Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calpain-12

Gene

Capn12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.By similarity

Catalytic activityi

Broad endopeptidase specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei105 – 1051By similarity
Active sitei259 – 2591By similarity
Active sitei283 – 2831By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi634 – 64512PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.B32. 3474.

Protein family/group databases

MEROPSiC02.017.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-12 (EC:3.4.22.-)
Alternative name(s):
Calcium-activated neutral proteinase 12
Short name:
CANP 12
Gene namesi
Name:Capn12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1891369. Capn12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720Calpain-12PRO_0000207731Add
BLAST

Proteomic databases

PaxDbiQ9ER56.
PRIDEiQ9ER56.

PTM databases

PhosphoSiteiQ9ER56.

Expressioni

Tissue specificityi

Expression localized to the cortex of the hair follicle during the anagen phase of hair cycle.

Gene expression databases

CleanExiMM_CAPN12.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069055.

Structurei

3D structure databases

ProteinModelPortaliQ9ER56.
SMRiQ9ER56. Positions 27-720.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 341297Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini621 – 65636EF-handPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni342 – 541200Domain IIIAdd
BLAST
Regioni542 – 720179Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ9ER56.
PhylomeDBiQ9ER56.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029545. CAPN12.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF280. PTHR10183:SF280. 2 hits.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ER56-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASGNRKVTI QLVDDGAGTG AGGPQLFKGQ NYEAIRRACL DSGILFRDPC
60 70 80 90 100
FPAGPDALGY DKLGPDSEKA KGVEWKRPHE FCAEPQFICE DMSRTDVCQG
110 120 130 140 150
SLGNCWLLAA AASLTLYPRL LYRVVPPGQG FQDGYAGVFH FQLWQFGRWV
160 170 180 190 200
DVVVDDKLPV REGKLMFVRS EQRNEFWAPL LEKAYAKLHG SYEVMRGGHM
210 220 230 240 250
NEAFVDFTGG VGEVLYLRQN TPGVFAALRH ALAKESLVGA TALSDRGEIR
260 270 280 290 300
TDEGLVKGHA YSVTGTHKMS LGFTKVRLLR LRNPWGRVEW SGPWSDSCPR
310 320 330 340 350
WDMLPSEWRD ALLVKKEDGE FWMELQDFLT HFNTVQICSL SPEVLGPSPA
360 370 380 390 400
GGGWHIHIFQ GRWVRGFNSG GSQPSAENFW TNPQFRLTLL EPDEEEDDDD
410 420 430 440 450
EEGPWGGWGA AGARGPARGG RVPKCTVLLS LIQRNRRCLR AKGLTYLTVG
460 470 480 490 500
FHVFQIPEEL LDLWDSPRSR ALLPGLLRAD RSVFCARRDV SRRCRLPPGH
510 520 530 540 550
YLVVPSASRV GDEADFTLRI FSERSHTAVE IDDVISADLD ALQAPYKPLE
560 570 580 590 600
LELAQLFLEL AGEEEELNAL QLQTLISIAL EPARANTRTP GEIGLRTCEQ
610 620 630 640 650
LVQCFGRGQR LSLHHFQELW GHLMSWQATF DKFDEDASGT MNSCELRLAL
660 670 680 690 700
TAAGFHLNNQ LTQSLTSRYR DSRLRVDFER FVGCAARLTC IFRHCCQHLD
710 720
GGEGVVCLTH KQWSEVATFS
Length:720
Mass (Da):80,588
Last modified:March 1, 2001 - v1
Checksum:i37C07BDF0145B531
GO
Isoform 2 (identifier: Q9ER56-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     341-448: SPEVLGPSPA...LRAKGLTYLT → LPTPGWRRGG...WGMNRDAGRR
     449-720: Missing.

Show »
Length:449
Mass (Da):50,398
Checksum:iF1892CE897094BF3
GO
Isoform 3 (identifier: Q9ER56-3) [UniParc]FASTAAdd to basket

Also known as: variant 3

The sequence of this isoform differs from the canonical sequence as follows:
     460-462: LLD → GDR
     463-720: Missing.

