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Protein

Muscarinic acetylcholine receptor M3

Gene

Chrm3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

GO - Molecular functioni

  1. acetylcholine binding Source: UniProtKB
  2. acetylcholine receptor activity Source: MGI
  3. drug binding Source: Ensembl
  4. G-protein coupled acetylcholine receptor activity Source: UniProtKB
  5. guanyl-nucleotide exchange factor activity Source: Reactome

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway Source: GO_Central
  2. digestion Source: MGI
  3. G-protein coupled acetylcholine receptor signaling pathway Source: UniProtKB
  4. phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: MGI
  5. positive regulation of GTPase activity Source: GOC
  6. positive regulation of smooth muscle contraction Source: MGI
  7. regulation of vascular smooth muscle contraction Source: GO_Central
  8. saliva secretion Source: InterPro
  9. sensory perception of chemical stimulus Source: GO_Central
  10. smooth muscle contraction Source: MGI
  11. synaptic transmission, cholinergic Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_279397. Muscarinic acetylcholine receptors.
REACT_306601. Acetylcholine regulates insulin secretion.
REACT_347934. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M3
Alternative name(s):
Mm3 mAChR
Gene namesi
Name:Chrm3
Synonyms:Chrm-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:88398. Chrm3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6666ExtracellularBy similarityAdd
BLAST
Transmembranei67 – 9024Helical; Name=1By similarityAdd
BLAST
Topological domaini91 – 10313CytoplasmicBy similarityAdd
BLAST
Transmembranei104 – 12926Helical; Name=2By similarityAdd
BLAST
Topological domaini130 – 14112ExtracellularBy similarityAdd
BLAST
Transmembranei142 – 16322Helical; Name=3By similarityAdd
BLAST
Topological domaini164 – 18320CytoplasmicBy similarityAdd
BLAST
Transmembranei184 – 20522Helical; Name=4By similarityAdd
BLAST
Topological domaini206 – 22823ExtracellularBy similarityAdd
BLAST
Transmembranei229 – 25123Helical; Name=5By similarityAdd
BLAST
Topological domaini252 – 490239CytoplasmicBy similarityAdd
BLAST
Transmembranei491 – 51323Helical; Name=6By similarityAdd
BLAST
Topological domaini514 – 52512ExtracellularBy similarityAdd
BLAST
Transmembranei526 – 54520Helical; Name=7By similarityAdd
BLAST
Topological domaini546 – 58944CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. asymmetric synapse Source: Ensembl
  2. axon terminus Source: Ensembl
  3. basolateral plasma membrane Source: UniProtKB-SubCell
  4. cell junction Source: UniProtKB-KW
  5. dendrite Source: Ensembl
  6. integral component of plasma membrane Source: UniProtKB
  7. plasma membrane Source: Reactome
  8. postsynaptic membrane Source: UniProtKB-SubCell
  9. synapse Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 589589Muscarinic acetylcholine receptor M3PRO_0000069030Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi6 – 61N-linked (GlcNAc...)Sequence Analysis
Glycosylationi15 – 151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi48 – 481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi52 – 521N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi140 ↔ 220PROSITE-ProRule annotation
Disulfide bondi516 ↔ 519PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9ERZ3.
PaxDbiQ9ERZ3.
PRIDEiQ9ERZ3.

PTM databases

PhosphoSiteiQ9ERZ3.

Expressioni

Gene expression databases

BgeeiQ9ERZ3.
CleanExiMM_CHRM3.
ExpressionAtlasiQ9ERZ3. baseline and differential.
GenevestigatoriQ9ERZ3.

Interactioni

Subunit structurei

Homodimer; the dimers can form tetramers.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055579.

