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Q9ERZ8

- TRPV4_RAT

UniProt

Q9ERZ8 - TRPV4_RAT

Protein

Transient receptor potential cation channel subfamily V member 4

Gene

Trpv4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Acts as a regulator of intracellular Ca2+ in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 By similarity. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei192 – 1921ATPBy similarity
    Binding sitei201 – 2011ATPBy similarity
    Binding sitei239 – 2391ATPBy similarity
    Binding sitei248 – 2481ATPBy similarity

    GO - Molecular functioni

    1. actin binding Source: BHF-UCL
    2. actin filament binding Source: BHF-UCL
    3. alpha-tubulin binding Source: BHF-UCL
    4. ATP binding Source: UniProtKB-KW
    5. beta-tubulin binding Source: BHF-UCL
    6. calcium channel activity Source: BHF-UCL
    7. calmodulin binding Source: UniProtKB
    8. cation channel activity Source: UniProtKB
    9. microtubule binding Source: BHF-UCL
    10. osmosensor activity Source: Ensembl
    11. protein binding Source: RGD
    12. protein kinase C binding Source: BHF-UCL

    GO - Biological processi

    1. actin cytoskeleton reorganization Source: BHF-UCL
    2. actin filament organization Source: BHF-UCL
    3. calcium ion import Source: BHF-UCL
    4. cell-cell junction assembly Source: UniProtKB
    5. cellular calcium ion homeostasis Source: UniProtKB
    6. cellular hypotonic response Source: Ensembl
    7. cellular response to heat Source: UniProtKB
    8. cellular response to osmotic stress Source: UniProtKB
    9. cortical microtubule organization Source: BHF-UCL
    10. hyperosmotic salinity response Source: Ensembl
    11. hypotonic response Source: RGD
    12. microtubule polymerization Source: BHF-UCL
    13. negative regulation of neuron projection development Source: BHF-UCL
    14. osmosensory signaling pathway Source: Ensembl
    15. positive regulation of cytosolic calcium ion concentration Source: RGD
    16. positive regulation of microtubule depolymerization Source: BHF-UCL
    17. regulation of response to osmotic stress Source: Ensembl
    18. vasopressin secretion Source: Ensembl

    Keywords - Molecular functioni

    Calcium channel, Ion channel

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Calcium, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198388. TRP channels.

    Protein family/group databases

    TCDBi1.A.4.2.5. the transient receptor potential ca(2+) channel (trp-cc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transient receptor potential cation channel subfamily V member 4
    Short name:
    TrpV4
    Alternative name(s):
    Osm-9-like TRP channel 4
    Short name:
    OTRPC4
    Vanilloid receptor-related osmotically-activated channel
    Short name:
    VR-OAC
    Gene namesi
    Name:Trpv4
    Synonyms:Vroac
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 12

    Organism-specific databases

    RGDi69337. Trpv4.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Cell junctionadherens junction By similarity
    Note: Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.By similarity

    GO - Cellular componenti

    1. adherens junction Source: UniProtKB
    2. cell surface Source: RGD
    3. cilium Source: Ensembl
    4. cortical actin cytoskeleton Source: BHF-UCL
    5. cytoplasmic vesicle Source: RGD
    6. filopodium Source: BHF-UCL
    7. focal adhesion Source: BHF-UCL
    8. growth cone Source: BHF-UCL
    9. integral component of membrane Source: UniProtKB-KW
    10. lamellipodium Source: BHF-UCL
    11. plasma membrane Source: UniProtKB
    12. ruffle membrane Source: BHF-UCL

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 871871Transient receptor potential cation channel subfamily V member 4PRO_0000215349Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei824 – 8241Phosphoserine; by PKC and PKABy similarity

    Post-translational modificationi

    Phosphorylation results in enhancement of its channel function.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9ERZ8.
    PRIDEiQ9ERZ8.

    PTM databases

    PhosphoSiteiQ9ERZ8.

    Expressioni

    Tissue specificityi

    Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia.1 Publication

    Gene expression databases

    GenevestigatoriQ9ERZ8.

