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Q9ES00

- UBE4B_MOUSE

UniProt

Q9ES00 - UBE4B_MOUSE

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Protein
Ubiquitin conjugation factor E4 B
Gene
Ube4b, Ufd2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3 By similarity.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei109 – 1102Cleavage; by caspase-3 and caspase-7 By similarityBy similarity
Sitei123 – 1242Cleavage; by caspase-6 and granzyme B By similarityBy similarity

GO - Molecular functioni

  1. enzyme binding Source: UniProtKB
  2. ubiquitin-ubiquitin ligase activity Source: InterPro
  3. unfolded protein binding Source: UniProtKB

GO - Biological processi

  1. granzyme-mediated apoptotic signaling pathway Source: UniProtKB
  2. proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
  3. protein folding Source: UniProtKB
  4. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: UniProtKB
  5. response to UV Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin conjugation factor E4 B
Alternative name(s):
Ubiquitin fusion degradation protein 2
Ufd2a
Gene namesi
Name:Ube4b
Synonyms:Ufd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1927086. Ube4b.

Subcellular locationi

Cytoplasm 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. ubiquitin ligase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11731173Ubiquitin conjugation factor E4 B
PRO_0000194994Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei31 – 311Phosphoserine By similarity
Modified residuei84 – 841Phosphoserine By similarity
Modified residuei88 – 881Phosphoserine By similarity
Modified residuei90 – 901Phosphoserine By similarity
Modified residuei101 – 1011Phosphoserine By similarity
Modified residuei103 – 1031Phosphoserine By similarity
Modified residuei674 – 6741Phosphoserine By similarity
Modified residuei1136 – 11361Phosphoserine By similarity

Post-translational modificationi

Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7 By similarity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9ES00.
PaxDbiQ9ES00.
PRIDEiQ9ES00.

PTM databases

PhosphoSiteiQ9ES00.

Expressioni

Tissue specificityi

Expressed predominantly in neuronal tissues.1 Publication

Gene expression databases

ArrayExpressiQ9ES00.
BgeeiQ9ES00.
GenevestigatoriQ9ES00.

Interactioni

Subunit structurei

Interacts with VCP.1 Publication

Protein-protein interaction databases

BioGridi211009. 8 interactions.
IntActiQ9ES00. 1 interaction.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni1101 – 11033
Turni1106 – 11083
Beta strandi1113 – 11175
Beta strandi1123 – 11253
Helixi1126 – 113510
Turni1140 – 11423
Helixi1148 – 11503
Helixi1155 – 117016

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KR4NMR-A1092-1173[»]
ProteinModelPortaliQ9ES00.
SMRiQ9ES00. Positions 1092-1173.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1098 – 117174U-box
Add
BLAST

Sequence similaritiesi

Contains 1 U-box domain.

Phylogenomic databases

eggNOGiCOG5113.
GeneTreeiENSGT00390000009300.
HOVERGENiHBG058129.
InParanoidiQ6DID4.
KOiK10597.
OMAiTENMLEP.
OrthoDBiEOG7TXKFT.
TreeFamiTF300802.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR019474. Ub_conjug_fac_E4_core.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF04564. U-box. 1 hit.
PF10408. Ufd2P_core. 1 hit.
[Graphical view]
SMARTiSM00504. Ubox. 1 hit.
[Graphical view]
PROSITEiPS51698. U_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ES00-1 [UniParc]FASTAAdd to Basket

« Hide

MEELSADEIR RRRLARLAGG QTSQPTTPLT SPQRENPPGP PIAASAPGPS     50
QSLGLNVHNM TPATSPIGAA GVAHRSQSSE GVSSLSSSPS NSLETQSQSL 100
SRSQSMDIDG VSCEKSMSQV DVDSGIENME VDENDRREKR SLSDKEPSSG 150
PEVSEEQALQ LVCKIFRVSW KDRDRDVIFL SSLSAQFKQN PKEVFSDFKD 200
LIGQILMEVL MMSTQTRDEN PFASLTATSQ PIATAARSPD RNLMLNTGSS 250
SGTSPMFCNM GSFSTSSLSS LGASGGASNW DSYSDHFTIE TCKETDMLNY 300
LIECFDRVGI EEKKAPKMCS QPAVSQLLSN IRSQCISHTA LVLQGSLTQP 350
RSLQQPSFLV PYMLCRNLPY GFIQELVRTT HQDEEVFKQI FIPILQGLAL 400
AAKECSLESD YFKYPLMALG ELCETKFGKT HPMCNLVASL PLWLPKSLSP 450
GSGRELQRLS YLGAFFSFSV FAEDDAKVVE KYFSGPAITL ENTRVVSQSL 500
QHYLELGRQE LFKILHSILL NGETREAALS YMAALVNANM KKAQMQADDR 550
LVSTDGFMLN LLWVLQQLST KIKLETVDPT YIFHPRCRIT LPNDETRINA 600
TMEDVNERLT ELYGDQPPFS EPKFPTECFF LTLHAHHLSI LPSCRRYIRR 650
LRAIRELNRT VEDLKNNESQ WKDSPLATRH REMLKRCKTQ LKKLVRCKAC 700
ADAGLLDESF LRRCLNFYGL LIQLMLRILD PAYPDVTLPL NSEVPKVFAA 750
LPEFYVEDVA EFLFFIVQYS PQVLYEPCTQ DIVMFLVVML CNQNYIRNPY 800
LVAKLVEVMF MTNPSVQPRT QKFFEMIENH PLSTKLLVPS LMKFYTDVEH 850
TGATSEFYDK FTIRYHISTI FKSLWQNIAH HGTFMEEFNS GKQFVRYINM 900
LINDTTFLLD ESLESLKRIH EVQEEMKNKE QWDQLPRDQQ QARQSQLAQD 950
ERVSRSYLAL ATETVDMFHL LTKQVQKPFL RPELGPRLAA MLNFNLQQLC 1000
GPKCRDLKVE NPEKYGFEPK KLLDQLTDIY LQLDCARFAK AIADDQRSYS 1050
KELFEEVISK MRKAGIKSTI AIEKFKLLAE KVEEIVAKNA RAEIDYSDAP 1100
DEFRDPLMDT LMTDPVRLPS GTVMDRSIIL RHLLNSPTDP FNRQMLTESM 1150
LEPVPELKEQ IQAWMREKQS SDH 1173
Length:1,173
Mass (Da):133,318
Last modified:July 27, 2011 - v3
Checksum:iFC4879E47672E983
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti298 – 2981L → P in AAG17287. 1 Publication
Sequence conflicti408 – 4081E → D in AAG17287. 1 Publication
Sequence conflicti408 – 4081E → D in BAC56586. 1 Publication
Sequence conflicti674 – 6741S → T in BAC26672. 1 Publication
Sequence conflicti697 – 6971C → Y in AAG17287. 1 Publication
Sequence conflicti753 – 7531E → K in BAC26672. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF260924 mRNA. Translation: AAG17285.1. Different termination.
AF260926 mRNA. Translation: AAG17287.1.
AF260927 Genomic DNA. Translation: AAG38492.1.
AB083274 Genomic DNA. Translation: BAC56586.1.
AK029914 mRNA. Translation: BAC26672.1.
AL606973 Genomic DNA. Translation: CAM16303.1.
BC075620 mRNA. Translation: AAH75620.1.
CCDSiCCDS18958.1.
RefSeqiNP_071305.2. NM_022022.3.
UniGeneiMm.415260.
Mm.478205.

