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Protein

Regulating synaptic membrane exocytosis protein 2

Gene

Rims2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rab effector involved in exocytosis. May act as scaffold protein. Plays a role in dendrite formation by melanocytes (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri126 – 182FYVE-typePROSITE-ProRule annotationAdd BLAST57

GO - Molecular functioni

  • ion channel binding Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • calcium ion regulated exocytosis Source: MGI
  • calcium ion-regulated exocytosis of neurotransmitter Source: MGI
  • cAMP-mediated signaling Source: MGI
  • insulin secretion Source: MGI
  • intracellular protein transport Source: InterPro
  • neurotransmitter secretion Source: MGI
  • positive regulation of dendrite extension Source: MGI
  • positive regulation of excitatory postsynaptic potential Source: ParkinsonsUK-UCL
  • positive regulation of gene expression Source: ParkinsonsUK-UCL
  • positive regulation of inhibitory postsynaptic potential Source: ParkinsonsUK-UCL
  • regulation of exocytosis Source: MGI
  • regulation of membrane potential Source: MGI
  • regulation of synaptic vesicle exocytosis Source: GO_Central
  • spontaneous neurotransmitter secretion Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Biological processi

Differentiation, Exocytosis, Neurotransmitter transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Regulating synaptic membrane exocytosis protein 2
Alternative name(s):
Rab-3-interacting molecule 2
Short name:
RIM 2
Rab-3-interacting protein 2
Gene namesi
Name:Rims2
Synonyms:Rab3ip2, Rim2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2152972. Rims2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902021 – 1530Regulating synaptic membrane exocytosis protein 2Add BLAST1530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei409PhosphoserineCombined sources1
Modified residuei698PhosphothreonineBy similarity1
Modified residuei800PhosphoserineCombined sources1
Modified residuei803PhosphoserineCombined sources1
Modified residuei1095PhosphoserineBy similarity1
Modified residuei1175PhosphoserineBy similarity1
Modified residuei1251PhosphoserineBy similarity1
Modified residuei1515PhosphoserineCombined sources1
Modified residuei1518PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9EQZ7.

PTM databases

iPTMnetiQ9EQZ7.
PhosphoSitePlusiQ9EQZ7.

Expressioni

Tissue specificityi

Detected in testis, pituitary and an insulinoma cell line. Detected at low levels in cerebellar cortex.1 Publication

Gene expression databases

BgeeiENSMUSG00000037386.
CleanExiMM_RIMS2.
ExpressionAtlasiQ9EQZ7. baseline and differential.
GenevisibleiQ9EQZ7. MM.

Interactioni

Subunit structurei

Interacts with TSPOAP1 and RIMBP2. Interacts with PPFIA3 and PPFIA4. Interacts via its zinc finger with the first C2 domain of UNC13A. Forms a complex consisting of UNC13A, RIMS2 and RAB3A (By similarity). Heterodimer with PCLO. Part of a ternary complex involving PCLO and EPAC2. Interacts with RAB3A and RAB3B that have been activated by GTP-binding. Interacts with RAB3C, RAB3D and RAB26.By similarity3 Publications

GO - Molecular functioni

  • ion channel binding Source: MGI

Protein-protein interaction databases

BioGridi228026. 8 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ9EQZ7.
SMRiQ9EQZ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 194RabBDPROSITE-ProRule annotationAdd BLAST169
Domaini677 – 763PDZPROSITE-ProRule annotationAdd BLAST87
Domaini816 – 922C2 1PROSITE-ProRule annotationAdd BLAST107
Domaini1376 – 1478C2 2PROSITE-ProRule annotationAdd BLAST103

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1169 – 1202Ser-richAdd BLAST34

Sequence similaritiesi

Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 RabBD (Rab-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri126 – 182FYVE-typePROSITE-ProRule annotationAdd BLAST57