    Interactioni

    Subunit structurei

    Interacts with calmodulin. Interacts with MAP7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES. Interacts with CTNNB1. The TRPV4 and CTNNB1 complex can interact with CDH1 By similarity. Part of a complex containing MLC1, AQP4, HEPACAM and ATP1B1 By similarity. Interacts with PACSIN1, PACSIN2 and PACSIN3 (via SH3 domain).By similarity1 Publication

    Protein-protein interaction databases

    BioGridi249353. 3 interactions.
    DIPiDIP-59600N.
    STRINGi10116.ENSRNOP00000001586.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ERZ8.
    SMRiQ9ERZ8. Positions 149-393.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 465465CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini487 – 50822ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini530 – 55021CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini572 – 5721ExtracellularSequence Analysis
    Topological domaini594 – 61623CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini712 – 871160CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei648 – 67831Pore-formingCuratedAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei466 – 48621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei509 – 52921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei551 – 57121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei573 – 59321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei617 – 63721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei691 – 71121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati237 – 26630ANK 1Add
    BLAST
    Repeati284 – 31330ANK 2Add
    BLAST
    Repeati369 – 39830ANK 3Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni812 – 83120Interaction with calmodulinBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 3 ANK repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG278734.
    GeneTreeiENSGT00550000074425.
    HOGENOMiHOG000234630.
    HOVERGENiHBG054085.
    InParanoidiQ9ERZ8.
    KOiK04973.
    OMAiEDQTNCT.
    OrthoDBiEOG70S74P.
    PhylomeDBiQ9ERZ8.
    TreeFamiTF314711.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR005821. Ion_trans_dom.
    IPR004729. TRP_channel.
    IPR008347. TRPV1-4_channel.
    IPR008348. TRPV4_channel.
    [Graphical view]
    PANTHERiPTHR10582:SF4. PTHR10582:SF4. 1 hit.
    PfamiPF12796. Ank_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    PRINTSiPR01768. TRPVRECEPTOR.
    PR01769. VRL2RECEPTOR.
    SMARTiSM00248. ANK. 3 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    TIGRFAMsiTIGR00870. trp. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ERZ8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADPGDGPRA APGDVAEPPG DESGTSGGEA FPLSSLANLF EGEEGSSSLS    50
    PVDASRPAGP GDGRPNLRMK FQGAFRKGVP NPIDLLESTL YESSVVPGPK 100
    KAPMDSLFDY GTYRHHPSDN KRWRRKVVEK QPQSPKAPAP QPPPILKVFN 150
    RPILFDIVSR GSTADLDGLL SYLLTHKKRL TDEEFREPST GKTCLPKALL 200
    NLSNGRNDTI PVLLDIAERT GNMREFINSP FRDIYYRGQT ALHIAIERRC 250
    KHYVELLVAQ GADVHAQARG RFFQPKDEGG YFYFGELPLS LAACTNQPHI 300
    VNYLTENPHK KADMRRQDSR GNTVLHALVA IADNTRENTK FVTKMYDLLL 350
    LKCSRLFPDS NLETVLNNDG LSPLMMAAKT GKIGVFQHII RREVTDEDTR 400
    HLSRKFKDWA YGPVYSSLYD LSSLDTCGEE VSVLEILVYN SKIENRHEML 450
    AVEPINELLR DKWRKFGAVS FYINVVSYLC AMVIFTLTAY YQPLEGTPPY 500
    PYRTTVDYLR LAGEVITLLT GVLFFFTSIK DLFMKKCPGV NSLFVDGSFQ 550
    LLYFIYSVLV VVSAALYLAG IEAYLAVMVF ALVLGWMNAL YFTRGLKLTG 600
    TYSIMIQKIL FKDLFRFLLV YLLFMIGYAS ALVTLLNPCT NMKVCNEDQS 650
    NCTVPSYPAC RDSETFSAFL LDLFKLTIGM GDLEMLSSAK YPVVFILLLV 700
    TYIILTFVLL LNMLIALMGE TVGQVSKESK HIWKLQWATT ILDIERSFPV 750
    FLRKAFRSGE MVTVGKSSDG TPDRRWCFRV DEVNWSHWNQ NLGIINEDPG 800
    KSEIYQYYGF SHTMGRLRRD RWSSVVPRVV ELNKNSGTDE VVVPLDNLGN 850
    PNCDGHQQGY APKWRAEDAP L 871
    Length:871
    Mass (Da):98,010
    Last modified:March 1, 2001 - v1
    Checksum:i5D50684DA08C354B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF263521 mRNA. Translation: AAG28027.1.
    RefSeqiNP_076460.1. NM_023970.1.
    XP_006249528.1. XM_006249466.1.
    UniGeneiRn.64508.

