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Protein

Guanylate cyclase soluble subunit alpha-3

Gene

Gucy1a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.By similarity

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Has also activity with Mn2+ (in vitro).By similarity

Enzyme regulationi

Activated by nitric oxide in the presence of magnesium or manganese ions.By similarity

GO - Molecular functioni

GO - Biological processi

  • cGMP biosynthetic process Source: MGI
  • nitric oxide mediated signal transduction Source: Ensembl
  • positive regulation of cGMP biosynthetic process Source: MGI
  • regulation of blood pressure Source: MGI
  • relaxation of vascular smooth muscle Source: MGI
  • response to defense-related host nitric oxide production Source: MGI
  • response to herbicide Source: Ensembl
  • response to organic cyclic compound Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cGMP biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate cyclase soluble subunit alpha-3 (EC:4.6.1.2By similarity)
Short name:
GCS-alpha-3
Alternative name(s):
GCS-alpha-1
Soluble guanylate cyclase large subunit
Gene namesi
Name:Gucy1a3
Synonyms:Gucy1a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1926562. Gucy1a3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 691691Guanylate cyclase soluble subunit alpha-3PRO_0000074111Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei267 – 2671PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9ERL9.
MaxQBiQ9ERL9.
PaxDbiQ9ERL9.
PRIDEiQ9ERL9.

PTM databases

iPTMnetiQ9ERL9.
PhosphoSiteiQ9ERL9.

Expressioni

Gene expression databases

BgeeiQ9ERL9.
CleanExiMM_GUCY1A3.
ExpressionAtlasiQ9ERL9. baseline and differential.
GenevisibleiQ9ERL9. MM.

Interactioni

Subunit structurei

The active enzyme is formed by a heterodimer of an alpha and a beta subunit. Heterodimer with GUCY1B3.By similarity

Protein-protein interaction databases

IntActiQ9ERL9. 1 interaction.
MINTiMINT-4095867.
STRINGi10090.ENSMUSP00000048918.

Structurei

3D structure databases

ProteinModelPortaliQ9ERL9.
SMRiQ9ERL9. Positions 281-387, 406-466, 471-662.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini480 – 607128Guanylate cyclasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4171. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000220903.
HOVERGENiHBG106603.
InParanoidiQ9ERL9.
KOiK12318.
OMAiSEIPGIC.
OrthoDBiEOG7VX8Z6.
TreeFamiTF351403.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR011644. Heme_NO-bd.
IPR011645. HNOB_dom_associated.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 1 hit.
PF07700. HNOB. 1 hit.
PF07701. HNOBA. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF111126. SSF111126. 1 hit.
SSF55073. SSF55073. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ERL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFCRKFKDLK ITGECPFSLL APGQVPKEPT EEVAGGSEGC QATLPICQYF
60 70 80 90 100
PEKNAEGSLP QRKTSRNRVY LHTLAESICK LIFPECERLN LALQRTLAKH
110 120 130 140 150
KIEENRKSSE KEDLEKIIAE EAIAAGAPVE ALKDSLGEEL FKICYEEDEH
160 170 180 190 200
ILGVVGGTLK DFLNSFSTLL KQSSHCQEAE RRGRLEDASI LCLDKDQDFL
210 220 230 240 250
NVYYFFPKRT TALLLPGIIK AAARILYESH VEVSLMPPCF RSDCTEFVNQ
260 270 280 290 300
PYLLYSVHVK STKPSLSPGK PQSSLVIPAS LFCKTFPFHF MLDRDLAILQ
310 320 330 340 350
LGNGIRRLVN KRDFQGKPNF EEFFEILTPK INQTFSGIMT MLNMQFVIRV
360 370 380 390 400
RRWDNSVKKS SRVMDLKGQM IYIVESSAIL FLGSPCVDRL EDFTGRGLYL
410 420 430 440 450
SDIPIHNALR DVVLIGEQAR AQDGLKKRLG KLKATLEHAH QALEEEKKRT
460 470 480 490 500
VDLLCSIFPS EVAQQLWQGQ IVQAKKFSEV TMLFSDIVGF TAICSQCSPL
510 520 530 540 550
QVITMLNALY TRFDQQCGEL DVYKVETIGD AYCVAGGLHR ESDTHAVQIA
560 570 580 590 600
LMALKMMELS NEVMSPHGEP IKMRIGLHSG SVFAGVVGVK MPRYCLFGNN
610 620 630 640 650
VTLANKFESC SVPRKINVSP TTYRLLKDCP GFVFTPRSRE ELPPNFPSDI
660 670 680 690
PGICHFLDAY HHQGPNSKPW FQDKDVEDGN ANFLGKASGI D
Length:691
Mass (Da):77,588
Last modified:July 27, 2011 - v2
Checksum:iFF8DD0C3CB0BBD7F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti690 – 6901I → V in AAG17446 (PubMed:10984516).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF297082 mRNA. Translation: AAG17446.1.
AK004815 mRNA. Translation: BAB23586.1.
AK028558 mRNA. Translation: BAC26009.1.
BC057327 mRNA. Translation: AAH57327.1.
BC094310 mRNA. Translation: AAH94310.1.
CCDSiCCDS17429.1.
RefSeqiNP_068696.2. NM_021896.5.
XP_006501906.1. XM_006501843.2.
XP_011238486.1. XM_011240184.1.
UniGeneiMm.143831.

