Q9ES03 (TBX20_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
T-box transcription factor TBX20
Short name=T-box protein 20

Gene names

Name:	Tbx20
Synonyms:	Tbx12

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a transcriptional activator and repressor required for cardiac development and may have key roles in the maintenance of functional and structural phenotypes in adult heart. Ref.6
Subcellular location	Nucleus Potential.
Tissue specificity	Prominently expressed in the extraembryonic mesoderm, developing heart, eye analage and motor neurons of hindbrain and spinal cord. Expressed in extraembryonic tissues such as the amnion and allantois. Ref.3
Developmental stage	Between E7.25 and E8, expression detected in the extraembryonic mesoderm and contributes to amnion and chorion. Allantois expression persists until E10.5 and continues into the umbilical cord. Expression is found throughout heart development. At E7.5, detected in the cardiogenic mesoderm, at E8.0-8.5, found in the cardiac crescent and looping heart tube and from E9.5, found in the forming four-chambered heart. At all stages, expression is much stronger in the myocardium than in the endocardium and expression extends from the cardiogenic into the lateral plate mesoderm. From E10.5 on, weakly expressed in the periphery of the liver lobes, and in cells surrounding the aorta in the urogenital system. At E9.0, weakly expressed in the dorsal half of the optic vesicle. Later, expression spreads ventrally to enclose the entire neural retina at E11.5. At E9.0, expression is initiated in the hindbrain: first in the ventral region of rhombomere (r) 2 and 4, then in r7, r8 and in the cervical spinal cord. By E10.5, two ventral stripes of Tbx20 containing cells are continuous from r2 into the cervical spinal cord and expression is seen in two symmetrical patches of cell bodies in the mantle region of the ventral neural tube. At E11.5, expression follows the migration of motor neurons. Ref.3
Disruption phenotype	Embryos die at mid-gestation. The heart tube of deficient mices does not elongate, but anterior and secondary heart field markers are not affected. Ref.5
Sequence similarities	Contains 1 T-box DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aortic valve development Inferred from mutant phenotype PubMed 15843409. Source: BHF-UCL aortic valve morphogenesis Inferred from electronic annotation. Source: Compara atrial septum morphogenesis Inferred from electronic annotation. Source: Compara blood circulation Inferred from mutant phenotype PubMed 15843414. Source: MGI cardiac chamber formation Inferred from mutant phenotype Ref.5. Source: BHF-UCL cardiac muscle tissue morphogenesis Inferred from mutant phenotype PubMed 15843409. Source: BHF-UCL cardiac right ventricle morphogenesis Inferred from mutant phenotype PubMed 15843409. Source: BHF-UCL cell proliferation Inferred from mutant phenotype PubMed 15843407. Source: MGI dorsal/ventral pattern formation Inferred from mutant phenotype Ref.5. Source: MGI embryonic heart tube elongation Inferred from mutant phenotype Ref.5. Source: BHF-UCL endocardial cushion formation Inferred from mutant phenotype PubMed 15843409. Source: BHF-UCL foramen ovale closure Inferred from electronic annotation. Source: Compara heart looping Inferred from mutant phenotype PubMed 15843409. Source: BHF-UCL muscle contraction Inferred from mutant phenotype PubMed 15843414. Source: MGI negative regulation of SMAD protein complex assembly Inferred from direct assay PubMed 19661464. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.5. Source: BHF-UCL neuron migration Inferred from mutant phenotype PubMed 17119020. Source: MGI outflow tract septum morphogenesis Inferred from mutant phenotype PubMed 15843409. Source: BHF-UCL patterning of blood vessels Inferred from mutant phenotype PubMed 15843414. Source: MGI pericardium morphogenesis Inferred from mutant phenotype Ref.5. Source: BHF-UCL positive regulation of cardiac muscle cell proliferation Inferred from mutant phenotype Ref.5. Source: BHF-UCL pulmonary valve formation Inferred from mutant phenotype PubMed 15843409. Source: BHF-UCL pulmonary vein morphogenesis Inferred from electronic annotation. Source: Compara regulation of epithelial to mesenchymal transition Non-traceable author statement PubMed 15843409. Source: BHF-UCL tricuspid valve development Inferred from mutant phenotype PubMed 15843409. Source: BHF-UCL visceral motor neuron differentiation Inferred from mutant phenotype PubMed 15843409. Source: BHF-UCL
Cellular_component	cytoplasm Inferred from direct assay PubMed 14550786. Source: BHF-UCL nucleus Inferred from direct assay PubMed 14550786. Source: BHF-UCL
Molecular_function	RNA polymerase II regulatory region sequence-specific DNA binding Inferred from direct assay PubMed 14550786. Source: BHF-UCL RNA polymerase II transcription coactivator activity Inferred from direct assay PubMed 14550786. Source: BHF-UCL sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 14978031PubMed 15843407. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9ES03-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9ES03-2)

The sequence of this isoform differs from the canonical sequence as follows:
     298-445: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	T-box transcription factor TBX20		PRO_0000184452
Regions
	DNA binding	108 – 287	180	T-box
	Compositional bias	68 – 72	5	Poly-Gly
	Compositional bias	73 – 78	6	Poly-Ser
	Compositional bias	349 – 353	5	Poly-Ser
Natural variations
	Alternative sequence	298 – 445	148	Missing in isoform 2.		VSP_021730
Experimental info
	Sequence conflict	62	1	D → N in AAG15491. Ref.1
	Sequence conflict	117	1	T → P in AAG15491. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 28, 2006. Version 3. Checksum: 4EAEED0DF0329E57	FASTA	445	49,096
	10 20 30 40 50 60 MEFTASPKPQ LSSRANAFSI AALMSSGGPK EKEAAENTIK PLEQFVEKSS CAQPLGELTS 70 80 90 100 110 120 LDAHAEFGGG GGSPSSSSLC TEPLIPTTPI IPSEEMAKIA CSLETKELWD KFHELGTEMI 130 140 150 160 170 180 ITKSGRRMFP TIRVSFSGVD PESKYIVLMD IVPVDNKRYR YAYHRSSWLV AGKADPPLPA 190 200 210 220 230 240 RLYVHPDSPF TGEQLLKQMV SFEKVKLTNN ELDQHGHIIL NSMHKYQPRV HIIKKKDHTA 250 260 270 280 290 300 SLLNLKSEEF RTFIFPETVF TAVTAYQNQL ITKLKIDSNP FAKGFRDSSR LTDIERESVE 310 320 330 340 350 360 SLIQKHSYAR SPIRTYGEED VLGEESQTTQ SRGSAFTTSD NLSLSSWVSS SSSFPGFQHP 370 380 390 400 410 420 QPLTALGTST ASIATPIPHP IQGSLPPYSR LGMPLTPSAI ASSMQGSGPT FPSFHMPRYH 430 440 HYFQQGPYAA IQGLRHSSAV MTPFV
	Isoform 2 [UniParc]. Checksum: D879B42E2505349D	FASTA	297	33,203
	10 20 30 40 50 60 MEFTASPKPQ LSSRANAFSI AALMSSGGPK EKEAAENTIK PLEQFVEKSS CAQPLGELTS 70 80 90 100 110 120 LDAHAEFGGG GGSPSSSSLC TEPLIPTTPI IPSEEMAKIA CSLETKELWD KFHELGTEMI 130 140 150 160 170 180 ITKSGRRMFP TIRVSFSGVD PESKYIVLMD IVPVDNKRYR YAYHRSSWLV AGKADPPLPA 190 200 210 220 230 240 RLYVHPDSPF TGEQLLKQMV SFEKVKLTNN ELDQHGHIIL NSMHKYQPRV HIIKKKDHTA 250 260 270 280 290 SLLNLKSEEF RTFIFPETVF TAVTAYQNQL ITKLKIDSNP FAKGFRDSSR LTDIERE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tbx12, a novel T-box gene, is expressed during early stages of heart and retinal development." Carson C.T., Kinzler E.R., Parr B.A. Mech. Dev. 96:137-140(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Characterization of the human TBX20 gene, a new member of the T-box gene family closely related to the Drosophila H15 gene." Meins M., Henderson D.J., Bhattacharya S.S., Sowden J.C. Genomics 67:317-332(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Fetal heart.
[3]	"Cloning and expression analysis of the mouse T-box gene tbx20." Kraus F., Haenig B., Kispert A. Mech. Dev. 100:87-91(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: NIH Swiss. Tissue: Embryonic heart.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J.
