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Protein

LIM domain and actin-binding protein 1

Gene

Lima1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
LIM domain and actin-binding protein 1
Alternative name(s):
Epithelial protein lost in neoplasm
Short name:
mEPLIN
Gene namesi
Name:Lima1
Synonyms:D15Ertd366e, Eplin
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1920992. Lima1.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • brush border Source: UniProtKB
  • cell-cell adherens junction Source: MGI
  • cleavage furrow Source: UniProtKB
  • cytoplasm Source: MGI
  • focal adhesion Source: UniProtKB
  • plasma membrane Source: MGI
  • stress fiber Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000757311 – 753LIM domain and actin-binding protein 1Add BLAST753

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei15PhosphoserineBy similarity1
Modified residuei132PhosphoserineCombined sources1
Modified residuei225PhosphoserineCombined sources1
Modified residuei230PhosphoserineCombined sources1
Modified residuei242PhosphoserineCombined sources1
Modified residuei263PhosphoserineBy similarity1
Modified residuei348PhosphoserineBy similarity1
Modified residuei360PhosphoserineCombined sources1
Modified residuei367PhosphoserineBy similarity1
Modified residuei372PhosphoserineBy similarity1
Modified residuei437N6-succinyllysineCombined sources1
Modified residuei467PhosphoserineCombined sources1
Modified residuei485PhosphoserineCombined sources1
Modified residuei488PhosphoserineCombined sources1
Modified residuei599PhosphoserineBy similarity1
Modified residuei602PhosphoserineBy similarity1
Modified residuei607PhosphoserineBy similarity1
Modified residuei615PhosphoserineCombined sources1
Modified residuei692PhosphoserineBy similarity1
Modified residuei720PhosphoserineCombined sources1
Modified residuei735PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9ERG0.
PeptideAtlasiQ9ERG0.
PRIDEiQ9ERG0.

PTM databases

iPTMnetiQ9ERG0.
PhosphoSitePlusiQ9ERG0.
SwissPalmiQ9ERG0.

Expressioni

Tissue specificityi

Isoform Alpha is highly expressed in embryos from day 7-11 and in adult spleen and lung. Isoform Beta expression is highest in adult kidney, testis, lung and liver, intermediate in heart, brain, spleen, skeletal muscle and low in embryos.

Gene expression databases

BgeeiENSMUSG00000023022.
CleanExiMM_LIMA1.
ExpressionAtlasiQ9ERG0. baseline and differential.
GenevisibleiQ9ERG0. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211164. 132 interactors.
DIPiDIP-29634N.
DIP-29637N.
IntActiQ9ERG0. 134 interactors.
MINTiMINT-4094516.
STRINGi10090.ENSMUSP00000073371.

Structurei

3D structure databases

ProteinModelPortaliQ9ERG0.
SMRiQ9ERG0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini386 – 446LIM zinc-bindingPROSITE-ProRule annotationAdd BLAST61

Domaini

Sequence similaritiesi

Contains 1 LIM zinc-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain

Phylogenomic databases

eggNOGiENOG410ITI7. Eukaryota.
ENOG41102B0. LUCA.
GeneTreeiENSGT00510000046839.
HOGENOMiHOG000059621.
HOVERGENiHBG051492.
InParanoidiQ9ERG0.
OMAiKMENCLG.
OrthoDBiEOG091G0043.
TreeFamiTF350273.

Family and domain databases

Gene3Di2.10.110.10. 1 hit.
InterProiIPR028740. EPLIN.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24206:SF34. PTHR24206:SF34. 2 hits.
PfamiPF00412. LIM. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 1 hit.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform Beta (identifier: Q9ERG0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESTPFNRRQ WTSLSLRVTA KELSLVNKNK SSAIVEIFSK YQKAAEEANM
60 70 80 90 100
ERKKNNPESL PQHFRRGTLS VLKKKWENPV AGAEFHTDSL PNSSSEGGHT
110 120 130 140 150
ADYPPAEVTD KPAPGVRADR EEHTQPKPRF GSRPEAVIQS RYPRSENSHD
160 170 180 190 200
FKAQATESQK MENCLGDSRH EAEKPETSEN TETSGKIEKY NVPLNRLKMM
210 220 230 240 250
FEKGEHNQTK SLWTQSRNAG GRRLSENNCS LDDWEIGAGH LSSSAFNSEK
260 270 280 290 300
NESKRNLELP RLSETSIKDR MAKYQAAVSK QSSPASYTNE LKTSESKTHK
310 320 330 340 350
WEQKENVPPG PEACSVHQEG SKVSTTENSL VALSVPAEDD TCNSQVKSEA
360 370 380 390 400
QQPMHPKPLS PDARTSSLPE SSPSKTAKKF QAPAKESCVE CQKTVYPMER
410 420 430 440 450
LLANQQVFHI SCFRCSYCNN KLSLGTYASL HGRIYCKPHF NQLFKSKGNY
460 470 480 490 500
DEGFGHKQHK DLWASKSDNE ETLGRPAQPP NAGESPHSPG VEDAPIAKVG
510 520 530 540 550
VLAASMEAKA SSQREREDKP AETKKLRIAW PPPAELGGSG SALEEGIKVS
560 570 580 590 600
KPKWPPEDDV CKTEAPEDVD LDLKKLRRSS SLKERSRPFT VAASFRTSSI
610 620 630 640 650
KSPKASSPSL RKGWSESEQS EEFGGGIATM ERKQTENARP SGEKENVGKS
660 670 680 690 700
RWQGEEVPRS KDRSSFELES ENFMENGANI AEDDNHVHAQ QSPLEPEAPG
710 720 730 740 750
WSGFVDTTAA KEFTTQNQKS QDVGFWEGEV VRELSVEEQI KRNRYYDEDE

