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Protein

T-box transcription factor TBX20

Gene

Tbx20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a transcriptional activator and repressor required for cardiac development and may have key roles in the maintenance of functional and structural phenotypes in adult heart.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi108 – 287180T-boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: MGI
  • RNA polymerase II activating transcription factor binding Source: BHF-UCL
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: BHF-UCL
  • RNA polymerase II transcription coactivator activity Source: BHF-UCL
  • RNA polymerase II transcription factor binding Source: BHF-UCL
  • sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  • aortic valve development Source: BHF-UCL
  • aortic valve morphogenesis Source: MGI
  • atrial septum morphogenesis Source: MGI
  • blood circulation Source: MGI
  • cardiac chamber formation Source: BHF-UCL
  • cardiac muscle tissue morphogenesis Source: BHF-UCL
  • cardiac right ventricle morphogenesis Source: BHF-UCL
  • cardiac septum development Source: BHF-UCL
  • cell proliferation Source: MGI
  • dorsal/ventral pattern formation Source: MGI
  • embryonic heart tube development Source: MGI
  • embryonic heart tube elongation Source: BHF-UCL
  • embryonic heart tube morphogenesis Source: BHF-UCL
  • endocardial cushion formation Source: BHF-UCL
  • endocardial cushion morphogenesis Source: BHF-UCL
  • foramen ovale closure Source: MGI
  • heart looping Source: BHF-UCL
  • muscle contraction Source: MGI
  • negative regulation of SMAD protein complex assembly Source: MGI
  • negative regulation of transcription, DNA-templated Source: BHF-UCL
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • neuron migration Source: MGI
  • outflow tract morphogenesis Source: BHF-UCL
  • outflow tract septum morphogenesis Source: BHF-UCL
  • patterning of blood vessels Source: MGI
  • pericardium morphogenesis Source: BHF-UCL
  • positive regulation of cardiac muscle cell proliferation Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • pulmonary valve formation Source: BHF-UCL
  • pulmonary vein morphogenesis Source: MGI
  • regulation of epithelial to mesenchymal transition Source: BHF-UCL
  • transcription from RNA polymerase II promoter Source: GOC
  • tricuspid valve development Source: BHF-UCL
  • visceral motor neuron differentiation Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
T-box transcription factor TBX20
Short name:
T-box protein 20
Gene namesi
Name:Tbx20
Synonyms:Tbx12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1888496. Tbx20.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryos die at mid-gestation. The heart tube of deficient mices does not elongate, but anterior and secondary heart field markers are not affected.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445T-box transcription factor TBX20PRO_0000184452Add
BLAST

Proteomic databases

PRIDEiQ9ES03.

Expressioni

Tissue specificityi

Prominently expressed in the extraembryonic mesoderm, developing heart, eye analage and motor neurons of hindbrain and spinal cord. Expressed in extraembryonic tissues such as the amnion and allantois.1 Publication

Developmental stagei

Between E7.25 and E8, expression detected in the extraembryonic mesoderm and contributes to amnion and chorion. Allantois expression persists until E10.5 and continues into the umbilical cord. Expression is found throughout heart development. At E7.5, detected in the cardiogenic mesoderm, at E8.0-8.5, found in the cardiac crescent and looping heart tube and from E9.5, found in the forming four-chambered heart. At all stages, expression is much stronger in the myocardium than in the endocardium and expression extends from the cardiogenic into the lateral plate mesoderm. From E10.5 on, weakly expressed in the periphery of the liver lobes, and in cells surrounding the aorta in the urogenital system. At E9.0, weakly expressed in the dorsal half of the optic vesicle. Later, expression spreads ventrally to enclose the entire neural retina at E11.5. At E9.0, expression is initiated in the hindbrain: first in the ventral region of rhombomere (r) 2 and 4, then in r7, r8 and in the cervical spinal cord. By E10.5, two ventral stripes of Tbx20 containing cells are continuous from r2 into the cervical spinal cord and expression is seen in two symmetrical patches of cell bodies in the mantle region of the ventral neural tube. At E11.5, expression follows the migration of motor neurons.1 Publication

Gene expression databases

BgeeiQ9ES03.
CleanExiMM_TBX20.
GenevisibleiQ9ES03. MM.

Interactioni

Protein-protein interaction databases

BioGridi208216. 1 interaction.
STRINGi10090.ENSMUSP00000052591.

Structurei

3D structure databases

ProteinModelPortaliQ9ES03.
SMRiQ9ES03. Positions 99-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi68 – 725Poly-Gly
Compositional biasi73 – 786Poly-Ser
Compositional biasi349 – 3535Poly-Ser

Sequence similaritiesi

Contains 1 T-box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG272513.
GeneTreeiENSGT00760000118897.
HOGENOMiHOG000286000.
HOVERGENiHBG067710.
InParanoidiQ9ES03.
KOiK10185.
OMAiTVMTPFV.
OrthoDBiEOG7GN2PM.
PhylomeDBiQ9ES03.
TreeFamiTF106341.

