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Q9ER60

- SCN4A_MOUSE

UniProt

Q9ER60 - SCN4A_MOUSE

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Protein

Sodium channel protein type 4 subunit alpha

Gene

Scn4a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This sodium channel may be present in both denervated and innervated skeletal muscle.

GO - Molecular functioni

  1. voltage-gated sodium channel activity Source: MGI

GO - Biological processi

  1. membrane depolarization during action potential Source: RefGenome
  2. neuronal action potential Source: RefGenome
  3. sodium ion transmembrane transport Source: GOC
  4. sodium ion transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Sodium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium channel protein type 4 subunit alpha
Alternative name(s):
Sodium channel protein skeletal muscle subunit alpha
Sodium channel protein type IV subunit alpha
Voltage-gated sodium channel subunit alpha Nav1.4
Gene namesi
Name:Scn4a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:98250. Scn4a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 128128CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei129 – 15022Helical; Name=S1 of repeat ISequence AnalysisAdd
BLAST
Topological domaini151 – 1588ExtracellularSequence Analysis
Transmembranei159 – 17820Helical; Name=S2 of repeat ISequence AnalysisAdd
BLAST
Topological domaini179 – 19012CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei191 – 21020Helical; Name=S3 of repeat ISequence AnalysisAdd
BLAST
Topological domaini211 – 2166ExtracellularSequence Analysis
Transmembranei217 – 23620Helical; Voltage-sensor; Name=S4 of repeat ISequence AnalysisAdd
BLAST
Topological domaini237 – 25216CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei253 – 26614Helical; Name=S5 of repeat ISequence AnalysisAdd
BLAST
Topological domaini267 – 417151ExtracellularSequence AnalysisAdd
BLAST
Transmembranei418 – 44326Helical; Name=S6 of repeat ISequence AnalysisAdd
BLAST
Topological domaini444 – 567124CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei568 – 59124Helical; Name=S1 of repeat IISequence AnalysisAdd
BLAST
Topological domaini592 – 60211ExtracellularSequence AnalysisAdd
BLAST
Transmembranei603 – 62624Helical; Name=S2 of repeat IISequence AnalysisAdd
BLAST
Topological domaini627 – 6348CytoplasmicSequence Analysis
Transmembranei635 – 65420Helical; Name=S3 of repeat IISequence AnalysisAdd
BLAST
Topological domaini655 – 6606ExtracellularSequence Analysis
Transmembranei661 – 68020Helical; Voltage-sensor; Name=S4 of repeat IISequence AnalysisAdd
BLAST
Topological domaini681 – 69515CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei696 – 71823Helical; Name=S5 of repeat IISequence AnalysisAdd
BLAST
Topological domaini719 – 77052ExtracellularSequence AnalysisAdd
BLAST
Transmembranei771 – 79626Helical; Name=S6 of repeat IISequence AnalysisAdd
BLAST
Topological domaini797 – 1020224CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1021 – 104323Helical; Name=S1 of repeat IIISequence AnalysisAdd
BLAST
Topological domaini1044 – 105714ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1058 – 108326Helical; Name=S2 of repeat IIISequence AnalysisAdd
BLAST
Topological domaini1084 – 10896CytoplasmicSequence Analysis
Transmembranei1090 – 111021Helical; Name=S3 of repeat IIISequence AnalysisAdd
BLAST
Topological domaini1111 – 11155ExtracellularSequence Analysis
Transmembranei1116 – 113722Helical; Voltage-sensor; Name=S4 of repeat IIISequence AnalysisAdd
BLAST
Topological domaini1138 – 115619CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1157 – 117822Helical; Name=S5 of repeat IIISequence AnalysisAdd
BLAST
Topological domaini1179 – 126284ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1263 – 128927Helical; Name=S6 of repeat IIISequence AnalysisAdd
BLAST
Topological domaini1290 – 134253CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1343 – 136624Helical; Name=S1 of repeat IVSequence AnalysisAdd
BLAST
Topological domaini1367 – 137711ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1378 – 140124Helical; Name=S2 of repeat IVSequence AnalysisAdd
BLAST
Topological domaini1402 – 14076CytoplasmicSequence Analysis
Transmembranei1408 – 143124Helical; Name=S3 of repeat IVSequence AnalysisAdd
BLAST
Topological domaini1432 – 14409ExtracellularSequence Analysis
Transmembranei1441 – 146323Helical; Voltage-sensor; Name=S4 of repeat IVSequence AnalysisAdd
BLAST
Topological domaini1464 – 147815CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei1479 – 150123Helical; Name=S5 of repeat IVSequence AnalysisAdd
BLAST
Topological domaini1502 – 156766ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1568 – 159225Helical; Name=S6 of repeat IVSequence AnalysisAdd
BLAST
Topological domaini1593 – 1841249CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. plasma membrane Source: RefGenome
  2. voltage-gated sodium channel complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18411841Sodium channel protein type 4 subunit alphaPRO_0000371316Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi214 – 2141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi291 – 2911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1185 – 11851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1199 – 11991N-linked (GlcNAc...)Sequence Analysis
Modified residuei1322 – 13221Phosphoserine; by PKCBy similarity

