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Q9ERZ3

- ACM3_MOUSE

UniProt

Q9ERZ3 - ACM3_MOUSE

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Protein

Muscarinic acetylcholine receptor M3

Gene

Chrm3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

GO - Molecular functioni

  1. acetylcholine binding Source: UniProtKB
  2. acetylcholine receptor activity Source: MGI
  3. G-protein coupled acetylcholine receptor activity Source: UniProtKB
  4. guanyl-nucleotide exchange factor activity Source: Reactome

GO - Biological processi

  1. digestion Source: MGI
  2. G-protein coupled acetylcholine receptor signaling pathway Source: UniProtKB
  3. phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: MGI
  4. positive regulation of GTPase activity Source: GOC
  5. positive regulation of smooth muscle contraction Source: MGI
  6. saliva secretion Source: InterPro
  7. smooth muscle contraction Source: MGI
  8. synaptic transmission, cholinergic Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_240390. Muscarinic acetylcholine receptors.
REACT_247495. Acetylcholine regulates insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M3
Alternative name(s):
Mm3 mAChR
Gene namesi
Name:Chrm3
Synonyms:Chrm-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:88398. Chrm3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6666ExtracellularBy similarityAdd
BLAST
Transmembranei67 – 9024Helical; Name=1By similarityAdd
BLAST
Topological domaini91 – 10313CytoplasmicBy similarityAdd
BLAST
Transmembranei104 – 12926Helical; Name=2By similarityAdd
BLAST
Topological domaini130 – 14112ExtracellularBy similarityAdd
BLAST
Transmembranei142 – 16322Helical; Name=3By similarityAdd
BLAST
Topological domaini164 – 18320CytoplasmicBy similarityAdd
BLAST
Transmembranei184 – 20522Helical; Name=4By similarityAdd
BLAST
Topological domaini206 – 22823ExtracellularBy similarityAdd
BLAST
Transmembranei229 – 25123Helical; Name=5By similarityAdd
BLAST
Topological domaini252 – 490239CytoplasmicBy similarityAdd
BLAST
Transmembranei491 – 51323Helical; Name=6By similarityAdd
BLAST
Topological domaini514 – 52512ExtracellularBy similarityAdd
BLAST
Transmembranei526 – 54520Helical; Name=7By similarityAdd
BLAST
Topological domaini546 – 58944CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. integral component of plasma membrane Source: UniProtKB
  3. plasma membrane Source: Reactome
  4. postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 589589Muscarinic acetylcholine receptor M3PRO_0000069030Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi6 – 61N-linked (GlcNAc...)Sequence Analysis
Glycosylationi15 – 151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
Glycosylationi48 – 481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi52 – 521N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi140 ↔ 220PROSITE-ProRule annotation
Disulfide bondi516 ↔ 519PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9ERZ3.
PaxDbiQ9ERZ3.
PRIDEiQ9ERZ3.

PTM databases

PhosphoSiteiQ9ERZ3.

Expressioni

Gene expression databases

BgeeiQ9ERZ3.
CleanExiMM_CHRM3.
ExpressionAtlasiQ9ERZ3. baseline.
GenevestigatoriQ9ERZ3.

Interactioni

Subunit structurei

Homodimer; the dimers can form tetramers.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000055579.

Structurei

3D structure databases

ProteinModelPortaliQ9ERZ3.
SMRiQ9ERZ3. Positions 43-259, 399-559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni147 – 1515Agonist bindingBy similarity
Regioni506 – 52924Agonist bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi274 – 2807Basolateral sorting signalBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG250863.
GeneTreeiENSGT00770000120457.
HOGENOMiHOG000231484.
HOVERGENiHBG105720.
InParanoidiQ9ERZ3.
KOiK04131.
OMAiIWQVVFI.
OrthoDBiEOG7V49Z7.
PhylomeDBiQ9ERZ3.
TreeFamiTF320495.