Genome annotation databases

EnsembliENSMUST00000103212; ENSMUSP00000099501; ENSMUSG00000028960.
GeneIDi63958.
KEGGimmu:63958.
UCSCiuc012dpr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF260924 mRNA. Translation: AAG17285.1 . Different termination.
AF260926 mRNA. Translation: AAG17287.1 .
AF260927 Genomic DNA. Translation: AAG38492.1 .
AB083274 Genomic DNA. Translation: BAC56586.1 .
AK029914 mRNA. Translation: BAC26672.1 .
AL606973 Genomic DNA. Translation: CAM16303.1 .
BC075620 mRNA. Translation: AAH75620.1 .
CCDSi CCDS18958.1.
RefSeqi NP_071305.2. NM_022022.3.
UniGenei Mm.415260.
Mm.478205.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2KR4 NMR - A 1092-1173 [» ]
ProteinModelPortali Q9ES00.
SMRi Q9ES00. Positions 1092-1173.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 211009. 8 interactions.
IntActi Q9ES00. 1 interaction.

PTM databases

PhosphoSitei Q9ES00.

Proteomic databases

MaxQBi Q9ES00.
PaxDbi Q9ES00.
PRIDEi Q9ES00.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000103212 ; ENSMUSP00000099501 ; ENSMUSG00000028960 .
GeneIDi 63958.
KEGGi mmu:63958.
UCSCi uc012dpr.1. mouse.

Organism-specific databases

CTDi 10277.
MGIi MGI:1927086. Ube4b.

Phylogenomic databases

eggNOGi COG5113.
GeneTreei ENSGT00390000009300.
HOVERGENi HBG058129.
InParanoidi Q6DID4.
KOi K10597.
OMAi TENMLEP.
OrthoDBi EOG7TXKFT.
TreeFami TF300802.

Miscellaneous databases

ChiTaRSi UBE4B. mouse.
NextBioi 319825.
PROi Q9ES00.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9ES00.
Bgeei Q9ES00.
Genevestigatori Q9ES00.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR019474. Ub_conjug_fac_E4_core.
IPR003613. Ubox_domain.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
Pfami PF04564. U-box. 1 hit.
PF10408. Ufd2P_core. 1 hit.
[Graphical view ]
SMARTi SM00504. Ubox. 1 hit.
[Graphical view ]
PROSITEi PS51698. U_BOX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A Ufd2/D4Cole1e chimeric protein and overexpression of Rbp7 in the slow Wallerian degeneration (WldS) mouse."
    Conforti L., Tarlton A., Mack T.G.A., Mi W., Buckmaster E.A., Wagner D., Perry V.H., Coleman M.P.
    Proc. Natl. Acad. Sci. U.S.A. 97:11377-11382(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: C57BL/Ola.
    Tissue: Brain.
  2. "Characterization of the mouse gene for the U-box-type ubiquitin ligase UFD2a."
    Kaneko C., Hatakeyama S., Matsumoto M., Yada M., Nakayama K., Nakayama K.
    Biochem. Biophys. Res. Commun. 300:297-304(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: T-cell.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  6. Cited for: SUBCELLULAR LOCATION, ROLE IN DELAY OF WALLERIAN DEGENERATION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiUBE4B_MOUSE
AccessioniPrimary (citable) accession number: Q9ES00
Secondary accession number(s): Q6DID4, Q9EQE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2003
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In strain C57BL/Ola, a 85 kb region on chromosome 4 containing Nmnat1 and Ube4b is triplicated. Ube4b becomes linked to Nmnat1 and encodes a fusion protein located in the nucleus which is responsible for the delayed Wallerian degeneration of injured axons in C57BL/Ola.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi