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Protein

Oxysterol-binding protein-related protein 5

Gene

Osbpl5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner. May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes. Binds 25-hydroxycholesterol and cholesterol.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei448PhosphatidylserineBy similarity1
Binding sitei503PhosphatidylserineBy similarity1
Binding sitei669Phosphatidylinositol 4-phosphateBy similarity1
Binding sitei673Phosphatidylinositol 4-phosphateBy similarity1
Binding sitei677Phosphatidylinositol 4-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-1482801. Acyl chain remodelling of PS.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxysterol-binding protein-related protein 5
Short name:
ORP-5
Short name:
OSBP-related protein 5
Alternative name(s):
Oxysterol-binding protein homolog 11 Publication
Gene namesi
Name:Osbpl5
Synonyms:Obph11 Publication, Osbp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1930265. Osbpl5.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity

  • Note: Localizes to the cortical endoplasmic reticulum at the endoplasmic reticulum-plasma membrane contact sites.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei855 – 873HelicalSequence analysisAdd BLAST19

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001003741 – 874Oxysterol-binding protein-related protein 5Add BLAST874

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphoserineCombined sources1
Modified residuei292PhosphoserineBy similarity1
Modified residuei300PhosphoserineBy similarity1
Modified residuei746PhosphoserineCombined sources1
Modified residuei749PhosphoserineCombined sources1
Modified residuei776PhosphoserineBy similarity1
Modified residuei777PhosphoserineBy similarity1
Modified residuei779PhosphoserineBy similarity1
Modified residuei783PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9ER64.
MaxQBiQ9ER64.
PaxDbiQ9ER64.
PRIDEiQ9ER64.

PTM databases

iPTMnetiQ9ER64.
PhosphoSitePlusiQ9ER64.

Expressioni

Gene expression databases

BgeeiENSMUSG00000037606.
CleanExiMM_OSBP2.
MM_OSBPL5.
ExpressionAtlasiQ9ER64. baseline and differential.
GenevisibleiQ9ER64. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020411.

Structurei

3D structure databases

ProteinModelPortaliQ9ER64.
SMRiQ9ER64.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini126 – 243PHPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni383 – 388Phosphatidylinositol 4-phosphate bindingBy similarity6
Regioni383 – 388Phosphatidylserine bindingBy similarity6
Regioni445 – 448Phosphatidylinositol 4-phosphate bindingBy similarity4
Regioni477 – 478Phosphatidylinositol 4-phosphate bindingBy similarity2

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili93 – 123Sequence analysisAdd BLAST31

Sequence similaritiesi

Belongs to the OSBP family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2210. Eukaryota.
ENOG410XRW6. LUCA.
GeneTreeiENSGT00550000074515.
HOGENOMiHOG000233870.
HOVERGENiHBG053375.
InParanoidiQ9ER64.
KOiK20464.
PhylomeDBiQ9ER64.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000648. Oxysterol-bd.
IPR018494. Oxysterol-bd_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR10972. PTHR10972. 1 hit.
PfamiPF01237. Oxysterol_BP. 2 hits.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS01013. OSBP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ER64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEEAFLRRR FSLCPPASTP QKTDPRKVPR NLLLGCENEL GPITPGRDME
60 70 80 90 100
SNGPSQPRDE EPQTPGSATK VPLAEYRLCN GSDKECTSPT TRVSKKDALK
110 120 130 140 150
AQKENYRQEK KRATKQLFSA LTDPSVVIMA DSLKIRGTLK SWTKLWCVLK
160 170 180 190 200
PGVLLIYKTP KVGQWVGTVL LHCCELIERP SKKDGFCFKL FHPLDQSVWA
210 220 230 240 250
VKGPKGESVG SITQPLPSSY LIFRAASESD GRCWLDALEL ALRCSSLLRL
260 270 280 290 300
STCKQGRDGE QGSSPDASPS SLYGLPTSAT IPDQDLFPLN GSALENDAFS
310 320 330 340 350
DKSERENAED SDAETQDHSR KTNESGSDLL DSPGGPWRGT TYVEQVQEEL
360 370 380 390 400
GELDETSQVE TVSEENKSLM WVLLRQLRPG MDLSRVVLPT FVLEPRSFLG
410 420 430 440 450
KLSDYYYHGD LLSRAAAEDD PYCRMKLVLR WYLSGFYKKP KGIKKPYNPI
460 470 480 490 500
LGETFRCRWL HPQTNSHTFY IAEQVSHHPP VSAFYVSNRK DGFCMSGSIT
510 520 530 540 550
AKSKFYGNSL SALLDGKAKL TFLNRKEEYT LTMPYAHCRG ILYGTMTMEL
560 570 580 590 600
GGKVNIECEK NNLQAELDFK LKPFFGSSAN INQISGKIMS GEEVLARLTG
610 620 630 640 650
HWDRDVFIKE ESSGGTELFW TPSEEVRRQR LKRHTVLLEE QSELESERLW
660 670 680 690 700
QHVTRAIREG DQHKATQEKS VLEEAQRQRA REHQQSLTPW KPQLFLLDPL
710 720 730 740 750
TQEWRYRYED LSPWDPLKDI AQYEQDGILH TLQRETMSGQ TTFLGSPDSR
760 770 780 790 800
HKRPSSDRRL RKASDQPSGH SQVTESSGST PESCPDLSDE DFVPGGESPC
810 820 830 840 850
PRCRREVHRL KMLQEAVLSI QEAQQELHRH LSTMLSSTVR AGQAPAPSLL
860 870
QNPRSWFLLC IFLTCQLFIN YILK
Length:874
Mass (Da):98,922
Last modified:February 11, 2002 - v3
Checksum:iFBC41FA8E219F5E3
GO

