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Q9ERZ8

- TRPV4_RAT

UniProt

Q9ERZ8 - TRPV4_RAT

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Protein

Transient receptor potential cation channel subfamily V member 4

Gene

Trpv4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Acts as a regulator of intracellular Ca2+ in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (By similarity). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei192 – 1921ATPBy similarity
Binding sitei201 – 2011ATPBy similarity
Binding sitei239 – 2391ATPBy similarity
Binding sitei248 – 2481ATPBy similarity

GO - Molecular functioni

  1. actin binding Source: BHF-UCL
  2. actin filament binding Source: BHF-UCL
  3. alpha-tubulin binding Source: BHF-UCL
  4. ATP binding Source: UniProtKB-KW
  5. beta-tubulin binding Source: BHF-UCL
  6. calcium channel activity Source: BHF-UCL
  7. calmodulin binding Source: UniProtKB
  8. cation channel activity Source: UniProtKB
  9. microtubule binding Source: BHF-UCL
  10. osmosensor activity Source: Ensembl
  11. protein kinase C binding Source: BHF-UCL

GO - Biological processi

  1. actin cytoskeleton reorganization Source: BHF-UCL
  2. actin filament organization Source: BHF-UCL
  3. calcium ion import Source: BHF-UCL
  4. cell-cell junction assembly Source: UniProtKB
  5. cellular calcium ion homeostasis Source: UniProtKB
  6. cellular hypotonic response Source: Ensembl
  7. cellular response to heat Source: UniProtKB
  8. cellular response to osmotic stress Source: UniProtKB
  9. cortical microtubule organization Source: BHF-UCL
  10. hyperosmotic salinity response Source: Ensembl
  11. hypotonic response Source: RGD
  12. microtubule polymerization Source: BHF-UCL
  13. negative regulation of neuron projection development Source: BHF-UCL
  14. osmosensory signaling pathway Source: Ensembl
  15. positive regulation of cytosolic calcium ion concentration Source: RGD
  16. positive regulation of microtubule depolymerization Source: BHF-UCL
  17. regulation of response to osmotic stress Source: Ensembl
  18. vasopressin secretion Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198388. TRP channels.

Protein family/group databases

TCDBi1.A.4.2.5. the transient receptor potential ca(2+) channel (trp-cc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily V member 4
Short name:
TrpV4
Alternative name(s):
Osm-9-like TRP channel 4
Short name:
OTRPC4
Vanilloid receptor-related osmotically-activated channel
Short name:
VR-OAC
Gene namesi
Name:Trpv4
Synonyms:Vroac
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 12

Organism-specific databases

RGDi69337. Trpv4.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity. Cell junctionadherens junction By similarity
Note: Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 465465CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei466 – 48621HelicalSequence AnalysisAdd
BLAST
Topological domaini487 – 50822ExtracellularSequence AnalysisAdd
BLAST
Transmembranei509 – 52921HelicalSequence AnalysisAdd
BLAST
Topological domaini530 – 55021CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei551 – 57121HelicalSequence AnalysisAdd
BLAST
Topological domaini572 – 5721ExtracellularSequence Analysis
Transmembranei573 – 59321HelicalSequence AnalysisAdd
BLAST
Topological domaini594 – 61623CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei617 – 63721HelicalSequence AnalysisAdd
BLAST
Intramembranei648 – 67831Pore-formingCuratedAdd
BLAST
Transmembranei691 – 71121HelicalSequence AnalysisAdd
BLAST
Topological domaini712 – 871160CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. adherens junction Source: UniProtKB
  2. cell surface Source: RGD
  3. cilium Source: Ensembl
  4. cortical actin cytoskeleton Source: BHF-UCL
  5. cytoplasmic vesicle Source: RGD
  6. filopodium Source: BHF-UCL
  7. focal adhesion Source: BHF-UCL
  8. growth cone Source: BHF-UCL
  9. integral component of membrane Source: UniProtKB-KW
  10. lamellipodium Source: BHF-UCL
  11. plasma membrane Source: UniProtKB
  12. ruffle membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 871871Transient receptor potential cation channel subfamily V member 4PRO_0000215349Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei824 – 8241Phosphoserine; by PKC and PKABy similarity

Post-translational modificationi

Phosphorylation results in enhancement of its channel function.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9ERZ8.
PRIDEiQ9ERZ8.

