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Protein

Tuftelin-interacting protein 11

Gene

Tfip11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. Intron turnover seems to proceed through reactions in two lariat-intron associated complexes termed Intron Large (IL) and Intron Small (IS). In cooperation with DHX15 seems to mediate the transition of the U2, U5 and U6 snRNP-containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns. May play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.2 Publications

GO - Molecular functioni

GO - Biological processi

  • biomineral tissue development Source: UniProtKB-KW
  • cell differentiation Source: UniProtKB
  • extracellular matrix organization Source: UniProtKB
  • negative regulation of protein binding Source: MGI
  • negative regulation of protein complex assembly Source: MGI
  • regulation of transcription, DNA-templated Source: InterPro
  • spliceosomal complex disassembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Biomineralization, mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Tuftelin-interacting protein 11
Alternative name(s):
Septin and tuftelin-interacting protein 1
Short name:
STIP-1
Tuftelin-interacting protein 39
Gene namesi
Name:Tfip11
Synonyms:Stip, Tip39
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1930075. Tfip11.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: In the nucleus localizes to unique speckle domains in close proximity to nuclear speckles and not identical to paraspeckles.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi166G → A: No effect on nuclear speckled pattern localization; when associated with A-168 and R-170. 1 Publication1
Mutagenesisi168G → A: No effect on nuclear speckled pattern localization; when associated with A-166 and R-170. 1 Publication1
Mutagenesisi170G → R: No effect on nuclear speckled pattern localization; when associated with A-166 and A-168. 1 Publication1
Mutagenesisi701 – 706VKDKFN → TTTTT: Predominant cytoplasmic localization. 1 Publication6

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000725021 – 838Tuftelin-interacting protein 11Add BLAST838

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2PhosphoserineBy similarity1
Modified residuei60PhosphoserineCombined sources1
Modified residuei96PhosphoserineCombined sources1
Modified residuei99PhosphoserineBy similarity1
Modified residuei145PhosphoserineBy similarity1
Modified residuei211PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9ERA6.
MaxQBiQ9ERA6.
PaxDbiQ9ERA6.
PRIDEiQ9ERA6.

PTM databases

iPTMnetiQ9ERA6.
PhosphoSitePlusiQ9ERA6.

Expressioni

Tissue specificityi

Widely expressed. In tooth it is expressed in ameloblasts and odontoblasts.

Developmental stagei

Expressed as early as E14 and continues into postnatal development. Within the developing tooth, expression is localized at the apical region of the ameloblast cells and to the apical regions of the newly formed enamel matrix.

Gene expression databases

BgeeiENSMUSG00000029345.
CleanExiMM_TFIP11.
ExpressionAtlasiQ9ERA6. baseline and differential.
GenevisibleiQ9ERA6. MM.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Found in the Intron Large (IL) complex, a post-mRNA release spliceosomal complex containing the excised intron, U2, U5 and U6 snRNPs, and splicing factors. Interacts with TUFT1. Interacts with DHX15; indicative for a recruitment of DHX15 to the IL complex. Interacts with GCFC2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Dhx15O352863EBI-8338752,EBI-8322087

Protein-protein interaction databases

BioGridi207728. 2 interactors.
IntActiQ9ERA6. 3 interactors.
MINTiMINT-4137591.
STRINGi10090.ENSMUSP00000031288.

Structurei

3D structure databases

ProteinModelPortaliQ9ERA6.
SMRiQ9ERA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini150 – 196G-patchPROSITE-ProRule annotationAdd BLAST47

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 51Required for interaction with DHX15By similarityAdd BLAST51
Regioni711 – 735Required for nuclear speckle localizationAdd BLAST25

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi701 – 706Nuclear localization signal6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi15 – 20Poly-Asp6

Sequence similaritiesi

Belongs to the TFP11/STIP family.Curated
Contains 1 G-patch domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2184. Eukaryota.
ENOG410XP4Y. LUCA.
GeneTreeiENSGT00390000012739.
HOGENOMiHOG000230470.
HOVERGENiHBG055868.
InParanoidiQ9ERA6.
KOiK13103.
OMAiDECARVF.
OrthoDBiEOG091G04T2.
PhylomeDBiQ9ERA6.
TreeFamiTF314887.

