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Protein

Alpha-globin transcription factor CP2

Gene

Tfcp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds a variety of cellular promoters including fibrinogen, alpha-globin promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1 (By similarity). Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-globin transcription factor CP2
Gene namesi
Name:Tfcp2
Synonyms:Tcfcp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:98509. Tfcp2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: GO_Central
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Alpha-globin transcription factor CP2PRO_0000228002Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei353 – 3531PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9ERA0.
PaxDbiQ9ERA0.
PRIDEiQ9ERA0.

PTM databases

PhosphoSiteiQ9ERA0.

Expressioni

Gene expression databases

BgeeiQ9ERA0.
CleanExiMM_TCFCP2.
GenevisibleiQ9ERA0. MM.

Interactioni

Subunit structurei

Binds to DNA as a dimer. Interacts with UBP1 and PIAS1, and is probably part of a complex containing TFCP2, UBP1 and PIAS1. Component of the SSP (stage selector protein) complex, which appears to be a heteromer of TFCP2 and 2 copies of NFE4 (By similarity).By similarity

Protein-protein interaction databases

BioGridi204014. 3 interactions.
IntActiQ9ERA0. 2 interactions.
STRINGi10090.ENSMUSP00000009877.

Structurei

3D structure databases

ProteinModelPortaliQ9ERA0.
SMRiQ9ERA0. Positions 321-381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 386254DNA-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the grh/CP2 family. CP2 subfamily.Curated

Phylogenomic databases

eggNOGiNOG275458.
GeneTreeiENSGT00760000119235.
HOGENOMiHOG000230625.
HOVERGENiHBG053805.
InParanoidiQ9ERA0.
KOiK09275.
OMAiDEVCFVL.
OrthoDBiEOG7FBRHB.
PhylomeDBiQ9ERA0.
TreeFamiTF314132.

Family and domain databases

InterProiIPR007604. CP2.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF04516. CP2. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ERA0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAWALKLPLA DEVIESGLVQ DFDASLSGIG QELGAGAYSM SDVLALPIFK
60 70 80 90 100
QEESSLPPDN ENEILPFQYV LCAATSPAVK LHDETLTYLN QGQSYEIRML
110 120 130 140 150
DNRKLGELPE LNGKLVKSIF RVVFHDRRLQ YTEHQQLEGW RWNRPGDRIL
160 170 180 190 200
DIDIPMSVGV IDPRANPTQL NTVEFLWDPS KRTSVFIQVH CISTEFTMRK
210 220 230 240 250
HGGEKGVPFR VQIDTFKENG NGEYTEHLHS ASCQIKVFKP KGADRKQKID
260 270 280 290 300
REKMEKRTPH EKEKYQPSYE TTILTECSPW PEITYVNNSP SPGFNSSHSS
310 320 330 340 350
FSLGEGNGSP NHQPEPPPPV TDNLLPTTTP QEAQQWLHRN RFSTFTRLFT
360 370 380 390 400
NFSGADLLKL TRDDVIQICG PADGIRLFNA LKGRMVRPRL TIYVCQESLQ
410 420 430 440 450
LREQQPQPQP QPQKQEDGDS NGTFFVYHAI YLEELTAVEL TEKIAQLFSI
460 470 480 490 500
SPHQISQIYK QGPTGIHVVI SDEMVQNFQE EACFILDTME AETSDSYHVI

LK
Length:502
Mass (Da):57,031
Last modified:March 1, 2001 - v1
Checksum:i02D43CAA95CDF495
GO
Isoform 2 (identifier: Q9ERA0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     474-502: MVQNFQEEACFILDTMEAETSDSYHVILK → VVLSCLILPGTELWSSGRTAGTLNCLALPFL

Note: No experimental confirmation available.
Show »
Length:504
Mass (Da):56,884
Checksum:iD53015C81567A103
GO
Isoform 3 (identifier: Q9ERA0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     354-355: Missing.

