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Protein

Muscarinic acetylcholine receptor M2

Gene

Chrm2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-390648. Muscarinic acetylcholine receptors.
R-MMU-418594. G alpha (i) signalling events.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M2
Gene namesi
Name:Chrm2
Synonyms:Chrm-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88397. Chrm2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22ExtracellularBy similarityAdd BLAST22
Transmembranei23 – 45Helical; Name=1By similarityAdd BLAST23
Topological domaini46 – 59CytoplasmicBy similarityAdd BLAST14
Transmembranei60 – 80Helical; Name=2By similarityAdd BLAST21
Topological domaini81 – 97ExtracellularBy similarityAdd BLAST17
Transmembranei98 – 119Helical; Name=3By similarityAdd BLAST22
Topological domaini120 – 139CytoplasmicBy similarityAdd BLAST20
Transmembranei140 – 162Helical; Name=4By similarityAdd BLAST23
Topological domaini163 – 184ExtracellularBy similarityAdd BLAST22
Transmembranei185 – 209Helical; Name=5By similarityAdd BLAST25
Topological domaini210 – 387CytoplasmicBy similarityAdd BLAST178
Transmembranei388 – 410Helical; Name=6By similarityAdd BLAST23
Topological domaini411 – 418ExtracellularBy similarity8
Transmembranei419 – 442Helical; Name=7By similarityAdd BLAST24
Topological domaini443 – 466CytoplasmicBy similarityAdd BLAST24

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3197.
GuidetoPHARMACOLOGYi14.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690221 – 466Muscarinic acetylcholine receptor M2Add BLAST466

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi2N-linked (GlcNAc...)Sequence analysis1
Glycosylationi3N-linked (GlcNAc...)Sequence analysis1
Glycosylationi6N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi96 ↔ 176PROSITE-ProRule annotation
Modified residuei232PhosphoserineCombined sources1
Disulfide bondi413 ↔ 416PROSITE-ProRule annotation
Modified residuei446PhosphothreonineSequence analysis1
Modified residuei450PhosphothreonineSequence analysis1
Modified residuei465PhosphothreonineSequence analysis1

Post-translational modificationi

Phosphorylated in response to agonist treatment.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9ERZ4.
PRIDEiQ9ERZ4.

PTM databases

iPTMnetiQ9ERZ4.
PhosphoSitePlusiQ9ERZ4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000045613.
CleanExiMM_CHRM2.

Interactioni

Subunit structurei

Interacts with ARRB1 and ARRB2. Interacts with RACK1; the interaction regulates CHRM2 internalization (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000130874.

Chemistry databases

BindingDBiQ9ERZ4.

Structurei

3D structure databases

ProteinModelPortaliQ9ERZ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 107Agonist bindingBy similarity5
Regioni403 – 404Agonist bindingBy similarity2
Regioni426 – 430Agonist bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi120 – 122Important for signaling3
Motifi436 – 440Important for signaling5

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiQ9ERZ4.
KOiK04130.
OMAiQNGKGPS.
OrthoDBiEOG091G06VI.
PhylomeDBiQ9ERZ4.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_Ach_M2_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF57. PTHR24249:SF57. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ERZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSTNSSNN GLAITSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV
60 70 80 90 100
NRHLQTVNNY FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL
110 120 130 140 150
ALDYVVSNAS VMNLLIISFD RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL
160 170 180 190 200
SFILWAPAIL FWQFIVGVRT VEDGECYIQF FSNAAVTFGT AIAAFYLPVI
210 220 230 240 250
IMTVLYWHIS RASKSRIKKE KKEPVANQDP VSPSLVQGRI VKPNNNNMPG
260 270 280 290 300
GDGGLEHNKI QNGKAPRDGG TENCVQGEEK ESSNDSTSVS AVASNMRDDE
310 320 330 340 350
ITQDENTVST SLGHSKDDNS RQTCIKIVTK TQKGDACTPT STTVELVGSS
360 370 380 390 400
GQNGDEKQNI VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW
410 420 430 440 450
APYNVMVLIN TFCAPCIPNT VWTIGYWLCY INSTINPACY ALCNATFKKT
460
FKHLLMCHYK NIGATR
Length:466
Mass (Da):51,499
Last modified:July 27, 2011 - v2
Checksum:i93F90B639CB45AE3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti266P → L in AAG14343 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264049 Genomic DNA. Translation: AAG14343.1.
AC113246 Genomic DNA. No translation available.
AC159505 Genomic DNA. No translation available.
CCDSiCCDS20004.1.
RefSeqiNP_987076.2. NM_203491.3.
XP_006506199.1. XM_006506136.2.
UniGeneiMm.448632.

Genome annotation databases

EnsembliENSMUST00000172278; ENSMUSP00000130874; ENSMUSG00000045613.
GeneIDi243764.
KEGGimmu:243764.
UCSCiuc009biy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264049 Genomic DNA. Translation: AAG14343.1.
AC113246 Genomic DNA. No translation available.
AC159505 Genomic DNA. No translation available.
CCDSiCCDS20004.1.
RefSeqiNP_987076.2. NM_203491.3.
XP_006506199.1. XM_006506136.2.
UniGeneiMm.448632.

3D structure databases

ProteinModelPortaliQ9ERZ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000130874.

Chemistry databases

BindingDBiQ9ERZ4.
ChEMBLiCHEMBL3197.
GuidetoPHARMACOLOGYi14.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ9ERZ4.
PhosphoSitePlusiQ9ERZ4.

Proteomic databases

PaxDbiQ9ERZ4.
PRIDEiQ9ERZ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000172278; ENSMUSP00000130874; ENSMUSG00000045613.
GeneIDi243764.
KEGGimmu:243764.
UCSCiuc009biy.2. mouse.

Organism-specific databases

CTDi1129.
MGIiMGI:88397. Chrm2.

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiQ9ERZ4.
KOiK04130.
OMAiQNGKGPS.
OrthoDBiEOG091G06VI.
PhylomeDBiQ9ERZ4.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiR-MMU-390648. Muscarinic acetylcholine receptors.
R-MMU-418594. G alpha (i) signalling events.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

PROiQ9ERZ4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045613.
CleanExiMM_CHRM2.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_Ach_M2_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF57. PTHR24249:SF57. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACM2_MOUSE
AccessioniPrimary (citable) accession number: Q9ERZ4
Secondary accession number(s): E9Q9P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.