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Q9ERG0

- LIMA1_MOUSE

UniProt

Q9ERG0 - LIMA1_MOUSE

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Protein
LIM domain and actin-binding protein 1
Gene
Lima1, D15Ertd366e, Eplin
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments By similarity.

GO - Molecular functioni

  1. actin filament binding Source: UniProtKB
  2. actin monomer binding Source: UniProtKB
  3. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. actin filament bundle assembly Source: UniProtKB
  2. negative regulation of actin filament depolymerization Source: UniProtKB
  3. ruffle organization Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
LIM domain and actin-binding protein 1
Alternative name(s):
Epithelial protein lost in neoplasm
Short name:
mEPLIN
Gene namesi
Name:Lima1
Synonyms:D15Ertd366e, Eplin
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:1920992. Lima1.

Subcellular locationi

Cytoplasm. Cytoplasmcytoskeleton
Note: This cytoskeletal protein colocalizes with actin stress fibers.

GO - Cellular componenti

  1. actin cytoskeleton Source: MGI
  2. cytoplasm Source: UniProtKB-SubCell
  3. focal adhesion Source: UniProtKB
  4. plasma membrane Source: Ensembl
  5. stress fiber Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 753753LIM domain and actin-binding protein 1
PRO_0000075731Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei15 – 151Phosphoserine By similarity
Modified residuei132 – 1321Phosphoserine By similarity
Modified residuei225 – 2251Phosphoserine2 Publications
Modified residuei230 – 2301Phosphoserine1 Publication
Modified residuei263 – 2631Phosphoserine By similarity
Modified residuei348 – 3481Phosphoserine By similarity
Modified residuei360 – 3601Phosphoserine1 Publication
Modified residuei367 – 3671Phosphoserine By similarity
Modified residuei372 – 3721Phosphoserine By similarity
Modified residuei437 – 4371N6-succinyllysine1 Publication
Modified residuei485 – 4851Phosphoserine1 Publication
Modified residuei488 – 4881Phosphoserine4 Publications
Modified residuei602 – 6021Phosphoserine By similarity
Modified residuei607 – 6071Phosphoserine By similarity
Modified residuei615 – 6151Phosphoserine By similarity
Modified residuei692 – 6921Phosphoserine By similarity
Modified residuei720 – 7201Phosphoserine By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9ERG0.
PaxDbiQ9ERG0.
PRIDEiQ9ERG0.

PTM databases

PhosphoSiteiQ9ERG0.

Expressioni

Tissue specificityi

Isoform Alpha is highly expressed in embryos from day 7-11 and in adult spleen and lung. Isoform Beta expression is highest in adult kidney, testis, lung and liver, intermediate in heart, brain, spleen, skeletal muscle and low in embryos.

Gene expression databases

ArrayExpressiQ9ERG0.
BgeeiQ9ERG0.
CleanExiMM_LIMA1.
GenevestigatoriQ9ERG0.

Interactioni

Protein-protein interaction databases

BioGridi211164. 4 interactions.
DIPiDIP-29634N.
DIP-29637N.
IntActiQ9ERG0. 4 interactions.
MINTiMINT-4094516.

Structurei

3D structure databases

ProteinModelPortaliQ9ERG0.
SMRiQ9ERG0. Positions 372-455.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini386 – 44661LIM zinc-binding
Add
BLAST

Domaini

Sequence similaritiesi

Keywords - Domaini

LIM domain

Phylogenomic databases

eggNOGiNOG238020.
GeneTreeiENSGT00510000046839.
HOGENOMiHOG000059621.
HOVERGENiHBG051492.
InParanoidiQ8K2H0.
OMAiDEISKPE.
OrthoDBiEOG7PCJH8.
TreeFamiTF350273.

Family and domain databases

Gene3Di2.10.110.10. 1 hit.
InterProiIPR028740. EPLIN.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24206:SF27. PTHR24206:SF27. 1 hit.
PfamiPF00412. LIM. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 1 hit.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. Align

Isoform Beta (identifier: Q9ERG0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MESTPFNRRQ WTSLSLRVTA KELSLVNKNK SSAIVEIFSK YQKAAEEANM    50
ERKKNNPESL PQHFRRGTLS VLKKKWENPV AGAEFHTDSL PNSSSEGGHT 100
ADYPPAEVTD KPAPGVRADR EEHTQPKPRF GSRPEAVIQS RYPRSENSHD 150
FKAQATESQK MENCLGDSRH EAEKPETSEN TETSGKIEKY NVPLNRLKMM 200
FEKGEHNQTK SLWTQSRNAG GRRLSENNCS LDDWEIGAGH LSSSAFNSEK 250
NESKRNLELP RLSETSIKDR MAKYQAAVSK QSSPASYTNE LKTSESKTHK 300
WEQKENVPPG PEACSVHQEG SKVSTTENSL VALSVPAEDD TCNSQVKSEA 350
QQPMHPKPLS PDARTSSLPE SSPSKTAKKF QAPAKESCVE CQKTVYPMER 400
LLANQQVFHI SCFRCSYCNN KLSLGTYASL HGRIYCKPHF NQLFKSKGNY 450
DEGFGHKQHK DLWASKSDNE ETLGRPAQPP NAGESPHSPG VEDAPIAKVG 500
VLAASMEAKA SSQREREDKP AETKKLRIAW PPPAELGGSG SALEEGIKVS 550
KPKWPPEDDV CKTEAPEDVD LDLKKLRRSS SLKERSRPFT VAASFRTSSI 600
KSPKASSPSL RKGWSESEQS EEFGGGIATM ERKQTENARP SGEKENVGKS 650
RWQGEEVPRS KDRSSFELES ENFMENGANI AEDDNHVHAQ QSPLEPEAPG 700
WSGFVDTTAA KEFTTQNQKS QDVGFWEGEV VRELSVEEQI KRNRYYDEDE 750
DEE 753
Length:753
Mass (Da):84,060
Last modified:July 27, 2011 - v3
Checksum:i79F98E47C100CF22
GO
Isoform Alpha (identifier: Q9ERG0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-160: Missing.

