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Protein

Muscarinic acetylcholine receptor M3

Gene

Chrm3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

GO - Molecular functioni

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway Source: GO_Central
  • digestion Source: MGI
  • G-protein coupled acetylcholine receptor signaling pathway Source: UniProtKB
  • phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: MGI
  • positive regulation of smooth muscle contraction Source: MGI
  • regulation of insulin secretion Source: Reactome
  • regulation of vascular smooth muscle contraction Source: GO_Central
  • saliva secretion Source: InterPro
  • smooth muscle contraction Source: MGI
  • synaptic transmission, cholinergic Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-390648. Muscarinic acetylcholine receptors.
R-MMU-399997. Acetylcholine regulates insulin secretion.
R-MMU-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M3
Alternative name(s):
Mm3 mAChR
Gene namesi
Name:Chrm3
Synonyms:Chrm-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:88398. Chrm3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 66ExtracellularBy similarityAdd BLAST66
Transmembranei67 – 90Helical; Name=1By similarityAdd BLAST24
Topological domaini91 – 103CytoplasmicBy similarityAdd BLAST13
Transmembranei104 – 129Helical; Name=2By similarityAdd BLAST26
Topological domaini130 – 141ExtracellularBy similarityAdd BLAST12
Transmembranei142 – 163Helical; Name=3By similarityAdd BLAST22
Topological domaini164 – 183CytoplasmicBy similarityAdd BLAST20
Transmembranei184 – 205Helical; Name=4By similarityAdd BLAST22
Topological domaini206 – 228ExtracellularBy similarityAdd BLAST23
Transmembranei229 – 251Helical; Name=5By similarityAdd BLAST23
Topological domaini252 – 490CytoplasmicBy similarityAdd BLAST239
Transmembranei491 – 513Helical; Name=6By similarityAdd BLAST23
Topological domaini514 – 525ExtracellularBy similarityAdd BLAST12
Transmembranei526 – 545Helical; Name=7By similarityAdd BLAST20
Topological domaini546 – 589CytoplasmicBy similarityAdd BLAST44

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5105.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690301 – 589Muscarinic acetylcholine receptor M3Add BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi6N-linked (GlcNAc...)Sequence analysis1
Glycosylationi15N-linked (GlcNAc...)Sequence analysis1
Glycosylationi41N-linked (GlcNAc...)Sequence analysis1
Glycosylationi48N-linked (GlcNAc...)Sequence analysis1
Glycosylationi52N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi140 ↔ 220PROSITE-ProRule annotation
Modified residuei384PhosphoserineCombined sources1
Disulfide bondi516 ↔ 519PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9ERZ3.
PaxDbiQ9ERZ3.
PRIDEiQ9ERZ3.

PTM databases

iPTMnetiQ9ERZ3.
PhosphoSitePlusiQ9ERZ3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000046159.
CleanExiMM_CHRM3.
ExpressionAtlasiQ9ERZ3. baseline and differential.
GenevisibleiQ9ERZ3. MM.

Interactioni

Subunit structurei

Homodimer; the dimers can form tetramers (By similarity). Interacts with NALCN (PubMed:19575010).By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055579.

Chemistry databases

BindingDBiQ9ERZ3.

Structurei

3D structure databases

ProteinModelPortaliQ9ERZ3.
SMRiQ9ERZ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 151Agonist bindingBy similarity5
Regioni506 – 529Agonist bindingBy similarityAdd BLAST24

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi274 – 280Basolateral sorting signalBy similarity7

