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Protein

Ras-related protein Rab-27A

Gene

Rab27a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 24GTP9
Nucleotide bindingi74 – 78GTP5
Nucleotide bindingi133 – 136GTP4
Nucleotide bindingi163 – 165GTP3

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • myosin V binding Source: MGI

GO - Biological processi

  • antigen processing and presentation Source: MGI
  • blood coagulation Source: MGI
  • complement-dependent cytotoxicity Source: MGI
  • cytotoxic T cell degranulation Source: MGI
  • exocytosis Source: MGI
  • exosomal secretion Source: MGI
  • melanocyte differentiation Source: MGI
  • melanosome localization Source: MGI
  • melanosome transport Source: MGI
  • multivesicular body organization Source: MGI
  • multivesicular body sorting pathway Source: MGI
  • natural killer cell degranulation Source: MGI
  • pigmentation Source: MGI
  • pigment granule localization Source: MGI
  • pigment granule transport Source: MGI
  • positive regulation of constitutive secretory pathway Source: MGI
  • positive regulation of exocytosis Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of phagocytosis Source: MGI
  • positive regulation of reactive oxygen species biosynthetic process Source: MGI
  • positive regulation of regulated secretory pathway Source: MGI
  • protein targeting Source: MGI
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle-mediated transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-264876. Insulin processing.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-27A
Gene namesi
Name:Rab27a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1861441. Rab27a.

Subcellular locationi

  • Membrane By similarity; Lipid-anchor By similarity
  • Melanosome By similarity
  • Late endosome By similarity
  • Lysosome By similarity

  • Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles.By similarity

GO - Cellular componenti

  • apical plasma membrane Source: Ensembl
  • cytosol Source: Reactome
  • dendrite Source: MGI
  • exocytic vesicle Source: MGI
  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • intracellular Source: MGI
  • late endosome Source: MGI
  • lysosome Source: MGI
  • melanosome Source: UniProtKB
  • multivesicular body membrane Source: MGI
  • photoreceptor outer segment Source: UniProtKB
  • secretory granule Source: MGI
  • secretory granule membrane Source: Ensembl
  • Weibel-Palade body Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi78Q → L: Loss of GTPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001212222 – 221Ras-related protein Rab-27AAdd BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Disulfide bondi123 ↔ 188By similarity
Lipidationi219S-geranylgeranyl cysteineBy similarity1
Modified residuei221Cysteine methyl esterBy similarity1
Lipidationi221S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiQ9ERI2.
MaxQBiQ9ERI2.
PaxDbiQ9ERI2.
PRIDEiQ9ERI2.

PTM databases

iPTMnetiQ9ERI2.
PhosphoSitePlusiQ9ERI2.

Expressioni

Tissue specificityi

Detected in melanocytes. Expressed abundantly in the stomach and is predominantly localized at the apical region of gastric-surface mucus cells. Also expressed in the thymus and lung.1 Publication

Gene expression databases

BgeeiENSMUSG00000032202.
CleanExiMM_RAB27A.
ExpressionAtlasiQ9ERI2. baseline and differential.
GenevisibleiQ9ERI2. MM.

Interactioni

Subunit structurei

Binds SYTL1, SYTL2, SLAC2B, MYRIP, SYTL3, SYTL4, SYTL5 and MLPH. Interacts with UNC13D. Interacts with RPH3A and RPH3A. Does not interact with the BLOC-3 complex (heterodimer of HPS1 and HPS4) (By similarity).By similarity8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rph3aP477082EBI-398172,EBI-398376
SYTL5Q8TDW52EBI-398172,EBI-2939487From a different organism.

GO - Molecular functioni

  • myosin V binding Source: MGI

Protein-protein interaction databases

BioGridi198222. 6 interactors.
DIPiDIP-31054N.
IntActiQ9ERI2. 13 interactors.
STRINGi10090.ENSMUSP00000034722.

