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Q9ERG0

- LIMA1_MOUSE

UniProt

Q9ERG0 - LIMA1_MOUSE

Protein

LIM domain and actin-binding protein 1

Gene

Lima1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments By similarity.By similarity

    GO - Molecular functioni

    1. actin filament binding Source: UniProtKB
    2. actin monomer binding Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. actin filament bundle assembly Source: UniProtKB
    2. negative regulation of actin filament depolymerization Source: UniProtKB
    3. ruffle organization Source: UniProtKB

    Keywords - Ligandi

    Actin-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    LIM domain and actin-binding protein 1
    Alternative name(s):
    Epithelial protein lost in neoplasm
    Short name:
    mEPLIN
    Gene namesi
    Name:Lima1
    Synonyms:D15Ertd366e, Eplin
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1920992. Lima1.

    Subcellular locationi

    Cytoplasm. Cytoplasmcytoskeleton
    Note: This cytoskeletal protein colocalizes with actin stress fibers.

    GO - Cellular componenti

    1. actin cytoskeleton Source: MGI
    2. cytoplasm Source: UniProtKB-SubCell
    3. focal adhesion Source: UniProtKB
    4. plasma membrane Source: Ensembl
    5. stress fiber Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 753753LIM domain and actin-binding protein 1PRO_0000075731Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei15 – 151PhosphoserineBy similarity
    Modified residuei132 – 1321PhosphoserineBy similarity
    Modified residuei225 – 2251Phosphoserine2 Publications
    Modified residuei230 – 2301Phosphoserine1 Publication
    Modified residuei263 – 2631PhosphoserineBy similarity
    Modified residuei348 – 3481PhosphoserineBy similarity
    Modified residuei360 – 3601Phosphoserine1 Publication
    Modified residuei367 – 3671PhosphoserineBy similarity
    Modified residuei372 – 3721PhosphoserineBy similarity
    Modified residuei437 – 4371N6-succinyllysine1 Publication
    Modified residuei485 – 4851Phosphoserine1 Publication
    Modified residuei488 – 4881Phosphoserine4 Publications
    Modified residuei602 – 6021PhosphoserineBy similarity
    Modified residuei607 – 6071PhosphoserineBy similarity
    Modified residuei615 – 6151PhosphoserineBy similarity
    Modified residuei692 – 6921PhosphoserineBy similarity
    Modified residuei720 – 7201PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9ERG0.
    PaxDbiQ9ERG0.
    PRIDEiQ9ERG0.

    PTM databases

    PhosphoSiteiQ9ERG0.

    Expressioni

    Tissue specificityi

    Isoform Alpha is highly expressed in embryos from day 7-11 and in adult spleen and lung. Isoform Beta expression is highest in adult kidney, testis, lung and liver, intermediate in heart, brain, spleen, skeletal muscle and low in embryos.

    Gene expression databases

    ArrayExpressiQ9ERG0.
    BgeeiQ9ERG0.
    CleanExiMM_LIMA1.
    GenevestigatoriQ9ERG0.

    Interactioni

    Protein-protein interaction databases

    BioGridi211164. 4 interactions.
    DIPiDIP-29634N.
    DIP-29637N.
    IntActiQ9ERG0. 4 interactions.
    MINTiMINT-4094516.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ERG0.
    SMRiQ9ERG0. Positions 372-455.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini386 – 44661LIM zinc-bindingPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    Sequence similaritiesi

    Contains 1 LIM zinc-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    LIM domain

    Phylogenomic databases

    eggNOGiNOG238020.
    GeneTreeiENSGT00510000046839.
    HOGENOMiHOG000059621.
    HOVERGENiHBG051492.
    InParanoidiQ8K2H0.
    OMAiDEISKPE.
    OrthoDBiEOG7PCJH8.
    TreeFamiTF350273.

    Family and domain databases

    Gene3Di2.10.110.10. 1 hit.
    InterProiIPR028740. EPLIN.
    IPR001781. Znf_LIM.
    [Graphical view]
    PANTHERiPTHR24206:SF27. PTHR24206:SF27. 1 hit.
    PfamiPF00412. LIM. 1 hit.
    [Graphical view]
    SMARTiSM00132. LIM. 1 hit.
    [Graphical view]
    PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
    PS50023. LIM_DOMAIN_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative promoter usage. Align

    Isoform Beta (identifier: Q9ERG0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MESTPFNRRQ WTSLSLRVTA KELSLVNKNK SSAIVEIFSK YQKAAEEANM    50
    ERKKNNPESL PQHFRRGTLS VLKKKWENPV AGAEFHTDSL PNSSSEGGHT 100
    ADYPPAEVTD KPAPGVRADR EEHTQPKPRF GSRPEAVIQS RYPRSENSHD 150
    FKAQATESQK MENCLGDSRH EAEKPETSEN TETSGKIEKY NVPLNRLKMM 200
    FEKGEHNQTK SLWTQSRNAG GRRLSENNCS LDDWEIGAGH LSSSAFNSEK 250
    NESKRNLELP RLSETSIKDR MAKYQAAVSK QSSPASYTNE LKTSESKTHK 300
    WEQKENVPPG PEACSVHQEG SKVSTTENSL VALSVPAEDD TCNSQVKSEA 350
    QQPMHPKPLS PDARTSSLPE SSPSKTAKKF QAPAKESCVE CQKTVYPMER 400
    LLANQQVFHI SCFRCSYCNN KLSLGTYASL HGRIYCKPHF NQLFKSKGNY 450
    DEGFGHKQHK DLWASKSDNE ETLGRPAQPP NAGESPHSPG VEDAPIAKVG 500
    VLAASMEAKA SSQREREDKP AETKKLRIAW PPPAELGGSG SALEEGIKVS 550
    KPKWPPEDDV CKTEAPEDVD LDLKKLRRSS SLKERSRPFT VAASFRTSSI 600
    KSPKASSPSL RKGWSESEQS EEFGGGIATM ERKQTENARP SGEKENVGKS 650
    RWQGEEVPRS KDRSSFELES ENFMENGANI AEDDNHVHAQ QSPLEPEAPG 700
    WSGFVDTTAA KEFTTQNQKS QDVGFWEGEV VRELSVEEQI KRNRYYDEDE 750
    DEE 753
    Length:753
    Mass (Da):84,060
    Last modified:July 27, 2011 - v3
    Checksum:i79F98E47C100CF22
    GO
    Isoform Alpha (identifier: Q9ERG0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-160: Missing.