Family and domain databases

Gene3Di1.20.1070.10. 2 hits.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view]
PANTHERiPTHR24249:SF61. PTHR24249:SF61. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ERZ3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLHSNSTTS PLFPNISSSW VHSPSEAGLP LGTVSQLDSY NISQTSGNFS
60 70 80 90 100
SNDTSSDPLG GHTIWQVVFI AFLTGFLALV TIIGNILVIV AFKVNKQLKT
110 120 130 140 150
VNNYFLLSLA CADLIIGVIS MNLFTTYIIM NRWALGNLAC DLWLSIDYVA
160 170 180 190 200
SNASVMNLLV ISFDRYFSIT RPLTYRAKRT TKRAGVMIGL AWVISFVLWA
210 220 230 240 250
PAILFWQYFV GKRTVPPGEC FIQFLSEPTI TFGTAIAAFY MPVTIMTILY
260 270 280 290 300
WRIYKETEKR TKELAGLQAS GTEAEAENFV HPTGSSRSCS SYELQQQGTK
310 320 330 340 350
RSSRRKYGGC HFWFTTKSWK PSAEQMDQDH SSSDSWNNND AAASLENSAS
360 370 380 390 400
SDEEDIGSET RAIYSIVLKL PGHSTILNST KLPSSDNLQV PDKDLGTMDV
410 420 430 440 450
ERNAHKLQAQ KSMDDRDNCQ KDFSKLPIQL ESAVDTAKTS DTNSSVDKTT
460 470 480 490 500
AALPLSFKEA TLAKRFALKT RSQITKRKRM SLIKEKKAAQ TLSAILLAFI
510 520 530 540 550
ITWTPYNIMV LVNTFCDSCI PKTYWNLGYW LCYINSTVNP VCYALCNKTF
560 570 580
RTTFKMLLLC QCDKRKRRKQ QYQQRQSVIF HKRVPEQAL
Length:589
Mass (Da):66,212
Last modified:March 1, 2001 - v1
Checksum:iAB782149EBEE7804
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264050 Genomic DNA. Translation: AAG14344.1.
S74908 mRNA. Translation: AAB33576.2.
CCDSiCCDS26238.1.
RefSeqiNP_150372.1. NM_033269.4.
XP_006516531.1. XM_006516468.1.
UniGeneiMm.342315.

Genome annotation databases

EnsembliENSMUST00000063093; ENSMUSP00000055579; ENSMUSG00000046159.
ENSMUST00000187510; ENSMUSP00000140131; ENSMUSG00000046159.
GeneIDi12671.
KEGGimmu:12671.
UCSCiuc007plb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF264050 Genomic DNA. Translation: AAG14344.1 .
S74908 mRNA. Translation: AAB33576.2 .
CCDSi CCDS26238.1.
RefSeqi NP_150372.1. NM_033269.4.
XP_006516531.1. XM_006516468.1.
UniGenei Mm.342315.

3D structure databases

ProteinModelPortali Q9ERZ3.
SMRi Q9ERZ3. Positions 43-259, 399-559.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000055579.

Chemistry

BindingDBi Q9ERZ3.
ChEMBLi CHEMBL2097162.
GuidetoPHARMACOLOGYi 15.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei Q9ERZ3.

Proteomic databases

MaxQBi Q9ERZ3.
PaxDbi Q9ERZ3.
PRIDEi Q9ERZ3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000063093 ; ENSMUSP00000055579 ; ENSMUSG00000046159 .
ENSMUST00000187510 ; ENSMUSP00000140131 ; ENSMUSG00000046159 .
GeneIDi 12671.
KEGGi mmu:12671.
UCSCi uc007plb.1. mouse.

Organism-specific databases

CTDi 1131.
MGIi MGI:88398. Chrm3.

Phylogenomic databases

eggNOGi NOG250863.
GeneTreei ENSGT00770000120457.
HOGENOMi HOG000231484.
HOVERGENi HBG105720.
InParanoidi Q9ERZ3.
KOi K04131.
OMAi IWQVVFI.
OrthoDBi EOG7V49Z7.
PhylomeDBi Q9ERZ3.
TreeFami TF320495.

Enzyme and pathway databases

Reactomei REACT_207651. G alpha (q) signalling events.
REACT_240390. Muscarinic acetylcholine receptors.
REACT_247495. Acetylcholine regulates insulin secretion.

Miscellaneous databases

NextBioi 281910.
PROi Q9ERZ3.
SOURCEi Search...

Gene expression databases

Bgeei Q9ERZ3.
CleanExi MM_CHRM3.
ExpressionAtlasi Q9ERZ3. baseline.
Genevestigatori Q9ERZ3.

Family and domain databases

Gene3Di 1.20.1070.10. 2 hits.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001183. Musac_Ach_M3_rcpt.
IPR000995. Musac_Ach_rcpt.
[Graphical view ]
PANTHERi PTHR24249:SF61. PTHR24249:SF61. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00540. MUSCRINICM3R.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation, sequence and functional expression of mouse muscarinic acetylcholine receptor genes."
    Gomeza J., Wess J.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cultured neurons from mouse brain reproduce the muscarinic receptor profile of their tissue of origin."
    Andre C., Dos Santos G., Koulakoff A.
    Eur. J. Neurosci. 6:1691-1701(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 314-439.
    Tissue: Brain.

Entry informationi

Entry nameiACM3_MOUSE
AccessioniPrimary (citable) accession number: Q9ERZ3
Secondary accession number(s): Q64055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 1, 2001
Last modified: November 26, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3