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Q9ERZ3

- ACM3_MOUSE

UniProt

Q9ERZ3 - ACM3_MOUSE

Protein

Muscarinic acetylcholine receptor M3

Gene

Chrm3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

    GO - Molecular functioni

    1. acetylcholine binding Source: UniProtKB
    2. acetylcholine receptor activity Source: MGI
    3. drug binding Source: Ensembl
    4. G-protein coupled acetylcholine receptor activity Source: UniProtKB
    5. guanyl-nucleotide exchange factor activity Source: Reactome

    GO - Biological processi

    1. digestion Source: MGI
    2. G-protein coupled acetylcholine receptor signaling pathway Source: UniProtKB
    3. phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: MGI
    4. positive regulation of GTPase activity Source: GOC
    5. positive regulation of smooth muscle contraction Source: MGI
    6. regulation of vascular smooth muscle contraction Source: Ensembl
    7. saliva secretion Source: InterPro
    8. smooth muscle contraction Source: MGI
    9. synaptic transmission, cholinergic Source: MGI

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_207651. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Muscarinic acetylcholine receptor M3
    Alternative name(s):
    Mm3 mAChR
    Gene namesi
    Name:Chrm3
    Synonyms:Chrm-3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:88398. Chrm3.

    Subcellular locationi

    GO - Cellular componenti

    1. asymmetric synapse Source: Ensembl
    2. axon terminus Source: Ensembl
    3. basolateral plasma membrane Source: UniProtKB-SubCell
    4. cell junction Source: UniProtKB-KW
    5. dendrite Source: Ensembl
    6. integral component of plasma membrane Source: UniProtKB
    7. plasma membrane Source: Reactome
    8. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 589589Muscarinic acetylcholine receptor M3PRO_0000069030Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi6 – 61N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi15 – 151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi48 – 481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi52 – 521N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi140 ↔ 220PROSITE-ProRule annotation
    Disulfide bondi516 ↔ 519PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ9ERZ3.
    PRIDEiQ9ERZ3.

    PTM databases

    PhosphoSiteiQ9ERZ3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9ERZ3.
    BgeeiQ9ERZ3.
    CleanExiMM_CHRM3.
    GenevestigatoriQ9ERZ3.

    Interactioni

    Subunit structurei

    Homodimer; the dimers can form tetramers.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000055579.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ERZ3.
    SMRiQ9ERZ3. Positions 63-259, 399-559.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6666ExtracellularBy similarityAdd
    BLAST
    Topological domaini91 – 10313CytoplasmicBy similarityAdd
    BLAST
    Topological domaini130 – 14112ExtracellularBy similarityAdd
    BLAST
    Topological domaini164 – 18320CytoplasmicBy similarityAdd
    BLAST
    Topological domaini206 – 22823ExtracellularBy similarityAdd
    BLAST
    Topological domaini252 – 490239CytoplasmicBy similarityAdd
    BLAST
    Topological domaini514 – 52512ExtracellularBy similarityAdd
    BLAST
    Topological domaini546 – 58944CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei67 – 9024Helical; Name=1By similarityAdd
    BLAST
    Transmembranei104 – 12926Helical; Name=2By similarityAdd
    BLAST
    Transmembranei142 – 16322Helical; Name=3By similarityAdd
    BLAST
    Transmembranei184 – 20522Helical; Name=4By similarityAdd
    BLAST
    Transmembranei229 – 25123Helical; Name=5By similarityAdd
    BLAST
    Transmembranei491 – 51323Helical; Name=6By similarityAdd
    BLAST
    Transmembranei526 – 54520Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni147 – 1515Agonist bindingBy similarity
    Regioni506 – 52924Agonist bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi274 – 2807Basolateral sorting signalBy similarity