Genome annotation databases

EnsembliENSMUST00000048976; ENSMUSP00000048918; ENSMUSG00000033910.
ENSMUST00000193924; ENSMUSP00000142138; ENSMUSG00000033910.
GeneIDi60596.
KEGGimmu:60596.
UCSCiuc008pou.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF297082 mRNA. Translation: AAG17446.1.
AK004815 mRNA. Translation: BAB23586.1.
AK028558 mRNA. Translation: BAC26009.1.
BC057327 mRNA. Translation: AAH57327.1.
BC094310 mRNA. Translation: AAH94310.1.
CCDSiCCDS17429.1.
RefSeqiNP_068696.2. NM_021896.5.
XP_006501906.1. XM_006501843.2.
XP_011238486.1. XM_011240184.1.
UniGeneiMm.143831.

3D structure databases

ProteinModelPortaliQ9ERL9.
SMRiQ9ERL9. Positions 281-387, 406-466, 471-662.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9ERL9. 1 interaction.
MINTiMINT-4095867.
STRINGi10090.ENSMUSP00000048918.

PTM databases

iPTMnetiQ9ERL9.
PhosphoSiteiQ9ERL9.

Proteomic databases

EPDiQ9ERL9.
MaxQBiQ9ERL9.
PaxDbiQ9ERL9.
PRIDEiQ9ERL9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048976; ENSMUSP00000048918; ENSMUSG00000033910.
ENSMUST00000193924; ENSMUSP00000142138; ENSMUSG00000033910.
GeneIDi60596.
KEGGimmu:60596.
UCSCiuc008pou.2. mouse.

Organism-specific databases

CTDi2982.
MGIiMGI:1926562. Gucy1a3.

Phylogenomic databases

eggNOGiKOG4171. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000220903.
HOVERGENiHBG106603.
InParanoidiQ9ERL9.
KOiK12318.
OMAiSEIPGIC.
OrthoDBiEOG7VX8Z6.
TreeFamiTF351403.

Miscellaneous databases

NextBioi315023.
PROiQ9ERL9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9ERL9.
CleanExiMM_GUCY1A3.
ExpressionAtlasiQ9ERL9. baseline and differential.
GenevisibleiQ9ERL9. MM.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR011644. Heme_NO-bd.
IPR011645. HNOB_dom_associated.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 1 hit.
PF07700. HNOB. 1 hit.
PF07701. HNOBA. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF111126. SSF111126. 1 hit.
SSF55073. SSF55073. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization of alpha 1 and beta 1 subunits of the mammalian soluble guanylyl cyclase genes."
    Sharina I.G., Krumenacker J.S., Martin E., Murad F.
    Proc. Natl. Acad. Sci. U.S.A. 97:10878-10883(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Lung.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-267, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Lung and Spleen.

Entry informationi

Entry nameiGCYA3_MOUSE
AccessioniPrimary (citable) accession number: Q9ERL9
Secondary accession number(s): Q6GTG0, Q9DBQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.