Q9ERA0 (TFCP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-globin transcription factor CP2

Gene names

Name:	Tfcp2
Synonyms:	Tcfcp2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds a variety of cellular promoters including fibrinogen, alpha-globin promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1 By similarity. Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter By similarity.
Subunit structure	Binds to DNA as a dimer. Interacts with UBP1 and PIAS1, and is probably part of a complex containing TFCP2, UBP1 and PIAS1. Component of the SSP (stage selector protein) complex, which appears to be a heteromer of TFCP2 and 2 copies of NFE4 By similarity. Ref.4
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the grh/CP2 family. CP2 subfamily.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription, DNA-dependent Traceable author statement PubMed 11073954. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	transcription factor complex Traceable author statement PubMed 11073954. Source: MGI
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9ERA0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9ERA0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     474-502: MVQNFQEEACFILDTMEAETSDSYHVILK → VVLSCLILPGTELWSSGRTAGTLNCLALPFL

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9ERA0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     354-355: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Alpha-globin transcription factor CP2		PRO_0000228002
Regions
	Region	133 – 386	254	DNA-binding By similarity
Natural variations
	Alternative sequence	354 – 355	2	Missing in isoform 3.		VSP_017649
	Alternative sequence	474 – 502	29	MVQNF…HVILK → VVLSCLILPGTELWSSGRTA GTLNCLALPFL in isoform 2.		VSP_017650

