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Q9ERZ4

- ACM2_MOUSE

UniProt

Q9ERZ4 - ACM2_MOUSE

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Protein

Muscarinic acetylcholine receptor M2

Gene

Chrm2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol (By similarity).By similarity

GO - Molecular functioni

  1. G-protein coupled acetylcholine receptor activity Source: UniProtKB

GO - Biological processi

  1. G-protein coupled acetylcholine receptor signaling pathway Source: UniProtKB
  2. positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: UniProtKB
  3. regulation of heart contraction Source: InterPro
  4. response to virus Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_240390. Muscarinic acetylcholine receptors.
REACT_250376. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M2
Gene namesi
Name:Chrm2
Synonyms:Chrm-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:88397. Chrm2.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity. Cell junctionsynapsepostsynaptic cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Phosphorylation in response to agonist binding promotes receptor internalization.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222ExtracellularBy similarityAdd
BLAST
Transmembranei23 – 4523Helical; Name=1By similarityAdd
BLAST
Topological domaini46 – 5914CytoplasmicBy similarityAdd
BLAST
Transmembranei60 – 8021Helical; Name=2By similarityAdd
BLAST
Topological domaini81 – 9717ExtracellularBy similarityAdd
BLAST
Transmembranei98 – 11922Helical; Name=3By similarityAdd
BLAST
Topological domaini120 – 13920CytoplasmicBy similarityAdd
BLAST
Transmembranei140 – 16223Helical; Name=4By similarityAdd
BLAST
Topological domaini163 – 18422ExtracellularBy similarityAdd
BLAST
Transmembranei185 – 20925Helical; Name=5By similarityAdd
BLAST
Topological domaini210 – 387178CytoplasmicBy similarityAdd
BLAST
Transmembranei388 – 41023Helical; Name=6By similarityAdd
BLAST
Topological domaini411 – 4188ExtracellularBy similarity
Transmembranei419 – 44224Helical; Name=7By similarityAdd
BLAST
Topological domaini443 – 46624CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. integral component of plasma membrane Source: UniProtKB
  3. postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Muscarinic acetylcholine receptor M2PRO_0000069022Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi2 – 21N-linked (GlcNAc...)Sequence Analysis
Glycosylationi3 – 31N-linked (GlcNAc...)Sequence Analysis
Glycosylationi6 – 61N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi96 ↔ 176PROSITE-ProRule annotation
Disulfide bondi413 ↔ 416PROSITE-ProRule annotation
Modified residuei446 – 4461PhosphothreonineSequence Analysis
Modified residuei450 – 4501PhosphothreonineSequence Analysis
Modified residuei465 – 4651PhosphothreonineSequence Analysis

Post-translational modificationi

Phosphorylated in response to agonist treatment.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9ERZ4.
PRIDEiQ9ERZ4.

PTM databases

PhosphoSiteiQ9ERZ4.

Expressioni

Gene expression databases

BgeeiQ9ERZ4.
CleanExiMM_CHRM2.
GenevestigatoriQ9ERZ4.

Interactioni

Subunit structurei

Interacts with ARRB1 and ARRB2. Interacts with GNB2L1/RACK1; the interaction regulates CHRM2 internalization (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9ERZ4.
SMRiQ9ERZ4. Positions 20-217, 377-456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni103 – 1075Agonist bindingBy similarity
Regioni403 – 4042Agonist bindingBy similarity
Regioni426 – 4305Agonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi120 – 1223Important for signaling
Motifi436 – 4405Important for signaling

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00770000120457.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiQ9ERZ4.
KOiK04130.
OMAiDGGLEHN.
OrthoDBiEOG7V49Z7.
PhylomeDBiQ9ERZ4.
TreeFamiTF320495.