    Genome annotation databases

    EnsembliENSRNOT00000001586; ENSRNOP00000001586; ENSRNOG00000001195.
    GeneIDi66026.
    KEGGirno:66026.
    UCSCiRGD:69337. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF263521 mRNA. Translation: AAG28027.1 .
    RefSeqi NP_076460.1. NM_023970.1.
    XP_006249528.1. XM_006249466.1.
    UniGenei Rn.64508.

    3D structure databases

    ProteinModelPortali Q9ERZ8.
    SMRi Q9ERZ8. Positions 149-393.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249353. 3 interactions.
    DIPi DIP-59600N.
    STRINGi 10116.ENSRNOP00000001586.

    Chemistry

    BindingDBi Q9ERZ8.
    ChEMBLi CHEMBL2775.
    GuidetoPHARMACOLOGYi 510.

    Protein family/group databases

    TCDBi 1.A.4.2.5. the transient receptor potential ca(2+) channel (trp-cc) family.

    PTM databases

    PhosphoSitei Q9ERZ8.

    Proteomic databases

    PaxDbi Q9ERZ8.
    PRIDEi Q9ERZ8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000001586 ; ENSRNOP00000001586 ; ENSRNOG00000001195 .
    GeneIDi 66026.
    KEGGi rno:66026.
    UCSCi RGD:69337. rat.

    Organism-specific databases

    CTDi 59341.
    RGDi 69337. Trpv4.

    Phylogenomic databases

    eggNOGi NOG278734.
    GeneTreei ENSGT00550000074425.
    HOGENOMi HOG000234630.
    HOVERGENi HBG054085.
    InParanoidi Q9ERZ8.
    KOi K04973.
    OMAi EDQTNCT.
    OrthoDBi EOG70S74P.
    PhylomeDBi Q9ERZ8.
    TreeFami TF314711.

    Enzyme and pathway databases

    Reactomei REACT_198388. TRP channels.

    Miscellaneous databases

    NextBioi 614333.
    PROi Q9ERZ8.

    Gene expression databases

    Genevestigatori Q9ERZ8.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR005821. Ion_trans_dom.
    IPR004729. TRP_channel.
    IPR008347. TRPV1-4_channel.
    IPR008348. TRPV4_channel.
    [Graphical view ]
    PANTHERi PTHR10582:SF4. PTHR10582:SF4. 1 hit.
    Pfami PF12796. Ank_2. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    PRINTSi PR01768. TRPVRECEPTOR.
    PR01769. VRL2RECEPTOR.
    SMARTi SM00248. ANK. 3 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    TIGRFAMsi TIGR00870. trp. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor."
      Liedtke W.B., Choe Y., Marti-Renom M.A., Bell A.M., Denis C.S., Sali A., Hudspeth A.J., Friedman J.M., Heller S.
      Cell 103:525-535(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Tissue: Kidney.
    2. "PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the subcellular localization of TRPV4."
      Cuajungco M.P., Grimm C., Oshima K., D'hoedt D., Nilius B., Mensenkamp A.R., Bindels R.J., Plomann M., Heller S.
      J. Biol. Chem. 281:18753-18762(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PACSIN1; PACSIN2 AND PACSIN3.

    Entry informationi

    Entry nameiTRPV4_RAT
    AccessioniPrimary (citable) accession number: Q9ERZ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3