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 02D43CAA95CDF495	FASTA	502	57,031
	10 20 30 40 50 60 MAWALKLPLA DEVIESGLVQ DFDASLSGIG QELGAGAYSM SDVLALPIFK QEESSLPPDN 70 80 90 100 110 120 ENEILPFQYV LCAATSPAVK LHDETLTYLN QGQSYEIRML DNRKLGELPE LNGKLVKSIF 130 140 150 160 170 180 RVVFHDRRLQ YTEHQQLEGW RWNRPGDRIL DIDIPMSVGV IDPRANPTQL NTVEFLWDPS 190 200 210 220 230 240 KRTSVFIQVH CISTEFTMRK HGGEKGVPFR VQIDTFKENG NGEYTEHLHS ASCQIKVFKP 250 260 270 280 290 300 KGADRKQKID REKMEKRTPH EKEKYQPSYE TTILTECSPW PEITYVNNSP SPGFNSSHSS 310 320 330 340 350 360 FSLGEGNGSP NHQPEPPPPV TDNLLPTTTP QEAQQWLHRN RFSTFTRLFT NFSGADLLKL 370 380 390 400 410 420 TRDDVIQICG PADGIRLFNA LKGRMVRPRL TIYVCQESLQ LREQQPQPQP QPQKQEDGDS 430 440 450 460 470 480 NGTFFVYHAI YLEELTAVEL TEKIAQLFSI SPHQISQIYK QGPTGIHVVI SDEMVQNFQE 490 500 EACFILDTME AETSDSYHVI LK
	Isoform 2 [UniParc]. Checksum: D53015C81567A103	FASTA	504	56,884
	10 20 30 40 50 60 MAWALKLPLA DEVIESGLVQ DFDASLSGIG QELGAGAYSM SDVLALPIFK QEESSLPPDN 70 80 90 100 110 120 ENEILPFQYV LCAATSPAVK LHDETLTYLN QGQSYEIRML DNRKLGELPE LNGKLVKSIF 130 140 150 160 170 180 RVVFHDRRLQ YTEHQQLEGW RWNRPGDRIL DIDIPMSVGV IDPRANPTQL NTVEFLWDPS 190 200 210 220 230 240 KRTSVFIQVH CISTEFTMRK HGGEKGVPFR VQIDTFKENG NGEYTEHLHS ASCQIKVFKP 250 260 270 280 290 300 KGADRKQKID REKMEKRTPH EKEKYQPSYE TTILTECSPW PEITYVNNSP SPGFNSSHSS 310 320 330 340 350 360 FSLGEGNGSP NHQPEPPPPV TDNLLPTTTP QEAQQWLHRN RFSTFTRLFT NFSGADLLKL 370 380 390 400 410 420 TRDDVIQICG PADGIRLFNA LKGRMVRPRL TIYVCQESLQ LREQQPQPQP QPQKQEDGDS 430 440 450 460 470 480 NGTFFVYHAI YLEELTAVEL TEKIAQLFSI SPHQISQIYK QGPTGIHVVI SDEVVLSCLI 490 500 LPGTELWSSG RTAGTLNCLA LPFL
	Isoform 3 [UniParc]. Checksum: D323CB8CE4872858	FASTA	500	56,903
	10 20 30 40 50 60 MAWALKLPLA DEVIESGLVQ DFDASLSGIG QELGAGAYSM SDVLALPIFK QEESSLPPDN 70 80 90 100 110 120 ENEILPFQYV LCAATSPAVK LHDETLTYLN QGQSYEIRML DNRKLGELPE LNGKLVKSIF 130 140 150 160 170 180 RVVFHDRRLQ YTEHQQLEGW RWNRPGDRIL DIDIPMSVGV IDPRANPTQL NTVEFLWDPS 190 200 210 220 230 240 KRTSVFIQVH CISTEFTMRK HGGEKGVPFR VQIDTFKENG NGEYTEHLHS ASCQIKVFKP 250 260 270 280 290 300 KGADRKQKID REKMEKRTPH EKEKYQPSYE TTILTECSPW PEITYVNNSP SPGFNSSHSS 310 320 330 340 350 360 FSLGEGNGSP NHQPEPPPPV TDNLLPTTTP QEAQQWLHRN RFSTFTRLFT NFSDLLKLTR 370 380 390 400 410 420 DDVIQICGPA DGIRLFNALK GRMVRPRLTI YVCQESLQLR EQQPQPQPQP QKQEDGDSNG 430 440 450 460 470 480 TFFVYHAIYL EELTAVELTE KIAQLFSISP HQISQIYKQG PTGIHVVISD EMVQNFQEEA 490 500 CFILDTMEAE TSDSYHVILK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the alpha-globin transcription factor CP2." Lim L.C., Swendeman S.L., Sheffery M. Mol. Cell. Biol. 12:828-835(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Heart, Melanocyte and Visual cortex.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: FVB/N. Tissue: Liver.
[4]	"Erythroid cell-specific alpha-globin gene regulation by the CP2 transcription factor family." Kang H.C., Chae J.H., Lee Y.H., Park M.-A., Shin J.H., Kim S.-H., Ye S.-K., Cho Y.S., Fiering S., Kim C.G. Mol. Cell. Biol. 25:6005-6020(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UBP1 AND PIAS1. Strain: DBA/2. Tissue: Erythroleukemia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84809 mRNA. Translation: AAF99390.1. AK146503 mRNA. Translation: BAE27218.1. AK147898 mRNA. Translation: BAE28214.1. AK158682 mRNA. Translation: BAE34609.1. BC022131 mRNA. Translation: AAH22131.1.
IPI	IPI00112098. IPI00742365. IPI00742434.
PIR	B42030.
RefSeq	NP_258437.1. NM_033476.2.
UniGene	Mm.219040.
3D structure databases
ProteinModelPortal	Q9ERA0.
SMR	Q9ERA0. Positions 321-381.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ERA0. 2 interactions.
PTM databases
PhosphoSite	Q9ERA0.
Proteomic databases
PaxDb	Q9ERA0.
PRIDE	Q9ERA0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000009877; ENSMUSP00000009877; ENSMUSG00000009733.
GeneID	21422.
KEGG	mmu:21422.
UCSC	uc007xrl.1. mouse. uc007xrm.1. mouse. uc011zzo.1. mouse.
Organism-specific databases
CTD	7024.
MGI	MGI:98509. Tfcp2.
Phylogenomic databases
eggNOG	NOG275458.
GeneTree	ENSGT00550000074370.
HOGENOM	HOG000230625.
HOVERGEN	HBG053805.
KO	K09275.
OMA	FKQEESN.
OrthoDB	EOG4K3KW2.
Gene expression databases
Bgee	Q9ERA0.
CleanEx	MM_TCFCP2.
Genevestigator	Q9ERA0.
GermOnline	ENSMUSG00000009733. Mus musculus.
Family and domain databases
InterPro	IPR007604. CP2. IPR013761. SAM/pointed. [Graphical view]
Pfam	PF04516. CP2. 1 hit. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 1 hit.
ProtoNet	Search...
Other
NextBio	300738.
SOURCE	Search...

Entry information

Entry name

TFCP2_MOUSE

Accession

Primary (citable) accession number: Q9ERA0
Secondary accession number(s): Q3UGJ4, Q8VC13

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program