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Q9ERA0 (TFCP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-globin transcription factor CP2
Gene names
Name:Tfcp2
Synonyms:Tcfcp2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds a variety of cellular promoters including fibrinogen, alpha-globin promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1 By similarity. Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter By similarity.

Subunit structure

Binds to DNA as a dimer. Interacts with UBP1 and PIAS1, and is probably part of a complex containing TFCP2, UBP1 and PIAS1. Component of the SSP (stage selector protein) complex, which appears to be a heteromer of TFCP2 and 2 copies of NFE4 By similarity. Ref.4

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the grh/CP2 family. CP2 subfamily.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandDNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpositive regulation of transcription, DNA-templated

Traceable author statement PubMed 11073954. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

transcription factor complex

Traceable author statement PubMed 11073954. Source: MGI

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9ERA0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9ERA0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     474-502: MVQNFQEEACFILDTMEAETSDSYHVILK → VVLSCLILPGTELWSSGRTAGTLNCLALPFL
Note: No experimental confirmation available.
Isoform 3 (identifier: Q9ERA0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     354-355: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502Alpha-globin transcription factor CP2
PRO_0000228002

Regions

Region133 – 386254DNA-binding By similarity

Natural variations

Alternative sequence354 – 3552Missing in isoform 3.
VSP_017649
Alternative sequence474 – 50229MVQNF…HVILK → VVLSCLILPGTELWSSGRTA GTLNCLALPFL in isoform 2.
VSP_017650

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 02D43CAA95CDF495

FASTA50257,031
        10         20         30         40         50         60 
MAWALKLPLA DEVIESGLVQ DFDASLSGIG QELGAGAYSM SDVLALPIFK QEESSLPPDN 

        70         80         90        100        110        120 
ENEILPFQYV LCAATSPAVK LHDETLTYLN QGQSYEIRML DNRKLGELPE LNGKLVKSIF 

       130        140        150        160        170        180 
RVVFHDRRLQ YTEHQQLEGW RWNRPGDRIL DIDIPMSVGV IDPRANPTQL NTVEFLWDPS 

       190        200        210        220        230        240 
KRTSVFIQVH CISTEFTMRK HGGEKGVPFR VQIDTFKENG NGEYTEHLHS ASCQIKVFKP 

       250        260        270        280        290        300 
KGADRKQKID REKMEKRTPH EKEKYQPSYE TTILTECSPW PEITYVNNSP SPGFNSSHSS 

       310        320        330        340        350        360 
FSLGEGNGSP NHQPEPPPPV TDNLLPTTTP QEAQQWLHRN RFSTFTRLFT NFSGADLLKL 

       370        380        390        400        410        420 
TRDDVIQICG PADGIRLFNA LKGRMVRPRL TIYVCQESLQ LREQQPQPQP QPQKQEDGDS 

       430        440        450        460        470        480 
NGTFFVYHAI YLEELTAVEL TEKIAQLFSI SPHQISQIYK QGPTGIHVVI SDEMVQNFQE 

       490        500 
EACFILDTME AETSDSYHVI LK 

« Hide

Isoform 2 [UniParc].

Checksum: D53015C81567A103
Show »

FASTA50456,884
Isoform 3 [UniParc].

Checksum: D323CB8CE4872858
Show »

FASTA50056,903

References

« Hide 'large scale' references
[1]"Molecular cloning of the alpha-globin transcription factor CP2."
Lim L.C., Swendeman S.L., Sheffery M.
Mol. Cell. Biol. 12:828-835(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Heart, Melanocyte and Visual cortex.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: FVB/N.
Tissue: Liver.
[4]"Erythroid cell-specific alpha-globin gene regulation by the CP2 transcription factor family."
Kang H.C., Chae J.H., Lee Y.H., Park M.-A., Shin J.H., Kim S.-H., Ye S.-K., Cho Y.S., Fiering S., Kim C.G.
Mol. Cell. Biol. 25:6005-6020(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UBP1 AND PIAS1.
Strain: DBA/2.
Tissue: Erythroleukemia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M84809 mRNA. Translation: AAF99390.1.
AK146503 mRNA. Translation: BAE27218.1.
AK147898 mRNA. Translation: BAE28214.1.
AK158682 mRNA. Translation: BAE34609.1.
BC022131 mRNA. Translation: AAH22131.1.
PIRB42030.
RefSeqNP_258437.1. NM_033476.3.
UniGeneMm.219040.

3D structure databases

ProteinModelPortalQ9ERA0.
SMRQ9ERA0. Positions 321-381.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204014. 3 interactions.
IntActQ9ERA0. 2 interactions.

PTM databases

PhosphoSiteQ9ERA0.

Proteomic databases

PaxDbQ9ERA0.
PRIDEQ9ERA0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000009877; ENSMUSP00000009877; ENSMUSG00000009733. [Q9ERA0-1]
GeneID21422.
KEGGmmu:21422.
UCSCuc007xrl.1. mouse. [Q9ERA0-1]
uc007xrm.1. mouse. [Q9ERA0-2]
uc011zzo.1. mouse. [Q9ERA0-3]

Organism-specific databases

CTD7024.
MGIMGI:98509. Tfcp2.

Phylogenomic databases

eggNOGNOG275458.
GeneTreeENSGT00740000115287.
HOGENOMHOG000230625.
HOVERGENHBG053805.
KOK09275.
OMADEVCFVL.
OrthoDBEOG7FBRHB.
PhylomeDBQ9ERA0.
TreeFamTF314132.

Gene expression databases

BgeeQ9ERA0.
CleanExMM_TCFCP2.
GenevestigatorQ9ERA0.

Family and domain databases

InterProIPR007604. CP2.
IPR013761. SAM/pointed.
[Graphical view]
PfamPF04516. CP2. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
ProtoNetSearch...

Other

NextBio300738.
PROQ9ERA0.
SOURCESearch...

Entry information

Entry nameTFCP2_MOUSE
AccessionPrimary (citable) accession number: Q9ERA0
Secondary accession number(s): Q3UGJ4, Q8VC13
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot