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Protein

Calpain-12

Gene

Capn12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.By similarity

Catalytic activityi

Broad endopeptidase specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105By similarity1
Active sitei259By similarity1
Active sitei283By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi634 – 645PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.B32. 3474.

Protein family/group databases

MEROPSiC02.017.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-12 (EC:3.4.22.-)
Alternative name(s):
Calcium-activated neutral proteinase 12
Short name:
CANP 12
Gene namesi
Name:Capn12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1891369. Capn12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077311 – 720Calpain-12Add BLAST720

Proteomic databases

PaxDbiQ9ER56.
PRIDEiQ9ER56.

PTM databases

PhosphoSitePlusiQ9ER56.

Expressioni

Tissue specificityi

Expression localized to the cortex of the hair follicle during the anagen phase of hair cycle.

Gene expression databases

CleanExiMM_CAPN12.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069055.

Structurei

3D structure databases

ProteinModelPortaliQ9ER56.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 341Calpain catalyticPROSITE-ProRule annotationAdd BLAST297
Domaini621 – 656EF-handPROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni342 – 541Domain IIIAdd BLAST200
Regioni542 – 720Domain IVAdd BLAST179

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ9ER56.
PhylomeDBiQ9ER56.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029545. CAPN12.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF280. PTHR10183:SF280. 2 hits.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ER56-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASGNRKVTI QLVDDGAGTG AGGPQLFKGQ NYEAIRRACL DSGILFRDPC
60 70 80 90 100
FPAGPDALGY DKLGPDSEKA KGVEWKRPHE FCAEPQFICE DMSRTDVCQG
110 120 130 140 150
SLGNCWLLAA AASLTLYPRL LYRVVPPGQG FQDGYAGVFH FQLWQFGRWV
160 170 180 190 200
DVVVDDKLPV REGKLMFVRS EQRNEFWAPL LEKAYAKLHG SYEVMRGGHM
210 220 230 240 250
NEAFVDFTGG VGEVLYLRQN TPGVFAALRH ALAKESLVGA TALSDRGEIR
260 270 280 290 300
TDEGLVKGHA YSVTGTHKMS LGFTKVRLLR LRNPWGRVEW SGPWSDSCPR
310 320 330 340 350
WDMLPSEWRD ALLVKKEDGE FWMELQDFLT HFNTVQICSL SPEVLGPSPA
360 370 380 390 400
GGGWHIHIFQ GRWVRGFNSG GSQPSAENFW TNPQFRLTLL EPDEEEDDDD
410 420 430 440 450
EEGPWGGWGA AGARGPARGG RVPKCTVLLS LIQRNRRCLR AKGLTYLTVG
460 470 480 490 500
FHVFQIPEEL LDLWDSPRSR ALLPGLLRAD RSVFCARRDV SRRCRLPPGH
510 520 530 540 550
YLVVPSASRV GDEADFTLRI FSERSHTAVE IDDVISADLD ALQAPYKPLE
560 570 580 590 600
LELAQLFLEL AGEEEELNAL QLQTLISIAL EPARANTRTP GEIGLRTCEQ
610 620 630 640 650
LVQCFGRGQR LSLHHFQELW GHLMSWQATF DKFDEDASGT MNSCELRLAL
660 670 680 690 700
TAAGFHLNNQ LTQSLTSRYR DSRLRVDFER FVGCAARLTC IFRHCCQHLD
710 720
GGEGVVCLTH KQWSEVATFS
Length:720
Mass (Da):80,588
Last modified:March 1, 2001 - v1
Checksum:i37C07BDF0145B531
GO
Isoform 2 (identifier: Q9ER56-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     341-448: SPEVLGPSPA...LRAKGLTYLT → LPTPGWRRGG...WGMNRDAGRR
     449-720: Missing.

Show »
Length:449
Mass (Da):50,398
Checksum:iF1892CE897094BF3
GO
Isoform 3 (identifier: Q9ER56-3) [UniParc]FASTAAdd to basket
Also known as: variant 3

The sequence of this isoform differs from the canonical sequence as follows:
     460-462: LLD → GDR
     463-720: Missing.

Show »
Length:462
Mass (Da):51,492
Checksum:iE1E5814D4896F5F9
GO
Isoform 4 (identifier: Q9ER56-4) [UniParc]FASTAAdd to basket
Also known as: variant 2

The sequence of this isoform differs from the canonical sequence as follows:
     460-502: LLDLWDSPRS...RCRLPPGHYL → PRALAGTAAR...GGTQRLARRR
     503-720: Missing.

Show »
Length:502
Mass (Da):55,875
Checksum:i816AC59F92226C3C
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007807341 – 448SPEVL…LTYLT → LPTPGWRRGGRLPDPQTVVG GGYLLIGLKLREVTLLPDSL SQRWWLCNPGRPHKCWDYEL EPSQTELPPFLLKPLHVSPC LERGTTPTQALGWWALPAPW GMNRDAGRR in isoform 2. 2 PublicationsAdd BLAST108
Alternative sequenceiVSP_007808449 – 720Missing in isoform 2. 2 PublicationsAdd BLAST272
Alternative sequenceiVSP_007811460 – 502LLDLW…PGHYL → PRALAGTAARRPLGFLRPPR REPSLSPAAWPLPGGTQRLA RRR in isoform 4. 1 PublicationAdd BLAST43
Alternative sequenceiVSP_007809460 – 462LLD → GDR in isoform 3. 1 Publication3
Alternative sequenceiVSP_007810463 – 720Missing in isoform 3. 1 PublicationAdd BLAST258
Alternative sequenceiVSP_007812503 – 720Missing in isoform 4. 1 PublicationAdd BLAST218

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ289241 Genomic DNA. Translation: CAC10066.1.
AJ289241 Genomic DNA. Translation: CAC10067.1.
AJ289241 Genomic DNA. Translation: CAC10068.1.
AJ289243 mRNA. Translation: CAC10070.1.
BC028751 mRNA. Translation: AAH28751.1.
CCDSiCCDS52166.1. [Q9ER56-1]
RefSeqiXP_006540316.1. XM_006540253.3. [Q9ER56-4]
UniGeneiMm.476990.

Genome annotation databases

GeneIDi60594.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ289241 Genomic DNA. Translation: CAC10066.1.
AJ289241 Genomic DNA. Translation: CAC10067.1.
AJ289241 Genomic DNA. Translation: CAC10068.1.
AJ289243 mRNA. Translation: CAC10070.1.
BC028751 mRNA. Translation: AAH28751.1.
CCDSiCCDS52166.1. [Q9ER56-1]
RefSeqiXP_006540316.1. XM_006540253.3. [Q9ER56-4]
UniGeneiMm.476990.

3D structure databases

ProteinModelPortaliQ9ER56.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069055.

Protein family/group databases

MEROPSiC02.017.

PTM databases

PhosphoSitePlusiQ9ER56.

Proteomic databases

PaxDbiQ9ER56.
PRIDEiQ9ER56.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi60594.

Organism-specific databases

CTDi147968.
MGIiMGI:1891369. Capn12.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiQ9ER56.
PhylomeDBiQ9ER56.

Enzyme and pathway databases

BRENDAi3.4.22.B32. 3474.

Miscellaneous databases

ChiTaRSiCapn12. mouse.
PROiQ9ER56.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CAPN12.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029545. CAPN12.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF280. PTHR10183:SF280. 2 hits.
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN12_MOUSE
AccessioniPrimary (citable) accession number: Q9ER56
Secondary accession number(s): Q9ER53, Q9ER54, Q9ER55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.