[5]	"Tbx20 is essential for cardiac chamber differentiation and repression of Tbx2." Singh M.K., Christoffels V.M., Dias J.M., Trowe M.O., Petry M., Schuster-Gossler K., Burger A., Ericson J., Kispert A. Development 132:2697-2707(2005) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[6]	"Dual transcriptional activator and repressor roles of TBX20 regulate adult cardiac structure and function." Sakabe N.J., Aneas I., Shen T., Shokri L., Park S.Y., Bulyk M.L., Evans S.M., Nobrega M.A. Hum. Mol. Genet. 21:2194-2204(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF260557 mRNA. Translation: AAG15491.1. AJ277486 mRNA. Translation: CAC04520.1. AF306667 mRNA. Translation: AAG48599.1. AK141251 mRNA. Translation: BAE24617.1.
IPI	IPI00110539. IPI00275436.
RefSeq	NP_001192014.1. NM_001205085.1. NP_065242.1. NM_020496.3. NP_919239.1. NM_194263.2.
UniGene	Mm.31452.
3D structure databases
ProteinModelPortal	Q9ES03.
SMR	Q9ES03. Positions 99-287.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000052591.
Proteomic databases
PRIDE	Q9ES03.
Protocols and materials databases
DNASU	57246.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000052946; ENSMUSP00000052591; ENSMUSG00000031965. ENSMUST00000166018; ENSMUSP00000126318; ENSMUSG00000031965.
GeneID	57246.
KEGG	mmu:57246.
UCSC	uc009ope.1. mouse. uc009opf.1. mouse.
Organism-specific databases
CTD	57057.
MGI	MGI:1888496. Tbx20.
Phylogenomic databases
eggNOG	NOG272513.
GeneTree	ENSGT00690000102019.
HOGENOM	HOG000286000.
HOVERGEN	HBG067710.
InParanoid	Q9ES03.
KO	K10185.
OMA	RHSSTVM.
OrthoDB	EOG405S14.
Gene expression databases
Bgee	Q9ES03.
CleanEx	MM_TBX20.
Genevestigator	Q9ES03.
GermOnline	ENSMUSG00000031965. Mus musculus.
Family and domain databases
Gene3D	2.60.40.820. 1 hit.
InterPro	IPR008967. p53-like_TF_DNA-bd. IPR001699. TF_T-box. IPR018186. TF_T-box_CS. [Graphical view]
PANTHER	PTHR11267. PTHR11267. 1 hit.
Pfam	PF00907. T-box. 1 hit. [Graphical view]
PRINTS	PR00937. TBOX.
SMART	SM00425. TBOX. 1 hit. [Graphical view]
SUPFAM	SSF49417. P53_like_DNA_bnd. 1 hit.
PROSITE	PS01283. TBOX_1. 1 hit. PS01264. TBOX_2. 1 hit. PS50252. TBOX_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	313513.
SOURCE	Search...

Entry information

Entry name

TBX20_MOUSE

Accession

Primary (citable) accession number: Q9ES03
Secondary accession number(s): Q9EPZ5, Q9ESX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 28, 2006
Last modified:	April 3, 2013
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program