPSNYVREI KPSEKPVSPK SGTLKSPPKG 250 260 270 280 290 300 FDTTAINKSY YNVVLQNILE TEHEYSKELQ SVLSTYLRPL QTSDKLSSAN TSYLMGNLEE 310 320 330 340 350 360 ISSFQQVLVQ SLEECTKSPE AQQRVGGCFL SLMPQMRTLY LAYCANHPSA VSVLTEHSED 370 380 390 400 410 420 LGEFMETKGA SSPGILVLTT GLSKPFMRLD KYPTLLKELE RHMEDYHPDR QDIQKSMTAF 430 440 450 460 470 480 KNLSAQCQEV RKRKELELQI LTEPIRSWEG DDIKTLGSVT YMSQVTIQCA GSEEKNERYL 490 500 510 520 530 540 LLFPNLLLML SASPRMSGFI YQGKLPTTGM TITKLEDSEN HRNAFEISGS MIERILVSCT 550 560 570 580 590 600 SQQDLHEWVE HLQKQTKVTS VSNPTIKPHS VPSHTLPSHP LTPSSKHADS KPVALTPAYH 610 620 630 640 650 660 TLPHPSHHGT PHTTISWGPL EPPKTPKPWS LSCLRPAPPL RPSAALCYKE DLSKSPKTMK 670 680 690 700 710 720 KLLPKRKPER KPSDEEFAVR KSTAALEEDA QILKVIEAYC TSAKTRQTLN STWQGTDLMH 730 740 750 760 770 780 NHVLADDDQS SLDSLGRRSS LSRLEPSDLS EDSEYDSIWT AHSYRMGSAS RKSCCSYISH QN
	Isoform F [UniParc]. Checksum: 78B03ECBB4E8A3F4	FASTA	689	77,189
	10 20 30 40 50 60 MLQVYPEPRN ESECLSNIRE FLRACGASLR LETFDANDLY QGQNFNKVLS SLVTLNKVTA 70 80 90 100 110 120 DIGLGSDSVC ARPSSHRIKS FDSLGSQSSH SRTSKLLQSQ YRSLDMTDNT NSQLVVRAKF 130 140 150 160 170 180 NFQQTNEDEL SFSKGDVIHV TRVEEGGWWE GTHNGRTGWF PSNYVREIKP SEKPVSPKSG 190 200 210 220 230 240 TLKSPPKGFD TTAINKSYYN VVLQNILETE HEYSKELQSV LSTYLRPLQT SDKLSSANTS 250 260 270 280 290 300 YLMGNLEEIS SFQQVLVQSL EECTKSPEAQ QRVGGCFLSL MPQMRTLYLA YCANHPSAVS 310 320 330 340 350 360 VLTEHSEDLG EFMETKGASS PGILVLTTGL SKPFMRLDKY PTLLKELERH MEDYHPDRQD 370 380 390 400 410 420 IQKSMTAFKN LSAQCQEVRK RKELELQILT EPIRSWEGDD IKTLGSVTYM SQVTIQCAGS 430 440 450 460 470 480 EEKNERYLLL FPNLLLMLSA SPRMSGFIYQ GKLPTTGMTI TKLEDSENHR NAFEISGSMI 490 500 510 520 530 540 ERILVSCTSQ QDLHEWVEHL QKQTKVTSVS NPTIKPHSVP SHTLPSHPLT PSSKHADSKP 550 560 570 580 590 600 VALTPAYHTL PHPSHHGTPH TTISWGPLEP PKTPKPWSLS CLRPAPPLRP SAALCYKEDL 610 620 630 640 650 660 SKSPKTMKKL LPKRKPERKP SDEEFAVRKS TAALEEDAQI LKVIEAYCTS AKTRQTLNSS 670 680 ECRSSPRVGT DYKQLLHGLA ALEREVSGA
	Isoform G (b(L)) [UniParc]. Checksum: 61F15448C29152B0	FASTA	810	91,364
	10 20 30 40 50 60 MLQVYPEPRN ESECLSNIRE FLRACGASLR LETFDANDLY QGQNFNKVLS SLVTLNKVTA 70 80 90 100 110 120 DIGLGSDSVC ARPSSHRIKS FDSLGSQSSH SRTSKLLQSQ YRSLDMTDNT NSQLVVRAKF 130 140 150 160 170 180 NFQQTNEDEL SFSKGDVIHV TRVEEGGWWE GTHNGRTGWF PSNYVREIKP SEKPVSPKSG 190 200 210 220 230 240 TLKSPPKGFD TTAINKSYYN VVLQNILETE HEYSKELQSV LSTYLRPLQT SDKLSSANTS 250 260 270 280 290 300 YLMGNLEEIS SFQQVLVQSL EECTKSPEAQ QRVGGCFLSL MPQMRTLYLA YCANHPSAVS 310 320 330 340 350 360 VLTEHSEDLG EFMETKGASS PGILVLTTGL SKPFMRLDKY PTLLKELERH MEDYHPDRQD 370 380 390 400 410 420 IQKSMTAFKN LSAQCQEVRK RKELELQILT EPIRSWEGDD IKTLGSVTYM SQVTIQCAGS 430 440 450 460 470 480 EEKNERYLLL FPNLLLMLSA SPRMSGFIYQ GKLPTTGMTI TKLEDSENHR NAFEISGSMI 490 500 510 520 530 540 ERILVSCTSQ QDLHEWVEHL QKQTKVTSVS NPTIKPHSVP SHTLPSHPLT PSSKHADSKP 550 560 570 580 590 600 VALTPAYHTL PHPSHHGTPH TTISWGPLEP PKTPKPWSLS CLRPAPPLRP SAALCYKEDL 610 620 630 640 650 660 SKSPKTMKKL LPKRKPERKP SDEEFAVRKS TAALEEDAQI LKVIEAYCTS AKTRQTLNST 670 680 690 700 710 720 WQGTDLMHNH VLADDDQSSL DSLGRRSSLS RLEPSDLSED SEYDSIWTAH SYRMGSASRS 730 740 750 760 770 780 RKESAPQVLL PEEEKIIVEE TKSNGQTVIE EKSLVDTVYA LKDEVQELRQ DNKKMKKSLE 790 800 810 EEQRARKDLE KLVRKVLKNM NDPAWDETNL