Family and domain databases

Gene3Di1.20.1070.10. 2 hits.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_Ach_M2_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF57. PTHR24249:SF57. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ERZ4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNNSTNSSNN GLAITSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV
60 70 80 90 100
NRHLQTVNNY FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL
110 120 130 140 150
ALDYVVSNAS VMNLLIISFD RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL
160 170 180 190 200
SFILWAPAIL FWQFIVGVRT VEDGECYIQF FSNAAVTFGT AIAAFYLPVI
210 220 230 240 250
IMTVLYWHIS RASKSRIKKE KKEPVANQDP VSPSLVQGRI VKPNNNNMPG
260 270 280 290 300
GDGGLEHNKI QNGKAPRDGG TENCVQGEEK ESSNDSTSVS AVASNMRDDE
310 320 330 340 350
ITQDENTVST SLGHSKDDNS RQTCIKIVTK TQKGDACTPT STTVELVGSS
360 370 380 390 400
GQNGDEKQNI VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW
410 420 430 440 450
APYNVMVLIN TFCAPCIPNT VWTIGYWLCY INSTINPACY ALCNATFKKT
460
FKHLLMCHYK NIGATR
Length:466
Mass (Da):51,499
Last modified:July 27, 2011 - v2
Checksum:i93F90B639CB45AE3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti266 – 2661P → L in AAG14343. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264049 Genomic DNA. Translation: AAG14343.1.
AC113246 Genomic DNA. No translation available.
AC159505 Genomic DNA. No translation available.
CCDSiCCDS20004.1.
RefSeqiNP_987076.2. NM_203491.3.
XP_006506199.1. XM_006506136.1.
XP_006506200.1. XM_006506137.1.
UniGeneiMm.448632.

Genome annotation databases

EnsembliENSMUST00000172278; ENSMUSP00000130874; ENSMUSG00000045613.
GeneIDi243764.
KEGGimmu:243764.
UCSCiuc009biy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264049 Genomic DNA. Translation: AAG14343.1 .
AC113246 Genomic DNA. No translation available.
AC159505 Genomic DNA. No translation available.
CCDSi CCDS20004.1.
RefSeqi NP_987076.2. NM_203491.3.
XP_006506199.1. XM_006506136.1.
XP_006506200.1. XM_006506137.1.
UniGenei Mm.448632.

3D structure databases

ProteinModelPortali Q9ERZ4.
SMRi Q9ERZ4. Positions 20-217, 377-456.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q9ERZ4.
ChEMBLi CHEMBL2097162.
GuidetoPHARMACOLOGYi 14.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei Q9ERZ4.

Proteomic databases

PaxDbi Q9ERZ4.
PRIDEi Q9ERZ4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000172278 ; ENSMUSP00000130874 ; ENSMUSG00000045613 .
GeneIDi 243764.
KEGGi mmu:243764.
UCSCi uc009biy.2. mouse.

Organism-specific databases

CTDi 1129.
MGIi MGI:88397. Chrm2.

Phylogenomic databases

eggNOGi NOG250863.
GeneTreei ENSGT00770000120457.
HOGENOMi HOG000231484.
HOVERGENi HBG105720.
InParanoidi Q9ERZ4.
KOi K04130.
OMAi DGGLEHN.
OrthoDBi EOG7V49Z7.
PhylomeDBi Q9ERZ4.
TreeFami TF320495.

Enzyme and pathway databases

Reactomei REACT_240390. Muscarinic acetylcholine receptors.
REACT_250376. G alpha (i) signalling events.

Miscellaneous databases

NextBioi 385914.
PROi Q9ERZ4.
SOURCEi Search...

Gene expression databases

Bgeei Q9ERZ4.
CleanExi MM_CHRM2.
Genevestigatori Q9ERZ4.

Family and domain databases

Gene3Di 1.20.1070.10. 2 hits.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_Ach_M2_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view ]
PANTHERi PTHR24249:SF57. PTHR24249:SF57. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, sequence and functional expression of mouse muscarinic acetylcholine receptor genes."
    Gomeza J., Wess J.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiACM2_MOUSE
AccessioniPrimary (citable) accession number: Q9ERZ4
Secondary accession number(s): E9Q9P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3