Show »
Length:462
Mass (Da):51,492
Checksum:iE1E5814D4896F5F9
GO
Isoform 4 (identifier: Q9ER56-4) [UniParc]FASTAAdd to basket

Also known as: variant 2

The sequence of this isoform differs from the canonical sequence as follows:
     460-502: LLDLWDSPRS...RCRLPPGHYL → PRALAGTAAR...GGTQRLARRR
     503-720: Missing.

Show »
Length:502
Mass (Da):55,875
Checksum:i816AC59F92226C3C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei341 – 448108SPEVL…LTYLT → LPTPGWRRGGRLPDPQTVVG GGYLLIGLKLREVTLLPDSL SQRWWLCNPGRPHKCWDYEL EPSQTELPPFLLKPLHVSPC LERGTTPTQALGWWALPAPW GMNRDAGRR in isoform 2. 2 PublicationsVSP_007807Add
BLAST
Alternative sequencei449 – 720272Missing in isoform 2. 2 PublicationsVSP_007808Add
BLAST
Alternative sequencei460 – 50243LLDLW…PGHYL → PRALAGTAARRPLGFLRPPR REPSLSPAAWPLPGGTQRLA RRR in isoform 4. 1 PublicationVSP_007811Add
BLAST
Alternative sequencei460 – 4623LLD → GDR in isoform 3. 1 PublicationVSP_007809
Alternative sequencei463 – 720258Missing in isoform 3. 1 PublicationVSP_007810Add
BLAST
Alternative sequencei503 – 720218Missing in isoform 4. 1 PublicationVSP_007812Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ289241 Genomic DNA. Translation: CAC10066.1.
AJ289241 Genomic DNA. Translation: CAC10067.1.
AJ289241 Genomic DNA. Translation: CAC10068.1.
AJ289243 mRNA. Translation: CAC10070.1.
BC028751 mRNA. Translation: AAH28751.1.
CCDSiCCDS52166.1. [Q9ER56-1]
RefSeqiXP_006540316.1. XM_006540253.2. [Q9ER56-4]
UniGeneiMm.476990.

Genome annotation databases

GeneIDi60594.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ289241 Genomic DNA. Translation: CAC10066.1.
AJ289241 Genomic DNA. Translation: CAC10067.1.
AJ289241 Genomic DNA. Translation: CAC10068.1.
AJ289243 mRNA. Translation: CAC10070.1.
BC028751 mRNA. Translation: AAH28751.1.
CCDSiCCDS52166.1. [Q9ER56-1]
RefSeqiXP_006540316.1. XM_006540253.2. [Q9ER56-4]
UniGeneiMm.476990.

3D structure databases

ProteinModelPortaliQ9ER56.
SMRiQ9ER56. Positions 27-720.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069055.

Protein family/group databases

MEROPSiC02.017.

PTM databases

PhosphoSiteiQ9ER56.

Proteomic databases

PaxDbiQ9ER56.
PRIDEiQ9ER56.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi60594.

Organism-specific databases

CTDi147968.
MGIiMGI:1891369. Capn12.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ9ER56.
PhylomeDBiQ9ER56.

Enzyme and pathway databases

BRENDAi3.4.22.B32. 3474.

Miscellaneous databases

ChiTaRSiCapn12. mouse.
PROiQ9ER56.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CAPN12.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029545. CAPN12.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF280. PTHR10183:SF280. 2 hits.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gene structure, chromosomal localization and expression pattern of Capn12, a new member of the calpain large subunit gene family."
    Dear T.N., Meier N.T., Hunn M., Boehm T.
    Genomics 68:152-160(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2; 3 AND 4).
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Mammary gland.

Entry informationi

Entry nameiCAN12_MOUSE
AccessioniPrimary (citable) accession number: Q9ER56
Secondary accession number(s): Q9ER53, Q9ER54, Q9ER55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: March 1, 2001
Last modified: May 11, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.