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00550000074588.
HOGENOMiHOG000082403.
HOVERGENiHBG058147.
InParanoidiQ9EQZ7.
KOiK15297.
PhylomeDBiQ9EQZ7.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
2.60.40.150. 2 hits.
3.30.40.10. 2 hits.
InterProiIPR000008. C2_dom.
IPR001478. PDZ.
IPR010911. Rab_BD.
IPR030631. RIM2.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR12157:SF15. PTHR12157:SF15. 2 hits.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF50156. SSF50156. 1 hit.
SSF57903. SSF57903. 2 hits.
PROSITEiPS50004. C2. 2 hits.
PS50106. PDZ. 1 hit.
PS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9EQZ7-1) [UniParc]FASTAAdd to basket
Also known as: RIM2-alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAPLGPRGR PAPTPAASQP PPQPEMPDLS HLTEEERKII LAVMDRQKKE
60 70 80 90 100
EEKEQSVLKI KEEHKAQPTQ WFPFSGITEL VNNVLQPQQK QPNEKEPQTK
110 120 130 140 150
LHQQFEMYKE QVKKMGEESQ QQQEQKGDAP TCGICHKTKF ADGCGHNCSY
160 170 180 190 200
CQTKFCARCG GRVSLRSNKV MWVCNLCRKQ QEILTKSGAW FYNSGSNTLQ
210 220 230 240 250
QPDQKVPRGL RNEEAPQEKK AKLHEQPQFQ GAPGDLSVPA VEKGRAHGLT
260 270 280 290 300
RQDTIKNGSG VKHQIASDMP SDRKRSPSVS RDQNRRYEQS EEREDYSQYV
310 320 330 340 350
PSDGTMPRSP SDYADRRSQR EPQFYEEPGH LNYRDSNRRG HRHSKEYIVD
360 370 380 390 400
DEDVESRDEY ERQRREEEYQ ARYRSDPNLA RYPVKPQPYE EQMRIHAEVS
410 420 430 440 450
RARHERRHSD VSLANAELED SRISLLRMDR PSRQRSVSER RAAMENQRSY
460 470 480 490 500
SMERTREAQG QSSYPQRTSN HSPPTPRRSP IPLDRPDMRR ADSLRKQHHL
510 520 530 540 550
DPSSAVRKTK REKMETMLRN DSLSSDQSES VRPPPPRPHK SKKGGKMRQV
560 570 580 590 600
SLSSSEEELA STPEYTSCDD VELESESVSE KGDSQKGKRK TSEQGVLSDS
610 620 630 640 650
NTRSERQKKR MYYGGHSLEE DLEWSEPQIK DSGVDTCSST TLNEEHSHSD
660 670 680 690 700
KHPVTWQPSK DGDRLIGRIL LNKRLKDGSV PRDSGAMLGL KVVGGKMTES
710 720 730 740 750
GRLCAFITKV KKGSLADTVG HLRPGDEVLE WNGRLLQGAT FEEVYNIILE
760 770 780 790 800
SKPEPQVELV VSRPIGDIPR IPDSTHAQLE SSSSSFESQK MDRPSISVTS
810 820 830 840 850
PMSPGMLRDV PQFLSGQLSI KLWFDKVGHQ LIVTILGAKD LPSREDGRPR
860 870 880 890 900
NPYVKIYFLP DRSDKNKRRT KTVKKTLEPK WNQTFIYSPV HRREFRERML
910 920 930 940 950
EITLWDQARV REEESEFLGE ILIELETALL DDEPHWYKLQ THDVSSLPLP
960 970 980 990 1000
RPSPYLPRRQ LHGESPTRRL QRSKRISDSE VSDYDCEDGV GVVSDYRHNG
1010 1020 1030 1040 1050
RDLQSSTLSV PEQVMSSNHC SPSGSPHRVD VIGRTRSWSP SAPPPQRNVE
1060 1070 1080 1090 1100
QGHRGTRATG HYNTISRMDR HRVMDDHYSS DRDRSHPRTG SVQTSPSSTP
1110 1120 1130 1140 1150
GTGRRGRQLP QLPPKGTLER SAMDIEERNR QMKLNKYKQV AGSDPRLEQD
1160 1170 1180 1190 1200
YHSKYRSGWD PHRGADTVST KSSDSDVSDV SAVSRTSSAS RFSSTSYMSV
1210 1220 1230 1240 1250
QSERPRGNRK ISVFTSKMQN RQMGVSGKNL TKSTSISGDM CSLEKNDGSQ
1260 1270 1280 1290 1300
SDTAVGALGT SGKKRRSSIG AKMVAIVGLS RKSRSASQLS QTEGGGKKLR
1310 1320 1330 1340 1350
STVQRSTETG LAVEMRNWMT RQASRESTDG SMNSYSSEGN LIFPGVRLAS
1360 1370 1380 1390 1400
DSQFSDFLDG LGPAQLVGRQ TLATPAMGDI QVGMMDKKGQ LEVEIIRARG
1410 1420 1430 1440 1450
LVVKPGSKTL PAPYVKVYLL DNGVCIAKKK TKVARKTLEP LYQQLLSFEE
1460 1470 1480 1490 1500
SPQGRVLQII VWGDYGRMDH KSFMGVAQIL LDELELSNMV IGWFKLFPPS
1510 1520 1530
SLVDPTLAPL TRRASQSSLE SSTGPSYSRS
Length:1,530
Mass (Da):172,863
Last modified:March 1, 2001 - v1
Checksum:i58CF11BF7152357D
GO
Isoform 2 (identifier: Q9EQZ7-2) [UniParc]FASTAAdd to basket
Also known as: RIM2-gamma

