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Q9ERZ4

- ACM2_MOUSE

UniProt

Q9ERZ4 - ACM2_MOUSE

Protein

Muscarinic acetylcholine receptor M2

Gene

Chrm2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol By similarity.By similarity

    GO - Molecular functioni

    1. G-protein coupled acetylcholine receptor activity Source: UniProtKB

    GO - Biological processi

    1. G-protein coupled acetylcholine receptor signaling pathway Source: UniProtKB
    2. positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: UniProtKB
    3. regulation of heart contraction Source: InterPro
    4. response to virus Source: Ensembl

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Muscarinic acetylcholine receptor M2
    Gene namesi
    Name:Chrm2
    Synonyms:Chrm-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:88397. Chrm2.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Cell junctionsynapsepostsynaptic cell membrane By similarity; Multi-pass membrane protein By similarity
    Note: Phosphorylation in response to agonist binding promotes receptor internalization.By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. integral component of plasma membrane Source: UniProtKB
    3. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 466466Muscarinic acetylcholine receptor M2PRO_0000069022Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi2 – 21N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi3 – 31N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi6 – 61N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi96 ↔ 176PROSITE-ProRule annotation
    Disulfide bondi413 ↔ 416PROSITE-ProRule annotation
    Modified residuei446 – 4461PhosphothreonineSequence Analysis
    Modified residuei450 – 4501PhosphothreonineSequence Analysis
    Modified residuei465 – 4651PhosphothreonineSequence Analysis

    Post-translational modificationi

    Phosphorylated in response to agonist treatment.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ9ERZ4.
    PRIDEiQ9ERZ4.

    PTM databases

    PhosphoSiteiQ9ERZ4.

    Expressioni

    Gene expression databases

    BgeeiQ9ERZ4.
    CleanExiMM_CHRM2.
    GenevestigatoriQ9ERZ4.

    Interactioni

    Subunit structurei

    Interacts with ARRB1 and ARRB2. Interacts with GNB2L1/RACK1; the interaction regulates CHRM2 internalization By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ERZ4.
    SMRiQ9ERZ4. Positions 20-217, 377-456.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2222ExtracellularBy similarityAdd
    BLAST
    Topological domaini46 – 5914CytoplasmicBy similarityAdd
    BLAST
    Topological domaini81 – 9717ExtracellularBy similarityAdd
    BLAST
    Topological domaini120 – 13920CytoplasmicBy similarityAdd
    BLAST
    Topological domaini163 – 18422ExtracellularBy similarityAdd
    BLAST
    Topological domaini210 – 387178CytoplasmicBy similarityAdd
    BLAST
    Topological domaini411 – 4188ExtracellularBy similarity
    Topological domaini443 – 46624CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei23 – 4523Helical; Name=1By similarityAdd
    BLAST
    Transmembranei60 – 8021Helical; Name=2By similarityAdd
    BLAST
    Transmembranei98 – 11922Helical; Name=3By similarityAdd
    BLAST
    Transmembranei140 – 16223Helical; Name=4By similarityAdd
    BLAST
    Transmembranei185 – 20925Helical; Name=5By similarityAdd
    BLAST
    Transmembranei388 – 41023Helical; Name=6By similarityAdd
    BLAST
    Transmembranei419 – 44224Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni103 – 1075Agonist bindingBy similarity
    Regioni403 – 4042Agonist bindingBy similarity
    Regioni426 – 4305Agonist bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi120 – 1223Important for signaling
    Motifi436 – 4405Important for signaling