Structurei

Secondary structure

1221
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 15Combined sources9
Helixi22 – 31Combined sources10
Beta strandi43 – 53Combined sources11
Beta strandi66 – 75Combined sources10
Helixi79 – 81Combined sources3
Helixi82 – 87Combined sources6
Turni88 – 91Combined sources4
Beta strandi93 – 100Combined sources8
Helixi104 – 108Combined sources5
Helixi110 – 120Combined sources11
Beta strandi121 – 125Combined sources5
Beta strandi128 – 133Combined sources6
Helixi138 – 140Combined sources3
Helixi145 – 155Combined sources11
Beta strandi159 – 161Combined sources3
Turni164 – 166Combined sources3
Helixi170 – 187Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BC1X-ray1.80A/E1-193[»]
ProteinModelPortaliQ9ERI2.
SMRiQ9ERI2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ERI2.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi38 – 46Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0081. Eukaryota.
ENOG410XQ3T. LUCA.
GeneTreeiENSGT00860000133677.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ9ERI2.
KOiK07885.
OMAiTSFLHRY.
OrthoDBiEOG091G0I51.
PhylomeDBiQ9ERI2.
TreeFamiTF312895.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ERI2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR
60 70 80 90 100
VVYRANGPDG AVGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD
110 120 130 140 150
LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RAVKEEEARE
160 170 180 190 200
LAEKYGIPYF ETSAANGTNI SHAIEMLLDL IMKRMERCVD KSWIPEGVVR
210 220
SNGHTSADQL SEEKEKGLCG C
Length:221
Mass (Da):25,017
Last modified:March 1, 2001 - v1
Checksum:iC6857EBDD7F38137
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF304376 mRNA. Translation: AAG24638.1.
AB046693 mRNA. Translation: BAB62313.1.
AK010373 mRNA. Translation: BAB26891.1.
BC008173 mRNA. Translation: AAH08173.1.
BC009656 mRNA. Translation: AAH09656.1.
CCDSiCCDS23335.1.
RefSeqiNP_001288159.1. NM_001301230.1.
NP_001288161.1. NM_001301232.1.
NP_076124.1. NM_023635.6.
XP_006510843.1. XM_006510780.3.
UniGeneiMm.34867.
Mm.490032.

Genome annotation databases

EnsembliENSMUST00000034722; ENSMUSP00000034722; ENSMUSG00000032202.
ENSMUST00000184146; ENSMUSP00000139310; ENSMUSG00000032202.
GeneIDi11891.
KEGGimmu:11891.
UCSCiuc009qqv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF304376 mRNA. Translation: AAG24638.1.
AB046693 mRNA. Translation: BAB62313.1.
AK010373 mRNA. Translation: BAB26891.1.
BC008173 mRNA. Translation: AAH08173.1.
BC009656 mRNA. Translation: AAH09656.1.
CCDSiCCDS23335.1.
RefSeqiNP_001288159.1. NM_001301230.1.
NP_001288161.1. NM_001301232.1.
NP_076124.1. NM_023635.6.
XP_006510843.1. XM_006510780.3.
UniGeneiMm.34867.
Mm.490032.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BC1X-ray1.80A/E1-193[»]
ProteinModelPortaliQ9ERI2.
SMRiQ9ERI2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198222. 6 interactors.
DIPiDIP-31054N.
IntActiQ9ERI2. 13 interactors.
STRINGi10090.ENSMUSP00000034722.

PTM databases

iPTMnetiQ9ERI2.
PhosphoSitePlusiQ9ERI2.

Proteomic databases

EPDiQ9ERI2.
MaxQBiQ9ERI2.
PaxDbiQ9ERI2.
PRIDEiQ9ERI2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034722; ENSMUSP00000034722; ENSMUSG00000032202.
ENSMUST00000184146; ENSMUSP00000139310; ENSMUSG00000032202.
GeneIDi11891.
KEGGimmu:11891.
UCSCiuc009qqv.2. mouse.

Organism-specific databases

CTDi5873.
MGIiMGI:1861441. Rab27a.

Phylogenomic databases

eggNOGiKOG0081. Eukaryota.
ENOG410XQ3T. LUCA.
GeneTreeiENSGT00860000133677.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ9ERI2.
KOiK07885.
OMAiTSFLHRY.
OrthoDBiEOG091G0I51.
PhylomeDBiQ9ERI2.
TreeFamiTF312895.

Enzyme and pathway databases

ReactomeiR-MMU-264876. Insulin processing.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

ChiTaRSiRab27a. mouse.
EvolutionaryTraceiQ9ERI2.
PROiQ9ERI2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032202.
CleanExiMM_RAB27A.
ExpressionAtlasiQ9ERI2. baseline and differential.
GenevisibleiQ9ERI2. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRB27A_MOUSE
AccessioniPrimary (citable) accession number: Q9ERI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.