Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

C-Jun-amino-terminal kinase-interacting protein 2

Gene

Mapk8ip2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 inhibits IL1 beta-induced apoptosis in insulin-secreting cells (By similarity).By similarity

GO - Molecular functioni

  • kinesin binding Source: UniProtKB
  • protein kinase activator activity Source: MGI
  • protein kinase binding Source: UniProtKB

GO - Biological processi

  • activation of protein kinase activity Source: MGI
  • behavioral fear response Source: BHF-UCL
  • dendrite morphogenesis Source: BHF-UCL
  • excitatory postsynaptic potential Source: BHF-UCL
  • JNK cascade Source: MGI
  • MAPK cascade Source: MGI
  • mating behavior Source: BHF-UCL
  • nonassociative learning Source: BHF-UCL
  • positive regulation of stress-activated MAPK cascade Source: Ensembl
  • regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: BHF-UCL
  • regulation of JNK cascade Source: UniProtKB
  • regulation of N-methyl-D-aspartate selective glutamate receptor activity Source: BHF-UCL
  • regulation of receptor activity Source: BHF-UCL
  • regulation of synaptic transmission, glutamatergic Source: BHF-UCL
  • social behavior Source: BHF-UCL
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
C-Jun-amino-terminal kinase-interacting protein 2
Short name:
JIP-2
Short name:
JNK-interacting protein 2
Alternative name(s):
Islet-brain-2
Short name:
IB-2
JNK MAP kinase scaffold protein 2
Mitogen-activated protein kinase 8-interacting protein 2
Gene namesi
Name:Mapk8ip2
Synonyms:Ib2, Jip2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1926555. Mapk8ip2.

Subcellular locationi

  • Cytoplasm

  • Note: Accumulates in cell surface projections.By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • intracellular Source: MGI
  • neuronal cell body Source: MGI
  • neuronal postsynaptic density Source: BHF-UCL
  • protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 830830C-Jun-amino-terminal kinase-interacting protein 2PRO_0000220632Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei257 – 2571PhosphoserineCombined sources
Modified residuei304 – 3041PhosphoserineCombined sources
Modified residuei307 – 3071PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9ERE9.
PaxDbiQ9ERE9.
PRIDEiQ9ERE9.

PTM databases

iPTMnetiQ9ERE9.
PhosphoSiteiQ9ERE9.

Expressioni

Tissue specificityi

Highly expressed in brain. Expressed in all neurons. Also expressed in testis, primarily in the epididymal epidermis.

Inductioni

Upon neuron differentiation.

Gene expression databases

BgeeiQ9ERE9.
ExpressionAtlasiQ9ERE9. baseline and differential.
GenevisibleiQ9ERE9. MM.

Interactioni

Subunit structurei

Forms homo-or heterooligomeric complexes. Binds specific components of the JNK signaling pathway namely JNK1, JNK2, JNK3, MAP2K7, MAP3K10, MAP3K11, MAP3K12 and MAPK13 (By similarity). Also binds the proline-rich domain-containing splice variant of apolipoprotein E receptor 2 (ApoER2). Binds the TPR motif-containing C-terminal of kinesin light chain. Binds the cytoplasmic tails of LRP1 and LRP2 (Megalin). Interacts with DCLK2. Interacts with FGF13; enables the interaction with MAPK13 and may regulate the MAPK8IP2 scaffolding activity. Interacts with TIAM1 and TIAM2.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ApplP145992EBI-74576,EBI-74135From a different organism.
Lrp1Q91ZX72EBI-74576,EBI-300955
Lrp2A2ARV42EBI-74576,EBI-300875

GO - Molecular functioni

  • kinesin binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi208626. 2 interactions.
IntActiQ9ERE9. 7 interactions.
STRINGi10090.ENSMUSP00000023291.

