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Protein

Nuclear distribution protein nudE-like 1

Gene

Ndel1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL.5 Publications

GO - Molecular functioni

  • alpha-tubulin binding Source: MGI
  • beta-tubulin binding Source: MGI
  • microtubule binding Source: MGI
  • oligopeptidase activity Source: Ensembl

GO - Biological processi

  • activation of GTPase activity Source: MGI
  • cell migration Source: MGI
  • central nervous system neuron axonogenesis Source: MGI
  • centrosome localization Source: MGI
  • cerebral cortex radially oriented cell migration Source: DFLAT
  • chromosome segregation Source: MGI
  • establishment of chromosome localization Source: GO_Central
  • establishment of mitotic spindle orientation Source: GO_Central
  • inner cell mass cell proliferation Source: MGI
  • microtubule cytoskeleton organization Source: MGI
  • microtubule nucleation Source: GO_Central
  • mitotic centrosome separation Source: MGI
  • neurofilament cytoskeleton organization Source: MGI
  • neuron migration Source: MGI
  • neuron projection extension Source: MGI
  • nuclear envelope disassembly Source: MGI
  • positive regulation of axon extension Source: Ensembl
  • positive regulation of axon regeneration Source: Ensembl
  • positive regulation of GTPase activity Source: MGI
  • regulation of intracellular protein transport Source: MGI
  • regulation of microtubule motor activity Source: InterPro
  • regulation of neuron projection development Source: UniProtKB
  • retrograde axonal transport Source: MGI
  • vesicle transport along microtubule Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transport

Enzyme and pathway databases

ReactomeiR-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear distribution protein nudE-like 1
Alternative name(s):
Protein mNudE-like
Short name:
Protein Nudel
Short name:
mNudE-L
Gene namesi
Name:Ndel1
Synonyms:Nudel
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1932915. Ndel1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: MGI
  • axon cytoplasm Source: GOC
  • axon hillock Source: MGI
  • cell leading edge Source: MGI
  • centrosome Source: MGI
  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • kinesin complex Source: GO_Central
  • kinetochore Source: MGI
  • microtubule Source: UniProtKB-KW
  • microtubule associated complex Source: MGI
  • microtubule organizing center Source: MGI
  • neurofilament cytoskeleton Source: MGI
  • nuclear envelope Source: MGI
  • spindle Source: UniProtKB-SubCell
  • synaptic vesicle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi198S → A: Reduces phosphorylation by CDK5 and impairs interaction with YWHAE. Impairs interaction with KATNA1; when associated with A-219 and A-231. 2 Publications1
Mutagenesisi219T → A: Reduces phosphorylation by CDK5 and impairs interaction with YWHAE. Impairs interaction with KATNA1; when associated with A-198 and A-231. 2 Publications1
Mutagenesisi231S → A: Reduces phosphorylation by CDK5 and impairs interaction with YWHAE. Impairs interaction with KATNA1; when associated with A-198 and A-219. 2 Publications1
Mutagenesisi266L → A: Impairs interaction with DISC1. 1 Publication1
Mutagenesisi267E → A: Impairs interaction with DISC1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002402121 – 345Nuclear distribution protein nudE-like 1Add BLAST345

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei215PhosphoserineCombined sources1
Modified residuei219PhosphothreonineCombined sources1
Modified residuei231PhosphoserineBy similarity1
Modified residuei242Phosphoserine; by CDK1By similarity1
Modified residuei245Phosphothreonine; by CDK1 and MAPK1By similarity1
Lipidationi273S-palmitoyl cysteine; by ZDHHC2, ZDHHC3 and ZDHHC7By similarity1
Modified residuei344PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by CDK1 and MAPK1 (By similarity). Phosphorylated in mitosis. Phosphorylated by CDK5. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.By similarity3 Publications
Palmitoylation at Cys-273 reduces affinity for dynein.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9ERR1.
PeptideAtlasiQ9ERR1.
PRIDEiQ9ERR1.

PTM databases

iPTMnetiQ9ERR1.
PhosphoSitePlusiQ9ERR1.
SwissPalmiQ9ERR1.

Expressioni

Tissue specificityi

Expressed in brain, liver, lung and testis (at protein level). Expressed in brain, epididymis, eye, heart, kidney, large intestine, liver, ovary, pancreas, prostate, skeletal muscle, smooth muscle, spleen, submaxillary gland, testis, thymus and thyroid. Within the brain expression is pronounced in the cortex, hippocampus, olfactory bulb, striatum, thalamic and hypothalamic structures and in the molecular layer of the cerebellum. Largely excluded from cortical progenitor cells which express NDE1.4 Publications

Developmental stagei

Expression in the brain is detectable from E7, rises at E15 and E17 and peaks at P5. Enriched in the developing cortex, particularly in neuroblasts of the ventricular zone and postmitotic migrating cortical plate neurons. Interaction with DISC1 in the brain is developmentally regulated, peaking at E17 and decreasing at P16 so as to be undetectable in the adult brain. Expressed in the testis from P12, when zygotene spermatocytes first appear, and expression subsequently rises at P27.5 Publications

Gene expression databases

BgeeiENSMUSG00000018736.
GenevisibleiQ9ERR1. MM.

Interactioni

Subunit structurei

Interacts with dynactin, PCM1 and PCNT. Interacts (via C-terminus) with CENPF (By similarity). Self-associates. Interacts with DISC1, dynein, tubulin gamma, KATNA1, KATNB1, microtubules, PAFAHB1 and YWHAE. Interacts directly with NEFL and indirectly with NEFH. Interacts with ZNF365 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Pafah1b1P630057EBI-646668,EBI-917499
YwhaeP622597EBI-646668,EBI-356480

GO - Molecular functioni

  • alpha-tubulin binding Source: MGI
  • beta-tubulin binding Source: MGI
  • microtubule binding Source: MGI

Protein-protein interaction databases

BioGridi219922. 27 interactors.
DIPiDIP-29553N.
IntActiQ9ERR1. 23 interactors.
MINTiMINT-267613.
STRINGi10090.ENSMUSP00000018880.

Structurei

3D structure databases

ProteinModelPortaliQ9ERR1.
SMRiQ9ERR1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni56 – 166Self-associationAdd BLAST111
Regioni64 – 189Interaction with KATNB11 PublicationAdd BLAST126
Regioni114 – 133Required for interaction with PAFAH1B1By similarityAdd BLAST20
Regioni175 – 345Interaction with CENPFBy similarityAdd BLAST171
Regioni189 – 256Interaction with YWHAE1 PublicationAdd BLAST68
Regioni191 – 345Interaction with NEFL1 PublicationAdd BLAST155
Regioni195 – 256Interaction with KATNA11 PublicationAdd BLAST62
Regioni241 – 280Interaction with DISC1By similarityAdd BLAST40
Regioni256 – 291Required for localization to the centrosome and interaction with dynein, dynactin, tubulin gamma, PCM1 and PCNTBy similarityAdd BLAST36

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili28 – 190Sequence analysisAdd BLAST163

Sequence similaritiesi

Belongs to the nudE family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1853. Eukaryota.
ENOG410XPMP. LUCA.
GeneTreeiENSGT00390000000111.
HOGENOMiHOG000280681.
HOVERGENiHBG082010.
InParanoidiQ9ERR1.
KOiK16739.
OMAiQAFLIMD.
OrthoDBiEOG091G0GBV.
PhylomeDBiQ9ERR1.
TreeFamiTF325693.

Family and domain databases

InterProiIPR033493. NDEL1.
IPR033494. NUDE.
IPR006964. NUDE_dom.
[Graphical view]
PANTHERiPTHR10921. PTHR10921. 1 hit.
PTHR10921:SF0. PTHR10921:SF0. 1 hit.
PfamiPF04880. NUDE_C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ERR1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGEDIPDFS SLKEETAYWK ELSLKYKQSF QEARDELVEF QEGSRELEAE
60 70 80 90 100
LEAQLVQAEQ RNRDLQADNQ RLKYEVEALK EKLEHQYAQS YKQVSVLEDD
110 120 130 140 150
LSQTRAIKEQ LHKYVRELEQ ANDDLERAKR ATIVSLEDFE QRLNQAIERN
160 170 180 190 200
AFLESELDEK ESLLVSVQRL KDEARDLRQE LAVRERQQEV TRKSAPSSPT
210 220 230 240 250
LDCEKMDSAV QASLSLPATP VGKGTENSFP SPKAIPNGFG TSPLTPSARI
260 270 280 290 300
SALNIVGDLL RKVGALESKL AACRNFAKDQ ASRKSYVPGS VNCGVMNSNG
310 320 330 340
PECPRSGRAT FFHKGAVNGF DPAPPPPGLG SSRPSSAPGM LPLSV
Length:345
Mass (Da):38,366
Last modified:June 13, 2006 - v2
Checksum:iCB3059DFD998E9EC
GO
Isoform 2 (identifier: Q9ERR1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     316-345: Missing.