Family and domain databases

Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
[Graphical view]
PRINTSiPR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ES03-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEFTASPKPQ LSSRANAFSI AALMSSGGPK EKEAAENTIK PLEQFVEKSS
60 70 80 90 100
CAQPLGELTS LDAHAEFGGG GGSPSSSSLC TEPLIPTTPI IPSEEMAKIA
110 120 130 140 150
CSLETKELWD KFHELGTEMI ITKSGRRMFP TIRVSFSGVD PESKYIVLMD
160 170 180 190 200
IVPVDNKRYR YAYHRSSWLV AGKADPPLPA RLYVHPDSPF TGEQLLKQMV
210 220 230 240 250
SFEKVKLTNN ELDQHGHIIL NSMHKYQPRV HIIKKKDHTA SLLNLKSEEF
260 270 280 290 300
RTFIFPETVF TAVTAYQNQL ITKLKIDSNP FAKGFRDSSR LTDIERESVE
310 320 330 340 350
SLIQKHSYAR SPIRTYGEED VLGEESQTTQ SRGSAFTTSD NLSLSSWVSS
360 370 380 390 400
SSSFPGFQHP QPLTALGTST ASIATPIPHP IQGSLPPYSR LGMPLTPSAI
410 420 430 440
ASSMQGSGPT FPSFHMPRYH HYFQQGPYAA IQGLRHSSAV MTPFV
Length:445
Mass (Da):49,096
Last modified:November 28, 2006 - v3
Checksum:i4EAEED0DF0329E57
GO
Isoform 2 (identifier: Q9ES03-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     298-445: Missing.

Show »
Length:297
Mass (Da):33,203
Checksum:iD879B42E2505349D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti62 – 621D → N in AAG15491 (PubMed:10940636).Curated
Sequence conflicti117 – 1171T → P in AAG15491 (PubMed:10940636).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei298 – 445148Missing in isoform 2. 2 PublicationsVSP_021730Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF260557 mRNA. Translation: AAG15491.1.
AJ277486 mRNA. Translation: CAC04520.1.
AF306667 mRNA. Translation: AAG48599.1.
AK141251 mRNA. Translation: BAE24617.1.
CCDSiCCDS22933.1. [Q9ES03-1]
CCDS52744.1. [Q9ES03-2]
RefSeqiNP_001192014.1. NM_001205085.1.
NP_065242.1. NM_020496.3. [Q9ES03-2]
NP_919239.1. NM_194263.2. [Q9ES03-1]
UniGeneiMm.31452.

Genome annotation databases

EnsembliENSMUST00000052946; ENSMUSP00000052591; ENSMUSG00000031965. [Q9ES03-1]
ENSMUST00000166018; ENSMUSP00000126318; ENSMUSG00000031965. [Q9ES03-2]
GeneIDi57246.
KEGGimmu:57246.
UCSCiuc009ope.2. mouse. [Q9ES03-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF260557 mRNA. Translation: AAG15491.1.
AJ277486 mRNA. Translation: CAC04520.1.
AF306667 mRNA. Translation: AAG48599.1.
AK141251 mRNA. Translation: BAE24617.1.
CCDSiCCDS22933.1. [Q9ES03-1]
CCDS52744.1. [Q9ES03-2]
RefSeqiNP_001192014.1. NM_001205085.1.
NP_065242.1. NM_020496.3. [Q9ES03-2]
NP_919239.1. NM_194263.2. [Q9ES03-1]
UniGeneiMm.31452.

3D structure databases

ProteinModelPortaliQ9ES03.
SMRiQ9ES03. Positions 99-287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208216. 1 interaction.
STRINGi10090.ENSMUSP00000052591.

Proteomic databases

PRIDEiQ9ES03.

Protocols and materials databases

DNASUi57246.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052946; ENSMUSP00000052591; ENSMUSG00000031965. [Q9ES03-1]
ENSMUST00000166018; ENSMUSP00000126318; ENSMUSG00000031965. [Q9ES03-2]
GeneIDi57246.
KEGGimmu:57246.
UCSCiuc009ope.2. mouse. [Q9ES03-1]

Organism-specific databases

CTDi57057.
MGIiMGI:1888496. Tbx20.

Phylogenomic databases

eggNOGiNOG272513.
GeneTreeiENSGT00760000118897.
HOGENOMiHOG000286000.
HOVERGENiHBG067710.
InParanoidiQ9ES03.
KOiK10185.
OMAiTVMTPFV.
OrthoDBiEOG7GN2PM.
PhylomeDBiQ9ES03.
TreeFamiTF106341.

Miscellaneous databases

NextBioi313513.
PROiQ9ES03.
SOURCEiSearch...

Gene expression databases

BgeeiQ9ES03.
CleanExiMM_TBX20.
GenevisibleiQ9ES03. MM.

Family and domain databases

Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
[Graphical view]
PRINTSiPR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Tbx12, a novel T-box gene, is expressed during early stages of heart and retinal development."
    Carson C.T., Kinzler E.R., Parr B.A.
    Mech. Dev. 96:137-140(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "Characterization of the human TBX20 gene, a new member of the T-box gene family closely related to the Drosophila H15 gene."
    Meins M., Henderson D.J., Bhattacharya S.S., Sowden J.C.
    Genomics 67:317-332(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Fetal heart.
  3. "Cloning and expression analysis of the mouse T-box gene tbx20."
    Kraus F., Haenig B., Kispert A.
    Mech. Dev. 100:87-91(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: NIH Swiss.
    Tissue: Embryonic heart.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
  5. "Tbx20 is essential for cardiac chamber differentiation and repression of Tbx2."
    Singh M.K., Christoffels V.M., Dias J.M., Trowe M.O., Petry M., Schuster-Gossler K., Burger A., Ericson J., Kispert A.
    Development 132:2697-2707(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. "Dual transcriptional activator and repressor roles of TBX20 regulate adult cardiac structure and function."
    Sakabe N.J., Aneas I., Shen T., Shokri L., Park S.Y., Bulyk M.L., Evans S.M., Nobrega M.A.
    Hum. Mol. Genet. 21:2194-2204(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTBX20_MOUSE
AccessioniPrimary (citable) accession number: Q9ES03
Secondary accession number(s): Q9EPZ5, Q9ESX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 28, 2006
Last modified: July 22, 2015
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.