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG250863.
    GeneTreeiENSGT00690000101881.
    HOGENOMiHOG000231484.
    HOVERGENiHBG105720.
    InParanoidiQ9ERZ4.
    KOiK04130.
    OMAiDGGLEHN.
    OrthoDBiEOG7V49Z7.
    PhylomeDBiQ9ERZ4.
    TreeFamiTF320495.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001065. Musac_Ach_M2_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view]
    PANTHERiPTHR24249:SF57. PTHR24249:SF57. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00539. MUSCRINICM2R.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ERZ4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNSTNSSNN GLAITSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV    50
    NRHLQTVNNY FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL 100
    ALDYVVSNAS VMNLLIISFD RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL 150
    SFILWAPAIL FWQFIVGVRT VEDGECYIQF FSNAAVTFGT AIAAFYLPVI 200
    IMTVLYWHIS RASKSRIKKE KKEPVANQDP VSPSLVQGRI VKPNNNNMPG 250
    GDGGLEHNKI QNGKAPRDGG TENCVQGEEK ESSNDSTSVS AVASNMRDDE 300
    ITQDENTVST SLGHSKDDNS RQTCIKIVTK TQKGDACTPT STTVELVGSS 350
    GQNGDEKQNI VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW 400
    APYNVMVLIN TFCAPCIPNT VWTIGYWLCY INSTINPACY ALCNATFKKT 450
    FKHLLMCHYK NIGATR 466
    Length:466
    Mass (Da):51,499
    Last modified:July 27, 2011 - v2
    Checksum:i93F90B639CB45AE3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti266 – 2661P → L in AAG14343. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF264049 Genomic DNA. Translation: AAG14343.1.
    AC113246 Genomic DNA. No translation available.
    AC159505 Genomic DNA. No translation available.
    CCDSiCCDS20004.1.
    RefSeqiNP_987076.2. NM_203491.3.
    XP_006506199.1. XM_006506136.1.
    XP_006506200.1. XM_006506137.1.
    UniGeneiMm.448632.

    Genome annotation databases

    EnsembliENSMUST00000172278; ENSMUSP00000130874; ENSMUSG00000045613.
    GeneIDi243764.
    KEGGimmu:243764.
    UCSCiuc009biy.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF264049 Genomic DNA. Translation: AAG14343.1 .
    AC113246 Genomic DNA. No translation available.
    AC159505 Genomic DNA. No translation available.
    CCDSi CCDS20004.1.
    RefSeqi NP_987076.2. NM_203491.3.
    XP_006506199.1. XM_006506136.1.
    XP_006506200.1. XM_006506137.1.
    UniGenei Mm.448632.

    3D structure databases

    ProteinModelPortali Q9ERZ4.
    SMRi Q9ERZ4. Positions 20-217, 377-456.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q9ERZ4.
    ChEMBLi CHEMBL2097162.
    GuidetoPHARMACOLOGYi 14.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q9ERZ4.

    Proteomic databases

    PaxDbi Q9ERZ4.
    PRIDEi Q9ERZ4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000172278 ; ENSMUSP00000130874 ; ENSMUSG00000045613 .
    GeneIDi 243764.
    KEGGi mmu:243764.
    UCSCi uc009biy.2. mouse.

    Organism-specific databases

    CTDi 1129.
    MGIi MGI:88397. Chrm2.

    Phylogenomic databases

    eggNOGi NOG250863.
    GeneTreei ENSGT00690000101881.
    HOGENOMi HOG000231484.
    HOVERGENi HBG105720.
    InParanoidi Q9ERZ4.
    KOi K04130.
    OMAi DGGLEHN.
    OrthoDBi EOG7V49Z7.
    PhylomeDBi Q9ERZ4.
    TreeFami TF320495.

    Miscellaneous databases

    NextBioi 385914.
    PROi Q9ERZ4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9ERZ4.
    CleanExi MM_CHRM2.
    Genevestigatori Q9ERZ4.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001065. Musac_Ach_M2_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view ]
    PANTHERi PTHR24249:SF57. PTHR24249:SF57. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00539. MUSCRINICM2R.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, sequence and functional expression of mouse muscarinic acetylcholine receptor genes."
      Gomeza J., Wess J.
      Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiACM2_MOUSE
    AccessioniPrimary (citable) accession number: Q9ERZ4
    Secondary accession number(s): E9Q9P0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3