The sequence of this isoform differs from the canonical sequence as follows:
     1-1245: Missing.
     1246-1292: NDGSQSDTAV...SRSASQLSQT → MGRQGLGGTG...CMNSLEEDEG

Note: No experimental confirmation available.
Show »
Length:285
Mass (Da):30,972
Checksum:iFC17F384B1F97E4E
GO
Isoform 3 (identifier: Q9EQZ7-3) [UniParc]FASTAAdd to basket
Also known as: RIM2-beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-232: Missing.
     233-272: PGDLSVPAVE...HQIASDMPSD → MQFETLRQVC...ELFGQTLNNA

Note: No experimental confirmation available.
Show »
Length:1,298
Mass (Da):146,793
Checksum:iC3CC6365227441FC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0081811 – 1245Missing in isoform 2. 1 PublicationAdd BLAST1245
Alternative sequenceiVSP_0081821 – 232Missing in isoform 3. 1 PublicationAdd BLAST232
Alternative sequenceiVSP_008183233 – 272PGDLS…DMPSD → MQFETLRQVCNSVLSHFHGV FSSPPNILQNELFGQTLNNA in isoform 3. 1 PublicationAdd BLAST40
Alternative sequenceiVSP_0081841246 – 1292NDGSQ…QLSQT → MGRQGLGGTGAAGRSMQRSQ SRSSLSASFEALAGYFPCMN SLEEDEG in isoform 2. 1 PublicationAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021131 mRNA. Translation: BAB18975.1.
AK032619 mRNA. Translation: BAC27953.1.
AK083172 mRNA. Translation: BAC38794.1.
CCDSiCCDS37067.1. [Q9EQZ7-1]
RefSeqiNP_001243313.1. NM_001256384.1. [Q9EQZ7-2]
NP_444501.1. NM_053271.2. [Q9EQZ7-1]
UniGeneiMm.309296.

Genome annotation databases

EnsembliENSMUST00000082054; ENSMUSP00000080711; ENSMUSG00000037386. [Q9EQZ7-1]
GeneIDi116838.
KEGGimmu:116838.
UCSCiuc007vod.2. mouse. [Q9EQZ7-1]
uc007voh.2. mouse. [Q9EQZ7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021131 mRNA. Translation: BAB18975.1.
AK032619 mRNA. Translation: BAC27953.1.
AK083172 mRNA. Translation: BAC38794.1.
CCDSiCCDS37067.1. [Q9EQZ7-1]
RefSeqiNP_001243313.1. NM_001256384.1. [Q9EQZ7-2]
NP_444501.1. NM_053271.2. [Q9EQZ7-1]
UniGeneiMm.309296.

3D structure databases

ProteinModelPortaliQ9EQZ7.
SMRiQ9EQZ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228026. 8 interactors.

PTM databases

iPTMnetiQ9EQZ7.
PhosphoSitePlusiQ9EQZ7.

Proteomic databases

PRIDEiQ9EQZ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000082054; ENSMUSP00000080711; ENSMUSG00000037386. [Q9EQZ7-1]
GeneIDi116838.
KEGGimmu:116838.
UCSCiuc007vod.2. mouse. [Q9EQZ7-1]
uc007voh.2. mouse. [Q9EQZ7-2]

Organism-specific databases

CTDi9699.
MGIiMGI:2152972. Rims2.

Phylogenomic databases

GeneTreeiENSGT00550000074588.
HOGENOMiHOG000082403.
HOVERGENiHBG058147.
InParanoidiQ9EQZ7.
KOiK15297.
PhylomeDBiQ9EQZ7.

Miscellaneous databases

PROiQ9EQZ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037386.
CleanExiMM_RIMS2.
ExpressionAtlasiQ9EQZ7. baseline and differential.
GenevisibleiQ9EQZ7. MM.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
2.60.40.150. 2 hits.
3.30.40.10. 2 hits.
InterProiIPR000008. C2_dom.
IPR001478. PDZ.
IPR010911. Rab_BD.
IPR030631. RIM2.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR12157:SF15. PTHR12157:SF15. 2 hits.
PfamiPF00168. C2. 2 hits.
PF02318. FYVE_2. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF50156. SSF50156. 1 hit.
SSF57903. SSF57903. 2 hits.
PROSITEiPS50004. C2. 2 hits.
PS50106. PDZ. 1 hit.
PS50916. RABBD. 1 hit.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIMS2_MOUSE
AccessioniPrimary (citable) accession number: Q9EQZ7
Secondary accession number(s): Q8C433, Q8CCK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.