Structurei

3D structure databases

ProteinModelPortaliQ9ERE9.
SMRiQ9ERE9. Positions 612-666, 677-809.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini610 – 67162SH3PROSITE-ProRule annotationAdd
BLAST
Domaini683 – 819137PIDPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni111 – 278168JNK-binding domain (JBD)Add
BLAST
Regioni242 – 504263Necessary for interaction with FGF13By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi30 – 367Asp/Glu-rich (acidic)
Compositional biasi85 – 10420Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi154 – 1574Poly-Asn
Compositional biasi282 – 29312Ser-richAdd
BLAST
Compositional biasi420 – 43718Pro-richAdd
BLAST
Compositional biasi472 – 48716Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the JIP scaffold family.Curated
Contains 1 PID domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3775. Eukaryota.
ENOG410ZFRJ. LUCA.
GeneTreeiENSGT00390000003908.
HOGENOMiHOG000231470.
HOVERGENiHBG018568.
InParanoidiQ9ERE9.
KOiK04435.
OMAiVCSFQDD.
OrthoDBiEOG74J97M.
PhylomeDBiQ9ERE9.
TreeFamiTF325073.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00640. PID. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ERE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADRAEMFSL STFHSLSPPG CRPPQDISLE EFDDEDLSEI TDDCGLGLSY
60 70 80 90 100
DSDHCEKDSL SLGRSEQPHP ICSFQDDFQE FEMIDDNEEE DDEEEEEEEE
110 120 130 140 150
EEEDGDRQGK AGGGPGSQAL AGDSLIPSPS LEESHKLRPT TLHLTTLGAQ
160 170 180 190 200
DSLNNNNGGF TSAPPSSWQE TVLRSPAQEP LKELPAPLLP AEEERHEVQS
210 220 230 240 250
LARPGCDCEG NQPPEPPASS GGASPSSDPG IEADLRSHSS GGHEGRRSSQ
260 270 280 290 300
ELSSPGSDSE DAGGARLGRM ISSISETELE LSSDGGSSSG RSSHLTNSIE
310 320 330 340 350
EASSPASEPE PEPEPLHEPP RRPAFLPVGQ DDTNSEYESG SESEPDLSED
360 370 380 390 400
ADSPWLLSNL VSRMISEGSS PIRCPGQCLS PAPRLPEEAA SQANSVPQDC
410 420 430 440 450
QDPEAGPHVE LVDMDTLCGP PPPAPAAPRL GPAQPGPCLF LSNPTRDTIT
460 470 480 490 500
PLWATPGRTA RPGRSCSAAC SEEEEEDEEE DEEDEEDAED SVVPPGSRTT
510 520 530 540 550
GSTAPLDASL VYDAVKYTLV VDEHTQLELV SLRRCAGLGN DSEEDSSCEA
560 570 580 590 600
SEEEAGATLL GSDQVPEDAS PDSPDLTFSK KFLNVFVNST SRSSSTESFG
610 620 630 640 650
LFSCVVNGEE REQTHRAVFR FIPRHPDELE LDVDDPVLVE AEEDDFWFRG
660 670 680 690 700
FNMRTGERGV FPAFYAHAVP GPAKDLLGSK RSPCWVDRFD VQFLGSVEVP
710 720 730 740 750
CHQGNGILCA AMQKIATARK LTVHLRPPAS CDLEISLRGV KLSLSGGGPE
760 770 780 790 800
FQRCSHFFQM KNISFCGCHP RNSCYFGFIT KHPLLSRFAC HVFVSQESMR
810 820 830
PVARSVGRAF LEYYQEHLAF ACPTEDIYLE
Length:830
Mass (Da):89,900
Last modified:March 1, 2001 - v1
Checksum:i7EC8EAD19A90163C
GO

Sequence cautioni

The sequence AK014339 differs from that shown. Reason: Erroneous termination at position 701. Translated as Cys.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 2161P → Q in AK014339 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF310135 mRNA. Translation: AAG31800.1.
AK014339 mRNA. No translation available.
CCDSiCCDS27752.1.
RefSeqiNP_068740.3. NM_021921.3.
UniGeneiMm.173337.
Mm.482169.

Genome annotation databases

EnsembliENSMUST00000023291; ENSMUSP00000023291; ENSMUSG00000022619.
GeneIDi60597.
KEGGimmu:60597.
UCSCiuc007xgx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF310135 mRNA. Translation: AAG31800.1.
AK014339 mRNA. No translation available.
CCDSiCCDS27752.1.
RefSeqiNP_068740.3. NM_021921.3.
UniGeneiMm.173337.
Mm.482169.

3D structure databases

ProteinModelPortaliQ9ERE9.
SMRiQ9ERE9. Positions 612-666, 677-809.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208626. 2 interactions.
IntActiQ9ERE9. 7 interactions.
STRINGi10090.ENSMUSP00000023291.

PTM databases

iPTMnetiQ9ERE9.
PhosphoSiteiQ9ERE9.

Proteomic databases

MaxQBiQ9ERE9.
PaxDbiQ9ERE9.
PRIDEiQ9ERE9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023291; ENSMUSP00000023291; ENSMUSG00000022619.
GeneIDi60597.
KEGGimmu:60597.
UCSCiuc007xgx.2. mouse.

Organism-specific databases

CTDi23542.
MGIiMGI:1926555. Mapk8ip2.

Phylogenomic databases

eggNOGiKOG3775. Eukaryota.
ENOG410ZFRJ. LUCA.
GeneTreeiENSGT00390000003908.
HOGENOMiHOG000231470.
HOVERGENiHBG018568.
InParanoidiQ9ERE9.
KOiK04435.
OMAiVCSFQDD.
OrthoDBiEOG74J97M.
PhylomeDBiQ9ERE9.
TreeFamiTF325073.

Miscellaneous databases

NextBioi315027.
PROiQ9ERE9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9ERE9.
ExpressionAtlasiQ9ERE9. baseline and differential.
GenevisibleiQ9ERE9. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00640. PID. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS01179. PID. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH APOER2.
    Strain: BALB/cJ.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryonic head.
  3. "Fibroblast growth factor homologous factors are intracellular signaling proteins."
    Schoorlemmer J., Goldfarb M.
    Curr. Biol. 11:793-797(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FGF13 AND MAPK13.
  4. Cited for: INTERACTION WITH DCLK2.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-257; SER-304 AND SER-307, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Testis.
  6. "The PHCCEx domain of Tiam1/2 is a novel protein- and membrane-binding module."
    Terawaki S., Kitano K., Mori T., Zhai Y., Higuchi Y., Itoh N., Watanabe T., Kaibuchi K., Hakoshima T.
    EMBO J. 29:236-250(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TIAM1 AND TIAM2.

Entry informationi

Entry nameiJIP2_MOUSE
AccessioniPrimary (citable) accession number: Q9ERE9
Secondary accession number(s): Q9CXI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: March 1, 2001
Last modified: March 16, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.