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Protein

Pleckstrin homology domain-containing family A member 2

Gene

Plekha2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane.2 Publications

GO - Molecular functioni

  • 1-phosphatidylinositol binding Source: MGI
  • fibronectin binding Source: MGI
  • laminin binding Source: MGI
  • PDZ domain binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family A member 2
Short name:
PH domain-containing family A member 2
Alternative name(s):
PH domain-containing adaptor PHAD47
Tandem PH domain-containing protein 2
Short name:
TAPP-2
Gene namesi
Name:Plekha2
Synonyms:Tapp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1928144. Plekha2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Nucleus 1 Publication

  • Note: Locates to the plasma membrane after treatments that stimulate the production of PtdIns3,4P2.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538771 – 425Pleckstrin homology domain-containing family A member 2Add BLAST425

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei184PhosphoserineBy similarity1
Modified residuei314PhosphoserineBy similarity1
Modified residuei349PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9ERS5.
MaxQBiQ9ERS5.
PaxDbiQ9ERS5.
PRIDEiQ9ERS5.

PTM databases

iPTMnetiQ9ERS5.
PhosphoSitePlusiQ9ERS5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031557.
ExpressionAtlasiQ9ERS5. baseline and differential.
GenevisibleiQ9ERS5. MM.

Interactioni

Subunit structurei

Binds MPDZ and PTPN13.

Binary interactionsi

WithEntry#Exp.IntActNotes
MPDZO759705EBI-8079166,EBI-821405From a different organism.

GO - Molecular functioni

  • fibronectin binding Source: MGI
  • laminin binding Source: MGI
  • PDZ domain binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ9ERS5. 2 interactors.
MINTiMINT-1773601.
STRINGi10090.ENSMUSP00000066546.

Structurei

Secondary structure

1425
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 17Combined sources4
Beta strandi25 – 33Combined sources9
Turni34 – 37Combined sources4
Beta strandi38 – 44Combined sources7
Turni46 – 48Combined sources3
Beta strandi56 – 60Combined sources5
Turni61 – 63Combined sources3
Beta strandi66 – 69Combined sources4
Turni72 – 74Combined sources3
Beta strandi79 – 85Combined sources7
Beta strandi90 – 94Combined sources5
Helixi98 – 110Combined sources13
Turni111 – 113Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V5PNMR-A1-113[»]
ProteinModelPortaliQ9ERS5.
SMRiQ9ERS5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ERS5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 113PH 1PROSITE-ProRule annotationAdd BLAST107
Domaini198 – 298PH 2PROSITE-ProRule annotationAdd BLAST101

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF2M. Eukaryota.
ENOG410ZQEQ. LUCA.
GeneTreeiENSGT00440000037398.
HOGENOMiHOG000116175.
HOVERGENiHBG053612.
InParanoidiQ9ERS5.
OMAiPQHPKEK.
OrthoDBiEOG091G08U3.
PhylomeDBiQ9ERS5.
TreeFamiTF329516.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ERS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYVDRQNRI CGFLDIEDNE NSGKFLRRYF ILDTQANCLL WYMDNPQNLA
60 70 80 90 100
VGAGAVGSLQ LTYISKVSIA TPKQKPKTPF CFVINALSQR YFLQANDQKD
110 120 130 140 150
LKDWVEALNQ ASKITVPKAG TVPLATEVLK NLTAPPTLEK KPQVAYKTEI
160 170 180 190 200
IGGVVVQTPI SQNGGDGQEG CEPGTHAFLR RSQSYIPTSG CRPSTGPPLI
210 220 230 240 250
KSGYCVKQGN VRKSWKRRFF ALDDFTICYF KCEQDREPLR TIPLKDVLKT
260 270 280 290 300
HECLVKSGDL LMRDNLFEII TTSRTFYVQA DSPEDMHSWI EGIGAAVQAL
310 320 330 340 350
KCHPREPSFS RSISLTRPGS STLTSAPNSI LSRRRPPAEE KRGLCKAPSV
360 370 380 390 400
ASSWQPWTPV PQAEEKPLSV EHAPEDSLFM PNPGESTATG VLASSRVRHR
410 420
SEPQHPKEKP FVFNLDDENI RTSDV
Length:425
Mass (Da):47,380
Last modified:March 1, 2001 - v1
Checksum:i6666F5DB303B2CBA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti240R → H in BAC31224 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF286161 mRNA. Translation: AAG15198.1.
AF418551 mRNA. Translation: AAL57436.1.
AK042324 mRNA. Translation: BAC31224.2.
AK078212 mRNA. Translation: BAC37176.1.
AK088395 mRNA. Translation: BAC40325.1.
BC010215 mRNA. Translation: AAH10215.1.
CCDSiCCDS22198.1.
RefSeqiNP_112547.1. NM_031257.3.
XP_006509290.1. XM_006509227.2.
XP_006509291.1. XM_006509228.3.
XP_006509293.1. XM_006509230.2.
UniGeneiMm.261122.

Genome annotation databases

EnsembliENSMUST00000064883; ENSMUSP00000066546; ENSMUSG00000031557.
ENSMUST00000128715; ENSMUSP00000122564; ENSMUSG00000031557.
GeneIDi83436.
KEGGimmu:83436.
UCSCiuc009lfq.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF286161 mRNA. Translation: AAG15198.1.
AF418551 mRNA. Translation: AAL57436.1.
AK042324 mRNA. Translation: BAC31224.2.
AK078212 mRNA. Translation: BAC37176.1.
AK088395 mRNA. Translation: BAC40325.1.
BC010215 mRNA. Translation: AAH10215.1.
CCDSiCCDS22198.1.
RefSeqiNP_112547.1. NM_031257.3.
XP_006509290.1. XM_006509227.2.
XP_006509291.1. XM_006509228.3.
XP_006509293.1. XM_006509230.2.
UniGeneiMm.261122.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V5PNMR-A1-113[»]
ProteinModelPortaliQ9ERS5.
SMRiQ9ERS5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9ERS5. 2 interactors.
MINTiMINT-1773601.
STRINGi10090.ENSMUSP00000066546.

PTM databases

iPTMnetiQ9ERS5.
PhosphoSitePlusiQ9ERS5.

Proteomic databases

EPDiQ9ERS5.
MaxQBiQ9ERS5.
PaxDbiQ9ERS5.
PRIDEiQ9ERS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064883; ENSMUSP00000066546; ENSMUSG00000031557.
ENSMUST00000128715; ENSMUSP00000122564; ENSMUSG00000031557.
GeneIDi83436.
KEGGimmu:83436.
UCSCiuc009lfq.3. mouse.

Organism-specific databases

CTDi59339.
MGIiMGI:1928144. Plekha2.

Phylogenomic databases

eggNOGiENOG410IF2M. Eukaryota.
ENOG410ZQEQ. LUCA.
GeneTreeiENSGT00440000037398.
HOGENOMiHOG000116175.
HOVERGENiHBG053612.
InParanoidiQ9ERS5.
OMAiPQHPKEK.
OrthoDBiEOG091G08U3.
PhylomeDBiQ9ERS5.
TreeFamiTF329516.

Miscellaneous databases

ChiTaRSiPlekha2. mouse.
EvolutionaryTraceiQ9ERS5.
PROiQ9ERS5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031557.
ExpressionAtlasiQ9ERS5. baseline and differential.
GenevisibleiQ9ERS5. MM.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKHA2_MOUSE
AccessioniPrimary (citable) accession number: Q9ERS5
Secondary accession number(s): Q8BY29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.