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Protein

Serine/threonine-protein kinase Sgk3

Gene

Sgk3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na+ channels: SCNN1A/ENAC and SCN5A, K+ channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca2+ channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na+/dicarboxylate cotransporter: SLC13A2/NADC1, Na+-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na+/H+ exchanger: SLC9A3/NHE3, and the Na+/K+ ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Two specific sites, one in the kinase domain (Thr-320) and the other in the C-terminal regulatory region (Ser-486), need to be phosphorylated for its full activation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191ATPPROSITE-ProRule annotation1
Active sitei286Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi168 – 176ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2672351 Stimuli-sensing channels

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Sgk3 (EC:2.7.11.1)
Alternative name(s):
Cytokine-independent survival kinase
Serum/glucocorticoid-regulated kinase 3
Serum/glucocorticoid-regulated kinase-like
Gene namesi
Name:Sgk3
Synonyms:Cisk, Sgkl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2182368 Sgk3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi90R → A: Diminishes binding to phosphoinositides. 1 Publication1
Mutagenesisi191K → A: No activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000866501 – 496Serine/threonine-protein kinase Sgk3Add BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei126PhosphoserineCombined sources1
Modified residuei129PhosphoserineCombined sources1
Modified residuei320Phosphothreonine; by PDPK1By similarity1
Modified residuei486PhosphoserineBy similarity1

Post-translational modificationi

Activated by phosphorylation on Ser-486 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-320.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9ERE3
MaxQBiQ9ERE3
PaxDbiQ9ERE3
PRIDEiQ9ERE3

PTM databases

iPTMnetiQ9ERE3
PhosphoSitePlusiQ9ERE3

Expressioni

Tissue specificityi

Widely expressed, predominantly in the heart, spleen and 7-day embryo.

Gene expression databases

BgeeiENSMUSG00000025915
CleanExiMM_SGK3
ExpressionAtlasiQ9ERE3 baseline and differential
GenevisibleiQ9ERE3 MM

Interactioni

Subunit structurei

Interacts with GSK3B and FLII. Interacts with PDPK1 in a phosphorylation-dependent manner.By similarity

Protein-protein interaction databases

BioGridi228417, 1 interactor
CORUMiQ9ERE3
ELMiQ9ERE3
STRINGi10090.ENSMUSP00000095437

Structurei

Secondary structure

1496
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi15 – 26Combined sources12
Beta strandi29 – 40Combined sources12
Beta strandi43 – 50Combined sources8
Helixi51 – 64Combined sources14
Helixi66 – 68Combined sources3
Helixi84 – 101Combined sources18
Helixi105 – 108Combined sources4
Helixi111 – 116Combined sources6
Turni117 – 120Combined sources4
Helixi122 – 124Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XTEX-ray1.60A7-160[»]
1XTNX-ray2.20A/B7-126[»]
ProteinModelPortaliQ9ERE3
SMRiQ9ERE3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ERE3

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 124PXPROSITE-ProRule annotationAdd BLAST113
Domaini162 – 419Protein kinasePROSITE-ProRule annotationAdd BLAST258
Domaini420 – 496AGC-kinase C-terminalAdd BLAST77

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi195 – 205Nuclear localization signalBy similarityAdd BLAST11

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0598 Eukaryota
KOG2101 Eukaryota
ENOG410XNPH LUCA
GeneTreeiENSGT00890000139324
HOGENOMiHOG000233033
HOVERGENiHBG108317
InParanoidiQ9ERE3
KOiK13304
OMAiFTEEMVP
OrthoDBiEOG091G06FF
PhylomeDBiQ9ERE3
TreeFamiTF320906

Family and domain databases

CDDicd06870 PX_CISK, 1 hit
cd05604 STKc_SGK3, 1 hit
Gene3Di3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR037900 CISK_PX
IPR011009 Kinase-like_dom_sf
IPR001683 Phox
IPR017892 Pkinase_C
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR036871 PX_dom_sf
IPR008271 Ser/Thr_kinase_AS
IPR037709 SGK3_dom
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00433 Pkinase_C, 1 hit
PF00787 PX, 1 hit
SMARTiView protein in SMART
SM00312 PX, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS50195 PX, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ERE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRDCIMDYK ESCPSVSIPS SDEHREKKKR FTVYKVLVSV GRSEWFVFRR
60 70 80 90 100
YAEFDKLYNS LKKQFPAMAL KIPAKRIFGD NFDPDFIKQR RAGLNEFIQN
110 120 130 140 150
LVRYPELYNH PDVRAFLQMD SPRHQSDPSE DEDERSTSKP HSTSRNINLG
160 170 180 190 200
PTGNPHAKPT DFDFLKVIGK GSFGKVLLAK RKLDGKFYAV KVLQKKIVLN
210 220 230 240 250
RKEQKHIMAE RNVLLKNVKH PFLVGLHYSF QTTEKLYFVL DFVNGGELFF
260 270 280 290 300
HLQRERSFPE PRARFYAAEI ASALGYLHSI KIVYRDLKPE NILLDSMGHV
310 320 330 340 350
VLTDFGLCKE GIAISDTTTT FCGTPEYLAP EVIRKQPYDN TVDWWCLGAV
360 370 380 390 400
LYEMLYGLPP FYCRDVAEMY DNILHKPLNL RPGVSLTAWS ILEELLEKNR
410 420 430 440 450
QNRLGAKEDF LEIQNHPFFE SLSWTDLVQK KIPPPFNPNV AGPDDIRNFD
460 470 480 490
AVFTEETVPY SVCVSSDYSI VNASVLEADD AFVGFSYAPP SEDLFL
Length:496
Mass (Da):57,145
Last modified:March 1, 2001 - v1
Checksum:i4B7D2804A5948BAD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114R → G in BAC27349 (PubMed:16141072).Curated1
Sequence conflicti204Q → P in BAC27349 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF312007 mRNA Translation: AAG34115.1
AK030314 mRNA Translation: BAC26895.1
AK031133 mRNA Translation: BAC27269.1
AK031328 mRNA Translation: BAC27349.1
AK146561 mRNA Translation: BAE27261.1
AK151181 mRNA Translation: BAE30182.1
AK171120 mRNA Translation: BAE42262.1
AK171186 mRNA Translation: BAE42298.1
AK171959 mRNA Translation: BAE42748.1
AK172323 mRNA Translation: BAE42945.1
CCDSiCCDS14816.1
RefSeqiNP_001032848.1, NM_001037759.1
NP_573483.1, NM_133220.2
NP_808215.2, NM_177547.3
UniGeneiMm.336410

Genome annotation databases

EnsembliENSMUST00000097826; ENSMUSP00000095437; ENSMUSG00000025915
ENSMUST00000166384; ENSMUSP00000130078; ENSMUSG00000025915
ENSMUST00000168907; ENSMUSP00000126861; ENSMUSG00000025915
ENSMUST00000171265; ENSMUSP00000127462; ENSMUSG00000025915
GeneIDi170755
KEGGimmu:170755
UCSCiuc007agu.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSGK3_MOUSE
AccessioniPrimary (citable) accession number: Q9ERE3
Secondary accession number(s): Q3UAY2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: March 1, 2001
Last modified: February 28, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  4. SIMILARITY comments
    Index of protein domains and families

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