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiQ9ERZ3.
KOiK04131.
OMAiVNTFCNC.
OrthoDBiEOG091G06VI.
PhylomeDBiQ9ERZ3.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF61. PTHR24249:SF61. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ERZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLHSNSTTS PLFPNISSSW VHSPSEAGLP LGTVSQLDSY NISQTSGNFS
60 70 80 90 100
SNDTSSDPLG GHTIWQVVFI AFLTGFLALV TIIGNILVIV AFKVNKQLKT
110 120 130 140 150
VNNYFLLSLA CADLIIGVIS MNLFTTYIIM NRWALGNLAC DLWLSIDYVA
160 170 180 190 200
SNASVMNLLV ISFDRYFSIT RPLTYRAKRT TKRAGVMIGL AWVISFVLWA
210 220 230 240 250
PAILFWQYFV GKRTVPPGEC FIQFLSEPTI TFGTAIAAFY MPVTIMTILY
260 270 280 290 300
WRIYKETEKR TKELAGLQAS GTEAEAENFV HPTGSSRSCS SYELQQQGTK
310 320 330 340 350
RSSRRKYGGC HFWFTTKSWK PSAEQMDQDH SSSDSWNNND AAASLENSAS
360 370 380 390 400
SDEEDIGSET RAIYSIVLKL PGHSTILNST KLPSSDNLQV PDKDLGTMDV
410 420 430 440 450
ERNAHKLQAQ KSMDDRDNCQ KDFSKLPIQL ESAVDTAKTS DTNSSVDKTT
460 470 480 490 500
AALPLSFKEA TLAKRFALKT RSQITKRKRM SLIKEKKAAQ TLSAILLAFI
510 520 530 540 550
ITWTPYNIMV LVNTFCDSCI PKTYWNLGYW LCYINSTVNP VCYALCNKTF
560 570 580
RTTFKMLLLC QCDKRKRRKQ QYQQRQSVIF HKRVPEQAL
Length:589
Mass (Da):66,212
Last modified:March 1, 2001 - v1
Checksum:iAB782149EBEE7804
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264050 Genomic DNA. Translation: AAG14344.1.
S74908 mRNA. Translation: AAB33576.2.
CCDSiCCDS26238.1.
RefSeqiNP_150372.1. NM_033269.4.
XP_006516531.1. XM_006516468.3.
UniGeneiMm.342315.

Genome annotation databases

EnsembliENSMUST00000063093; ENSMUSP00000055579; ENSMUSG00000046159.
ENSMUST00000187510; ENSMUSP00000140131; ENSMUSG00000046159.
GeneIDi12671.
KEGGimmu:12671.
UCSCiuc007plb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264050 Genomic DNA. Translation: AAG14344.1.
S74908 mRNA. Translation: AAB33576.2.
CCDSiCCDS26238.1.
RefSeqiNP_150372.1. NM_033269.4.
XP_006516531.1. XM_006516468.3.
UniGeneiMm.342315.

3D structure databases

ProteinModelPortaliQ9ERZ3.
SMRiQ9ERZ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055579.

Chemistry databases

BindingDBiQ9ERZ3.
ChEMBLiCHEMBL5105.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ9ERZ3.
PhosphoSitePlusiQ9ERZ3.

Proteomic databases

MaxQBiQ9ERZ3.
PaxDbiQ9ERZ3.
PRIDEiQ9ERZ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063093; ENSMUSP00000055579; ENSMUSG00000046159.
ENSMUST00000187510; ENSMUSP00000140131; ENSMUSG00000046159.
GeneIDi12671.
KEGGimmu:12671.
UCSCiuc007plb.1. mouse.

Organism-specific databases

CTDi1131.
MGIiMGI:88398. Chrm3.

Phylogenomic databases

eggNOGiKOG4220. Eukaryota.
ENOG410YCQR. LUCA.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiQ9ERZ3.
KOiK04131.
OMAiVNTFCNC.
OrthoDBiEOG091G06VI.
PhylomeDBiQ9ERZ3.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiR-MMU-390648. Muscarinic acetylcholine receptors.
R-MMU-399997. Acetylcholine regulates insulin secretion.
R-MMU-416476. G alpha (q) signalling events.

Miscellaneous databases

PROiQ9ERZ3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046159.
CleanExiMM_CHRM3.
ExpressionAtlasiQ9ERZ3. baseline and differential.
GenevisibleiQ9ERZ3. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF61. PTHR24249:SF61. 3 hits.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACM3_MOUSE
AccessioniPrimary (citable) accession number: Q9ERZ3
Secondary accession number(s): Q64055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.