Structurei

3D structure databases

ProteinModelPortaliQ9ERZ3.
SMRiQ9ERZ3. Positions 43-259, 399-559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni147 – 1515Agonist bindingBy similarity
Regioni506 – 52924Agonist bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi274 – 2807Basolateral sorting signalBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiQ9ERZ3.
KOiK04131.
OMAiVNTFCNC.
OrthoDBiEOG7V49Z7.
PhylomeDBiQ9ERZ3.
TreeFamiTF320495.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF61. PTHR24249:SF61. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ERZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLHSNSTTS PLFPNISSSW VHSPSEAGLP LGTVSQLDSY NISQTSGNFS
60 70 80 90 100
SNDTSSDPLG GHTIWQVVFI AFLTGFLALV TIIGNILVIV AFKVNKQLKT
110 120 130 140 150
VNNYFLLSLA CADLIIGVIS MNLFTTYIIM NRWALGNLAC DLWLSIDYVA
160 170 180 190 200
SNASVMNLLV ISFDRYFSIT RPLTYRAKRT TKRAGVMIGL AWVISFVLWA
210 220 230 240 250
PAILFWQYFV GKRTVPPGEC FIQFLSEPTI TFGTAIAAFY MPVTIMTILY
260 270 280 290 300
WRIYKETEKR TKELAGLQAS GTEAEAENFV HPTGSSRSCS SYELQQQGTK
310 320 330 340 350
RSSRRKYGGC HFWFTTKSWK PSAEQMDQDH SSSDSWNNND AAASLENSAS
360 370 380 390 400
SDEEDIGSET RAIYSIVLKL PGHSTILNST KLPSSDNLQV PDKDLGTMDV
410 420 430 440 450
ERNAHKLQAQ KSMDDRDNCQ KDFSKLPIQL ESAVDTAKTS DTNSSVDKTT
460 470 480 490 500
AALPLSFKEA TLAKRFALKT RSQITKRKRM SLIKEKKAAQ TLSAILLAFI
510 520 530 540 550
ITWTPYNIMV LVNTFCDSCI PKTYWNLGYW LCYINSTVNP VCYALCNKTF
560 570 580
RTTFKMLLLC QCDKRKRRKQ QYQQRQSVIF HKRVPEQAL
Length:589
Mass (Da):66,212
Last modified:March 1, 2001 - v1
Checksum:iAB782149EBEE7804
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264050 Genomic DNA. Translation: AAG14344.1.
S74908 mRNA. Translation: AAB33576.2.
CCDSiCCDS26238.1.
RefSeqiNP_150372.1. NM_033269.4.
XP_006516531.1. XM_006516468.2.
UniGeneiMm.342315.

Genome annotation databases

EnsembliENSMUST00000063093; ENSMUSP00000055579; ENSMUSG00000046159.
ENSMUST00000187510; ENSMUSP00000140131; ENSMUSG00000046159.
GeneIDi12671.
KEGGimmu:12671.
UCSCiuc007plb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264050 Genomic DNA. Translation: AAG14344.1.
S74908 mRNA. Translation: AAB33576.2.
CCDSiCCDS26238.1.
RefSeqiNP_150372.1. NM_033269.4.
XP_006516531.1. XM_006516468.2.
UniGeneiMm.342315.

3D structure databases

ProteinModelPortaliQ9ERZ3.
SMRiQ9ERZ3. Positions 43-259, 399-559.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055579.

Chemistry

BindingDBiQ9ERZ3.
ChEMBLiCHEMBL2097162.
GuidetoPHARMACOLOGYi15.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9ERZ3.

Proteomic databases

MaxQBiQ9ERZ3.
PaxDbiQ9ERZ3.
PRIDEiQ9ERZ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063093; ENSMUSP00000055579; ENSMUSG00000046159.
ENSMUST00000187510; ENSMUSP00000140131; ENSMUSG00000046159.
GeneIDi12671.
KEGGimmu:12671.
UCSCiuc007plb.1. mouse.

Organism-specific databases

CTDi1131.
MGIiMGI:88398. Chrm3.

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00780000121874.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiQ9ERZ3.
KOiK04131.
OMAiVNTFCNC.
OrthoDBiEOG7V49Z7.
PhylomeDBiQ9ERZ3.
TreeFamiTF320495.

Enzyme and pathway databases

ReactomeiREACT_279397. Muscarinic acetylcholine receptors.
REACT_306601. Acetylcholine regulates insulin secretion.
REACT_347934. G alpha (q) signalling events.

Miscellaneous databases

NextBioi281910.
PROiQ9ERZ3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9ERZ3.
CleanExiMM_CHRM3.
ExpressionAtlasiQ9ERZ3. baseline and differential.
GenevestigatoriQ9ERZ3.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF61. PTHR24249:SF61. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation, sequence and functional expression of mouse muscarinic acetylcholine receptor genes."
    Gomeza J., Wess J.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cultured neurons from mouse brain reproduce the muscarinic receptor profile of their tissue of origin."
    Andre C., Dos Santos G., Koulakoff A.
    Eur. J. Neurosci. 6:1691-1701(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 314-439.
    Tissue: Brain.

Entry informationi

Entry nameiACM3_MOUSE
AccessioniPrimary (citable) accession number: Q9ERZ3
Secondary accession number(s): Q64055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 1, 2001
Last modified: April 29, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.