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Protein

Rho-related GTP-binding protein RhoJ

Gene

Rhoj

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein with GTPase activity. Elicits the formation of F-actin-rich structures in fibroblasts and is involved in the regulation of cell morphology.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 35GTPBy similarity8
Nucleotide bindingi75 – 79GTPBy similarity5
Nucleotide bindingi133 – 136GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: MGI
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • actin cytoskeleton organization Source: UniProtKB
  • regulation of cell shape Source: MGI
  • retina vasculature morphogenesis in camera-type eye Source: MGI
  • Rho protein signal transduction Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell shape

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoJ
Alternative name(s):
Tc10-like GTP-binding protein
Gene namesi
Name:Rhoj
Synonyms:Arhj, Rhoi, Rhot, Tc10l, Tcl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1931551. Rhoj.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988701 – 211Rho-related GTP-binding protein RhoJAdd BLAST211
PropeptideiPRO_0000281221212 – 214Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei211Cysteine methyl esterBy similarity1
Lipidationi211S-farnesyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiQ9ER71.
PRIDEiQ9ER71.

PTM databases

iPTMnetiQ9ER71.
PhosphoSitePlusiQ9ER71.

Expressioni

Tissue specificityi

Highly expressed in heart with moderate levels in lung and liver. Very low levels detected in brain, spleen, skeletal muscle, kidney and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000046768.
CleanExiMM_RHOJ.
ExpressionAtlasiQ9ER71. baseline and differential.
GenevisibleiQ9ER71. MM.

Interactioni

Subunit structurei

Interacts with the CRIB domains of proteins such as Pak1 and Was/Wasp.1 Publication

Protein-protein interaction databases

BioGridi219817. 6 interactors.
STRINGi10090.ENSMUSP00000059498.

Structurei

3D structure databases

ProteinModelPortaliQ9ER71.
SMRiQ9ER71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 58Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118978.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiQ9ER71.
KOiK07864.
OMAiYILIGTQ.
OrthoDBiEOG091G0KCM.
PhylomeDBiQ9ER71.
TreeFamiTF101109.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ER71-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSCRERTDSS CGCNGHEENR ILKCVVVGDG AVGKTCLLMS YANDAFPEEY
60 70 80 90 100
VPTVFDHYAV TVTVGGKQHL LGLYDTAGQE DYNQLRPLSY PNTDVFLICF
110 120 130 140 150
SVVNPASYHN VQEEWVPELK DCMPHVPYVL IGTQIDLRDD PKTLARLLYM
160 170 180 190 200
KEKPLTYEHG VKLAKAIGAQ CYLECSALTQ KGLKAVFDEA ILTIFHPKKK
210
KKGCLGCHGC CAII
Length:214
Mass (Da):23,766
Last modified:October 25, 2002 - v2
Checksum:iA8070C73583A8AA3
GO
Isoform 2 (identifier: Q9ER71-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     4-13: Missing.

Note: No experimental confirmation available.
Show »
Length:204
Mass (Da):22,671
Checksum:i542A5E1AC93CF25F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti166A → R in AAL09441 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0057094 – 13Missing in isoform 2. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276568 mRNA. Translation: CAC06700.1.
AF309564 mRNA. Translation: AAL09441.1.
AB060651 mRNA. Translation: BAB91069.1.
AK003482 mRNA. Translation: BAB22812.1.
AK003490 mRNA. Translation: BAB22818.1.
AK156619 mRNA. Translation: BAE33778.1.
AK159385 mRNA. Translation: BAE35040.1.
BC043719 mRNA. Translation: AAH43719.1.
CCDSiCCDS25981.1. [Q9ER71-1]
RefSeqiNP_075764.1. NM_023275.2. [Q9ER71-1]
UniGeneiMm.27467.

Genome annotation databases

EnsembliENSMUST00000055390; ENSMUSP00000059498; ENSMUSG00000046768. [Q9ER71-1]
GeneIDi80837.
KEGGimmu:80837.
UCSCiuc007nxc.1. mouse. [Q9ER71-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276568 mRNA. Translation: CAC06700.1.
AF309564 mRNA. Translation: AAL09441.1.
AB060651 mRNA. Translation: BAB91069.1.
AK003482 mRNA. Translation: BAB22812.1.
AK003490 mRNA. Translation: BAB22818.1.
AK156619 mRNA. Translation: BAE33778.1.
AK159385 mRNA. Translation: BAE35040.1.
BC043719 mRNA. Translation: AAH43719.1.
CCDSiCCDS25981.1. [Q9ER71-1]
RefSeqiNP_075764.1. NM_023275.2. [Q9ER71-1]
UniGeneiMm.27467.

3D structure databases

ProteinModelPortaliQ9ER71.
SMRiQ9ER71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219817. 6 interactors.
STRINGi10090.ENSMUSP00000059498.

PTM databases

iPTMnetiQ9ER71.
PhosphoSitePlusiQ9ER71.

Proteomic databases

PaxDbiQ9ER71.
PRIDEiQ9ER71.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055390; ENSMUSP00000059498; ENSMUSG00000046768. [Q9ER71-1]
GeneIDi80837.
KEGGimmu:80837.
UCSCiuc007nxc.1. mouse. [Q9ER71-1]

Organism-specific databases

CTDi57381.
MGIiMGI:1931551. Rhoj.

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118978.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiQ9ER71.
KOiK07864.
OMAiYILIGTQ.
OrthoDBiEOG091G0KCM.
PhylomeDBiQ9ER71.
TreeFamiTF101109.

Enzyme and pathway databases

ReactomeiR-MMU-194840. Rho GTPase cycle.

Miscellaneous databases

PROiQ9ER71.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046768.
CleanExiMM_RHOJ.
ExpressionAtlasiQ9ER71. baseline and differential.
GenevisibleiQ9ER71. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHOJ_MOUSE
AccessioniPrimary (citable) accession number: Q9ER71
Secondary accession number(s): Q3TX76, Q920E4, Q9CQA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: October 25, 2002
Last modified: November 2, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.