	Isoform H (b) [UniParc]. Checksum: 60F3F1CA3589637C	FASTA	705	79,696
	10 20 30 40 50 60 MTDNTNSQLV VRAKFNFQQT NEDELSFSKG DVIHVTRVEE GGWWEGTHNG RTGWFPSNYV 70 80 90 100 110 120 REIKPSEKPV SPKSGTLKSP PKGFDTTAIN KSYYNVVLQN ILETEHEYSK ELQSVLSTYL 130 140 150 160 170 180 RPLQTSDKLS SANTSYLMGN LEEISSFQQV LVQSLEECTK SPEAQQRVGG CFLSLMPQMR 190 200 210 220 230 240 TLYLAYCANH PSAVSVLTEH SEDLGEFMET KGASSPGILV LTTGLSKPFM RLDKYPTLLK 250 260 270 280 290 300 ELERHMEDYH PDRQDIQKSM TAFKNLSAQC QEVRKRKELE LQILTEPIRS WEGDDIKTLG 310 320 330 340 350 360 SVTYMSQVTI QCAGSEEKNE RYLLLFPNLL LMLSASPRMS GFIYQGKLPT TGMTITKLED 370 380 390 400 410 420 SENHRNAFEI SGSMIERILV SCTSQQDLHE WVEHLQKQTK VTSVSNPTIK PHSVPSHTLP 430 440 450 460 470 480 SHPLTPSSKH ADSKPVALTP AYHTLPHPSH HGTPHTTISW GPLEPPKTPK PWSLSCLRPA 490 500 510 520 530 540 PPLRPSAALC YKEDLSKSPK TMKKLLPKRK PERKPSDEEF AVRKSTAALE EDAQILKVIE 550 560 570 580 590 600 AYCTSAKTRQ TLNSTWQGTD LMHNHVLADD DQSSLDSLGR RSSLSRLEPS DLSEDSEYDS 610 620 630 640 650 660 IWTAHSYRMG SASRSRKESA PQVLLPEEEK IIVEETKSNG QTVIEEKSLV DTVYALKDEV 670 680 690 700 QELRQDNKKM KKSLEEEQRA RKDLEKLVRK VLKNMNDPAW DETNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed: 14621295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM E). Tissue: Brain.
[2]	"betaPix-b(L), a novel isoform of betaPix, is generated by alternative translation." Rhee S., Yang S.J., Lee S.J., Park D. Biochem. Biophys. Res. Commun. 318:415-421(2004) [PubMed: 15120616] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS G AND H). Strain: ICR. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS D AND F). Strain: C57BL/6J. Tissue: Cerebellum and Lung.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D). Strain: FVB/N. Tissue: Mammary tumor.
[5]	"Molecular cloning of neuronally expressed mouse betaPix isoforms." Kim S., Kim T., Lee D., Park S.-H., Kim H., Park D. Biochem. Biophys. Res. Commun. 272:721-725(2000) [PubMed: 10860822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 59-862 (ISOFORM B), NUCLEOTIDE SEQUENCE [MRNA] OF 150-862 (ISOFORM C), TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Brain.
[6]	"Molecular cloning and characterization of a novel mouse betaPix isoform." Kim T., Park D. Mol. Cells 11:89-94(2001) [PubMed: 11266127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 63-862 (ISOFORM E), TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Brain.
