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Protein

Solute carrier family 28 member 3

Gene

Slc28a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Sodium-dependent, pyrimidine- and purine-selective. Involved in the homeostasis of endogenous nucleosides. Exhibits the transport characteristics of the nucleoside transport system cib or N3 subtype (N3/cib) (with marked transport of both thymidine and inosine). Employs a 2:1 sodium/nucleoside ratio. Also able to transport gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine (By similarity).By similarity

GO - Molecular functioni

  • purine-specific nucleoside:sodium symporter activity Source: MGI
  • pyrimidine- and adenine-specific:sodium symporter activity Source: MGI

GO - Biological processi

  • purine nucleoside transmembrane transport Source: MGI
  • pyrimidine nucleoside transport Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-83936. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Protein family/group databases

TCDBi2.A.41.2.6. the concentrative nucleoside transporter (cnt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 28 member 3
Alternative name(s):
Concentrative Na(+)-nucleoside cotransporter 3
Short name:
CNT 3
Short name:
mCNT3
Gene namesi
Name:Slc28a3
Synonyms:Cnt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2137361. Slc28a3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 119CytoplasmicSequence analysisAdd BLAST119
Transmembranei120 – 140HelicalSequence analysisAdd BLAST21
Topological domaini141 – 142ExtracellularSequence analysis2
Transmembranei143 – 163HelicalSequence analysisAdd BLAST21
Topological domaini164 – 185CytoplasmicSequence analysisAdd BLAST22
Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
Topological domaini207 – 215ExtracellularSequence analysis9
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Topological domaini237 – 238CytoplasmicSequence analysis2
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Topological domaini260 – 302ExtracellularSequence analysisAdd BLAST43
Transmembranei303 – 323HelicalSequence analysisAdd BLAST21
Topological domaini324 – 329CytoplasmicSequence analysis6
Transmembranei330 – 350HelicalSequence analysisAdd BLAST21
Topological domaini351 – 377ExtracellularSequence analysisAdd BLAST27
Transmembranei378 – 398HelicalSequence analysisAdd BLAST21
Topological domaini399 – 403CytoplasmicSequence analysis5
Transmembranei404 – 424HelicalSequence analysisAdd BLAST21
Topological domaini425 – 458ExtracellularSequence analysisAdd BLAST34
Transmembranei459 – 479HelicalSequence analysisAdd BLAST21
Topological domaini480 – 493CytoplasmicSequence analysisAdd BLAST14
Transmembranei494 – 514HelicalSequence analysisAdd BLAST21
Topological domaini515 – 573ExtracellularSequence analysisAdd BLAST59
Transmembranei574 – 594HelicalSequence analysisAdd BLAST21
Topological domaini595 – 608CytoplasmicSequence analysisAdd BLAST14
Transmembranei609 – 629HelicalSequence analysisAdd BLAST21
Topological domaini630 – 703ExtracellularSequence analysisAdd BLAST74

GO - Cellular componenti

  • integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241471 – 703Solute carrier family 28 member 3Add BLAST703

Proteomic databases

PaxDbiQ9ERH8.
PRIDEiQ9ERH8.

PTM databases

PhosphoSitePlusiQ9ERH8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021553.
ExpressionAtlasiQ9ERH8. baseline and differential.
GenevisibleiQ9ERH8. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022036.

Structurei

3D structure databases

ProteinModelPortaliQ9ERH8.
SMRiQ9ERH8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3747. Eukaryota.
COG1972. LUCA.
GeneTreeiENSGT00390000016025.
HOGENOMiHOG000267658.
HOVERGENiHBG054073.
InParanoidiQ9ERH8.
KOiK11536.
OMAiGCCTLLN.
OrthoDBiEOG091G05D5.
PhylomeDBiQ9ERH8.
TreeFamiTF314131.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR030211. CNT3.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Gate_dom.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PTHR10590:SF18. PTHR10590:SF18. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ERH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRADPGKNS EPSESKMSLE LRPTAPSDLG RSNEAFQDED LERQNTPGNS
60 70 80 90 100
TVRNRVVQSG EQGHAKQDDR QITIEQEPLG NKEDPEDDSE DEHQKGFLER
110 120 130 140 150
KYDTICEFCR KHRVVLRSTI WAVLLTGFLA LVIAACAINF HRALPLFVIT
160 170 180 190 200
LVTIFFVIWD HLMAKYEQRI DDFLSPGRRL LDRHWFWLKW VVWSSLILAI
210 220 230 240 250
ILWLSLDTAK LGQQNLVSFG GLIMYLILLF LFSKHPTRVY WRPVFWGIGL
260 270 280 290 300
QFLLGLLILR TRPGFVAFDW MGRQVQTFLG YTDTGARFVF GEKYTDHFFA
310 320 330 340 350
FKILPIVVFF STVMSMLYYL GLMQWIIRKV GWLMLVTMGS SPIESVVAAG
360 370 380 390 400
NIFIGQTESP LLVQPYLPHV TKSELHTIMT AGFATIAGSV LGAYISFGVS
410 420 430 440 450
STHLLTASVM SAPAALAVAK LFWPETEKPK ITLKSAMKME NGDSRNLLEA
460 470 480 490 500
ASQGASSSIP LVANIAANLI AFLALLSFVN SALSWFGSMF NYPELSFELI
510 520 530 540 550
CSYIFMPFSF MMGVDWQDSF MVAKLIGYKT FFNEFVAYDH LSKLINLRKA
560 570 580 590 600
AGPKFVNGVQ QYMSIRSETI ATYALCGFAN FGSLGIVIGG LTSIAPSRKR
610 620 630 640 650
DIASGAMRAL IAGTIACFMT ACIAGILSDT PVDINCHHVL ENGRVLSNTT
660 670 680 690 700
EVVSCCQNLF NSTVAKGPND VVPGGNFSLY ALKSCCNLLK PPTLNCNWIP

NKL
Length:703
Mass (Da):78,310
Last modified:March 1, 2001 - v1
Checksum:i96D427BF15851C44
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69D → N in BAE26226 (PubMed:16141072).Curated1
Sequence conflicti139N → S in AAH10472 (PubMed:15489334).Curated1
Sequence conflicti139N → S in AAH13783 (PubMed:15489334).Curated1
Sequence conflicti171D → N in BAE26226 (PubMed:16141072).Curated1
Sequence conflicti540H → P in BAC35145 (PubMed:16141072).Curated1
Sequence conflicti666K → R in AAH10472 (PubMed:15489334).Curated1
Sequence conflicti666K → R in AAH13783 (PubMed:15489334).Curated1
Sequence conflicti697N → D in BAE26226 (PubMed:16141072).Curated1
Sequence conflicti697N → D in AAH10472 (PubMed:15489334).Curated1
Sequence conflicti697N → D in AAH13783 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305211 mRNA. Translation: AAG22552.1.
AK052784 mRNA. Translation: BAC35145.1.
AK145077 mRNA. Translation: BAE26226.1.
BC010472 mRNA. Translation: AAH10472.1.
BC013783 mRNA. Translation: AAH13783.1.
CCDSiCCDS26572.1.
RefSeqiNP_071712.3. NM_022317.3.
UniGeneiMm.18188.

Genome annotation databases

EnsembliENSMUST00000022036; ENSMUSP00000022036; ENSMUSG00000021553.
GeneIDi114304.
KEGGimmu:114304.
UCSCiuc007qud.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305211 mRNA. Translation: AAG22552.1.
AK052784 mRNA. Translation: BAC35145.1.
AK145077 mRNA. Translation: BAE26226.1.
BC010472 mRNA. Translation: AAH10472.1.
BC013783 mRNA. Translation: AAH13783.1.
CCDSiCCDS26572.1.
RefSeqiNP_071712.3. NM_022317.3.
UniGeneiMm.18188.

3D structure databases

ProteinModelPortaliQ9ERH8.
SMRiQ9ERH8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022036.

Protein family/group databases

TCDBi2.A.41.2.6. the concentrative nucleoside transporter (cnt) family.

PTM databases

PhosphoSitePlusiQ9ERH8.

Proteomic databases

PaxDbiQ9ERH8.
PRIDEiQ9ERH8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022036; ENSMUSP00000022036; ENSMUSG00000021553.
GeneIDi114304.
KEGGimmu:114304.
UCSCiuc007qud.1. mouse.

Organism-specific databases

CTDi64078.
MGIiMGI:2137361. Slc28a3.

Phylogenomic databases

eggNOGiKOG3747. Eukaryota.
COG1972. LUCA.
GeneTreeiENSGT00390000016025.
HOGENOMiHOG000267658.
HOVERGENiHBG054073.
InParanoidiQ9ERH8.
KOiK11536.
OMAiGCCTLLN.
OrthoDBiEOG091G05D5.
PhylomeDBiQ9ERH8.
TreeFamiTF314131.

Enzyme and pathway databases

ReactomeiR-MMU-83936. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Miscellaneous databases

PROiQ9ERH8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021553.
ExpressionAtlasiQ9ERH8. baseline and differential.
GenevisibleiQ9ERH8. MM.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR030211. CNT3.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Gate_dom.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PTHR10590:SF18. PTHR10590:SF18. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS28A3_MOUSE
AccessioniPrimary (citable) accession number: Q9ERH8
Secondary accession number(s): Q3UM72, Q8BWE2, Q91VD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.