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Protein

Neuroglobin

Gene

Ngb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64Iron (heme distal ligand)Combined sources1 Publication1
Metal bindingi96Iron (heme proximal ligand)Combined sources2 Publications1

GO - Molecular functioni

  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • oxygen binding Source: InterPro
  • oxygen carrier activity Source: MGI

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • oxygen transport Source: MGI

Keywordsi

Biological processApoptosis, Oxygen transport, Transport
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-8981607 Intracellular oxygen transport

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroglobin
Gene namesi
Name:Ngb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2151886 Ngb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi64H → L: Improved binding of dioxygen and carbon monoxide to the iron atom. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000533921 – 151NeuroglobinAdd BLAST151

Post-translational modificationi

A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO.By similarity
Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9ER97
PRIDEiQ9ER97

Expressioni

Tissue specificityi

Predominantly expressed in brain.

Gene expression databases

BgeeiENSMUSG00000021032
CleanExiMM_NGB
ExpressionAtlasiQ9ER97 baseline and differential
GenevisibleiQ9ER97 MM

Interactioni

Subunit structurei

Monomer. Homodimer and homotetramer; disulfide-linked (Probable). Interacts with 14-3-3 (By similarity).By similarityCurated

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021420

Structurei

Secondary structure

1151
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 17Combined sources12
Helixi20 – 34Combined sources15
Helixi36 – 41Combined sources6
Helixi44 – 46Combined sources3
Helixi52 – 55Combined sources4
Helixi59 – 77Combined sources19
Turni78 – 80Combined sources3
Beta strandi82 – 85Combined sources4
Helixi86 – 99Combined sources14
Helixi105 – 121Combined sources17
Helixi122 – 124Combined sources3
Helixi127 – 145Combined sources19
Helixi146 – 148Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q1FX-ray1.50A1-151[»]
1W92X-ray1.70A1-151[»]
2VRYX-ray1.87A1-151[»]
3GK9X-ray1.80A1-151[»]
3GKTX-ray1.86A1-151[»]
3GLNX-ray2.26A1-151[»]
4MU5X-ray1.80A1-151[»]
4NZIX-ray2.10A1-151[»]
4O1TX-ray1.60A1-151[»]
4O2GX-ray2.70A1-151[»]
4O35X-ray1.80A1-151[»]
4O4TX-ray1.90A1-151[»]
4O4ZX-ray1.70A1-151[»]
5EETX-ray2.00A3-150[»]
5EOHX-ray1.90A3-150[»]
5EQMX-ray2.05A3-150[»]
5EU2X-ray2.00A3-150[»]
5EV5X-ray2.00A3-150[»]
5EYJX-ray2.40A3-150[»]
5EYSX-ray1.75A3-150[»]
5F0BX-ray2.15A3-151[»]
5F2AX-ray2.10A1-151[»]
5MJCX-ray1.62A3-150[»]
5MJDX-ray1.70A3-150[»]
5NVIX-ray1.60A3-150[»]
5NW6X-ray1.70A3-150[»]
5O17X-ray1.80A3-150[»]
5O18X-ray1.86A3-150[»]
5O1KX-ray2.05A3-150[»]
5O27X-ray2.31A3-150[»]
6EYEX-ray1.70A1-151[»]
ProteinModelPortaliQ9ER97
SMRiQ9ER97
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ER97

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 149GlobinAdd BLAST149

Sequence similaritiesi

Belongs to the globin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3378 Eukaryota
COG1018 LUCA
GeneTreeiENSGT00510000048375
HOVERGENiHBG039321
InParanoidiQ9ER97
KOiK21893
PhylomeDBiQ9ER97
TreeFamiTF333247

Family and domain databases

Gene3Di1.10.490.10, 1 hit
InterProiView protein in InterPro
IPR000971 Globin
IPR009050 Globin-like_sf
IPR012292 Globin/Proto
PfamiView protein in Pfam
PF00042 Globin, 1 hit
SUPFAMiSSF46458 SSF46458, 1 hit
PROSITEiView protein in PROSITE
PS01033 GLOBIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ER97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERPESELIR QSWRVVSRSP LEHGTVLFAR LFALEPSLLP LFQYNGRQFS
60 70 80 90 100
SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLT SLGRKHRAVG
110 120 130 140 150
VRLSSFSTVG ESLLYMLEKC LGPDFTPATR TAWSRLYGAV VQAMSRGWDG

E
Length:151
Mass (Da):17,037
Last modified:March 1, 2001 - v1
Checksum:i377BBE4BF723CCF1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245945 mRNA Translation: CAC11135.1
BC024263 mRNA Translation: AAH24263.1
CCDSiCCDS36501.1
RefSeqiNP_071859.1, NM_022414.2
UniGeneiMm.41395

Genome annotation databases

EnsembliENSMUST00000021420; ENSMUSP00000021420; ENSMUSG00000021032
GeneIDi64242
KEGGimmu:64242
UCSCiuc007oii.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNGB_MOUSE
AccessioniPrimary (citable) accession number: Q9ER97
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health