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Protein

Chondroitin sulfate proteoglycan 5

Gene

Cspg5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation.

Miscellaneous

Different forms of various molecular weight have been observed. Such forms are possibly due to different levels of glycosylation, phosphorylation and/or protein cleavage (By similarity).By similarity

GO - Biological processi

  • axon regeneration Source: RGD
  • regulation of growth Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Growth regulation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitin sulfate proteoglycan 5
Alternative name(s):
Acidic leucine-rich EGF-like domain-containing brain protein
Neuroglycan C
Gene namesi
Name:Cspg5
Synonyms:Caleb, Ngc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2431. Cspg5.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini31 – 428ExtracellularSequence analysisAdd BLAST398
Transmembranei429 – 449HelicalSequence analysisAdd BLAST21
Topological domaini450 – 571CytoplasmicSequence analysisAdd BLAST122

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 301 PublicationAdd BLAST30
ChainiPRO_000004215331 – 571Chondroitin sulfate proteoglycan 5Add BLAST541

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi57N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi76O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi123O-linked (Xyl...) (chondroitin sulfate) serineBy similarity1
Glycosylationi132O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi143O-linked (GalNAc...) serineSequence analysis1
Glycosylationi144O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi153O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi156O-linked (GalNAc...) serineSequence analysis1
Glycosylationi160O-linked (GalNAc...) serineSequence analysis1
Glycosylationi162O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi198O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi240O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi318O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi322O-linked (GalNAc...) threonineSequence analysis1
Glycosylationi372N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi379 ↔ 392By similarity
Disulfide bondi386 ↔ 402By similarity
Disulfide bondi404 ↔ 417By similarity
Modified residuei472PhosphoserineBy similarity1
Modified residuei480PhosphoserineBy similarity1
Modified residuei488PhosphoserineBy similarity1
Modified residuei548PhosphoserineBy similarity1

Post-translational modificationi

N-glycosylated.2 Publications
O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation (By similarity).By similarity
Phosphorylated; in intracellular and extracellular parts.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Proteoglycan

Proteomic databases

PaxDbiQ9ERQ6.
PRIDEiQ9ERQ6.

PTM databases

iPTMnetiQ9ERQ6.
PhosphoSitePlusiQ9ERQ6.

Expressioni

Tissue specificityi

Expressed in cerebral cortex and cerebellum. Expressed in retina (at protein level).4 Publications

Developmental stagei

Expression starts at E16 in the cerebral cortex and increases to reach a maximum 20 days after birth. Then it decreases till adulthood to be expressed half of the peak level. In the retina, expression reaches a maximum at postnatal day 14 (P14). It starts weakly at E16 in the retinal pigment epithelium (RPE). At P0 it is detected in nerve fiber layer (NFL), ganglion cell layer (GCL), inner plexiform layer (IPL) and RPE. At P7, it becomes intense in the NFL and IPL. At P14, expression becomes intense in the area of outer segments (OS) of the photoreceptor cells as well as in RPE, whereas in the inner layers it becomes gradually fainter. From P21 to P42, it decreases in inner retinal layers. OS and RPE still express, whereas expression in the NFL and IPL decreases (at protein level).2 Publications

Inductioni

Up-regulated in nucleus accumbens shell by cocaine administration.1 Publication

Interactioni

Subunit structurei

Interacts with ERBB3 and GOPC. Binds TNR and probably TNC (By similarity).By similarity

Protein-protein interaction databases

BioGridi248393. 1 interactor.
STRINGi10116.ENSRNOP00000028278.

Structurei

3D structure databases

SMRiQ9ERQ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini376 – 418EGF-likeAdd BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni270 – 306Interaction with TNC and TNRBy similarityAdd BLAST37
Regioni447 – 465Interaction with GOPCBy similarityAdd BLAST19

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi146 – 200Pro-richAdd BLAST55
Compositional biasi282 – 289Poly-Glu8

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJTR. Eukaryota.
ENOG410YF9F. LUCA.
HOVERGENiHBG081361.
InParanoidiQ9ERQ6.
KOiK08116.
PhylomeDBiQ9ERQ6.

Family and domain databases

InterProiView protein in InterPro
IPR010555. CSPG5_S_attach_dom.
IPR009505. Neural_ProG_Cyt.
PfamiView protein in Pfam
PF06566. Chon_Sulph_att. 1 hit.
PF06567. Neural_ProG_Cyt. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ERQ6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRAGGGGPG WGPPPVLLLL GVTLVLTAGA VPAREAGSAI EAEELVRSGL
60 70 80 90 100
AWESRANDTR EEAGLPAAGE DETSWTERGS ELAAVGPGVG PEETLEASAA
110 120 130 140 150
VTGTAWLEAD GTGLGGVTAE AGSGDAQTLP ATLQAPDEAL GSSTMPPAIP
160 170 180 190 200
EATEASGPPS PTLRDKPSLV PELPKEIPLE VWLNLGGSTP DPQRPEPTFP
210 220 230 240 250
LQGTLETQPA SDIIDIDYFE GLDSEGRGTD MGRFPGSPGT SENHPDTEGE
260 270 280 290 300
TPSWSLLDLY DDFTPFDESD FYPTTSFYDD LEEEEEEEED KDAVGGGDLE
310 320 330 340 350
DESDLLLPSQ KPGVGPGTGQ PTSRWHAVPP QHTLGMVPGG SISLRPRPGD
360 370 380 390 400
PGKDLATSEN GTECRVGFVR HNGSCRSVCD LFPSYCHNGG QCYLVENIGA
410 420 430 440 450
FCRCNTQDYI WHKGMRCESI ITDFQVMCVA VGSAALVLLL LFMMTVFFAK
460 470 480 490 500
KLYLLKTENT KLRRTNKFRT PSELHNDNFS LSTIAEGSHP NVRKLCDTPC
510 520 530 540 550
VSSPHARALA HCDNIVCQDD PSAPHKIQEA LKSRLKEEES FNIQNSMSPK
560 570
LEGGKGDQDD LEVNCLQNNL T
Length:571
Mass (Da):60,959
Last modified:March 1, 2001 - v1
Checksum:iCF42A0115F0CD11E
GO
Isoform 2 (identifier: Q9ERQ6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     492-518: Missing.

Show »
Length:544
Mass (Da):58,039
Checksum:i51C364613203A21E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti565C → F in AAC98537 (PubMed:7592931).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015765492 – 518Missing in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33553 mRNA. Translation: AAC98537.1.
AF292102 mRNA. Translation: AAG29500.1.
PIRiI55454.
RefSeqiNP_062157.1. NM_019284.1.
NP_598413.1. NM_133652.1. [Q9ERQ6-1]
UniGeneiRn.10146.

Genome annotation databases

GeneIDi50568.
KEGGirno:50568.
UCSCiRGD:2431. rat. [Q9ERQ6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCSPG5_RAT
AccessioniPrimary (citable) accession number: Q9ERQ6
Secondary accession number(s): Q62831
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2001
Last modified: October 25, 2017
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome