Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Muscarinic acetylcholine receptor M2

Gene

Chrm2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_279397. Muscarinic acetylcholine receptors.
REACT_331048. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M2
Gene namesi
Name:Chrm2
Synonyms:Chrm-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:88397. Chrm2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222ExtracellularBy similarityAdd
BLAST
Transmembranei23 – 4523Helical; Name=1By similarityAdd
BLAST
Topological domaini46 – 5914CytoplasmicBy similarityAdd
BLAST
Transmembranei60 – 8021Helical; Name=2By similarityAdd
BLAST
Topological domaini81 – 9717ExtracellularBy similarityAdd
BLAST
Transmembranei98 – 11922Helical; Name=3By similarityAdd
BLAST
Topological domaini120 – 13920CytoplasmicBy similarityAdd
BLAST
Transmembranei140 – 16223Helical; Name=4By similarityAdd
BLAST
Topological domaini163 – 18422ExtracellularBy similarityAdd
BLAST
Transmembranei185 – 20925Helical; Name=5By similarityAdd
BLAST
Topological domaini210 – 387178CytoplasmicBy similarityAdd
BLAST
Transmembranei388 – 41023Helical; Name=6By similarityAdd
BLAST
Topological domaini411 – 4188ExtracellularBy similarity
Transmembranei419 – 44224Helical; Name=7By similarityAdd
BLAST
Topological domaini443 – 46624CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Muscarinic acetylcholine receptor M2PRO_0000069022Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)Sequence Analysis
Glycosylationi3 – 31N-linked (GlcNAc...)Sequence Analysis
Glycosylationi6 – 61N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi96 ↔ 176PROSITE-ProRule annotation
Disulfide bondi413 ↔ 416PROSITE-ProRule annotation
Modified residuei446 – 4461PhosphothreonineSequence Analysis
Modified residuei450 – 4501PhosphothreonineSequence Analysis
Modified residuei465 – 4651PhosphothreonineSequence Analysis

Post-translational modificationi

Phosphorylated in response to agonist treatment.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9ERZ4.
PRIDEiQ9ERZ4.

PTM databases

PhosphoSiteiQ9ERZ4.

Expressioni

Gene expression databases

BgeeiQ9ERZ4.
CleanExiMM_CHRM2.

Interactioni

Subunit structurei

Interacts with ARRB1 and ARRB2. Interacts with GNB2L1/RACK1; the interaction regulates CHRM2 internalization (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000130874.

Structurei

3D structure databases

ProteinModelPortaliQ9ERZ4.
SMRiQ9ERZ4. Positions 19-456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni103 – 1075Agonist bindingBy similarity
Regioni403 – 4042Agonist bindingBy similarity
Regioni426 – 4305Agonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi120 – 1223Important for signaling
Motifi436 – 4405Important for signaling

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiQ9ERZ4.
KOiK04130.
OMAiQYKNIRS.
OrthoDBiEOG7V49Z7.
PhylomeDBiQ9ERZ4.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_Ach_M2_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF57. PTHR24249:SF57. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ERZ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSTNSSNN GLAITSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV
60 70 80 90 100
NRHLQTVNNY FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL
110 120 130 140 150
ALDYVVSNAS VMNLLIISFD RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL
160 170 180 190 200
SFILWAPAIL FWQFIVGVRT VEDGECYIQF FSNAAVTFGT AIAAFYLPVI
210 220 230 240 250
IMTVLYWHIS RASKSRIKKE KKEPVANQDP VSPSLVQGRI VKPNNNNMPG
260 270 280 290 300
GDGGLEHNKI QNGKAPRDGG TENCVQGEEK ESSNDSTSVS AVASNMRDDE
310 320 330 340 350
ITQDENTVST SLGHSKDDNS RQTCIKIVTK TQKGDACTPT STTVELVGSS
360 370 380 390 400
GQNGDEKQNI VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW
410 420 430 440 450
APYNVMVLIN TFCAPCIPNT VWTIGYWLCY INSTINPACY ALCNATFKKT
460
FKHLLMCHYK NIGATR
Length:466
Mass (Da):51,499
Last modified:July 27, 2011 - v2
Checksum:i93F90B639CB45AE3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti266 – 2661P → L in AAG14343 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264049 Genomic DNA. Translation: AAG14343.1.
AC113246 Genomic DNA. No translation available.
AC159505 Genomic DNA. No translation available.
CCDSiCCDS20004.1.
RefSeqiNP_987076.2. NM_203491.3.
XP_006506199.1. XM_006506136.2.
XP_006506200.1. XM_006506137.1.
UniGeneiMm.448632.

Genome annotation databases

EnsembliENSMUST00000172278; ENSMUSP00000130874; ENSMUSG00000045613.
GeneIDi243764.
KEGGimmu:243764.
UCSCiuc009biy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264049 Genomic DNA. Translation: AAG14343.1.
AC113246 Genomic DNA. No translation available.
AC159505 Genomic DNA. No translation available.
CCDSiCCDS20004.1.
RefSeqiNP_987076.2. NM_203491.3.
XP_006506199.1. XM_006506136.2.
XP_006506200.1. XM_006506137.1.
UniGeneiMm.448632.

3D structure databases

ProteinModelPortaliQ9ERZ4.
SMRiQ9ERZ4. Positions 19-456.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000130874.

Chemistry

BindingDBiQ9ERZ4.
ChEMBLiCHEMBL2097162.
GuidetoPHARMACOLOGYi14.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9ERZ4.

Proteomic databases

PaxDbiQ9ERZ4.
PRIDEiQ9ERZ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000172278; ENSMUSP00000130874; ENSMUSG00000045613.
GeneIDi243764.
KEGGimmu:243764.
UCSCiuc009biy.2. mouse.

Organism-specific databases

CTDi1129.
MGIiMGI:88397. Chrm2.

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiQ9ERZ4.
KOiK04130.
OMAiQYKNIRS.
OrthoDBiEOG7V49Z7.
PhylomeDBiQ9ERZ4.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiREACT_279397. Muscarinic acetylcholine receptors.
REACT_331048. G alpha (i) signalling events.

Miscellaneous databases

NextBioi385914.
PROiQ9ERZ4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9ERZ4.
CleanExiMM_CHRM2.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_Ach_M2_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF57. PTHR24249:SF57. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, sequence and functional expression of mouse muscarinic acetylcholine receptor genes."
    Gomeza J., Wess J.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiACM2_MOUSE
AccessioniPrimary (citable) accession number: Q9ERZ4
Secondary accession number(s): E9Q9P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.