PTM databases

PhosphoSiteiQ9ERZ8.

Expressioni

Tissue specificityi

Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia.1 Publication

Gene expression databases

GenevestigatoriQ9ERZ8.

Interactioni

Subunit structurei

Interacts with calmodulin. Interacts with MAP7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES. Interacts with CTNNB1. The TRPV4 and CTNNB1 complex can interact with CDH1 (By similarity). Part of a complex containing MLC1, AQP4, HEPACAM and ATP1B1 (By similarity). Interacts with PACSIN1, PACSIN2 and PACSIN3 (via SH3 domain).By similarity1 Publication

Protein-protein interaction databases

BioGridi249353. 3 interactions.
DIPiDIP-59600N.
STRINGi10116.ENSRNOP00000001586.

Structurei

3D structure databases

ProteinModelPortaliQ9ERZ8.
SMRiQ9ERZ8. Positions 149-393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati237 – 26630ANK 1Add
BLAST
Repeati284 – 31330ANK 2Add
BLAST
Repeati369 – 39830ANK 3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni812 – 83120Interaction with calmodulinBy similarityAdd
BLAST

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG278734.
GeneTreeiENSGT00550000074425.
HOGENOMiHOG000234630.
HOVERGENiHBG054085.
InParanoidiQ9ERZ8.
KOiK04973.
OMAiEDQTNCT.
OrthoDBiEOG70S74P.
PhylomeDBiQ9ERZ8.
TreeFamiTF314711.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008347. TRPV1-4_channel.
IPR008348. TRPV4_channel.
[Graphical view]
PANTHERiPTHR10582:SF4. PTHR10582:SF4. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
PRINTSiPR01768. TRPVRECEPTOR.
PR01769. VRL2RECEPTOR.
SMARTiSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ERZ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADPGDGPRA APGDVAEPPG DESGTSGGEA FPLSSLANLF EGEEGSSSLS
60 70 80 90 100
PVDASRPAGP GDGRPNLRMK FQGAFRKGVP NPIDLLESTL YESSVVPGPK
110 120 130 140 150
KAPMDSLFDY GTYRHHPSDN KRWRRKVVEK QPQSPKAPAP QPPPILKVFN
160 170 180 190 200
RPILFDIVSR GSTADLDGLL SYLLTHKKRL TDEEFREPST GKTCLPKALL
210 220 230 240 250
NLSNGRNDTI PVLLDIAERT GNMREFINSP FRDIYYRGQT ALHIAIERRC
260 270 280 290 300
KHYVELLVAQ GADVHAQARG RFFQPKDEGG YFYFGELPLS LAACTNQPHI
310 320 330 340 350
VNYLTENPHK KADMRRQDSR GNTVLHALVA IADNTRENTK FVTKMYDLLL
360 370 380 390 400
LKCSRLFPDS NLETVLNNDG LSPLMMAAKT GKIGVFQHII RREVTDEDTR
410 420 430 440 450
HLSRKFKDWA YGPVYSSLYD LSSLDTCGEE VSVLEILVYN SKIENRHEML
460 470 480 490 500
AVEPINELLR DKWRKFGAVS FYINVVSYLC AMVIFTLTAY YQPLEGTPPY
510 520 530 540 550
PYRTTVDYLR LAGEVITLLT GVLFFFTSIK DLFMKKCPGV NSLFVDGSFQ
560 570 580 590 600
LLYFIYSVLV VVSAALYLAG IEAYLAVMVF ALVLGWMNAL YFTRGLKLTG
610 620 630 640 650
TYSIMIQKIL FKDLFRFLLV YLLFMIGYAS ALVTLLNPCT NMKVCNEDQS
660 670 680 690 700
NCTVPSYPAC RDSETFSAFL LDLFKLTIGM GDLEMLSSAK YPVVFILLLV
710 720 730 740 750
TYIILTFVLL LNMLIALMGE TVGQVSKESK HIWKLQWATT ILDIERSFPV
760 770 780 790 800
FLRKAFRSGE MVTVGKSSDG TPDRRWCFRV DEVNWSHWNQ NLGIINEDPG
810 820 830 840 850
KSEIYQYYGF SHTMGRLRRD RWSSVVPRVV ELNKNSGTDE VVVPLDNLGN
860 870
PNCDGHQQGY APKWRAEDAP L
Length:871
Mass (Da):98,010
Last modified:March 1, 2001 - v1
Checksum:i5D50684DA08C354B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263521 mRNA. Translation: AAG28027.1.
RefSeqiNP_076460.1. NM_023970.1.
XP_006249528.1. XM_006249466.2.
UniGeneiRn.64508.