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG250863.
    GeneTreeiENSGT00690000101881.
    HOGENOMiHOG000231484.
    HOVERGENiHBG105720.
    InParanoidiQ9ERZ3.
    KOiK04131.
    OMAiIWQVVFI.
    OrthoDBiEOG7V49Z7.
    PhylomeDBiQ9ERZ3.
    TreeFamiTF320495.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001183. Musac_Ach_M3_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view]
    PANTHERiPTHR24249:SF61. PTHR24249:SF61. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00540. MUSCRINICM3R.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ERZ3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLHSNSTTS PLFPNISSSW VHSPSEAGLP LGTVSQLDSY NISQTSGNFS    50
    SNDTSSDPLG GHTIWQVVFI AFLTGFLALV TIIGNILVIV AFKVNKQLKT 100
    VNNYFLLSLA CADLIIGVIS MNLFTTYIIM NRWALGNLAC DLWLSIDYVA 150
    SNASVMNLLV ISFDRYFSIT RPLTYRAKRT TKRAGVMIGL AWVISFVLWA 200
    PAILFWQYFV GKRTVPPGEC FIQFLSEPTI TFGTAIAAFY MPVTIMTILY 250
    WRIYKETEKR TKELAGLQAS GTEAEAENFV HPTGSSRSCS SYELQQQGTK 300
    RSSRRKYGGC HFWFTTKSWK PSAEQMDQDH SSSDSWNNND AAASLENSAS 350
    SDEEDIGSET RAIYSIVLKL PGHSTILNST KLPSSDNLQV PDKDLGTMDV 400
    ERNAHKLQAQ KSMDDRDNCQ KDFSKLPIQL ESAVDTAKTS DTNSSVDKTT 450
    AALPLSFKEA TLAKRFALKT RSQITKRKRM SLIKEKKAAQ TLSAILLAFI 500
    ITWTPYNIMV LVNTFCDSCI PKTYWNLGYW LCYINSTVNP VCYALCNKTF 550
    RTTFKMLLLC QCDKRKRRKQ QYQQRQSVIF HKRVPEQAL 589
    Length:589
    Mass (Da):66,212
    Last modified:March 1, 2001 - v1
    Checksum:iAB782149EBEE7804
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF264050 Genomic DNA. Translation: AAG14344.1.
    S74908 mRNA. Translation: AAB33576.2.
    CCDSiCCDS26238.1.
    RefSeqiNP_150372.1. NM_033269.4.
    XP_006516531.1. XM_006516468.1.
    UniGeneiMm.342315.

    Genome annotation databases

    EnsembliENSMUST00000063093; ENSMUSP00000055579; ENSMUSG00000046159.
    GeneIDi12671.
    KEGGimmu:12671.
    UCSCiuc007plb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF264050 Genomic DNA. Translation: AAG14344.1 .
    S74908 mRNA. Translation: AAB33576.2 .
    CCDSi CCDS26238.1.
    RefSeqi NP_150372.1. NM_033269.4.
    XP_006516531.1. XM_006516468.1.
    UniGenei Mm.342315.

    3D structure databases

    ProteinModelPortali Q9ERZ3.
    SMRi Q9ERZ3. Positions 63-259, 399-559.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000055579.

    Chemistry

    BindingDBi Q9ERZ3.
    ChEMBLi CHEMBL5105.
    GuidetoPHARMACOLOGYi 15.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei Q9ERZ3.

    Proteomic databases

    PaxDbi Q9ERZ3.
    PRIDEi Q9ERZ3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000063093 ; ENSMUSP00000055579 ; ENSMUSG00000046159 .
    GeneIDi 12671.
    KEGGi mmu:12671.
    UCSCi uc007plb.1. mouse.

    Organism-specific databases

    CTDi 1131.
    MGIi MGI:88398. Chrm3.

    Phylogenomic databases

    eggNOGi NOG250863.
    GeneTreei ENSGT00690000101881.
    HOGENOMi HOG000231484.
    HOVERGENi HBG105720.
    InParanoidi Q9ERZ3.
    KOi K04131.
    OMAi IWQVVFI.
    OrthoDBi EOG7V49Z7.
    PhylomeDBi Q9ERZ3.
    TreeFami TF320495.

    Enzyme and pathway databases

    Reactomei REACT_207651. G alpha (q) signalling events.

    Miscellaneous databases

    NextBioi 281910.
    PROi Q9ERZ3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9ERZ3.
    Bgeei Q9ERZ3.
    CleanExi MM_CHRM3.
    Genevestigatori Q9ERZ3.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001183. Musac_Ach_M3_rcpt.
    IPR000995. Musac_Ach_rcpt.
    [Graphical view ]
    PANTHERi PTHR24249:SF61. PTHR24249:SF61. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00243. MUSCARINICR.
    PR00540. MUSCRINICM3R.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, sequence and functional expression of mouse muscarinic acetylcholine receptor genes."
      Gomeza J., Wess J.
      Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Cultured neurons from mouse brain reproduce the muscarinic receptor profile of their tissue of origin."
      Andre C., Dos Santos G., Koulakoff A.
      Eur. J. Neurosci. 6:1691-1701(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 314-439.
      Tissue: Brain.

    Entry informationi

    Entry nameiACM3_MOUSE
    AccessioniPrimary (citable) accession number: Q9ERZ3
    Secondary accession number(s): Q64055
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3