Post-translational modificationi

Phosphorylation at Ser-1322 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9ER60.
PaxDbiQ9ER60.
PRIDEiQ9ER60.

PTM databases

PhosphoSiteiQ9ER60.

Expressioni

Tissue specificityi

Expressed in the myocardium.1 Publication

Gene expression databases

GenevestigatoriQ9ER60.

Interactioni

Subunit structurei

Muscle sodium channels contain an alpha subunit and a smaller beta subunit. Interacts with the PDZ domain of the syntrophin SNTA1, SNTB1 and SNTB2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102431.

Structurei

3D structure databases

ProteinModelPortaliQ9ER60.
SMRiQ9ER60. Positions 131-272, 582-796, 1021-1290, 1349-1748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati128 – 444317IAdd
BLAST
Repeati565 – 790226IIAdd
BLAST
Repeati1018 – 1275258IIIAdd
BLAST
Repeati1343 – 1589247IVAdd
BLAST
Domaini1721 – 175030IQPROSITE-ProRule annotationAdd
BLAST

Domaini

The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1226.
HOGENOMiHOG000231755.
InParanoidiQ9ER60.
KOiK04837.
PhylomeDBiQ9ER60.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR028826. Na_channel_a4su.
IPR008052. Na_channel_a4su_mammal.
IPR001696. Na_channel_asu.
IPR010526. Na_trans_assoc.
[Graphical view]
PANTHERiPTHR10037:SF193. PTHR10037:SF193. 1 hit.
PfamiPF00520. Ion_trans. 4 hits.
PF06512. Na_trans_assoc. 1 hit.
[Graphical view]
PRINTSiPR00170. NACHANNEL.
PR01665. NACHANNEL4.
SMARTiSM00015. IQ. 1 hit.
[Graphical view]
PROSITEiPS50096. IQ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ER60-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASSSLPTLV PPGPHCLRPF TPESLAAIEQ RAMEEEARLQ RNKQMEIEEP
60 70 80 90 100
ERKPRSDLEA GKNLPLIYGD PPPEVIGVPL EDLDPYYSDK KTFIVLNKGK
110 120 130 140 150
AIFRFSATPA LYMLSPFSIV RRVAIKVLIH ALFSMFIMIT ILTNCVFMTM
160 170 180 190 200
SNPPSWSKDV EYTFTGIYTF ESLIKMLARG FCIDDFTFLR DPWNWLDFSV
210 220 230 240 250
ITMAYVTEFV DLGNISALRT FRVLRALKTI TVIPGLKTIV GALIQSVKKL
260 270 280 290 300
SDVMILTVFC LSVFALVGLQ LFMGNLRQKC VRWPPPMNDT NTTWYGNDTW
310 320 330 340 350
YGNDTWYGND TWYGNDTWNS QESWVSNSTF DWEAYINDEG NFYFLEGSND
360 370 380 390 400
ALLCGNSSDA GHCPEGYECM KAGRNPNYGY TSYDTFSWAF LALFRLMTQD
410 420 430 440 450
YWENLFQLTL RAAGKTYMIF FVVIIFLGSF YLINLILAVV AMAYAEQNEA
460 470 480 490 500
TLAEDQEKEE EFQQMLEKFK KHQEELEKAK AAQALEGGEE ADGDPTHSKD
510 520 530 540 550
CNGSLDTSGE KGPPRPSCSA ESAISDAMEE LEEAHQKCPP WWYKCAHKVL
560 570 580 590 600
IWNCCAPWVK FKHIILLIVM DPFVDLGITI CIVLNTLFMA MEHYPMTEHF
610 620 630 640 650
DNVLSVGNLV FTGIFTAEMV LKLIAMDPYE YFQQGWNIFD SFIVTLSLVE
660 670 680 690 700
LGLANVQGLS VLRSFRLLRV FKLAKSWPTL NMLIKIIGNS VGALGNLTLV
710 720 730 740 750
LAIIVFIFAV VGMQLFGKSY KECVCKIASD CSLPRWHMHD FFHSFLIVFR
760 770 780 790 800
ILCGEWIETM WDCMEVAGQA MCLTVFLMVM VIGNLVVLNL FLALLLSSFS
810 820 830 840 850
ADSLAASDED GEMNNLQIAI GRIKWGIAFA KTFLLGLLHG KILSLKDIML
860 870 880 890 900
SLGEPGGAGE NGESPPEDEK KEPPPEDGNK ELKDNHILNH VGLTDGPRSS
910 920 930 940 950
IEMDHLNFIN NPYLTIHVPI ASEESDLEMP TEEETDTFSE PEDIKKPLQP
960 970 980 990 1000
LYDGNSSVCS TADYKPPEED PEEQAEENPE GELPEECFTE ACVKRCPCLY
1010 1020 1030 1040 1050
VDISQGRGKM WWTLRRACFK IVEHNWFETF IVFMILLSSG ALAFEDIYIE
1060 1070 1080 1090 1100
QRRVIRTILE YADKVFTYIF ILEMLLKWVA YGFKVYFTNA WCWLDFLIVD
1110 1120 1130 1140 1150
VSIISLVANW LGYSELGPIK SLRTLRALRP LRALSRFEGM RVVVNALLGA
1160 1170 1180 1190 1200
IPSIMNVLLV CLIFWLIFSI MGVNLFAGKF YYCINTTTSE RFDISVVNNK
1210 1220 1230 1240 1250
SECESLMYTG QVRWMNVKVN YDNVGLGYLS LLQVATFKGW MDIMYAAVDS
1260 1270 1280 1290 1300
REKEEQPDYE VNLYMYLYFV IFIIFGSFFT LNLFIGVIID NFNQQKKKFG
1310 1320 1330 1340 1350
GKDIFMTEEQ KKYYNAMKKL GSKKPQKPIP RPQNKIQGMV YDFVTKQVFD
1360 1370 1380 1390 1400
ISIMILICLN MVTMMVETDD QSQLKVDILY NINMVFIIVF TGECVLKMFA
1410 1420 1430 1440 1450
LRHYYFTIGW NIFDFVVVIL SIVGLALSDL IQKYFVSPTL FRVIRLARIG
1460 1470 1480 1490 1500
RVLRLIRGAK GIRTLLFALM MSLPALFNIG LLLFLVMFIY SIFGMSNFAY
1510 1520 1530 1540 1550
VKKESGIDDM FNFETFGNSI ICLFEITTSA GWDGLLNPIL NSGPPDCDPT
1560 1570 1580 1590 1600
LENPGTNIKG DCGNPSIGIC FFCSYIIISF LIVVNMYIAI ILENFNVATE
1610 1620 1630 1640 1650
ESSEPLCEDD FEMFYETWEK FDPDATQFID YSRLSDFVDT LQEPLKIAKP
1660 1670 1680 1690 1700
NKIKLITLDL PMVPGDKIHC LDILFALTKE VLGDSGEMDA LKQTMEEKFM
1710 1720 1730 1740 1750
AANPSKVSYE PITTTLKRKQ EEVCAIKIQR AYRRHLLQRS VKQASYMYRH
1760 1770 1780 1790 1800
SQEGNGDGAP EKEGLLANTM NKMYGSEKED NGVQSQGEKE KDSTEDAGPT
1810 1820 1830 1840
TEVTAPSSSD TALTPPPPSP PPPSSPPQGQ TVRPGVKESL V
Length:1,841
Mass (Da):208,798
Last modified:March 1, 2001 - v1
Checksum:i0766DFD33A9E0E55
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti131 – 1311A → T in CAM23795. (PubMed:19468303)Curated
Sequence conflicti1056 – 10561R → Q in CAM23795. (PubMed:19468303)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278787 mRNA. Translation: CAC17146.1.
AL604045 Genomic DNA. Translation: CAM23795.1.
BC129805 mRNA. Translation: AAI29806.1.
CCDSiCCDS48961.1.
RefSeqiNP_573462.2. NM_133199.2.
UniGeneiMm.432528.

Genome annotation databases

GeneIDi110880.
KEGGimmu:110880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ278787 mRNA. Translation: CAC17146.1 .
AL604045 Genomic DNA. Translation: CAM23795.1 .
BC129805 mRNA. Translation: AAI29806.1 .
CCDSi CCDS48961.1.
RefSeqi NP_573462.2. NM_133199.2.
UniGenei Mm.432528.

3D structure databases

ProteinModelPortali Q9ER60.
SMRi Q9ER60. Positions 131-272, 582-796, 1021-1290, 1349-1748.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000102431.

PTM databases

PhosphoSitei Q9ER60.

Proteomic databases

MaxQBi Q9ER60.
PaxDbi Q9ER60.
PRIDEi Q9ER60.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 110880.
KEGGi mmu:110880.

Organism-specific databases

CTDi 6329.
MGIi MGI:98250. Scn4a.

Phylogenomic databases

eggNOGi COG1226.
HOGENOMi HOG000231755.
InParanoidi Q9ER60.
KOi K04837.
PhylomeDBi Q9ER60.

Miscellaneous databases

NextBioi 364857.
PROi Q9ER60.
SOURCEi Search...

Gene expression databases

Genevestigatori Q9ER60.

Family and domain databases

Gene3Di 1.20.120.350. 4 hits.
InterProi IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR028826. Na_channel_a4su.
IPR008052. Na_channel_a4su_mammal.
IPR001696. Na_channel_asu.
IPR010526. Na_trans_assoc.
[Graphical view ]
PANTHERi PTHR10037:SF193. PTHR10037:SF193. 1 hit.
Pfami PF00520. Ion_trans. 4 hits.
PF06512. Na_trans_assoc. 1 hit.
[Graphical view ]
PRINTSi PR00170. NACHANNEL.
PR01665. NACHANNEL4.
SMARTi SM00015. IQ. 1 hit.
[Graphical view ]
PROSITEi PS50096. IQ. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse heart Na+ channels: primary structure and function of two isoforms and alternatively spliced variants."
    Zimmer T., Bollensdorff C., Haufe V., Birch-Hirschfeld E., Benndorf K.
    Am. J. Physiol. 282:H1007-H1017(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Heart.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-1841.

Entry informationi

Entry nameiSCN4A_MOUSE
AccessioniPrimary (citable) accession number: Q9ER60
Secondary accession number(s): A2VDE9, B1ARK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2001
Last modified: November 26, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3