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Reviewed, UniProtKB/Swiss-Prot Q9ERM3 (DGAT1_RAT)

Last modified October 13, 2009. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Diacylglycerol O-acyltransferase 1
    EC=2.3.1.20
Alternative name(s):
    Diglyceride acyltransferase
Gene names
Name: Dgat1
Synonyms: Dgat
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly By similarity.

Catalytic activity

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.

Pathway

Lipid metabolism; glycerolipid metabolism.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 498498Diacylglycerol O-acyltransferase 1
PRO_0000207656

Regions

Transmembrane113 – 13321 Potential
Transmembrane139 – 15921 Potential
Transmembrane175 – 19521 Potential
Transmembrane198 – 21821 Potential
Transmembrane293 – 31321 Potential
Transmembrane343 – 36321 Potential
Transmembrane417 – 43721 Potential
Transmembrane439 – 45921 Potential
Transmembrane464 – 48421 Potential

Sites

Active site4261 Potential

Amino acid modifications

Modified residue201Phosphoserine By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9ERM3-1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 5B24DD4AEB87CB4D

FASTA49856,870
        10         20         30         40         50         60 
MGDRGGAGSS RRRRTGSRVS VQGGSGPKVE EDEVREAAVS PDLGAGGDAP APAPAPAHTR 

        70         80         90        100        110        120 
DKDRQTSVGD GHWELRCHRL QDSLFSSDSG FSNYRGILNW CVVMLILSNA RLSLENLIKY 

       130        140        150        160        170        180 
GILVDPIQVV SLFLKDPYSW PAPCLIIASN IFIVATFQIE KRLSVGALTE QMGLLLHVVN 

       190        200        210        220        230        240 
LATIICFPAA VALLVESITP VGSLFALASY SIIFLKLSSY RDVNLWCRQR RVKAKAVSAG 

       250        260        270        280        290        300 
KKVSGAAAQN TVSYPDNLTY RDLYYFIFAP TLCYELNFPR SPRIRKRFLL RRVLEMLFFT 

       310        320        330        340        350        360 
QLQVGLIQQW MVPTIQNSMK PFKDMDYSRI IERLLKLAVP NHLIWLIFFY WLFHSCLNAV 

       370        380        390        400        410        420 
AELLQFGDRE FYRDWWNAES VTYFWQNWNI PVHKWCIRHF YKPMLRLGSN KWMARTGVFW 

       430        440        450        460        470        480 
ASAFFHEYLV SIPLRMFRLW AFTAMMAQVP LAWIVNRFFQ GNYGNAAVWV TLIIGQPVAV 

       490 
LMYVHDYYVL NYDAPVGA

« Hide

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References

[1]"Cloning of a rat novel gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis."
Zhang Y., Yang Q., Basse P., Rice P.
Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Brown Norway.
Tissue: Brain.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF296131 mRNA. Translation: AAG10084.1.
IPIIPI00191170.
RefSeqNP_445889.1.
UniGeneRn.252

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ9ERM3.

Proteomic databases

PRIDEQ9ERM3.

Genome annotation databases

EnsemblENSRNOT00000039795; ENSRNOP00000035110; ENSRNOG00000028711; Rattus norvegicus. [Genome view]
GeneID84497.
KEGGrno:84497.
UCSCNM_053437. rat.

Organism-specific databases

CTD84497.
RGD628673. Dgat1.

Phylogenomic databases

HOVERGENQ9ERM3.

Enzyme and pathway databases

BRENDA2.3.1.20. 248.

Gene expression databases

ArrayExpressQ9ERM3.
GenevestigatorQ9ERM3.
GermOnlineENSRNOG00000028711. Rattus norvegicus.

Family and domain databases

InterProIPR004299. MBOAT_fam.
[Graphical view]
PfamPF03062. MBOAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio617075.

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Entry information

Entry nameDGAT1_RAT
AccessionPrimary (citable) accession number: Q9ERM3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: March 1, 2001
Last modified: October 13, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents