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Protein

Cysteine--tRNA ligase, cytoplasmic

Gene

Cars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi138ZincBy similarity1
Metal bindingi431ZincBy similarity1
Metal bindingi456ZincBy similarity1
Metal bindingi460ZincBy similarity1
Binding sitei492ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine--tRNA ligase, cytoplasmic (EC:6.1.1.16)
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name:
CysRS
Gene namesi
Name:Cars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1351477. Cars.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001595512 – 831Cysteine--tRNA ligase, cytoplasmicAdd BLAST830

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei102PhosphoserineCombined sources1
Modified residuei388PhosphoserineBy similarity1
Modified residuei390PhosphoserineCombined sources1
Modified residuei829PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9ER72.
PaxDbiQ9ER72.
PeptideAtlasiQ9ER72.
PRIDEiQ9ER72.

PTM databases

iPTMnetiQ9ER72.
PhosphoSitePlusiQ9ER72.

Expressioni

Gene expression databases

BgeeiENSMUSG00000010755.
CleanExiMM_CARS.
ExpressionAtlasiQ9ER72. baseline and differential.
GenevisibleiQ9ER72. MM.

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9ER72. 1 interactor.
MINTiMINT-4136240.
STRINGi10090.ENSMUSP00000010899.

Structurei

3D structure databases

ProteinModelPortaliQ9ER72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi140 – 150"HIGH" regionAdd BLAST11
Motifi489 – 493"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2007. Eukaryota.
COG0215. LUCA.
GeneTreeiENSGT00390000006347.
HOVERGENiHBG002089.
InParanoidiQ9ER72.
KOiK01883.
OMAiFDILKLC.
OrthoDBiEOG091G03N1.
PhylomeDBiQ9ER72.
TreeFamiTF300384.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 2 hits.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR024909. Cys-tRNA/MSH_ligase.
IPR010987. Glutathione-S-Trfase_C-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ER72-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGSSAEQAA DYRSILSISD EAARVQALDQ HLSTRSYIQG YSLSQADVDV
60 70 80 90 100
FRQLSAPPAD SRLFHVARWF RHIEALLGGP QGRDEPCRLQ ASKGRRVQPQ
110 120 130 140 150
WSPPAGTEPC RLRLYNSLTR NKDVFIPQDG KKVTWYCCGP TVYDASHMGH
160 170 180 190 200
ARSYISFDIL RRVLRDYFQY DVFYCMNITD IDDKIIRRAR QNYLFEQYRE
210 220 230 240 250
QKPPATQLLK DVRDAMKPFS VKLSETTDPD KRQMLERIQN SVKLATEPLE
260 270 280 290 300
QAVRSSLSGE EVDSKVQVLL EEAKDLLSDW LDSTGGSEVT DNSIFSKLPK
310 320 330 340 350
FWEEEFHKDM EALNVLPPDV LTRVSEYVPE IVNFVQKIVD NGYGYASNGS
360 370 380 390 400
VYFDTAKFAA SEKHSYGKLV PEAVGDQKAL QEGEGDLSIS ADRLSEKRSP
410 420 430 440 450
NDFALWKASK PGEPSWPCPW GKGRPGWHIE CSAMAGTLLG ASMDIHGGGF
460 470 480 490 500
DLRFPHHDNE LAQSEAYFEN DCWVRYFLHT GHLTIAGCKM SKSLKNFITI
510 520 530 540 550
KDALKKHSAR QLRLAFLMHS WKDTLDYSSN TMESALQYEK FMNEFFLNVK
560 570 580 590 600
DILRAPVDIT GQFEKWEAEE VELNKNFYGK KTAVHEALCD NIDTRTVMEE
610 620 630 640 650
MRALVSQCNL YMAARKAERR RPNRALLENI AMYLTHMLKI FGAIEEESPL
660 670 680 690 700
GFPVGGPGTN LNLESTVMPY LQVLSEFREG VRKIAREKKV LEVLQLSDAL
710 720 730 740 750
RDDILPELGV RFEDHEGLPT VVKLVDRDTL LKEKEGKKRA EEEKRRKKEE
760 770 780 790 800
AARKKQEQEA AKLAKMKIPP SEMFLSEVNK YSKFDENGLP THDTEGKELS
810 820 830
KGQAKKLKKL FEAQEKLYKE YLQMLQNGSL Q
Length:831
Mass (Da):94,860
Last modified:May 10, 2004 - v2
Checksum:i5FE338D27AE32B92
GO
Isoform 2 (identifier: Q9ER72-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     9-92: AADYRSILSI...RDEPCRLQAS → G

Show »
Length:748
Mass (Da):85,551
Checksum:i48E21E753893755E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti161R → P in AAH30473 (PubMed:15489334).Curated1
Sequence conflicti164L → R in AAH30473 (PubMed:15489334).Curated1
Sequence conflicti310M → K in BAC28234 (PubMed:16141072).Curated1
Sequence conflicti313L → P in BAA29032 (Ref. 4) Curated1
Sequence conflicti742E → G in BAC28234 (PubMed:16141072).Curated1
Sequence conflicti771S → G in CAC16398 (PubMed:11063728).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0102589 – 92AADYR…RLQAS → G in isoform 2. 1 PublicationAdd BLAST84

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276505 Genomic DNA. Translation: CAC16398.1.
AJ276796 mRNA. Translation: CAC16403.1.
AK033328 mRNA. Translation: BAC28234.1.
AK037227 mRNA. Translation: BAC29766.1.
AK077558 mRNA. Translation: BAC36862.1.
BC030473 mRNA. Translation: AAH30473.1.
BC054717 mRNA. Translation: AAH54717.1.
BC058954 mRNA. Translation: AAH58954.1.
AB015589 mRNA. Translation: BAA29032.1.
CCDSiCCDS40198.1. [Q9ER72-1]
CCDS57597.1. [Q9ER72-2]
RefSeqiNP_001239522.1. NM_001252593.1. [Q9ER72-2]
NP_038770.3. NM_013742.5. [Q9ER72-1]
UniGeneiMm.125659.

Genome annotation databases

EnsembliENSMUST00000010899; ENSMUSP00000010899; ENSMUSG00000010755. [Q9ER72-1]
ENSMUST00000105909; ENSMUSP00000101529; ENSMUSG00000010755. [Q9ER72-2]
GeneIDi27267.
KEGGimmu:27267.
UCSCiuc009kpn.2. mouse. [Q9ER72-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276505 Genomic DNA. Translation: CAC16398.1.
AJ276796 mRNA. Translation: CAC16403.1.
AK033328 mRNA. Translation: BAC28234.1.
AK037227 mRNA. Translation: BAC29766.1.
AK077558 mRNA. Translation: BAC36862.1.
BC030473 mRNA. Translation: AAH30473.1.
BC054717 mRNA. Translation: AAH54717.1.
BC058954 mRNA. Translation: AAH58954.1.
AB015589 mRNA. Translation: BAA29032.1.
CCDSiCCDS40198.1. [Q9ER72-1]
CCDS57597.1. [Q9ER72-2]
RefSeqiNP_001239522.1. NM_001252593.1. [Q9ER72-2]
NP_038770.3. NM_013742.5. [Q9ER72-1]
UniGeneiMm.125659.

3D structure databases

ProteinModelPortaliQ9ER72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9ER72. 1 interactor.
MINTiMINT-4136240.
STRINGi10090.ENSMUSP00000010899.

PTM databases

iPTMnetiQ9ER72.
PhosphoSitePlusiQ9ER72.

Proteomic databases

EPDiQ9ER72.
PaxDbiQ9ER72.
PeptideAtlasiQ9ER72.
PRIDEiQ9ER72.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010899; ENSMUSP00000010899; ENSMUSG00000010755. [Q9ER72-1]
ENSMUST00000105909; ENSMUSP00000101529; ENSMUSG00000010755. [Q9ER72-2]
GeneIDi27267.
KEGGimmu:27267.
UCSCiuc009kpn.2. mouse. [Q9ER72-1]

Organism-specific databases

CTDi833.
MGIiMGI:1351477. Cars.

Phylogenomic databases

eggNOGiKOG2007. Eukaryota.
COG0215. LUCA.
GeneTreeiENSGT00390000006347.
HOVERGENiHBG002089.
InParanoidiQ9ER72.
KOiK01883.
OMAiFDILKLC.
OrthoDBiEOG091G03N1.
PhylomeDBiQ9ER72.
TreeFamiTF300384.

Miscellaneous databases

ChiTaRSiCars. mouse.
PROiQ9ER72.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000010755.
CleanExiMM_CARS.
ExpressionAtlasiQ9ER72. baseline and differential.
GenevisibleiQ9ER72. MM.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 2 hits.
HAMAPiMF_00041. Cys_tRNA_synth. 1 hit.
InterProiIPR015803. Cys-tRNA-ligase.
IPR024909. Cys-tRNA/MSH_ligase.
IPR010987. Glutathione-S-Trfase_C-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF47616. SSF47616. 1 hit.
TIGRFAMsiTIGR00435. cysS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYCC_MOUSE
AccessioniPrimary (citable) accession number: Q9ER72
Secondary accession number(s): O88303
, Q8BP81, Q8CAY7, Q8CCE3, Q8K0S4, Q9ER68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 10, 2004
Last modified: November 2, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.