Family and domain databases

InterProiIPR000467. G_patch_dom.
IPR022783. GCFC_dom.
IPR024933. STIP.
IPR022159. STIP/TFIP11_N.
[Graphical view]
PfamiPF01585. G-patch. 1 hit.
PF07842. GCFC. 1 hit.
PF12457. TIP_N. 1 hit.
[Graphical view]
PIRSFiPIRSF017706. TFIP11. 1 hit.
SMARTiSM00443. G_patch. 1 hit.
[Graphical view]
PROSITEiPS50174. G_PATCH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ERA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSHLYRDG EGHLDDDDDD ERENFEITDW DLQNEFNPNR QRHWQTKEEA
60 70 80 90 100
TYGVWAERDS DEERPSFGGK RARDYSAPVN FISAGLKKGA AEEADSEDSD
110 120 130 140 150
AEEKPVKQED FPKDLGPKKL KTGGNFKPSQ KGFSGGTKSF MDFGSWERHT
160 170 180 190 200
KGIGQKLLQK MGYVPGRGLG KNAQGIINPI EAKQRKGKGA VGAYGSERTT
210 220 230 240 250
QSLQDFPVAD SEEEAEEEFQ KELSQWRKDP SGSKKKPKYS YKTVEELKAK
260 270 280 290 300
GRVSKKLTAP QKELSQVKVI DMTGREQKVY YSYSQISHKH SVPDEGVPLL
310 320 330 340 350
AQLPPTAGKE ARMPGFALPE LEHNLQLLIE RTEQEIIQSD RQLQYERDMV
360 370 380 390 400
VSLSHELEKT AEVLAHEERV ISNLSKVLAL VEECERRMQP HGADPLTLDE
410 420 430 440 450
CARIFETLQD KYYEEYRLAD RADLAVAIVY PLVKDYFKDW HPLEDGSYGT
460 470 480 490 500
QIISKWKSLL ENDQLLSHSS QDLSSDAFHR LMWEVWMPFV RNVVAQWQPR
510 520 530 540 550
NCEPMVDFLD SWAHIIPVWI LDNILDQLIF PKLQKEVDNW NPLTDTVPIH
560 570 580 590 600
SWIHPWLPLM QARLEPLYSP VRSKLSSALQ KWHPSDASAK LILQPWKEVL
610 620 630 640 650
TPGSWEAFML RNIVPKLGMC LGELVINPHQ QHMDAFYWVM DWEGMISVSS
660 670 680 690 700
LVGLLEKHFF PKWLQVLCSW LSNSPNYEEI TKWYLGWKSM FSDQVLAHPS
710 720 730 740 750
VKDKFNEALD IMNRAVSSNV GAYMQPGARE NIAYLTHTER RKDFQYEAMQ
760 770 780 790 800
ERREAENMAQ RGIGVAASSV PMNFKDLIET KAEEHNIVFM PVIGKRHEGK
810 820 830
QLYTFGRIVI YIDRGVVFVQ GEKTWVPTSL QSLIDMAK
Length:838
Mass (Da):96,305
Last modified:March 1, 2001 - v1
Checksum:iB50842BF12733930
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti393A → T in AAH17682 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290474 mRNA. Translation: AAG10198.2.
BC017682 mRNA. Translation: AAH17682.1.
CCDSiCCDS19539.1.
RefSeqiNP_061253.2. NM_018783.4.
UniGeneiMm.172947.

Genome annotation databases

EnsembliENSMUST00000031288; ENSMUSP00000031288; ENSMUSG00000029345.
GeneIDi54723.
KEGGimmu:54723.
UCSCiuc008ysy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF290474 mRNA. Translation: AAG10198.2.
BC017682 mRNA. Translation: AAH17682.1.
CCDSiCCDS19539.1.
RefSeqiNP_061253.2. NM_018783.4.
UniGeneiMm.172947.

3D structure databases

ProteinModelPortaliQ9ERA6.
SMRiQ9ERA6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207728. 2 interactors.
IntActiQ9ERA6. 3 interactors.
MINTiMINT-4137591.
STRINGi10090.ENSMUSP00000031288.

PTM databases

iPTMnetiQ9ERA6.
PhosphoSitePlusiQ9ERA6.

Proteomic databases

EPDiQ9ERA6.
MaxQBiQ9ERA6.
PaxDbiQ9ERA6.
PRIDEiQ9ERA6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031288; ENSMUSP00000031288; ENSMUSG00000029345.
GeneIDi54723.
KEGGimmu:54723.
UCSCiuc008ysy.1. mouse.

Organism-specific databases

CTDi24144.
MGIiMGI:1930075. Tfip11.

Phylogenomic databases

eggNOGiKOG2184. Eukaryota.
ENOG410XP4Y. LUCA.
GeneTreeiENSGT00390000012739.
HOGENOMiHOG000230470.
HOVERGENiHBG055868.
InParanoidiQ9ERA6.
KOiK13103.
OMAiDECARVF.
OrthoDBiEOG091G04T2.
PhylomeDBiQ9ERA6.
TreeFamiTF314887.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiQ9ERA6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029345.
CleanExiMM_TFIP11.
ExpressionAtlasiQ9ERA6. baseline and differential.
GenevisibleiQ9ERA6. MM.

Family and domain databases

InterProiIPR000467. G_patch_dom.
IPR022783. GCFC_dom.
IPR024933. STIP.
IPR022159. STIP/TFIP11_N.
[Graphical view]
PfamiPF01585. G-patch. 1 hit.
PF07842. GCFC. 1 hit.
PF12457. TIP_N. 1 hit.
[Graphical view]
PIRSFiPIRSF017706. TFIP11. 1 hit.
SMARTiSM00443. G_patch. 1 hit.
[Graphical view]
PROSITEiPS50174. G_PATCH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTFP11_MOUSE
AccessioniPrimary (citable) accession number: Q9ERA6
Secondary accession number(s): Q8VD06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.