[7]	"Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion." Oh W.K., Yoo J.C., Jo D., Song Y.H., Kim M.G., Park D. Biochem. Biophys. Res. Commun. 235:794-798(1997) [PubMed: 9207241] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 126-862 (ISOFORM A). Tissue: Thymus.
[8]	"A tyrosine-phosphorylated protein that binds to an important regulatory region on the cool family of p21-activated kinase-binding proteins." Bagrodia S., Bailey D., Lenard Z., Hart M., Guan J.L., Premont R.T., Taylor S.J., Cerione R.A. J. Biol. Chem. 274:22393-22400(1999) [PubMed: 10428811] [Abstract] Cited for: INTERACTION WITH GIT1.
[9]	"Hic-5 interacts with GIT1 with a different binding mode from paxillin." Nishiya N., Shirai T., Suzuki W., Nose K. J. Biochem. 132:279-289(2002) [PubMed: 12153727] [Abstract] Cited for: INTERACTION WITH GIT1 AND TGFB1I1.
[10]	"Proteomic analysis of in vivo phosphorylated synaptic proteins." Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I., Blackstock W.P., Choudhary J.S., Grant S.G. J. Biol. Chem. 280:5972-5982(2005) [PubMed: 15572359] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-673, MASS SPECTROMETRY. Tissue: Forebrain.
[11]	"FAK potentiates Rac1 activation and localization to matrix adhesion sites: a role for betaPIX." Chang F., Lemmon C.A., Park D., Romer L.H. Mol. Biol. Cell 18:253-264(2007) [PubMed: 17093062] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, INTERACTION WITH PTK2/FAK1.
[12]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497, MASS SPECTROMETRY. Tissue: Brain cortex.
[13]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155, MASS SPECTROMETRY. Tissue: Liver.
[14]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132; SER-673 AND SER-776, MASS SPECTROMETRY. Tissue: Melanoma.
[15]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497 AND SER-673, MASS SPECTROMETRY. Tissue: Macrophage.
[16]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-164, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
[17]	"Crystal structure of the N-terminal SH3 domain of mouse betaPIX, p21-activated kinase-interacting exchange factor." Li X., Liu X., Sun F., Gao J., Zhou H., Gao G.F., Bartlam M., Rao Z. Biochem. Biophys. Res. Commun. 339:407-414(2006) [PubMed: 16307729] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) OF 164-220.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK129064 mRNA. Translation: BAC97874.1. Different initiation.
AY220301 mRNA. Translation: AAO65479.1.
AK052545 mRNA. Translation: BAC35033.1. Different initiation.
AK139156 mRNA. Translation: BAE23905.1. Different initiation.
BC044838 mRNA. Translation: AAH44838.1. Different initiation.
AF247654 mRNA. Translation: AAG18017.1. Different initiation.
AF247655 mRNA. Translation: AAG18018.1. Different initiation.
AF343877 mRNA. Translation: AAK97363.1. Different initiation.
U96634 mRNA. Translation: AAB57691.1. Different initiation.

