Reviewed, UniProtKB/Swiss-Prot Q9ER42 (BARX1_MOUSE)

Names and origin

Protein names

Recommended name:
    Homeobox protein BarH-like 1

Gene names

Name:

Barx1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transcription factor, which is involved in craniofacial development, in odontogenesis and in stomach organogenesis. May have a role in the differentiation of molars from incisors. Plays a role in suppressing endodermal Wnt activity. Binds to a regulatory module of the NCAM promoter. Ref.5 Ref.6
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed predominantly in the facial primordia, developing stomach, and proximal limbs.
Developmental stage	Expressed in areas of the first and second branchial arches, before any apparent cellular or morphologic differentiation. Later in development, all expressing tissue in this region, including the mesenchyme underlying the olfactory epithelium, the primary and secondary palate, the molar tooth papillae, and the stroma of the submandibular gland, appear to be derived from ectomesenchyme of neural crest origin. By day 16.5, all areas except the developing molars are BARX1-negative. In addition, BARX1 marks the area of the future stomach in the primitive gut at embryonic day 9.5, and is present in the mesenchymal wall of the stomach until embryonic day 16.5. Ref.6 Ref.4
Sequence similarities	Belongs to the BAR homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Gene Ontology (GO)
Biological process	anterior/posterior pattern formation Ref.6 Inferred from mutant phenotype. Source: MGI cell-cell signaling Ref.5 Inferred from genetic interaction. Source: MGI digestive system development Inferred from mutant phenotype. Source: MGI epithelial cell differentiation Ref.5 Inferred from mutant phenotype. Source: MGI negative regulation of Wnt receptor signaling pathway Ref.5 Ref.6 Inferred from mutant phenotype. Source: MGI regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro spleen development Ref.6 Inferred from mutant phenotype. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	Homeobox protein BarH-like 1		PRO_0000048835
Regions
	DNA binding	142 – 201	60	Homeobox
	Compositional bias	44 – 53	10	Poly-Ala

Sequences

	Sequence		Length	Mass (Da)
	Q9ER42-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 48586B28F4A23FCC	FASTA	254	27,282
	10 20 30 40 50 60 MQRPGEPGAA RFGPPEGCAD HRPHRYRSFM IEEILTEPPG PKGAAPAAAA AAAGELLKFG 70 80 90 100 110 120 VQALLAARPF HSHLAVLKAE QAAVFKFPLA PLGCSGLGSA LLAAGPGMPG PAGASHLPLE 130 140 150 160 170 180 LQLRGKLEAA GSGEPGAKAK KGRRSRTVFT ELQLMGLEKR FEKQKYLSTP DRIDLAESLG 190 200 210 220 230 240 LSQLQVKTWY QNRRMKWKKI VLQGGGLESP TKPKGRPKKN SIPTSEQLTE QERAKETEKP 250 AETPGEPSDR NCED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Buchner G. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Head.
[2]	"Characterization of the mouse Barx1 gene." Meech R., Edelman D.B., Jones F.S. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-254. Strain: BALB/c.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 81-254. Tissue: Brain.
[4]	"Barx1, a new mouse homeodomain transcription factor expressed in cranio-facial ectomesenchyme and the stomach." Tissier-Seta J.-P., Mucchielli M.L., Mark M., Mattei M.-G., Goridis C., Brunet J.-F. Mech. Dev. 51:3-15(1995) [PubMed: 7669690] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 108-254, DEVELOPMENTAL STAGE. Strain: NMRI.
[5]	"The stomach mesenchymal transcription factor Barx1 specifies gastric epithelial identity through inhibition of transient Wnt signaling." Kim B.-M., Buchner G., Miletich I., Sharpe P.T., Shivdasani R.A. Dev. Cell 8:611-622(2005) [PubMed: 15809042] [Abstract] Cited for: FUNCTION.
[6]	"Independent functions and mechanisms for homeobox gene Barx1 in patterning mouse stomach and spleen." Kim B.M., Miletich I., Mao J., McMahon A.P., Sharpe P.A., Shivdasani R.A. Development 134:3603-3613(2007) [PubMed: 17855428] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ297677 mRNA. Translation: CAC10357.1. AF277160 Genomic DNA. Translation: AAG18573.1. BC119047 mRNA. Translation: AAI19048.2. BC119049 mRNA. Translation: AAI19050.2. Y07960 mRNA. Translation: CAA69257.1.
IPI	IPI00111554.
RefSeq	NP_031552.2.
UniGene	Mm.42241
3D structure databases
SMR	Q9ER42. Positions 134-199.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ER42.
PTM databases
PhosphoSite	Q9ER42.
Proteomic databases
PRIDE	Q9ER42.
Genome annotation databases
Ensembl	ENSMUST00000021813; ENSMUSP00000021813; ENSMUSG00000021381; Mus musculus. [Genome view]
GeneID	12022.
KEGG	mmu:12022.
UCSC	uc007qil.1. mouse.
Organism-specific databases
CTD	12022.
MGI	MGI:103124. Barx1.
Phylogenomic databases
eggNOG	roNOG16083.
HOGENOM	HBG717712.
HOVERGEN	Q9ER42.
InParanoid	Q9ER42.
OMA	LLKFGVQ.
OrthoDB	EOG9QC3QS.
PhylomeDB	Q9ER42.
Gene expression databases
ArrayExpress	Q9ER42.
Bgee	Q9ER42.
CleanEx	MM_BARX1.
Genevestigator	Q9ER42.
GermOnline	ENSMUSG00000021381. Mus musculus.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR020479. Homeobox_region. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR000047. HTH_lambrepressr. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX. PR00031. HTHREPRESSR.
SMART	SM00389. HOX. 1 hit. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	280251.
SOURCE	Search...

Entry information

Entry name

BARX1_MOUSE

Accession

Primary (citable) accession number: Q9ER42
Secondary accession number(s): O09066 , P70159, Q0VF04, Q9ERV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)