Genome annotation databases

EnsembliENSRNOT00000001586; ENSRNOP00000001586; ENSRNOG00000001195.
GeneIDi66026.
KEGGirno:66026.
UCSCiRGD:69337. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263521 mRNA. Translation: AAG28027.1 .
RefSeqi NP_076460.1. NM_023970.1.
XP_006249528.1. XM_006249466.2.
UniGenei Rn.64508.

3D structure databases

ProteinModelPortali Q9ERZ8.
SMRi Q9ERZ8. Positions 149-393.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249353. 3 interactions.
DIPi DIP-59600N.
STRINGi 10116.ENSRNOP00000001586.

Chemistry

BindingDBi Q9ERZ8.
ChEMBLi CHEMBL2096684.
GuidetoPHARMACOLOGYi 510.

Protein family/group databases

TCDBi 1.A.4.2.5. the transient receptor potential ca(2+) channel (trp-cc) family.

PTM databases

PhosphoSitei Q9ERZ8.

Proteomic databases

PaxDbi Q9ERZ8.
PRIDEi Q9ERZ8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000001586 ; ENSRNOP00000001586 ; ENSRNOG00000001195 .
GeneIDi 66026.
KEGGi rno:66026.
UCSCi RGD:69337. rat.

Organism-specific databases

CTDi 59341.
RGDi 69337. Trpv4.

Phylogenomic databases

eggNOGi NOG278734.
GeneTreei ENSGT00550000074425.
HOGENOMi HOG000234630.
HOVERGENi HBG054085.
InParanoidi Q9ERZ8.
KOi K04973.
OMAi EDQTNCT.
OrthoDBi EOG70S74P.
PhylomeDBi Q9ERZ8.
TreeFami TF314711.

Enzyme and pathway databases

Reactomei REACT_198388. TRP channels.

Miscellaneous databases

NextBioi 614333.
PROi Q9ERZ8.

Gene expression databases

Genevestigatori Q9ERZ8.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR008347. TRPV1-4_channel.
IPR008348. TRPV4_channel.
[Graphical view ]
PANTHERi PTHR10582:SF4. PTHR10582:SF4. 1 hit.
Pfami PF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view ]
PRINTSi PR01768. TRPVRECEPTOR.
PR01769. VRL2RECEPTOR.
SMARTi SM00248. ANK. 3 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
TIGRFAMsi TIGR00870. trp. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor."
    Liedtke W.B., Choe Y., Marti-Renom M.A., Bell A.M., Denis C.S., Sali A., Hudspeth A.J., Friedman J.M., Heller S.
    Cell 103:525-535(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Kidney.
  2. "PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the subcellular localization of TRPV4."
    Cuajungco M.P., Grimm C., Oshima K., D'hoedt D., Nilius B., Mensenkamp A.R., Bindels R.J., Plomann M., Heller S.
    J. Biol. Chem. 281:18753-18762(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PACSIN1; PACSIN2 AND PACSIN3.

Entry informationi

Entry nameiTRPV4_RAT
AccessioniPrimary (citable) accession number: Q9ERZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3