Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pleckstrin homology domain-containing family A member 2

Gene

Plekha2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane.2 Publications

GO - Molecular functioni

  • 1-phosphatidylinositol binding Source: MGI
  • fibronectin binding Source: MGI
  • laminin binding Source: MGI
  • PDZ domain binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family A member 2
Short name:
PH domain-containing family A member 2
Alternative name(s):
PH domain-containing adaptor PHAD47
Tandem PH domain-containing protein 2
Short name:
TAPP-2
Gene namesi
Name:Plekha2
Synonyms:Tapp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1928144. Plekha2.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Nucleus 1 Publication

  • Note: Locates to the plasma membrane after treatments that stimulate the production of PtdIns3,4P2.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 425425Pleckstrin homology domain-containing family A member 2PRO_0000053877Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki141 – 141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei184 – 1841PhosphoserineBy similarity
Modified residuei349 – 3491PhosphoserineBy similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9ERS5.
MaxQBiQ9ERS5.
PaxDbiQ9ERS5.
PRIDEiQ9ERS5.

PTM databases

iPTMnetiQ9ERS5.
PhosphoSiteiQ9ERS5.

Expressioni

Gene expression databases

BgeeiQ9ERS5.
ExpressionAtlasiQ9ERS5. baseline and differential.
GenevisibleiQ9ERS5. MM.

Interactioni

Subunit structurei

Binds MPDZ and PTPN13.

Binary interactionsi

WithEntry#Exp.IntActNotes
MPDZO759705EBI-8079166,EBI-821405From a different organism.

GO - Molecular functioni

  • fibronectin binding Source: MGI
  • laminin binding Source: MGI
  • PDZ domain binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ9ERS5. 2 interactions.
MINTiMINT-1773601.
STRINGi10090.ENSMUSP00000066546.

Structurei

Secondary structure

1
425
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 174Combined sources
Beta strandi25 – 339Combined sources
Turni34 – 374Combined sources
Beta strandi38 – 447Combined sources
Turni46 – 483Combined sources
Beta strandi56 – 605Combined sources
Turni61 – 633Combined sources
Beta strandi66 – 694Combined sources
Turni72 – 743Combined sources
Beta strandi79 – 857Combined sources
Beta strandi90 – 945Combined sources
Helixi98 – 11013Combined sources
Turni111 – 1133Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V5PNMR-A1-113[»]
ProteinModelPortaliQ9ERS5.
SMRiQ9ERS5. Positions 1-120, 199-299.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ERS5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 113107PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini198 – 298101PH 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IF2M. Eukaryota.
ENOG410ZQEQ. LUCA.
GeneTreeiENSGT00440000037398.
HOGENOMiHOG000116175.
HOVERGENiHBG053612.
InParanoidiQ9ERS5.
OMAiEPQHPKE.
OrthoDBiEOG7VMP5J.
PhylomeDBiQ9ERS5.
TreeFamiTF329516.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ERS5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYVDRQNRI CGFLDIEDNE NSGKFLRRYF ILDTQANCLL WYMDNPQNLA
60 70 80 90 100
VGAGAVGSLQ LTYISKVSIA TPKQKPKTPF CFVINALSQR YFLQANDQKD
110 120 130 140 150
LKDWVEALNQ ASKITVPKAG TVPLATEVLK NLTAPPTLEK KPQVAYKTEI
160 170 180 190 200
IGGVVVQTPI SQNGGDGQEG CEPGTHAFLR RSQSYIPTSG CRPSTGPPLI
210 220 230 240 250
KSGYCVKQGN VRKSWKRRFF ALDDFTICYF KCEQDREPLR TIPLKDVLKT
260 270 280 290 300
HECLVKSGDL LMRDNLFEII TTSRTFYVQA DSPEDMHSWI EGIGAAVQAL
310 320 330 340 350
KCHPREPSFS RSISLTRPGS STLTSAPNSI LSRRRPPAEE KRGLCKAPSV
360 370 380 390 400
ASSWQPWTPV PQAEEKPLSV EHAPEDSLFM PNPGESTATG VLASSRVRHR
410 420
SEPQHPKEKP FVFNLDDENI RTSDV
Length:425
Mass (Da):47,380
Last modified:March 1, 2001 - v1
Checksum:i6666F5DB303B2CBA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti240 – 2401R → H in BAC31224 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF286161 mRNA. Translation: AAG15198.1.
AF418551 mRNA. Translation: AAL57436.1.
AK042324 mRNA. Translation: BAC31224.2.
AK078212 mRNA. Translation: BAC37176.1.
AK088395 mRNA. Translation: BAC40325.1.
BC010215 mRNA. Translation: AAH10215.1.
CCDSiCCDS22198.1.
RefSeqiNP_112547.1. NM_031257.3.
XP_006509290.1. XM_006509227.1.
XP_006509291.1. XM_006509228.2.
XP_006509293.1. XM_006509230.2.
UniGeneiMm.261122.

Genome annotation databases

EnsembliENSMUST00000064883; ENSMUSP00000066546; ENSMUSG00000031557.
ENSMUST00000128715; ENSMUSP00000122564; ENSMUSG00000031557.
GeneIDi83436.
KEGGimmu:83436.
UCSCiuc009lfq.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF286161 mRNA. Translation: AAG15198.1.
AF418551 mRNA. Translation: AAL57436.1.
AK042324 mRNA. Translation: BAC31224.2.
AK078212 mRNA. Translation: BAC37176.1.
AK088395 mRNA. Translation: BAC40325.1.
BC010215 mRNA. Translation: AAH10215.1.
CCDSiCCDS22198.1.
RefSeqiNP_112547.1. NM_031257.3.
XP_006509290.1. XM_006509227.1.
XP_006509291.1. XM_006509228.2.
XP_006509293.1. XM_006509230.2.
UniGeneiMm.261122.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1V5PNMR-A1-113[»]
ProteinModelPortaliQ9ERS5.
SMRiQ9ERS5. Positions 1-120, 199-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9ERS5. 2 interactions.
MINTiMINT-1773601.
STRINGi10090.ENSMUSP00000066546.

PTM databases

iPTMnetiQ9ERS5.
PhosphoSiteiQ9ERS5.

Proteomic databases

EPDiQ9ERS5.
MaxQBiQ9ERS5.
PaxDbiQ9ERS5.
PRIDEiQ9ERS5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064883; ENSMUSP00000066546; ENSMUSG00000031557.
ENSMUST00000128715; ENSMUSP00000122564; ENSMUSG00000031557.
GeneIDi83436.
KEGGimmu:83436.
UCSCiuc009lfq.3. mouse.

Organism-specific databases

CTDi59339.
MGIiMGI:1928144. Plekha2.

Phylogenomic databases

eggNOGiENOG410IF2M. Eukaryota.
ENOG410ZQEQ. LUCA.
GeneTreeiENSGT00440000037398.
HOGENOMiHOG000116175.
HOVERGENiHBG053612.
InParanoidiQ9ERS5.
OMAiEPQHPKE.
OrthoDBiEOG7VMP5J.
PhylomeDBiQ9ERS5.
TreeFamiTF329516.

Miscellaneous databases

ChiTaRSiPlekha2. mouse.
EvolutionaryTraceiQ9ERS5.
PROiQ9ERS5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9ERS5.
ExpressionAtlasiQ9ERS5. baseline and differential.
GenevisibleiQ9ERS5. MM.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities."
    Dowler S.J., Currie R.A., Campbell D.G., Deak M., Kular G., Downes C.P., Alessi D.R.
    Biochem. J. 351:19-31(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "TAPP1 and TAPP2 are targets of phosphatidylinositol 3-kinase signaling in B cells: sustained plasma membrane recruitment triggered by the B-cell antigen receptor."
    Marshall A.J., Krahn A.K., Ma K., Duronio V., Hou S.
    Mol. Cell. Biol. 22:5479-5491(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: BALB/cJ.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Olfactory bulb and Thymus.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo."
    Kimber W.A., Trinkle-Mulcahy L., Cheung P.C.F., Deak M., Marsden L.J., Kieloch A., Watt S., Javier R.T., Gray A., Downes C.P., Lucocq J.M., Alessi D.R.
    Biochem. J. 361:525-536(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MPDZ.
  6. "Interaction of the protein tyrosine phosphatase PTPL1 with the PtdIns(3,4)P2-binding adaptor protein TAPP1."
    Kimber W.A., Deak M., Prescott A.R., Alessi D.R.
    Biochem. J. 376:525-535(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTPN13, FUNCTION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Liver, Lung and Spleen.
  9. "Solution structure of the N-terminal pleckstrin homology domain of TAPP2 from mouse."
    RIKEN structural genomics initiative (RSGI)
    Submitted (MAY-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 1-113.

Entry informationi

Entry nameiPKHA2_MOUSE
AccessioniPrimary (citable) accession number: Q9ERS5
Secondary accession number(s): Q8BY29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.