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Protein

Diacylglycerol O-acyltransferase 1

Gene

Dgat1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. In liver, plays a role in esterifying exogenous fatty acids to glycerol.By similarity

Catalytic activityi

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Acyl-CoA + retinol = CoA + retinyl ester.

Pathwayi: glycerolipid metabolism

This protein is involved in the pathway glycerolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway glycerolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei426By similarity1

GO - Molecular functioni

  • diacylglycerol binding Source: RGD
  • diacylglycerol O-acyltransferase activity Source: RGD
  • fatty acid binding Source: RGD
  • retinol O-fatty-acyltransferase activity Source: UniProtKB-EC
  • transferase activity, transferring acyl groups Source: RGD

GO - Biological processi

  • glycerolipid metabolic process Source: RGD
  • insulin secretion Source: RGD
  • ketone body metabolic process Source: RGD
  • positive regulation of fatty acid oxidation Source: RGD
  • positive regulation of hepatic stellate cell activation Source: RGD
  • positive regulation of triglyceride biosynthetic process Source: RGD
  • regulation of eating behavior Source: RGD
  • regulation of intestinal lipid absorption Source: RGD
  • regulation of vitamin A metabolic process Source: RGD
  • response to human chorionic gonadotropin Source: RGD
  • triglyceride biosynthetic process Source: RGD

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00230

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol O-acyltransferase 1 (EC:2.3.1.20)
Alternative name(s):
Acyl-CoA retinol O-fatty-acyltransferase (EC:2.3.1.76)
Short name:
ARAT
Short name:
Retinol O-fatty-acyltransferase
Diglyceride acyltransferase
Gene namesi
Name:Dgat1
Synonyms:Dgat
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628673 Dgat1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 96CytoplasmicSequence analysisAdd BLAST96
Transmembranei97 – 118HelicalSequence analysisAdd BLAST22
Topological domaini119 – 230LumenalSequence analysisAdd BLAST112
Transmembranei231 – 240HelicalSequence analysis10
Topological domaini241CytoplasmicSequence analysis1
Transmembranei242 – 250HelicalSequence analysis9
Topological domaini251 – 498LumenalSequence analysisAdd BLAST248

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL6129

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002076561 – 498Diacylglycerol O-acyltransferase 1Add BLAST498

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9ERM3
PeptideAtlasiQ9ERM3
PRIDEiQ9ERM3

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000035110

Chemistry databases

BindingDBiQ9ERM3

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 96Involved in homomerizationBy similarityAdd BLAST96

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0380 Eukaryota
COG5056 LUCA
HOVERGENiHBG051341
InParanoidiQ9ERM3
KOiK11155
PhylomeDBiQ9ERM3

Family and domain databases

InterProiView protein in InterPro
IPR027251 Diacylglycerol_acylTrfase1
IPR004299 MBOAT_fam
IPR014371 Oat_ACAT_DAG_ARE
PANTHERiPTHR10408 PTHR10408, 1 hit
PTHR10408:SF13 PTHR10408:SF13, 1 hit
PfamiView protein in Pfam
PF03062 MBOAT, 1 hit
PIRSFiPIRSF000439 Oat_ACAT_DAG_ARE, 1 hit
PIRSF500231 Oat_dag, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ERM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDRGGAGSS RRRRTGSRVS VQGGSGPKVE EDEVREAAVS PDLGAGGDAP
60 70 80 90 100
APAPAPAHTR DKDRQTSVGD GHWELRCHRL QDSLFSSDSG FSNYRGILNW
110 120 130 140 150
CVVMLILSNA RLSLENLIKY GILVDPIQVV SLFLKDPYSW PAPCLIIASN
160 170 180 190 200
IFIVATFQIE KRLSVGALTE QMGLLLHVVN LATIICFPAA VALLVESITP
210 220 230 240 250
VGSLFALASY SIIFLKLSSY RDVNLWCRQR RVKAKAVSAG KKVSGAAAQN
260 270 280 290 300
TVSYPDNLTY RDLYYFIFAP TLCYELNFPR SPRIRKRFLL RRVLEMLFFT
310 320 330 340 350
QLQVGLIQQW MVPTIQNSMK PFKDMDYSRI IERLLKLAVP NHLIWLIFFY
360 370 380 390 400
WLFHSCLNAV AELLQFGDRE FYRDWWNAES VTYFWQNWNI PVHKWCIRHF
410 420 430 440 450
YKPMLRLGSN KWMARTGVFW ASAFFHEYLV SIPLRMFRLW AFTAMMAQVP
460 470 480 490
LAWIVNRFFQ GNYGNAAVWV TLIIGQPVAV LMYVHDYYVL NYDAPVGA
Length:498
Mass (Da):56,870
Last modified:March 1, 2001 - v1
Checksum:i5B24DD4AEB87CB4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296131 mRNA Translation: AAG10084.1
RefSeqiNP_445889.1, NM_053437.1
UniGeneiRn.252

Genome annotation databases

GeneIDi84497
KEGGirno:84497
UCSCiRGD:628673 rat

Similar proteinsi

Entry informationi

Entry nameiDGAT1_RAT
AccessioniPrimary (citable) accession number: Q9ERM3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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