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Q9EQW7 (KI13A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kinesin-like protein KIF13A
Gene names
Name:Kif13a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length1749 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plus end-directed microtubule-dependent motor protein involved in intracellular transport and regulating various processes such as mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis and cytokinesis. During melanosome maturation, required for delivering melanogenic enzymes from recycling endosomes to nascent melanosomes by creating peripheral recycling endosomal subdomains in melanocytes. Also required for the abcission step in cytokinesis: mediates translocation of ZFYVE26, and possibly TTC19, to the midbody during cytokinesis By similarity. Mediates the transport of M6PR-containing vesicles from trans-Golgi network to the plasma membrane via direct interaction with the AP-1 complex. Ref.1

Subunit structure

Interacts with AP1G1 and AP1G2. Interacts with ZFYVE26 By similarity. Interacts with AP2B1. Ref.1

Subcellular location

Golgi apparatus membrane. Cytoplasm › cytoskeleton › centrosome Probable. Midbody By similarity. Endosome membrane By similarity. Note: Recruited to the midbody during cytokinesis By similarity. Ref.1

Developmental stage

At E9.5, expressed essentially in the central nervous system. Widely expressed at E10.5. At E11.5, expressed in the telencephaon, otic vesicles, limb buds, nasal processes and urogenital bud. Ref.3

Sequence similarities

Belongs to the kinesin-like protein family.

Contains 1 FHA domain.

Contains 1 kinesin-motor domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Protein transport
Transport
   Cellular componentCytoplasm
Cytoskeleton
Endosome
Golgi apparatus
Membrane
Microtubule
   DomainCoiled coil
   LigandATP-binding
Nucleotide-binding
   Molecular functionMotor protein
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGolgi to plasma membrane protein transport

Inferred from direct assay Ref.1. Source: UniProtKB

cargo loading into vesicle

Inferred from sequence or structural similarity. Source: UniProtKB

cell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cytokinesis

Inferred from sequence or structural similarity. Source: UniProtKB

endosome to lysosome transport

Inferred from sequence or structural similarity. Source: UniProtKB

melanosome organization

Inferred from sequence or structural similarity. Source: UniProtKB

plus-end-directed vesicle transport along microtubule

Inferred from direct assay Ref.1. Source: UniProtKB

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

centrosome

Inferred from sequence or structural similarity. Source: UniProtKB

endosome membrane

Inferred from sequence or structural similarity. Source: UniProtKB

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule associated complex

Inferred from electronic annotation. Source: InterPro

midbody

Inferred from sequence or structural similarity. Source: UniProtKB

trans-Golgi network membrane

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule motor activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17491749Kinesin-like protein KIF13A
PRO_0000125447

Regions

Domain1 – 277277Kinesin-motor
Domain469 – 51951FHA
Nucleotide binding102 – 1098ATP By similarity
Coiled coil359 – 43173 Potential
Coiled coil552 – 775224 Potential
Coiled coil1086 – 112641 Potential
Coiled coil1475 – 149925 Potential

Amino acid modifications

Modified residue12741Phosphoserine By similarity
Modified residue14811Phosphoserine Ref.4
Modified residue16001Phosphoserine Ref.5
Modified residue16501Phosphoserine Ref.4

Experimental info

Sequence conflict1001A → T in BAA22389. Ref.2
Sequence conflict1571V → A in BAA22389. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9EQW7 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: DAE9E026127DDBF2

FASTA1,749195,813
        10         20         30         40         50         60 
MSDTKVKVAV RVRPMNRREL ELNTKCVVEM EGNQTVLHPP PSNTKQGERK PPKVFAFDYC 

        70         80         90        100        110        120 
FWSMDESNTT KYAGQEVVFK CLGEGILEKA FQGYNACIFA YGQTGSGKSF SMMGHAEQLG 

       130        140        150        160        170        180 
LIPRLCCALF QRIALEQNES QTFKVEVSYM EIYNEKVRDL LDPKGSRQSL KVREHKVLGP 

       190        200        210        220        230        240 
YVDGLSQLAV TSFEDIESLM SEGNKSRTVA ATNMNEESSR SHAVFNIIIT QTLYDLQSGN 

       250        260        270        280        290        300 
SGEKVSKVSL VDLAGSERVS KTGAAGERLK EGSNINKSLT TLGLVISSLA DQAAGKGKNK 

       310        320        330        340        350        360 
FVPYRDSVLT WLLKDNLGGN SQTSMIATIS PAADNYEETL STLRYADRAK RIVNHAVVNE 

       370        380        390        400        410        420 
DPNAKVIREL REEVEKLREQ LSKAEAMKPP ELKEKLEESE KLIKELTVTW EEKLRKTEAI 

       430        440        450        460        470        480 
AQERQRQLES MGISLETSGI KVGDDKCYLV NLNADPALNE LLVYYLKDHT RVGADTSQDI 

       490        500        510        520        530        540 
QLFGIGIQPE HCEIDIAADG DITLTPKENA RSCVNGTLVC NTTQLWHGDR ILWGNNHFFR 

       550        560        570        580        590        600 
INLPKRKRRD WLKDFERETS SAEHDLDAAS EASSEPDYNY EFAQMEVIMK TLNSNDPVQN 

       610        620        630        640        650        660 
VVQVLEKQYL EEKRTALEEQ RLMYERELEQ LRQQLSPERQ PPSSASDRLA YSSQTAQQKV 

       670        680        690        700        710        720 
TQWAEERDEL FRQSLAKLRE QLVKANTLVR EANFLAEEMS KLTDYQVTLQ IPAANLSANR 

       730        740        750        760        770        780 
KRGAIVSEPA IQARRKGKGT QVWTIEKLEN KLIDMRDLYQ EWKENVPEAK RLYGKRGDPF 

       790        800        810        820        830        840 
YEAQENHNLI GVANVFLECL FCDVKLQYAV PIISQQGEVA GRLHVEVTRI TGTIPERMAE 

       850        860        870        880        890        900 
DDSSENSSES GSLEVVDSSG EVIHRVKKLT CRVIIKEATG LPISLSNFVF CQYTFWDQCE 

       910        920        930        940        950        960 
STVAAPVVDP DVPSPQSKDA QYTVTFSHCK DYVVTVTEEF LEFISDGALA IEVWGHRCAG 

       970        980        990       1000       1010       1020 
NGSPIWEVDS LHAKTRTLHD RWNEVTRRIE VWISILELNE LGDYAAVELH QAKDVNTGGV 

      1030       1040       1050       1060       1070       1080 
FQLRQGHSRR VQVTVKPVQH SGTLPLMVEA ILSVSIGCVT ARSTKLQRGL DSYQEEDLNC 

      1090       1100       1110       1120       1130       1140 
VRERWSDALI KRREYLDEQI KKVSNKKEKT EDDMEREARL VEQWVGLTEE RNAVLVPAPG 

      1150       1160       1170       1180       1190       1200 
SGIPGAPADW VPPPGMETHI PVLFLDLNAD DLSANEQLVG PHASGVNSIL PKEHGSQFFY 

      1210       1220       1230       1240       1250       1260 
LPIIKHSDDE VSATASWDSS VHDSLHLNRV TPQNERIYLI VKTTVQLSHP AAMELVLRKR 

      1270       1280       1290       1300       1310       1320 
IAANIYNKQS FTQSLKRRIS LINICYSCGV TYEIVSNIPK ATEEIEDRET LALLAARSEN 

      1330       1340       1350       1360       1370       1380 
EGTLDGETYI EKYTRGVLQV ENILSLERLR QAVTVKEALS TKARHIRRSL STPNVHNVSS 

      1390       1400       1410       1420       1430       1440 
SRPDLSGFDE DDKGWPENQL DVSDYSSSYQ DVACYGTLPR DSPRRSKEGC PSENPHALTV 