IPI

IPI00230704.
IPI00331212.
IPI00387322.
IPI00655136.
IPI00828241.
IPI00828536.
IPI00828723.
IPI00828966.

RefSeq

NP_001106989.1. NM_001113517.1.
NP_001106990.1. NM_001113518.1.
NP_059098.2. NM_017402.4.

UniGene

Mm.244068.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ESW	X-ray	2.01	A/B	164-220	[»]

ProteinModelPortal

Q9ES28.

SMR

Q9ES28. Positions 1-116, 162-560, 803-862.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9ES28. 8 interactions.

MINT

MINT-1786275.

STRING

Q9ES28.

PTM databases

PhosphoSite

Q9ES28.

Proteomic databases

PRIDE

Q9ES28.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000033908; ENSMUSP00000033908; ENSMUSG00000031511.
ENSMUST00000074856; ENSMUSP00000074399; ENSMUSG00000031511.
ENSMUST00000098938; ENSMUSP00000096538; ENSMUSG00000031511.
ENSMUST00000110909; ENSMUSP00000106534; ENSMUSG00000031511.
ENSMUST00000110910; ENSMUSP00000106535; ENSMUSG00000031511.
ENSMUST00000110911; ENSMUSP00000106536; ENSMUSG00000031511.
ENSMUST00000110914; ENSMUSP00000106539; ENSMUSG00000031511.

GeneID

54126.

KEGG

mmu:54126.

UCSC

uc009kvu.2. mouse.
uc009kvv.2. mouse.
uc009kvw.2. mouse.
uc009kvx.2. mouse.

Organism-specific databases

CTD

8874.

MGI

MGI:1860493. Arhgef7.

Rouge

Search...

Phylogenomic databases

eggNOG

roNOG08839.

GeneTree

ENSGT00600000084055.

HOVERGEN

HBG050569.

InParanoid

Q9ES28.

OMA

TEAIRCW.

Gene expression databases

ArrayExpress

Q9ES28.

Bgee

Q9ES28.

CleanEx

MM_ARHGEF7.

Genevestigator

Q9ES28.

GermOnline

ENSMUSG00000031511. Mus musculus.

Family and domain databases

InterPro

IPR001715. CH-domain.
IPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]

Gene3D

G3DSA:1.10.418.10. Calponin-homology. 1 hit.
G3DSA:2.30.29.30. PH_type. 1 hit.
G3DSA:1.20.900.10. RhoGEF. 1 hit.

KO

K13710.

Pfam

PF00307. CH. 1 hit.
PF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]

PRINTS

PR00452. SH3DOMAIN.

SMART

SM00033. CH. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF47576. Calponin-homology. 1 hit.
SSF48065. DH-domain. 1 hit.
SSF50044. SH3. 1 hit.

PROSITE

PS50021. CH. 1 hit.
PS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

310921.

SOURCE

Search...

Entry information

Entry name

ARHG7_MOUSE

Accession

Primary (citable) accession number: Q9ES28
Secondary accession number(s): O08757 , Q3UTS5, Q6XPA5, Q6ZQI5, Q8C750, Q91ZZ6, Q9ES27

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program