Show »
Length:500
Mass (Da):56,903
Checksum:iD323CB8CE4872858
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei354 – 3552Missing in isoform 3. 1 PublicationVSP_017649
Alternative sequencei474 – 50229MVQNF…HVILK → VVLSCLILPGTELWSSGRTA GTLNCLALPFL in isoform 2. 1 PublicationVSP_017650Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84809 mRNA. Translation: AAF99390.1.
AK146503 mRNA. Translation: BAE27218.1.
AK147898 mRNA. Translation: BAE28214.1.
AK158682 mRNA. Translation: BAE34609.1.
BC022131 mRNA. Translation: AAH22131.1.
CCDSiCCDS27840.1. [Q9ERA0-1]
PIRiB42030.
RefSeqiNP_001276532.1. NM_001289603.1. [Q9ERA0-3]
NP_258437.1. NM_033476.3. [Q9ERA0-1]
UniGeneiMm.219040.

Genome annotation databases

EnsembliENSMUST00000009877; ENSMUSP00000009877; ENSMUSG00000009733. [Q9ERA0-1]
GeneIDi21422.
KEGGimmu:21422.
UCSCiuc007xrl.1. mouse. [Q9ERA0-1]
uc007xrm.1. mouse. [Q9ERA0-2]
uc011zzo.1. mouse. [Q9ERA0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84809 mRNA. Translation: AAF99390.1.
AK146503 mRNA. Translation: BAE27218.1.
AK147898 mRNA. Translation: BAE28214.1.
AK158682 mRNA. Translation: BAE34609.1.
BC022131 mRNA. Translation: AAH22131.1.
CCDSiCCDS27840.1. [Q9ERA0-1]
PIRiB42030.
RefSeqiNP_001276532.1. NM_001289603.1. [Q9ERA0-3]
NP_258437.1. NM_033476.3. [Q9ERA0-1]
UniGeneiMm.219040.

3D structure databases

ProteinModelPortaliQ9ERA0.
SMRiQ9ERA0. Positions 321-381.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204014. 3 interactions.
IntActiQ9ERA0. 2 interactions.
STRINGi10090.ENSMUSP00000009877.

PTM databases

PhosphoSiteiQ9ERA0.

Proteomic databases

MaxQBiQ9ERA0.
PaxDbiQ9ERA0.
PRIDEiQ9ERA0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000009877; ENSMUSP00000009877; ENSMUSG00000009733. [Q9ERA0-1]
GeneIDi21422.
KEGGimmu:21422.
UCSCiuc007xrl.1. mouse. [Q9ERA0-1]
uc007xrm.1. mouse. [Q9ERA0-2]
uc011zzo.1. mouse. [Q9ERA0-3]

Organism-specific databases

CTDi7024.
MGIiMGI:98509. Tfcp2.

Phylogenomic databases

eggNOGiNOG275458.
GeneTreeiENSGT00760000119235.
HOGENOMiHOG000230625.
HOVERGENiHBG053805.
InParanoidiQ9ERA0.
KOiK09275.
OMAiDEVCFVL.
OrthoDBiEOG7FBRHB.
PhylomeDBiQ9ERA0.
TreeFamiTF314132.

Miscellaneous databases

NextBioi300738.
PROiQ9ERA0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9ERA0.
CleanExiMM_TCFCP2.
GenevisibleiQ9ERA0. MM.

Family and domain databases

InterProiIPR007604. CP2.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF04516. CP2. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the alpha-globin transcription factor CP2."
    Lim L.C., Swendeman S.L., Sheffery M.
    Mol. Cell. Biol. 12:828-835(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Heart, Melanocyte and Visual cortex.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: FVB/N.
    Tissue: Liver.
  4. "Erythroid cell-specific alpha-globin gene regulation by the CP2 transcription factor family."
    Kang H.C., Chae J.H., Lee Y.H., Park M.-A., Shin J.H., Kim S.-H., Ye S.-K., Cho Y.S., Fiering S., Kim C.G.
    Mol. Cell. Biol. 25:6005-6020(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBP1 AND PIAS1.
    Strain: DBA/2.
    Tissue: Erythroleukemia.

Entry informationi

Entry nameiTFCP2_MOUSE
AccessioniPrimary (citable) accession number: Q9ERA0
Secondary accession number(s): Q3UGJ4, Q8VC13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 1, 2001
Last modified: June 24, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.