      1450       1460       1470       1480       1490       1500 
SPFKAFSPQP PKFFKPLMPV KEEHKKRLAL EARPLLSQED SEEEENELEA LSRKLMLTQP 

      1510       1520       1530       1540       1550       1560 
YVPVEFADFS VYNASLENRE WSSSKADLTD SRALEKAVSR SPTTSSLTSG YFSHSASNAT 

      1570       1580       1590       1600       1610       1620 
LSDMAVPSSD SSDQLAVSTK EVECAEPPGP SLAPDVRRAS NQELTEVGRG SGKDETIAVP 

      1630       1640       1650       1660       1670       1680 
LEENSALPKG TPSPQSIPEE SSRMPCRTAS CSELDVGPSK DGHQAREFCP GEVTIEHTTN 

      1690       1700       1710       1720       1730       1740 
ILEDHSFTEF MGVSDGKDFD GLADCSVGEP SRRRALTNET DHKGIPERPP DADRLHPKIE 


NDQEATATR

« Hide

References

« Hide 'large scale' references
[1]"A novel motor, KIF13A, transports mannose-6-phosphate receptor to plasma membrane through direct interaction with AP-1 complex."
Nakagawa T., Setou M., Seog D.H., Ogasawara K., Dohmae N., Takio K., Hirokawa N.
Cell 103:569-581(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH AP2B1.
Strain: ICR.
Tissue: Brain.
[2]"Identification and classification of 16 new kinesin superfamily (KIF) proteins in mouse genome."
Nakagawa T., Tanaka Y., Matsuoka E., Kondo S., Okada Y., Noda Y., Kanai Y., Hirokawa N.
Proc. Natl. Acad. Sci. U.S.A. 94:9654-9659(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 98-257.
Strain: ICR.
[3]"Identification of the human KIF13A gene homologous to Drosophila kinesin-73 and candidate for schizophrenia."
Jamain S., Quach H., Fellous M., Bourgeron T.
Genomics 74:36-44(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1481 AND SER-1650, MASS SPECTROMETRY.
Tissue: Liver.
[5]"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry."
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M.
J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1600, MASS SPECTROMETRY.
Tissue: Melanoma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB037923 mRNA. Translation: BAB16346.1.
AB001429 mRNA. Translation: BAA22389.1.
IPIIPI00111639.
UniGeneMm.342703.

3D structure databases

ProteinModelPortalQ9EQW7.
SMRQ9EQW7. Positions 2-380, 391-550.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000055304.

PTM databases

PhosphoSiteQ9EQW7.

Proteomic databases

PaxDbQ9EQW7.
PRIDEQ9EQW7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCuc007qhn.1. mouse.

Organism-specific databases

MGIMGI:1098264. Kif13a.

Phylogenomic databases

eggNOGCOG5059.
HOGENOMHOG000230729.
HOVERGENHBG052244.
InParanoidQ9EQW7.
OrthoDBEOG4JM7NT.

Gene expression databases

CleanExMM_KIF13A.
GenevestigatorQ9EQW7.
GermOnlineENSMUSG00000021375. Mus musculus.

Family and domain databases

Gene3D2.60.200.20. 1 hit.
3.40.850.10. 1 hit.
InterProIPR000253. FHA_dom.
IPR022140. KIF1B.
IPR022164. Kinesin-like.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamPF12473. DUF3694. 2 hits.
PF00498. FHA. 1 hit.
PF12423. KIF1B. 1 hit.
PF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSPR00380. KINESINHEAVY.
SMARTSM00240. FHA. 1 hit.
SM00129. KISc. 1 hit.
[Graphical view]
SUPFAMSSF49879. SMAD_FHA. 1 hit.
PROSITEPS50006. FHA_DOMAIN. False negative.
PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit.
PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSKIF13A. mouse.
SOURCESearch...

Entry information

Entry nameKI13A_MOUSE
AccessionPrimary (citable) accession number: Q9EQW7
Secondary accession number(s): O35062
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: March 1, 2001
Last modified: May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents