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Q9ERZ3 (ACM3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Muscarinic acetylcholine receptor M3
Alternative name(s):
Mm3 mAChR
Gene names
Name:Chrm3
Synonyms:Chrm-3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length589 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein By similarity. Basolateral cell membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.

Ontologies

Keywords
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled acetylcholine receptor signaling pathway

Traceable author statement PubMed 10944224. Source: MGI

digestion

Inferred from mutant phenotype PubMed 10944224. Source: MGI

phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway

Traceable author statement PubMed 10944224. Source: MGI

positive regulation of smooth muscle contraction

Inferred from direct assay PubMed 12925702. Source: MGI

regulation of GTPase activity

Traceable author statement. Source: GOC

regulation of vascular smooth muscle contraction

Inferred from electronic annotation. Source: Ensembl

saliva secretion

Inferred from electronic annotation. Source: InterPro

smooth muscle contraction

Inferred from mutant phenotype PubMed 10944224. Source: MGI

synaptic transmission, cholinergic

Traceable author statement PubMed 10944224. Source: MGI

   Cellular_componentasymmetric synapse

Inferred from electronic annotation. Source: Ensembl

axon terminus

Inferred from electronic annotation. Source: Ensembl

basolateral plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

dendrite

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Traceable author statement PubMed 10944224. Source: MGI

plasma membrane

Traceable author statement. Source: Reactome

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionG-protein coupled acetylcholine receptor activity

Inferred from electronic annotation. Source: Ensembl

acetylcholine binding

Inferred from electronic annotation. Source: Ensembl

acetylcholine receptor activity

Traceable author statement PubMed 10944224. Source: MGI

drug binding

Inferred from electronic annotation. Source: Ensembl

guanyl-nucleotide exchange factor activity

Traceable author statement. Source: Reactome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 589589Muscarinic acetylcholine receptor M3
PRO_0000069030

Regions

Topological domain1 – 6666Extracellular By similarity
Transmembrane67 – 9024Helical; Name=1; By similarity
Topological domain91 – 10313Cytoplasmic By similarity
Transmembrane104 – 12421Helical; Name=2; By similarity
Topological domain125 – 14117Extracellular By similarity
Transmembrane142 – 16322Helical; Name=3; By similarity
Topological domain164 – 18320Cytoplasmic By similarity
Transmembrane184 – 20623Helical; Name=4; By similarity
Topological domain207 – 22822Extracellular By similarity
Transmembrane229 – 25123Helical; Name=5; By similarity
Topological domain252 – 491240Cytoplasmic By similarity
Transmembrane492 – 51221Helical; Name=6; By similarity
Topological domain513 – 52614Extracellular By similarity
Transmembrane527 – 54620Helical; Name=7; By similarity
Topological domain547 – 58943Cytoplasmic By similarity
Motif274 – 2807Basolateral sorting signal By similarity

Amino acid modifications

Glycosylation61N-linked (GlcNAc...) Potential
Glycosylation151N-linked (GlcNAc...) Potential
Glycosylation411N-linked (GlcNAc...) Potential
Glycosylation481N-linked (GlcNAc...) Potential
Glycosylation521N-linked (GlcNAc...) Potential
Disulfide bond140 ↔ 220 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9ERZ3 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: AB782149EBEE7804

FASTA58966,212
        10         20         30         40         50         60 
MTLHSNSTTS PLFPNISSSW VHSPSEAGLP LGTVSQLDSY NISQTSGNFS SNDTSSDPLG 

        70         80         90        100        110        120 
GHTIWQVVFI AFLTGFLALV TIIGNILVIV AFKVNKQLKT VNNYFLLSLA CADLIIGVIS 

       130        140        150        160        170        180 
MNLFTTYIIM NRWALGNLAC DLWLSIDYVA SNASVMNLLV ISFDRYFSIT RPLTYRAKRT 

       190        200        210        220        230        240 
TKRAGVMIGL AWVISFVLWA PAILFWQYFV GKRTVPPGEC FIQFLSEPTI TFGTAIAAFY 

       250        260        270        280        290        300 
MPVTIMTILY WRIYKETEKR TKELAGLQAS GTEAEAENFV HPTGSSRSCS SYELQQQGTK 

       310        320        330        340        350        360 
RSSRRKYGGC HFWFTTKSWK PSAEQMDQDH SSSDSWNNND AAASLENSAS SDEEDIGSET 

       370        380        390        400        410        420 
RAIYSIVLKL PGHSTILNST KLPSSDNLQV PDKDLGTMDV ERNAHKLQAQ KSMDDRDNCQ 

       430        440        450        460        470        480 
KDFSKLPIQL ESAVDTAKTS DTNSSVDKTT AALPLSFKEA TLAKRFALKT RSQITKRKRM 

       490        500        510        520        530        540 
SLIKEKKAAQ TLSAILLAFI ITWTPYNIMV LVNTFCDSCI PKTYWNLGYW LCYINSTVNP 

       550        560        570        580 
VCYALCNKTF RTTFKMLLLC QCDKRKRRKQ QYQQRQSVIF HKRVPEQAL 

« Hide

References

[1]"Isolation, sequence and functional expression of mouse muscarinic acetylcholine receptor genes."
Gomeza J., Wess J.
Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cultured neurons from mouse brain reproduce the muscarinic receptor profile of their tissue of origin."
Andre C., Dos Santos G., Koulakoff A.
Eur. J. Neurosci. 6:1691-1701(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 314-439.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF264050 Genomic DNA. Translation: AAG14344.1.
S74908 mRNA. Translation: AAB33576.2.
CCDSCCDS26238.1.
RefSeqNP_150372.1. NM_033269.4.
XP_006516531.1. XM_006516468.1.
UniGeneMm.342315.

3D structure databases

ProteinModelPortalQ9ERZ3.
SMRQ9ERZ3. Positions 63-259, 399-559.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000055579.

Chemistry

BindingDBQ9ERZ3.
ChEMBLCHEMBL5105.
GuidetoPHARMACOLOGY15.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9ERZ3.

Proteomic databases

PaxDbQ9ERZ3.
PRIDEQ9ERZ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000063093; ENSMUSP00000055579; ENSMUSG00000046159.
GeneID12671.
KEGGmmu:12671.
UCSCuc007plb.1. mouse.

Organism-specific databases

CTD1131.
MGIMGI:88398. Chrm3.

Phylogenomic databases

eggNOGNOG250863.
GeneTreeENSGT00690000101881.
HOGENOMHOG000231484.
HOVERGENHBG105720.
InParanoidQ9ERZ3.
KOK04131.
OMAIWQVVFI.
OrthoDBEOG7V49Z7.
PhylomeDBQ9ERZ3.
TreeFamTF320495.

Enzyme and pathway databases

ReactomeREACT_188937. Metabolism.

Gene expression databases

ArrayExpressQ9ERZ3.
BgeeQ9ERZ3.
CleanExMM_CHRM3.
GenevestigatorQ9ERZ3.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERPTHR24249:SF61. PTHR24249:SF61. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281910.
PROQ9ERZ3.
SOURCESearch...

Entry information

Entry nameACM3_MOUSE
AccessionPrimary (citable) accession number: Q9ERZ3
Secondary accession number(s): Q64055
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries