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Protein

Prostaglandin reductase 1

Gene

Ptgr1

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4.1 Publication

Catalytic activityi

A n-alkanal + NAD(P)+ = an alk-2-enal + NAD(P)H.
11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.

Kineticsi

  1. KM=93 µM for LTB4
  2. KM=35 µM for 15-keto-PGE2
  1. Vmax=1.7 nmol/min/mg enzyme with LTB4 as substrate
  2. Vmax=345 nmol/min/mg enzyme with 15-keto-PGE2 as substrate

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei178NADP1 Publication1
Binding sitei193NADP1 Publication1
Binding sitei217NADP1 Publication1
Binding sitei321NADP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi152 – 155NADP1 Publication4
Nucleotide bindingi239 – 245NADP1 Publication7
Nucleotide bindingi270 – 272NADP1 Publication3

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin reductase 1 (EC:1.3.1.-)
Short name:
PRG-1
Alternative name(s):
15-oxoprostaglandin 13-reductase (EC:1.3.1.48)
Short name:
PGR
NADP-dependent leukotriene B4 12-hydroxydehydrogenase (EC:1.3.1.74)
Short name:
12-HD
Short name:
LTB4
Gene namesi
Name:Ptgr1
Synonyms:Ltb4dh
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002180641 – 329Prostaglandin reductase 1Add BLAST329

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphothreonineBy similarity1
Modified residuei20PhosphoserineBy similarity1
Modified residuei178N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ9EQZ5.

Expressioni

Tissue specificityi

Detected in small intestine, kidney, liver, spleen and stomach (at protein level). Detected in small intestine, kidney and liver.1 Publication

Interactioni

Subunit structurei

Monomer or homodimer.1 Publication

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Beta strandi14 – 16Combined sources3
Helixi19 – 21Combined sources3
Beta strandi22 – 28Combined sources7
Beta strandi37 – 45Combined sources9
Helixi49 – 53Combined sources5
Turni54 – 56Combined sources3
Beta strandi67 – 75Combined sources9
Beta strandi84 – 87Combined sources4
Beta strandi91 – 99Combined sources9
Beta strandi102 – 104Combined sources3
Helixi115 – 119Combined sources5
Turni120 – 122Combined sources3
Helixi124 – 134Combined sources11
Turni135 – 137Combined sources3
Beta strandi144 – 149Combined sources6
Helixi153 – 164Combined sources12
Beta strandi168 – 175Combined sources8
Helixi176 – 184Combined sources9
Beta strandi188 – 192Combined sources5
Turni193 – 195Combined sources3
Helixi199 – 206Combined sources8
Beta strandi211 – 218Combined sources8
Helixi220 – 227Combined sources8
Beta strandi230 – 238Combined sources9
Turni244 – 246Combined sources3
Helixi257 – 262Combined sources6
Beta strandi266 – 269Combined sources4
Helixi272 – 274Combined sources3
Helixi278 – 292Combined sources15
Beta strandi300 – 304Combined sources5
Helixi306 – 308Combined sources3
Helixi309 – 317Combined sources9
Beta strandi322 – 328Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V3TX-ray2.30A/B1-329[»]
1V3UX-ray2.00A/B1-329[»]
1V3VX-ray2.00A/B1-329[»]
2DM6X-ray2.00A/B1-329[»]
ProteinModelPortaliQ9EQZ5.
SMRiQ9EQZ5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9EQZ5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG055024.
InParanoidiQ9EQZ5.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR014190. B4_12hDH.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02825. B4_12hDH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9EQZ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKAKSWTLK KHFQGKPTQS DFELKTVELP PLKNGEVLLE ALFLSVDPYM
60 70 80 90 100
RIASKRLKEG AVMMGQQVAR VVESKNSAFP AGSIVLAQSG WTTHFISDGK
110 120 130 140 150
GLEKLLTEWP DKLPLSLALG TIGMPGLTAY FGLLEVCGVK GGETVLVSAA
160 170 180 190 200
AGAVGSVVGQ IAKLKGCKVV GAAGSDEKIA YLKQIGFDAA FNYKTVNSLE
210 220 230 240 250
EALKKASPDG YDCYFDNVGG EFLNTVLSQM KDFGKIAICG AISVYNRMDQ
260 270 280 290 300
LPPGPSPESI IYKQLRIEGF IVYRWQGDVR EKALRDLMKW VLEGKIQYHE
310 320
HVTKGFENMP AAFIEMLNGA NLGKAVVTA
Length:329
Mass (Da):35,730
Last modified:March 1, 2001 - v1
Checksum:i8C5D86D9D2C9FEA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021219 mRNA. Translation: BAB20289.1.
RefSeqiNP_001166451.1. NM_001172980.1.

Genome annotation databases

GeneIDi100135572.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021219 mRNA. Translation: BAB20289.1.
RefSeqiNP_001166451.1. NM_001172980.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V3TX-ray2.30A/B1-329[»]
1V3UX-ray2.00A/B1-329[»]
1V3VX-ray2.00A/B1-329[»]
2DM6X-ray2.00A/B1-329[»]
ProteinModelPortaliQ9EQZ5.
SMRiQ9EQZ5.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9EQZ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100135572.

Organism-specific databases

CTDi22949.

Phylogenomic databases

HOVERGENiHBG055024.
InParanoidiQ9EQZ5.

Miscellaneous databases

EvolutionaryTraceiQ9EQZ5.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR014190. B4_12hDH.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02825. B4_12hDH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPTGR1_CAVPO
AccessioniPrimary (citable) accession number: Q9EQZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.