    Show »
    Length:593
    Mass (Da):66,018
    Checksum:i122AB8AA07E1FC0A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti216 – 2161S → N in AAG31148. (PubMed:11179679)Curated
    Sequence conflicti325 – 3251T → A in AAG31147. (PubMed:11179679)Curated
    Sequence conflicti486 – 4861P → S in AAG31147. (PubMed:11179679)Curated
    Sequence conflicti499 – 4991V → A in AAG31147. (PubMed:11179679)Curated
    Sequence conflicti538 – 5381G → S in AAG31147. (PubMed:11179679)Curated
    Sequence conflicti605 – 6051A → T in AAG31147. (PubMed:11179679)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 160160Missing in isoform Alpha. 1 PublicationVSP_003118Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF307844 mRNA. Translation: AAG31147.1.
    AF307845 mRNA. Translation: AAG31148.1.
    BC031490 mRNA. Translation: AAH31490.1.
    CH466550 Genomic DNA. Translation: EDL04110.1.
    CCDSiCCDS37206.1. [Q9ERG0-2]
    CCDS49729.1. [Q9ERG0-1]
    RefSeqiNP_001107017.1. NM_001113545.1. [Q9ERG0-1]
    UniGeneiMm.33207.

    Genome annotation databases

    EnsembliENSMUST00000073691; ENSMUSP00000073371; ENSMUSG00000023022. [Q9ERG0-1]
    ENSMUST00000109024; ENSMUSP00000104652; ENSMUSG00000023022. [Q9ERG0-2]
    GeneIDi65970.
    KEGGimmu:65970.
    UCSCiuc007xqh.2. mouse. [Q9ERG0-1]

    Keywords - Coding sequence diversityi

    Alternative promoter usage

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF307844 mRNA. Translation: AAG31147.1 .
    AF307845 mRNA. Translation: AAG31148.1 .
    BC031490 mRNA. Translation: AAH31490.1 .
    CH466550 Genomic DNA. Translation: EDL04110.1 .
    CCDSi CCDS37206.1. [Q9ERG0-2 ]
    CCDS49729.1. [Q9ERG0-1 ]
    RefSeqi NP_001107017.1. NM_001113545.1. [Q9ERG0-1 ]
    UniGenei Mm.33207.

    3D structure databases

    ProteinModelPortali Q9ERG0.
    SMRi Q9ERG0. Positions 372-455.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 211164. 4 interactions.
    DIPi DIP-29634N.
    DIP-29637N.
    IntActi Q9ERG0. 4 interactions.
    MINTi MINT-4094516.

    PTM databases

    PhosphoSitei Q9ERG0.

    Proteomic databases

    MaxQBi Q9ERG0.
    PaxDbi Q9ERG0.
    PRIDEi Q9ERG0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000073691 ; ENSMUSP00000073371 ; ENSMUSG00000023022 . [Q9ERG0-1 ]
    ENSMUST00000109024 ; ENSMUSP00000104652 ; ENSMUSG00000023022 . [Q9ERG0-2 ]
    GeneIDi 65970.
    KEGGi mmu:65970.
    UCSCi uc007xqh.2. mouse. [Q9ERG0-1 ]

    Organism-specific databases

    CTDi 51474.
    MGIi MGI:1920992. Lima1.

    Phylogenomic databases

    eggNOGi NOG238020.
    GeneTreei ENSGT00510000046839.
    HOGENOMi HOG000059621.
    HOVERGENi HBG051492.
    InParanoidi Q8K2H0.
    OMAi DEISKPE.
    OrthoDBi EOG7PCJH8.
    TreeFami TF350273.

    Miscellaneous databases

    ChiTaRSi LIMA1. mouse.
    NextBioi 320412.
    PROi Q9ERG0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9ERG0.
    Bgeei Q9ERG0.
    CleanExi MM_LIMA1.
    Genevestigatori Q9ERG0.

    Family and domain databases

    Gene3Di 2.10.110.10. 1 hit.
    InterProi IPR028740. EPLIN.
    IPR001781. Znf_LIM.
    [Graphical view ]
    PANTHERi PTHR24206:SF27. PTHR24206:SF27. 1 hit.
    Pfami PF00412. LIM. 1 hit.
    [Graphical view ]
    SMARTi SM00132. LIM. 1 hit.
    [Graphical view ]
    PROSITEi PS00478. LIM_DOMAIN_1. 1 hit.
    PS50023. LIM_DOMAIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of mouse epithelial protein lost in neoplasm (EPLIN) and comparison of mammalian and zebrafish EPLIN."
      Maul R.S., Sachi Gerbin C., Chang D.D.
      Gene 262:155-160(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225; SER-230; SER-360; SER-485 AND SER-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
      J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225 AND SER-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-437, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiLIMA1_MOUSE
    AccessioniPrimary (citable) accession number: Q9ERG0
    Secondary accession number(s): Q8K2H0, Q9ERG1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 5, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 99 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3