Sequence cautioni

The sequence CAC16404 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAC27351 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37 – 44ENELGPIT → MSLVPSPQ in CAC27351 (PubMed:11063728).Curated8
Sequence conflicti312D → N in BAB85687 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278263 mRNA. Translation: CAC16404.2. Different initiation.
AJ276505 Genomic DNA. Translation: CAC27351.1. Different initiation.
AB074008 mRNA. Translation: BAB85687.1.
BC079872 mRNA. Translation: AAH79872.1.
CCDSiCCDS57598.1.
RefSeqiNP_001186156.1. NM_001199227.1.
UniGeneiMm.21199.

Genome annotation databases

EnsembliENSMUST00000119499; ENSMUSP00000113362; ENSMUSG00000037606.
GeneIDi79196.
KEGGimmu:79196.
UCSCiuc009kpw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278263 mRNA. Translation: CAC16404.2. Different initiation.
AJ276505 Genomic DNA. Translation: CAC27351.1. Different initiation.
AB074008 mRNA. Translation: BAB85687.1.
BC079872 mRNA. Translation: AAH79872.1.
CCDSiCCDS57598.1.
RefSeqiNP_001186156.1. NM_001199227.1.
UniGeneiMm.21199.

3D structure databases

ProteinModelPortaliQ9ER64.
SMRiQ9ER64.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020411.

PTM databases

iPTMnetiQ9ER64.
PhosphoSitePlusiQ9ER64.

Proteomic databases

EPDiQ9ER64.
MaxQBiQ9ER64.
PaxDbiQ9ER64.
PRIDEiQ9ER64.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000119499; ENSMUSP00000113362; ENSMUSG00000037606.
GeneIDi79196.
KEGGimmu:79196.
UCSCiuc009kpw.2. mouse.

Organism-specific databases

CTDi114879.
MGIiMGI:1930265. Osbpl5.

Phylogenomic databases

eggNOGiKOG2210. Eukaryota.
ENOG410XRW6. LUCA.
GeneTreeiENSGT00550000074515.
HOGENOMiHOG000233870.
HOVERGENiHBG053375.
InParanoidiQ9ER64.
KOiK20464.
PhylomeDBiQ9ER64.

Enzyme and pathway databases

ReactomeiR-MMU-1482801. Acyl chain remodelling of PS.

Miscellaneous databases

ChiTaRSiOsbpl5. mouse.
PROiQ9ER64.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037606.
CleanExiMM_OSBP2.
MM_OSBPL5.
ExpressionAtlasiQ9ER64. baseline and differential.
GenevisibleiQ9ER64. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR000648. Oxysterol-bd.
IPR018494. Oxysterol-bd_CS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PANTHERiPTHR10972. PTHR10972. 1 hit.
PfamiPF01237. Oxysterol_BP. 2 hits.
PF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS01013. OSBP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOSBL5_MOUSE
AccessioniPrimary (citable) accession number: Q9ER64
Secondary accession number(s): Q8R510, Q99NF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: February 11, 2002
Last modified: November 30, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Imprinted gene expressed from the maternal allele in blastocysts.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.