DEE
Length:753
Mass (Da):84,060
Last modified:July 27, 2011 - v3
Checksum:i79F98E47C100CF22
GO
Isoform Alpha (identifier: Q9ERG0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-160: Missing.

Show »
Length:593
Mass (Da):66,018
Checksum:i122AB8AA07E1FC0A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti216S → N in AAG31148 (PubMed:11179679).Curated1
Sequence conflicti325T → A in AAG31147 (PubMed:11179679).Curated1
Sequence conflicti486P → S in AAG31147 (PubMed:11179679).Curated1
Sequence conflicti499V → A in AAG31147 (PubMed:11179679).Curated1
Sequence conflicti538G → S in AAG31147 (PubMed:11179679).Curated1
Sequence conflicti605A → T in AAG31147 (PubMed:11179679).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0031181 – 160Missing in isoform Alpha. 1 PublicationAdd BLAST160

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF307844 mRNA. Translation: AAG31147.1.
AF307845 mRNA. Translation: AAG31148.1.
BC031490 mRNA. Translation: AAH31490.1.
CH466550 Genomic DNA. Translation: EDL04110.1.
CCDSiCCDS37206.1. [Q9ERG0-2]
CCDS49729.1. [Q9ERG0-1]
RefSeqiNP_001107017.1. NM_001113545.1. [Q9ERG0-1]
UniGeneiMm.33207.

Genome annotation databases

EnsembliENSMUST00000073691; ENSMUSP00000073371; ENSMUSG00000023022. [Q9ERG0-1]
ENSMUST00000109024; ENSMUSP00000104652; ENSMUSG00000023022. [Q9ERG0-2]
GeneIDi65970.
KEGGimmu:65970.
UCSCiuc007xqh.3. mouse. [Q9ERG0-1]

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF307844 mRNA. Translation: AAG31147.1.
AF307845 mRNA. Translation: AAG31148.1.
BC031490 mRNA. Translation: AAH31490.1.
CH466550 Genomic DNA. Translation: EDL04110.1.
CCDSiCCDS37206.1. [Q9ERG0-2]
CCDS49729.1. [Q9ERG0-1]
RefSeqiNP_001107017.1. NM_001113545.1. [Q9ERG0-1]
UniGeneiMm.33207.

3D structure databases

ProteinModelPortaliQ9ERG0.
SMRiQ9ERG0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211164. 132 interactors.
DIPiDIP-29634N.
DIP-29637N.
IntActiQ9ERG0. 134 interactors.
MINTiMINT-4094516.
STRINGi10090.ENSMUSP00000073371.

PTM databases

iPTMnetiQ9ERG0.
PhosphoSitePlusiQ9ERG0.
SwissPalmiQ9ERG0.

Proteomic databases

PaxDbiQ9ERG0.
PeptideAtlasiQ9ERG0.
PRIDEiQ9ERG0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073691; ENSMUSP00000073371; ENSMUSG00000023022. [Q9ERG0-1]
ENSMUST00000109024; ENSMUSP00000104652; ENSMUSG00000023022. [Q9ERG0-2]
GeneIDi65970.
KEGGimmu:65970.
UCSCiuc007xqh.3. mouse. [Q9ERG0-1]

Organism-specific databases

CTDi51474.
MGIiMGI:1920992. Lima1.

Phylogenomic databases

eggNOGiENOG410ITI7. Eukaryota.
ENOG41102B0. LUCA.
GeneTreeiENSGT00510000046839.
HOGENOMiHOG000059621.
HOVERGENiHBG051492.
InParanoidiQ9ERG0.
OMAiKMENCLG.
OrthoDBiEOG091G0043.
TreeFamiTF350273.

Miscellaneous databases

ChiTaRSiLima1. mouse.
PROiQ9ERG0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023022.
CleanExiMM_LIMA1.
ExpressionAtlasiQ9ERG0. baseline and differential.
GenevisibleiQ9ERG0. MM.

Family and domain databases

Gene3Di2.10.110.10. 1 hit.
InterProiIPR028740. EPLIN.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24206:SF34. PTHR24206:SF34. 2 hits.
PfamiPF00412. LIM. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 1 hit.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIMA1_MOUSE
AccessioniPrimary (citable) accession number: Q9ERG0
Secondary accession number(s): Q8K2H0, Q9ERG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.