Reviewed, UniProtKB/Swiss-Prot Q9ERZ8 (TRPV4_RAT)

Names and origin

Protein names

Recommended name:
    Transient receptor potential cation channel subfamily V member 4
      Short name=TrpV4
Alternative name(s):
    Osm-9-like TRP channel 4
      Short name=OTRPC4
    Vanilloid receptor-related osmotically-activated channel
      Short name=VR-OAC

Gene names

Name:	Trpv4
Synonyms:	Vroac

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	871 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca2+ potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism By similarity. Ref.1
Subunit structure	Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Tissue specificity	Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia. Ref.1
Sequence similarities	Belongs to the transient receptor family. TrpV subfamily. Contains 3 ANK repeats.

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane
Domain	ANK repeat Repeat Transmembrane
Ligand	Calcium Calmodulin-binding
Molecular function	Calcium channel Ionic channel
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	calcium ion transport Inferred from electronic annotation. Source: UniProtKB-KW hypotonic response Ref.1 Inferred from direct assay. Source: RGD transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	calcium channel activity Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 871	871	Transient receptor potential cation channel subfamily V member 4		PRO_0000215349
Regions
	Topological domain	1 – 465	465	Cytoplasmic Potential
	Transmembrane	466 – 486	21	Potential
	Topological domain	487 – 508	22	Extracellular Potential
	Transmembrane	509 – 529	21	Potential
	Topological domain	530 – 550	21	Cytoplasmic Potential
	Transmembrane	551 – 571	21	Potential
	Topological domain	572	1	Extracellular Potential
	Transmembrane	573 – 593	21	Potential
	Topological domain	594 – 616	23	Cytoplasmic Potential
	Transmembrane	617 – 637	21	Potential
	Topological domain	638 – 690	53	Pore forming Probable
	Transmembrane	691 – 711	21	Potential
	Topological domain	712 – 871	160	Cytoplasmic Potential
	Repeat	237 – 266	30	ANK 1
	Repeat	284 – 313	30	ANK 2
	Repeat	369 – 398	30	ANK 3
	Region	812 – 831	20	Interaction with calmodulin By similarity
Amino acid modifications
	Modified residue	253	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9ERZ8-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 5D50684DA08C354B	FASTA	871	98,010
	10 20 30 40 50 60 MADPGDGPRA APGDVAEPPG DESGTSGGEA FPLSSLANLF EGEEGSSSLS PVDASRPAGP 70 80 90 100 110 120 GDGRPNLRMK FQGAFRKGVP NPIDLLESTL YESSVVPGPK KAPMDSLFDY GTYRHHPSDN 130 140 150 160 170 180 KRWRRKVVEK QPQSPKAPAP QPPPILKVFN RPILFDIVSR GSTADLDGLL SYLLTHKKRL 190 200 210 220 230 240 TDEEFREPST GKTCLPKALL NLSNGRNDTI PVLLDIAERT GNMREFINSP FRDIYYRGQT 250 260 270 280 290 300 ALHIAIERRC KHYVELLVAQ GADVHAQARG RFFQPKDEGG YFYFGELPLS LAACTNQPHI 310 320 330 340 350 360 VNYLTENPHK KADMRRQDSR GNTVLHALVA IADNTRENTK FVTKMYDLLL LKCSRLFPDS 370 380 390 400 410 420 NLETVLNNDG LSPLMMAAKT GKIGVFQHII RREVTDEDTR HLSRKFKDWA YGPVYSSLYD 430 440 450 460 470 480 LSSLDTCGEE VSVLEILVYN SKIENRHEML AVEPINELLR DKWRKFGAVS FYINVVSYLC 490 500 510 520 530 540 AMVIFTLTAY YQPLEGTPPY PYRTTVDYLR LAGEVITLLT GVLFFFTSIK DLFMKKCPGV 550 560 570 580 590 600 NSLFVDGSFQ LLYFIYSVLV VVSAALYLAG IEAYLAVMVF ALVLGWMNAL YFTRGLKLTG 610 620 630 640 650 660 TYSIMIQKIL FKDLFRFLLV YLLFMIGYAS ALVTLLNPCT NMKVCNEDQS NCTVPSYPAC 670 680 690 700 710 720 RDSETFSAFL LDLFKLTIGM GDLEMLSSAK YPVVFILLLV TYIILTFVLL LNMLIALMGE 730 740 750 760 770 780 TVGQVSKESK HIWKLQWATT ILDIERSFPV FLRKAFRSGE MVTVGKSSDG TPDRRWCFRV 790 800 810 820 830 840 DEVNWSHWNQ NLGIINEDPG KSEIYQYYGF SHTMGRLRRD RWSSVVPRVV ELNKNSGTDE 850 860 870 VVVPLDNLGN PNCDGHQQGY APKWRAEDAP L

References

[1]	"Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor." Liedtke W.B., Choe Y., Marti-Renom M.A., Bell A.M., Denis C.S., Sali A., Hudspeth A.J., Friedman J.M., Heller S. Cell 103:525-535(2000) [PubMed: 11081638] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF263521 mRNA. Translation: AAG28027.1.
IPI	IPI00191593.
RefSeq	NP_076460.1.
UniGene	Rn.64508
3D structure databases
SMR	Q9ERZ8. Positions 149-393.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ERZ8.
Protein family/group databases
TCDB	1.A.4.2.5. transient receptor potential Ca2+ channel (TRP-CC) family.
Genome annotation databases
Ensembl	ENSRNOT00000001586; ENSRNOP00000001586; ENSRNOG00000001195; Rattus norvegicus. [Genome view]
GeneID	66026.
KEGG	rno:66026.
UCSC	NM_023970. rat.
Organism-specific databases
CTD	66026.
RGD	69337. Trpv4.
Phylogenomic databases
eggNOG	roNOG14417.
HOVERGEN	Q9ERZ8.
InParanoid	Q9ERZ8.
OMA	ESTLYES.
OrthoDB	EOG9SR0FX.
PhylomeDB	Q9ERZ8.
Gene expression databases
ArrayExpress	Q9ERZ8.
Genevestigator	Q9ERZ8.
GermOnline	ENSRNOG00000001195. Rattus norvegicus.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR005821. Ion_trans. IPR004729. TRP_channel. IPR008347. Vanilpoid_rcpt. IPR008348. Vanilpoid_rcpt_2. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
Pfam	PF00023. Ank. 1 hit. PF00520. Ion_trans. 1 hit. [Graphical view]
PRINTS	PR01768. TRPVRECEPTOR. PR01769. VRL2RECEPTOR.
SMART	SM00248. ANK. 3 hits. [Graphical view]
TIGRFAMs	TIGR00870. trp. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	614333.

Entry information

Entry name

TRPV4_RAT

Accession

Primary (citable) accession number: Q9ERZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)