Show »
Length:593
Mass (Da):66,018
Checksum:i122AB8AA07E1FC0A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 160160Missing in isoform Alpha.
VSP_003118Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 2161S → N in AAG31148. 1 Publication
Sequence conflicti325 – 3251T → A in AAG31147. 1 Publication
Sequence conflicti486 – 4861P → S in AAG31147. 1 Publication
Sequence conflicti499 – 4991V → A in AAG31147. 1 Publication
Sequence conflicti538 – 5381G → S in AAG31147. 1 Publication
Sequence conflicti605 – 6051A → T in AAG31147. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF307844 mRNA. Translation: AAG31147.1.
AF307845 mRNA. Translation: AAG31148.1.
BC031490 mRNA. Translation: AAH31490.1.
CH466550 Genomic DNA. Translation: EDL04110.1.
CCDSiCCDS37206.1. [Q9ERG0-2]
CCDS49729.1. [Q9ERG0-1]
RefSeqiNP_001107017.1. NM_001113545.1. [Q9ERG0-1]
UniGeneiMm.33207.

Genome annotation databases

EnsembliENSMUST00000073691; ENSMUSP00000073371; ENSMUSG00000023022. [Q9ERG0-1]
ENSMUST00000109024; ENSMUSP00000104652; ENSMUSG00000023022. [Q9ERG0-2]
GeneIDi65970.
KEGGimmu:65970.
UCSCiuc007xqh.2. mouse. [Q9ERG0-1]

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF307844 mRNA. Translation: AAG31147.1 .
AF307845 mRNA. Translation: AAG31148.1 .
BC031490 mRNA. Translation: AAH31490.1 .
CH466550 Genomic DNA. Translation: EDL04110.1 .
CCDSi CCDS37206.1. [Q9ERG0-2 ]
CCDS49729.1. [Q9ERG0-1 ]
RefSeqi NP_001107017.1. NM_001113545.1. [Q9ERG0-1 ]
UniGenei Mm.33207.

3D structure databases

ProteinModelPortali Q9ERG0.
SMRi Q9ERG0. Positions 372-455.
ModBasei Search...

Protein-protein interaction databases

BioGridi 211164. 4 interactions.
DIPi DIP-29634N.
DIP-29637N.
IntActi Q9ERG0. 4 interactions.
MINTi MINT-4094516.

PTM databases

PhosphoSitei Q9ERG0.

Proteomic databases

MaxQBi Q9ERG0.
PaxDbi Q9ERG0.
PRIDEi Q9ERG0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000073691 ; ENSMUSP00000073371 ; ENSMUSG00000023022 . [Q9ERG0-1 ]
ENSMUST00000109024 ; ENSMUSP00000104652 ; ENSMUSG00000023022 . [Q9ERG0-2 ]
GeneIDi 65970.
KEGGi mmu:65970.
UCSCi uc007xqh.2. mouse. [Q9ERG0-1 ]

Organism-specific databases

CTDi 51474.
MGIi MGI:1920992. Lima1.

Phylogenomic databases

eggNOGi NOG238020.
GeneTreei ENSGT00510000046839.
HOGENOMi HOG000059621.
HOVERGENi HBG051492.
InParanoidi Q8K2H0.
OMAi DEISKPE.
OrthoDBi EOG7PCJH8.
TreeFami TF350273.

Miscellaneous databases

ChiTaRSi LIMA1. mouse.
NextBioi 320412.
PROi Q9ERG0.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9ERG0.
Bgeei Q9ERG0.
CleanExi MM_LIMA1.
Genevestigatori Q9ERG0.

Family and domain databases

Gene3Di 2.10.110.10. 1 hit.
InterProi IPR028740. EPLIN.
IPR001781. Znf_LIM.
[Graphical view ]
PANTHERi PTHR24206:SF27. PTHR24206:SF27. 1 hit.
Pfami PF00412. LIM. 1 hit.
[Graphical view ]
SMARTi SM00132. LIM. 1 hit.
[Graphical view ]
PROSITEi PS00478. LIM_DOMAIN_1. 1 hit.
PS50023. LIM_DOMAIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of mouse epithelial protein lost in neoplasm (EPLIN) and comparison of mammalian and zebrafish EPLIN."
    Maul R.S., Sachi Gerbin C., Chang D.D.
    Gene 262:155-160(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225; SER-230; SER-360; SER-485 AND SER-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225 AND SER-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-488, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-437, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiLIMA1_MOUSE
AccessioniPrimary (citable) accession number: Q9ERG0
Secondary accession number(s): Q8K2H0, Q9ERG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 98 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi