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Q9ER60

- SCN4A_MOUSE

UniProt

Q9ER60 - SCN4A_MOUSE

Protein

Sodium channel protein type 4 subunit alpha

Gene

Scn4a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This sodium channel may be present in both denervated and innervated skeletal muscle.

    GO - Molecular functioni

    1. voltage-gated sodium channel activity Source: MGI

    GO - Biological processi

    1. membrane depolarization during action potential Source: RefGenome
    2. neuronal action potential Source: RefGenome
    3. sodium ion transmembrane transport Source: GOC
    4. sodium ion transport Source: MGI

    Keywords - Molecular functioni

    Ion channel, Sodium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium channel protein type 4 subunit alpha
    Alternative name(s):
    Sodium channel protein skeletal muscle subunit alpha
    Sodium channel protein type IV subunit alpha
    Voltage-gated sodium channel subunit alpha Nav1.4
    Gene namesi
    Name:Scn4a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:98250. Scn4a.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: RefGenome
    2. voltage-gated sodium channel complex Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 18411841Sodium channel protein type 4 subunit alphaPRO_0000371316Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi214 – 2141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi291 – 2911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi327 – 3271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1185 – 11851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1199 – 11991N-linked (GlcNAc...)Sequence Analysis
    Modified residuei1322 – 13221Phosphoserine; by PKCBy similarity

    Post-translational modificationi

    Phosphorylation at Ser-1322 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ9ER60.
    PRIDEiQ9ER60.

    PTM databases

    PhosphoSiteiQ9ER60.

    Expressioni

    Tissue specificityi

    Expressed in the myocardium.1 Publication

    Gene expression databases

    GenevestigatoriQ9ER60.

    Interactioni

    Subunit structurei

    Muscle sodium channels contain an alpha subunit and a smaller beta subunit. Interacts with the PDZ domain of the syntrophin SNTA1, SNTB1 and SNTB2 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000102431.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ER60.
    SMRiQ9ER60. Positions 131-272, 582-796, 1014-1290, 1349-1748.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 128128CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini151 – 1588ExtracellularSequence Analysis
    Topological domaini179 – 19012CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini211 – 2166ExtracellularSequence Analysis
    Topological domaini237 – 25216CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini267 – 417151ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini444 – 567124CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini592 – 60211ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini627 – 6348CytoplasmicSequence Analysis
    Topological domaini655 – 6606ExtracellularSequence Analysis
    Topological domaini681 – 69515CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini719 – 77052ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini797 – 1020224CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1044 – 105714ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1084 – 10896CytoplasmicSequence Analysis
    Topological domaini1111 – 11155ExtracellularSequence Analysis
    Topological domaini1138 – 115619CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1179 – 126284ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1290 – 134253CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1367 – 137711ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1402 – 14076CytoplasmicSequence Analysis
    Topological domaini1432 – 14409ExtracellularSequence Analysis
    Topological domaini1464 – 147815CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1502 – 156766ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1593 – 1841249CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei129 – 15022Helical; Name=S1 of repeat ISequence AnalysisAdd
    BLAST
    Transmembranei159 – 17820Helical; Name=S2 of repeat ISequence AnalysisAdd
    BLAST
    Transmembranei191 – 21020Helical; Name=S3 of repeat ISequence AnalysisAdd
    BLAST
    Transmembranei217 – 23620Helical; Voltage-sensor; Name=S4 of repeat ISequence AnalysisAdd
    BLAST
    Transmembranei253 – 26614Helical; Name=S5 of repeat ISequence AnalysisAdd
    BLAST
    Transmembranei418 – 44326Helical; Name=S6 of repeat ISequence AnalysisAdd
    BLAST
    Transmembranei568 – 59124Helical; Name=S1 of repeat IISequence AnalysisAdd
    BLAST
    Transmembranei603 – 62624Helical; Name=S2 of repeat IISequence AnalysisAdd
    BLAST
    Transmembranei635 – 65420Helical; Name=S3 of repeat IISequence AnalysisAdd
    BLAST
    Transmembranei661 – 68020Helical; Voltage-sensor; Name=S4 of repeat IISequence AnalysisAdd
    BLAST
    Transmembranei696 – 71823Helical; Name=S5 of repeat IISequence AnalysisAdd
    BLAST
    Transmembranei771 – 79626Helical; Name=S6 of repeat IISequence AnalysisAdd
    BLAST
    Transmembranei1021 – 104323Helical; Name=S1 of repeat IIISequence AnalysisAdd
    BLAST
    Transmembranei1058 – 108326Helical; Name=S2 of repeat IIISequence AnalysisAdd
    BLAST
    Transmembranei1090 – 111021Helical; Name=S3 of repeat IIISequence AnalysisAdd
    BLAST
    Transmembranei1116 – 113722Helical; Voltage-sensor; Name=S4 of repeat IIISequence AnalysisAdd
    BLAST
    Transmembranei1157 – 117822Helical; Name=S5 of repeat IIISequence AnalysisAdd
    BLAST
    Transmembranei1263 – 128927Helical; Name=S6 of repeat IIISequence AnalysisAdd
    BLAST
    Transmembranei1343 – 136624Helical; Name=S1 of repeat IVSequence AnalysisAdd
    BLAST
    Transmembranei1378 – 140124Helical; Name=S2 of repeat IVSequence AnalysisAdd
    BLAST
    Transmembranei1408 – 143124Helical; Name=S3 of repeat IVSequence AnalysisAdd
    BLAST
    Transmembranei1441 – 146323Helical; Voltage-sensor; Name=S4 of repeat IVSequence AnalysisAdd
    BLAST
    Transmembranei1479 – 150123Helical; Name=S5 of repeat IVSequence AnalysisAdd
    BLAST
    Transmembranei1568 – 159225Helical; Name=S6 of repeat IVSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati128 – 444317IAdd
    BLAST
    Repeati565 – 790226IIAdd
    BLAST
    Repeati1018 – 1275258IIIAdd
    BLAST
    Repeati1343 – 1589247IVAdd
    BLAST
    Domaini1721 – 175030IQPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Contains 1 IQ domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    HOGENOMiHOG000231755.
    InParanoidiB1ARK0.
    KOiK04837.
    PhylomeDBiQ9ER60.

    Family and domain databases

    Gene3Di1.20.120.350. 4 hits.
    InterProiIPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR028826. Na_channel_a4su.
    IPR008052. Na_channel_a4su_mammal.
    IPR001696. Na_channel_asu.
    IPR010526. Na_trans_assoc.
    [Graphical view]
    PANTHERiPTHR10037:SF193. PTHR10037:SF193. 1 hit.
    PfamiPF00520. Ion_trans. 4 hits.
    PF06512. Na_trans_assoc. 1 hit.
    [Graphical view]
    PRINTSiPR00170. NACHANNEL.
    PR01665. NACHANNEL4.
    SMARTiSM00015. IQ. 1 hit.
    [Graphical view]
    PROSITEiPS50096. IQ. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9ER60-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASSSLPTLV PPGPHCLRPF TPESLAAIEQ RAMEEEARLQ RNKQMEIEEP     50
    ERKPRSDLEA GKNLPLIYGD PPPEVIGVPL EDLDPYYSDK KTFIVLNKGK 100
    AIFRFSATPA LYMLSPFSIV RRVAIKVLIH ALFSMFIMIT ILTNCVFMTM 150
    SNPPSWSKDV EYTFTGIYTF ESLIKMLARG FCIDDFTFLR DPWNWLDFSV 200
    ITMAYVTEFV DLGNISALRT FRVLRALKTI TVIPGLKTIV GALIQSVKKL 250
    SDVMILTVFC LSVFALVGLQ LFMGNLRQKC VRWPPPMNDT NTTWYGNDTW 300
    YGNDTWYGND TWYGNDTWNS QESWVSNSTF DWEAYINDEG NFYFLEGSND 350
    ALLCGNSSDA GHCPEGYECM KAGRNPNYGY TSYDTFSWAF LALFRLMTQD 400
    YWENLFQLTL RAAGKTYMIF FVVIIFLGSF YLINLILAVV AMAYAEQNEA 450
    TLAEDQEKEE EFQQMLEKFK KHQEELEKAK AAQALEGGEE ADGDPTHSKD 500
    CNGSLDTSGE KGPPRPSCSA ESAISDAMEE LEEAHQKCPP WWYKCAHKVL 550
    IWNCCAPWVK FKHIILLIVM DPFVDLGITI CIVLNTLFMA MEHYPMTEHF 600
    DNVLSVGNLV FTGIFTAEMV LKLIAMDPYE YFQQGWNIFD SFIVTLSLVE 650
    LGLANVQGLS VLRSFRLLRV FKLAKSWPTL NMLIKIIGNS VGALGNLTLV 700
    LAIIVFIFAV VGMQLFGKSY KECVCKIASD CSLPRWHMHD FFHSFLIVFR 750
    ILCGEWIETM WDCMEVAGQA MCLTVFLMVM VIGNLVVLNL FLALLLSSFS 800
    ADSLAASDED GEMNNLQIAI GRIKWGIAFA KTFLLGLLHG KILSLKDIML 850
    SLGEPGGAGE NGESPPEDEK KEPPPEDGNK ELKDNHILNH VGLTDGPRSS 900
    IEMDHLNFIN NPYLTIHVPI ASEESDLEMP TEEETDTFSE PEDIKKPLQP 950
    LYDGNSSVCS TADYKPPEED PEEQAEENPE GELPEECFTE ACVKRCPCLY 1000
    VDISQGRGKM WWTLRRACFK IVEHNWFETF IVFMILLSSG ALAFEDIYIE 1050
    QRRVIRTILE YADKVFTYIF ILEMLLKWVA YGFKVYFTNA WCWLDFLIVD 1100
    VSIISLVANW LGYSELGPIK SLRTLRALRP LRALSRFEGM RVVVNALLGA 1150
    IPSIMNVLLV CLIFWLIFSI MGVNLFAGKF YYCINTTTSE RFDISVVNNK 1200
    SECESLMYTG QVRWMNVKVN YDNVGLGYLS LLQVATFKGW MDIMYAAVDS 1250
    REKEEQPDYE VNLYMYLYFV IFIIFGSFFT LNLFIGVIID NFNQQKKKFG 1300
    GKDIFMTEEQ KKYYNAMKKL GSKKPQKPIP RPQNKIQGMV YDFVTKQVFD 1350
    ISIMILICLN MVTMMVETDD QSQLKVDILY NINMVFIIVF TGECVLKMFA 1400
    LRHYYFTIGW NIFDFVVVIL SIVGLALSDL IQKYFVSPTL FRVIRLARIG 1450
    RVLRLIRGAK GIRTLLFALM MSLPALFNIG LLLFLVMFIY SIFGMSNFAY 1500
    VKKESGIDDM FNFETFGNSI ICLFEITTSA GWDGLLNPIL NSGPPDCDPT 1550
    LENPGTNIKG DCGNPSIGIC FFCSYIIISF LIVVNMYIAI ILENFNVATE 1600
    ESSEPLCEDD FEMFYETWEK FDPDATQFID YSRLSDFVDT LQEPLKIAKP 1650
    NKIKLITLDL PMVPGDKIHC LDILFALTKE VLGDSGEMDA LKQTMEEKFM 1700
    AANPSKVSYE PITTTLKRKQ EEVCAIKIQR AYRRHLLQRS VKQASYMYRH 1750
    SQEGNGDGAP EKEGLLANTM NKMYGSEKED NGVQSQGEKE KDSTEDAGPT 1800
    TEVTAPSSSD TALTPPPPSP PPPSSPPQGQ TVRPGVKESL V 1841
    Length:1,841
    Mass (Da):208,798
    Last modified:March 1, 2001 - v1
    Checksum:i0766DFD33A9E0E55
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti131 – 1311A → T in CAM23795. (PubMed:19468303)Curated
    Sequence conflicti1056 – 10561R → Q in CAM23795. (PubMed:19468303)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ278787 mRNA. Translation: CAC17146.1.
    AL604045 Genomic DNA. Translation: CAM23795.1.
    BC129805 mRNA. Translation: AAI29806.1.
    CCDSiCCDS48961.1.
    RefSeqiNP_573462.2. NM_133199.2.
    UniGeneiMm.432528.

    Genome annotation databases

    GeneIDi110880.
    KEGGimmu:110880.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ278787 mRNA. Translation: CAC17146.1 .
    AL604045 Genomic DNA. Translation: CAM23795.1 .
    BC129805 mRNA. Translation: AAI29806.1 .
    CCDSi CCDS48961.1.
    RefSeqi NP_573462.2. NM_133199.2.
    UniGenei Mm.432528.

    3D structure databases

    ProteinModelPortali Q9ER60.
    SMRi Q9ER60. Positions 131-272, 582-796, 1014-1290, 1349-1748.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000102431.

    PTM databases

    PhosphoSitei Q9ER60.

    Proteomic databases

    PaxDbi Q9ER60.
    PRIDEi Q9ER60.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 110880.
    KEGGi mmu:110880.

    Organism-specific databases

    CTDi 6329.
    MGIi MGI:98250. Scn4a.

    Phylogenomic databases

    eggNOGi COG1226.
    HOGENOMi HOG000231755.
    InParanoidi B1ARK0.
    KOi K04837.
    PhylomeDBi Q9ER60.

    Miscellaneous databases

    NextBioi 364857.
    PROi Q9ER60.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori Q9ER60.

    Family and domain databases

    Gene3Di 1.20.120.350. 4 hits.
    InterProi IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR028826. Na_channel_a4su.
    IPR008052. Na_channel_a4su_mammal.
    IPR001696. Na_channel_asu.
    IPR010526. Na_trans_assoc.
    [Graphical view ]
    PANTHERi PTHR10037:SF193. PTHR10037:SF193. 1 hit.
    Pfami PF00520. Ion_trans. 4 hits.
    PF06512. Na_trans_assoc. 1 hit.
    [Graphical view ]
    PRINTSi PR00170. NACHANNEL.
    PR01665. NACHANNEL4.
    SMARTi SM00015. IQ. 1 hit.
    [Graphical view ]
    PROSITEi PS50096. IQ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse heart Na+ channels: primary structure and function of two isoforms and alternatively spliced variants."
      Zimmer T., Bollensdorff C., Haufe V., Birch-Hirschfeld E., Benndorf K.
      Am. J. Physiol. 282:H1007-H1017(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Heart.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-1841.

    Entry informationi

    Entry nameiSCN4A_MOUSE
    AccessioniPrimary (citable) accession number: Q9ER60
    Secondary accession number(s): A2VDE9, B1ARK0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3