Muscarinic acetylcholine receptor M2

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Q9ERZ4 (ACM2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 90. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Muscarinic acetylcholine receptor M2

Gene names

Name:	Chrm2
Synonyms:	Chrm-2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Subunit structure	Interacts with ARRB1 and ARRB2. Interacts with GNB2L1/RACK1; the interaction regulates CHRM2 internalization By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to virus Inferred from electronic annotation. Source: Compara
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled acetylcholine receptor activity Inferred from mutant phenotype PubMed 9990086. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 466

466

Muscarinic acetylcholine receptor M2

PRO_0000069022

Regions

Topological domain

1 – 22

Extracellular By similarity

Transmembrane

23 – 45

Helical; Name=1; By similarity

Topological domain

46 – 59

Cytoplasmic By similarity

Transmembrane

60 – 80

Helical; Name=2; By similarity

Topological domain

81 – 97

Extracellular By similarity

Transmembrane

98 – 119

Helical; Name=3; By similarity

Topological domain

120 – 139

Cytoplasmic By similarity

Transmembrane

140 – 162

Helical; Name=4; By similarity

Topological domain

163 – 184

Extracellular By similarity

Transmembrane

185 – 207

Helical; Name=5; By similarity

Topological domain

208 – 388

181

Cytoplasmic By similarity

Transmembrane

389 – 409

Helical; Name=6; By similarity

Topological domain

410 – 423

Extracellular By similarity

Transmembrane

424 – 443

Helical; Name=7; By similarity

Topological domain

444 – 466

Cytoplasmic By similarity

Amino acid modifications

Modified residue

232

Phosphoserine Ref.3

Modified residue

234

Phosphoserine Ref.3

Modified residue

446

Phosphothreonine Potential

Modified residue

450

Phosphothreonine Potential

Modified residue

465

Phosphothreonine Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Disulfide bond

96 ↔ 176

By similarity

Experimental info

Sequence conflict

266

P → L in AAG14343. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q9ERZ4 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 93F90B639CB45AE3
Show »

FASTA

466

51,499

        10         20         30         40         50         60 
MNNSTNSSNN GLAITSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV NRHLQTVNNY 

        70         80         90        100        110        120 
FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL ALDYVVSNAS VMNLLIISFD 

       130        140        150        160        170        180 
RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL SFILWAPAIL FWQFIVGVRT VEDGECYIQF 

       190        200        210        220        230        240 
FSNAAVTFGT AIAAFYLPVI IMTVLYWHIS RASKSRIKKE KKEPVANQDP VSPSLVQGRI 

       250        260        270        280        290        300 
VKPNNNNMPG GDGGLEHNKI QNGKAPRDGG TENCVQGEEK ESSNDSTSVS AVASNMRDDE 

       310        320        330        340        350        360 
ITQDENTVST SLGHSKDDNS RQTCIKIVTK TQKGDACTPT STTVELVGSS GQNGDEKQNI 

       370        380        390        400        410        420 
VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW APYNVMVLIN TFCAPCIPNT 

       430        440        450        460 
VWTIGYWLCY INSTINPACY ALCNATFKKT FKHLLMCHYK NIGATR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, sequence and functional expression of mouse muscarinic acetylcholine receptor genes." Gomeza J., Wess J. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232 AND SER-234, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF264049 Genomic DNA. Translation: AAG14343.1. AC113246 Genomic DNA. No translation available. AC159505 Genomic DNA. No translation available.
IPI	IPI00112942.
RefSeq	NP_987076.2. NM_203491.3.
UniGene	Mm.448632.
3D structure databases
ProteinModelPortal	Q9ERZ4.
SMR	Q9ERZ4. Positions 20-456.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9ERZ4.
Proteomic databases
PaxDb	Q9ERZ4.
PRIDE	Q9ERZ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000172278; ENSMUSP00000130874; ENSMUSG00000045613.
GeneID	243764.
KEGG	mmu:243764.
UCSC	uc009biy.2. mouse.
Organism-specific databases
CTD	1129.
MGI	MGI:88397. Chrm2.
Phylogenomic databases
eggNOG	NOG250863.
GeneTree	ENSGT00690000101881.
HOGENOM	HOG000231484.
HOVERGEN	HBG105720.
InParanoid	Q9ERZ4.
KO	K04130.
OMA	DGGLEHN.
OrthoDB	EOG41JZCC.
Gene expression databases
Bgee	Q9ERZ4.
CleanEx	MM_CHRM2.
Genevestigator	Q9ERZ4.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001065. Musac_M2_rcpt. IPR000995. Musac_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00243. MUSCARINICR. PR00539. MUSCRINICM2R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9ERZ4.
ChEMBL	CHEMBL3197.
NextBio	385914.
SOURCE	Search...

Entry information

Entry name

ACM2_MOUSE

Accession

Primary (citable) accession number: Q9ERZ4
Secondary accession number(s): E9Q9P0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents