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Q9ERZ4 (ACM2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Muscarinic acetylcholine receptor M2
Gene names
Name:Chrm2
Synonyms:Chrm-2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.

Subunit structure

Interacts with ARRB1 and ARRB2. Interacts with GNB2L1/RACK1; the interaction regulates CHRM2 internalization By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466Muscarinic acetylcholine receptor M2
PRO_0000069022

Regions

Topological domain1 – 2222Extracellular By similarity
Transmembrane23 – 4523Helical; Name=1; By similarity
Topological domain46 – 5914Cytoplasmic By similarity
Transmembrane60 – 8021Helical; Name=2; By similarity
Topological domain81 – 9717Extracellular By similarity
Transmembrane98 – 11922Helical; Name=3; By similarity
Topological domain120 – 13920Cytoplasmic By similarity
Transmembrane140 – 16223Helical; Name=4; By similarity
Topological domain163 – 18422Extracellular By similarity
Transmembrane185 – 20723Helical; Name=5; By similarity
Topological domain208 – 388181Cytoplasmic By similarity
Transmembrane389 – 40921Helical; Name=6; By similarity
Topological domain410 – 42314Extracellular By similarity
Transmembrane424 – 44320Helical; Name=7; By similarity
Topological domain444 – 46623Cytoplasmic By similarity

Amino acid modifications

Modified residue4461Phosphothreonine Potential
Modified residue4501Phosphothreonine Potential
Modified residue4651Phosphothreonine Potential
Glycosylation21N-linked (GlcNAc...) Potential
Glycosylation31N-linked (GlcNAc...) Potential
Glycosylation61N-linked (GlcNAc...) Potential
Disulfide bond96 ↔ 176 By similarity

Experimental info

Sequence conflict2661P → L in AAG14343. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9ERZ4 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 93F90B639CB45AE3

FASTA46651,499
        10         20         30         40         50         60 
MNNSTNSSNN GLAITSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV NRHLQTVNNY 

        70         80         90        100        110        120 
FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL ALDYVVSNAS VMNLLIISFD 

       130        140        150        160        170        180 
RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL SFILWAPAIL FWQFIVGVRT VEDGECYIQF 

       190        200        210        220        230        240 
FSNAAVTFGT AIAAFYLPVI IMTVLYWHIS RASKSRIKKE KKEPVANQDP VSPSLVQGRI 

       250        260        270        280        290        300 
VKPNNNNMPG GDGGLEHNKI QNGKAPRDGG TENCVQGEEK ESSNDSTSVS AVASNMRDDE 

       310        320        330        340        350        360 
ITQDENTVST SLGHSKDDNS RQTCIKIVTK TQKGDACTPT STTVELVGSS GQNGDEKQNI 

       370        380        390        400        410        420 
VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW APYNVMVLIN TFCAPCIPNT 

       430        440        450        460 
VWTIGYWLCY INSTINPACY ALCNATFKKT FKHLLMCHYK NIGATR 

« Hide

References

« Hide 'large scale' references
[1]"Isolation, sequence and functional expression of mouse muscarinic acetylcholine receptor genes."
Gomeza J., Wess J.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF264049 Genomic DNA. Translation: AAG14343.1.
AC113246 Genomic DNA. No translation available.
AC159505 Genomic DNA. No translation available.
RefSeqNP_987076.2. NM_203491.3.
XP_006506199.1. XM_006506136.1.
XP_006506200.1. XM_006506137.1.
UniGeneMm.448632.

3D structure databases

ProteinModelPortalQ9ERZ4.
SMRQ9ERZ4. Positions 20-456.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ9ERZ4.
ChEMBLCHEMBL2097162.
GuidetoPHARMACOLOGY14.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9ERZ4.

Proteomic databases

PaxDbQ9ERZ4.
PRIDEQ9ERZ4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000172278; ENSMUSP00000130874; ENSMUSG00000045613.
GeneID243764.
KEGGmmu:243764.
UCSCuc009biy.2. mouse.

Organism-specific databases

CTD1129.
MGIMGI:88397. Chrm2.

Phylogenomic databases

eggNOGNOG250863.
GeneTreeENSGT00690000101881.
HOGENOMHOG000231484.
HOVERGENHBG105720.
InParanoidQ9ERZ4.
KOK04130.
OMADGGLEHN.
OrthoDBEOG7V49Z7.
PhylomeDBQ9ERZ4.
TreeFamTF320495.

Gene expression databases

BgeeQ9ERZ4.
CleanExMM_CHRM2.
GenevestigatorQ9ERZ4.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_Ach_M2_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERPTHR24249:SF57. PTHR24249:SF57. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio385914.
PROQ9ERZ4.
SOURCESearch...

Entry information

Entry nameACM2_MOUSE
AccessionPrimary (citable) accession number: Q9ERZ4
Secondary accession number(s): E9Q9P0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries