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Q9ERI2

- RB27A_MOUSE

UniProt

Q9ERI2 - RB27A_MOUSE

Protein

Ras-related protein Rab-27A

Gene

Rab27a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 249GTP
    Nucleotide bindingi74 – 785GTP
    Nucleotide bindingi133 – 1364GTP
    Nucleotide bindingi163 – 1653GTP

    GO - Molecular functioni

    1. GDP binding Source: UniProtKB
    2. GTPase activity Source: UniProtKB
    3. GTP binding Source: UniProtKB
    4. myosin V binding Source: MGI
    5. protein binding Source: UniProtKB

    GO - Biological processi

    1. blood coagulation Source: MGI
    2. cytotoxic T cell degranulation Source: MGI
    3. GTP catabolic process Source: GOC
    4. melanocyte differentiation Source: MGI
    5. melanosome localization Source: MGI
    6. melanosome transport Source: MGI
    7. natural killer cell degranulation Source: MGI
    8. pigmentation Source: MGI
    9. pigment granule localization Source: MGI
    10. pigment granule transport Source: MGI
    11. protein targeting Source: MGI
    12. small GTPase mediated signal transduction Source: InterPro
    13. vesicle-mediated transport Source: MGI

    Keywords - Biological processi

    Exocytosis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_207837. Insulin processing.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-27A
    Gene namesi
    Name:Rab27a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1861441. Rab27a.

    Subcellular locationi

    Membrane; Lipid-anchor. Melanosome By similarity. Late endosome By similarity. Lysosome By similarity
    Note: Localizes to endosomal exocytic vesicles.By similarity

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. Golgi apparatus Source: MGI
    3. intracellular Source: MGI
    4. late endosome Source: UniProtKB-SubCell
    5. lysosome Source: UniProtKB-SubCell
    6. melanosome Source: UniProtKB
    7. membrane Source: UniProtKB-SubCell
    8. photoreceptor outer segment Source: UniProtKB
    9. secretory granule Source: MGI

    Keywords - Cellular componenti

    Endosome, Lysosome, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi78 – 781Q → L: Loss of GTPase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 221220Ras-related protein Rab-27APRO_0000121222Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Disulfide bondi123 ↔ 188By similarity
    Lipidationi219 – 2191S-geranylgeranyl cysteineBy similarity
    Modified residuei221 – 2211Cysteine methyl esterBy similarity
    Lipidationi221 – 2211S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Acetylation, Disulfide bond, Lipoprotein, Methylation, Prenylation

    Proteomic databases

    MaxQBiQ9ERI2.
    PaxDbiQ9ERI2.
    PRIDEiQ9ERI2.

    PTM databases

    PhosphoSiteiQ9ERI2.

    Expressioni

    Tissue specificityi

    Detected in melanocytes. Expressed abundantly in the stomach and is predominantly localized at the apical region of gastric-surface mucus cells. Also expressed in the thymus and lung.1 Publication

    Gene expression databases

    ArrayExpressiQ9ERI2.
    BgeeiQ9ERI2.
    CleanExiMM_RAB27A.
    GenevestigatoriQ9ERI2.

    Interactioni

    Subunit structurei

    Binds SYTL1, SYTL2, SLAC2B, MYRIP, SYTL3, SYTL4, SYTL5 and MLPH. Interacts with UNC13D. Interacts with RPH3A and RPH3A.8 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Rph3aP477082EBI-398172,EBI-398376
    SYTL5Q8TDW52EBI-398172,EBI-2939487From a different organism.

    Protein-protein interaction databases

    BioGridi198222. 4 interactions.
    DIPiDIP-31054N.
    IntActiQ9ERI2. 11 interactions.

    Structurei

    Secondary structure

    1
    221
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 159
    Helixi22 – 3110
    Beta strandi43 – 5311
    Beta strandi66 – 7510
    Helixi79 – 813
    Helixi82 – 876
    Turni88 – 914
    Beta strandi93 – 1008
    Helixi104 – 1085
    Helixi110 – 12011
    Beta strandi121 – 1255
    Beta strandi128 – 1336
    Helixi138 – 1403
    Helixi145 – 15511
    Beta strandi159 – 1613
    Turni164 – 1663
    Helixi170 – 18718

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3BC1X-ray1.80A/E1-193[»]
    ProteinModelPortaliQ9ERI2.
    SMRiQ9ERI2. Positions 4-187.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9ERI2.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi38 – 469Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00740000114962.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiQ9ERI2.
    KOiK07885.
    OMAiDQRAVKE.
    OrthoDBiEOG7FFMSC.
    PhylomeDBiQ9ERI2.
    TreeFamiTF312895.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9ERI2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR    50
    VVYRANGPDG AVGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD 100
    LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RAVKEEEARE 150
    LAEKYGIPYF ETSAANGTNI SHAIEMLLDL IMKRMERCVD KSWIPEGVVR 200
    SNGHTSADQL SEEKEKGLCG C 221
    Length:221
    Mass (Da):25,017
    Last modified:March 1, 2001 - v1
    Checksum:iC6857EBDD7F38137
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF304376 mRNA. Translation: AAG24638.1.
    AB046693 mRNA. Translation: BAB62313.1.
    AK010373 mRNA. Translation: BAB26891.1.
    BC008173 mRNA. Translation: AAH08173.1.
    BC009656 mRNA. Translation: AAH09656.1.
    CCDSiCCDS23335.1.
    RefSeqiNP_076124.1. NM_023635.5.
    XP_006510843.1. XM_006510780.1.
    XP_006510844.1. XM_006510781.1.
    XP_006510845.1. XM_006510782.1.
    UniGeneiMm.34867.

    Genome annotation databases

    EnsembliENSMUST00000034722; ENSMUSP00000034722; ENSMUSG00000032202.
    ENSMUST00000184146; ENSMUSP00000139310; ENSMUSG00000032202.
    GeneIDi11891.
    KEGGimmu:11891.
    UCSCiuc009qqv.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF304376 mRNA. Translation: AAG24638.1 .
    AB046693 mRNA. Translation: BAB62313.1 .
    AK010373 mRNA. Translation: BAB26891.1 .
    BC008173 mRNA. Translation: AAH08173.1 .
    BC009656 mRNA. Translation: AAH09656.1 .
    CCDSi CCDS23335.1.
    RefSeqi NP_076124.1. NM_023635.5.
    XP_006510843.1. XM_006510780.1.
    XP_006510844.1. XM_006510781.1.
    XP_006510845.1. XM_006510782.1.
    UniGenei Mm.34867.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3BC1 X-ray 1.80 A/E 1-193 [» ]
    ProteinModelPortali Q9ERI2.
    SMRi Q9ERI2. Positions 4-187.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198222. 4 interactions.
    DIPi DIP-31054N.
    IntActi Q9ERI2. 11 interactions.

    PTM databases

    PhosphoSitei Q9ERI2.

    Proteomic databases

    MaxQBi Q9ERI2.
    PaxDbi Q9ERI2.
    PRIDEi Q9ERI2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034722 ; ENSMUSP00000034722 ; ENSMUSG00000032202 .
    ENSMUST00000184146 ; ENSMUSP00000139310 ; ENSMUSG00000032202 .
    GeneIDi 11891.
    KEGGi mmu:11891.
    UCSCi uc009qqv.1. mouse.

    Organism-specific databases

    CTDi 5873.
    MGIi MGI:1861441. Rab27a.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00740000114962.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi Q9ERI2.
    KOi K07885.
    OMAi DQRAVKE.
    OrthoDBi EOG7FFMSC.
    PhylomeDBi Q9ERI2.
    TreeFami TF312895.

    Enzyme and pathway databases

    Reactomei REACT_207837. Insulin processing.

    Miscellaneous databases

    ChiTaRSi RAB27A. mouse.
    EvolutionaryTracei Q9ERI2.
    NextBioi 279933.
    PROi Q9ERI2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9ERI2.
    Bgeei Q9ERI2.
    CleanExi MM_RAB27A.
    Genevestigatori Q9ERI2.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H/HeSn.
    2. Izumi T.
      Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryonic stem cell.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Synaptotagmin-like protein 5: a novel Rab27A effector with C-terminal tandem C2 domains."
      Kuroda T.S., Fukuda M., Ariga H., Mikoshiba K.
      Biochem. Biophys. Res. Commun. 293:899-906(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SYTL5.
    6. "The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain."
      Kuroda T.S., Fukuda M., Ariga H., Mikoshiba K.
      J. Biol. Chem. 277:9212-9218(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SYTL1; SYTL2; SYTL3; SYTL4; SLAC2B AND MYRIP.
    7. Cited for: INTERACTION WITH MLPH.
    8. "Distinct Rab binding specificity of Rim1, Rim2, rabphilin, and Noc2. Identification of a critical determinant of Rab3A/Rab27A recognition by Rim2."
      Fukuda M.
      J. Biol. Chem. 278:15373-15380(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RPH3A AND RPH3AL.
    9. "Decreased basal mucus secretion by Slp2-a-deficient gastric surface mucous cells."
      Saegusa C., Tanaka T., Tani S., Itohara S., Mikoshiba K., Fukuda M.
      Genes Cells 11:623-631(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INTERACTION WITH SYTL2.
    10. "Doc2 alpha and Munc13-4 regulate Ca(2+) -dependent secretory lysosome exocytosis in mast cells."
      Higashio H., Nishimura N., Ishizaki H., Miyoshi J., Orita S., Sakane A., Sasaki T.
      J. Immunol. 180:4774-4784(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UNC13D.
    11. "Slp1 and Slp2-a localize to the plasma membrane of CTL and contribute to secretion from the immunological synapse."
      Holt O., Kanno E., Bossi G., Booth S., Daniele T., Santoro A., Arico M., Saegusa C., Fukuda M., Griffiths G.M.
      Traffic 9:446-457(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SYTL1 AND SYTL2.
    12. "Elucidation of Rab27 recruitment by its effectors: structure of Rab27a bound to Exophilin4/Slp2-a."
      Chavas L.M., Ihara K., Kawasaki M., Torii S., Uejima T., Kato R., Izumi T., Wakatsuki S.
      Structure 16:1468-1477(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 1-193 IN COMPLEX WITH GTP ANALOG, INTERACTION WITH SYTL2, MUTAGENESIS OF GLN-78.

    Entry informationi

    Entry nameiRB27A_MOUSE
    AccessioniPrimary (citable) accession number: Q9ERI2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2002
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3