Show »
Length:315
Mass (Da):35,511
Checksum:i648A13A80AB95D11
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302E → K in AAG10061 (PubMed:11163259).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019311316 – 345Missing in isoform 2. 2 PublicationsAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290472 mRNA. Translation: AAG10061.1.
AF323918 mRNA. Translation: AAG42496.1.
AK011168 mRNA. Translation: BAB27443.1.
AL603662 Genomic DNA. Translation: CAI25525.1.
AL603662 Genomic DNA. Translation: CAI25526.1.
BC021434 mRNA. Translation: AAH21434.1.
BC046796 mRNA. Translation: AAH46796.1.
CCDSiCCDS24870.1. [Q9ERR1-1]
RefSeqiNP_076157.2. NM_023668.2. [Q9ERR1-1]
UniGeneiMm.31979.

Genome annotation databases

EnsembliENSMUST00000018880; ENSMUSP00000018880; ENSMUSG00000018736. [Q9ERR1-1]
ENSMUST00000101017; ENSMUSP00000098579; ENSMUSG00000018736. [Q9ERR1-2]
ENSMUST00000108672; ENSMUSP00000104312; ENSMUSG00000018736. [Q9ERR1-2]
GeneIDi83431.
KEGGimmu:83431.
UCSCiuc007joc.3. mouse. [Q9ERR1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290472 mRNA. Translation: AAG10061.1.
AF323918 mRNA. Translation: AAG42496.1.
AK011168 mRNA. Translation: BAB27443.1.
AL603662 Genomic DNA. Translation: CAI25525.1.
AL603662 Genomic DNA. Translation: CAI25526.1.
BC021434 mRNA. Translation: AAH21434.1.
BC046796 mRNA. Translation: AAH46796.1.
CCDSiCCDS24870.1. [Q9ERR1-1]
RefSeqiNP_076157.2. NM_023668.2. [Q9ERR1-1]
UniGeneiMm.31979.

3D structure databases

ProteinModelPortaliQ9ERR1.
SMRiQ9ERR1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219922. 27 interactors.
DIPiDIP-29553N.
IntActiQ9ERR1. 23 interactors.
MINTiMINT-267613.
STRINGi10090.ENSMUSP00000018880.

PTM databases

iPTMnetiQ9ERR1.
PhosphoSitePlusiQ9ERR1.
SwissPalmiQ9ERR1.

Proteomic databases

PaxDbiQ9ERR1.
PeptideAtlasiQ9ERR1.
PRIDEiQ9ERR1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018880; ENSMUSP00000018880; ENSMUSG00000018736. [Q9ERR1-1]
ENSMUST00000101017; ENSMUSP00000098579; ENSMUSG00000018736. [Q9ERR1-2]
ENSMUST00000108672; ENSMUSP00000104312; ENSMUSG00000018736. [Q9ERR1-2]
GeneIDi83431.
KEGGimmu:83431.
UCSCiuc007joc.3. mouse. [Q9ERR1-1]

Organism-specific databases

CTDi81565.
MGIiMGI:1932915. Ndel1.

Phylogenomic databases

eggNOGiKOG1853. Eukaryota.
ENOG410XPMP. LUCA.
GeneTreeiENSGT00390000000111.
HOGENOMiHOG000280681.
HOVERGENiHBG082010.
InParanoidiQ9ERR1.
KOiK16739.
OMAiQAFLIMD.
OrthoDBiEOG091G0GBV.
PhylomeDBiQ9ERR1.
TreeFamiTF325693.

Enzyme and pathway databases

ReactomeiR-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.

Miscellaneous databases

ChiTaRSiNdel1. mouse.
PROiQ9ERR1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018736.
GenevisibleiQ9ERR1. MM.

Family and domain databases

InterProiIPR033493. NDEL1.
IPR033494. NUDE.
IPR006964. NUDE_dom.
[Graphical view]
PANTHERiPTHR10921. PTHR10921. 1 hit.
PTHR10921:SF0. PTHR10921:SF0. 1 hit.
PfamiPF04880. NUDE_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDEL1_MOUSE
AccessioniPrimary (citable) accession number: Q9ERR1
Secondary accession number(s): Q9D0Q4, Q9EPT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: November 2, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.