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Q9EQW6 (OLIG2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Oligodendrocyte transcription factor 2

Short name=Oligo2
Gene names
Name:Olig2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. Ref.5 Ref.6

Subunit structure

Interacts with NKX2-2. Ref.9

Subcellular location

Nucleus. Cytoplasm. Note: The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2. Ref.7 Ref.9

Tissue specificity

Expressed specifically in the brain. Ref.1

Developmental stage

Expressed in the ventral spinal cord as early as 9.5 dpc. Expression becomes progressively restricted to a narrow zone within the ventral neuroepithelium of the spinal cord. In the 14.5 dpc spinal cord, expressed in the oligodendrocyte progenitors of the ventral ventricular zone, but not dorsal root ganglia Schwann cells. Also expressed scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. In the brain, from 10.5 through 14.5 dpc, expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. By 15.5 dpc, dispersed throughout the gray matter, with little or no residual expression in the ventricular zone. In the postnatal brain, present preferentially in the white matter, such as corpus callosum and cerebellar medulla. Expressed in the 13.5 and 14.5 dpc retina and in the olfactory epithelium from 11.5 dpc onward. Ref.1 Ref.2

Induction

By SHH. Also induced by NKX6-1 in the developing spinal cord, but not in the rostral hindbrain. Ref.4 Ref.8

Domain

The bHLH is essential for interaction with NKX2-2.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmyelination

Inferred from mutant phenotype PubMed 16436615. Source: MGI

negative regulation of neuron differentiation

Inferred from direct assay PubMed 11566099. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15655114. Source: MGI

nervous system development

Inferred from direct assay Ref.9. Source: MGI

neuron fate commitment

Inferred from mutant phenotype Ref.5. Source: MGI

oligodendrocyte differentiation

Inferred from mutant phenotype PubMed 16436615. Source: MGI

positive regulation of oligodendrocyte differentiation

Inferred from genetic interaction PubMed 11566099. Source: MGI

regulation of transcription, DNA-templated

Inferred from direct assay Ref.9. Source: MGI

spinal cord motor neuron differentiation

Inferred from mutant phenotype PubMed 21338882. Source: MGI

spinal cord oligodendrocyte cell differentiation

Inferred from genetic interaction PubMed 18287202. Source: MGI

spinal cord oligodendrocyte cell fate specification

Inferred from direct assay PubMed 18287202. Source: MGI

thalamus development

Inferred from mutant phenotype PubMed 19357274. Source: MGI

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15655114. Source: GOC

   Cellular_componentcytoplasm

Inferred from direct assay Ref.9. Source: MGI

nucleus

Inferred from direct assay Ref.9PubMed 20181579. Source: MGI

transcription factor complex

Inferred from sequence or structural similarity Ref.2. Source: MGI

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

HMG box domain binding

Inferred from physical interaction PubMed 16582099. Source: UniProtKB

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 15655114. Source: MGI

protein homodimerization activity

Inferred from physical interaction PubMed 15655114. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.9. Source: MGI

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Oligodendrocyte transcription factor 2
PRO_0000127415

Regions

Domain108 – 16255bHLH
Compositional bias77 – 8812Poly-Ser
Compositional bias206 – 2127Poly-Ala
Compositional bias225 – 23713Poly-Ala
Compositional bias266 – 2705Poly-Ala
Compositional bias273 – 28412Poly-Gly

Experimental info

Sequence conflict1981L → I in AAF61722. Ref.2
Sequence conflict2051P → PTRHGAP Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9EQW6 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 598AE76CB512D716

FASTA32332,407
        10         20         30         40         50         60 
MDSDASLVSS RPSSPEPDDL FLPARSKGGS SSGFTGGTVS SSTPSDCPPE LSSELRGAMG 

        70         80         90        100        110        120 
ASGAHPGDKL GGGGFKSSSS STSSSTSSAA TSSTKKDKKQ MTEPELQQLR LKINSRERKR 

       130        140        150        160        170        180 
MHDLNIAMDG LREVMPYAHG PSVRKLSKIA TLLLARNYIL MLTNSLEEMK RLVSEIYGGH 

       190        200        210        220        230        240 
HAGFHPSACG GLAHSAPLPT ATAHPAAAAH AAHHPAVHHP ILPPAAAAAA AAAAAAAVSS 

       250        260        270        280        290        300 
ASLPGSGLSS VGSIRPPHGL LKSPSAAAAA PLGGGGGGSG GSGGFQHWGG MPCPCSMCQV 

       310        320 
PPPHHHVSAM GAGTLPRLTS DAK 

« Hide

References

« Hide 'large scale' references
[1]"Dynamic expression of basic helix-loop-helix Olig family members: implication of Olig2 in neuron and oligodendrocyte differentiation and identification of a new member, Olig3."
Takebayashi H., Yoshida S., Sugimori M., Kosako H., Kominami R., Nakafuku M., Nabeshima Y.
Mech. Dev. 99:143-148(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: C57BL/6.
Tissue: Brain.
[2]"Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors."
Zhou Q., Wang S., Anderson D.J.
Neuron 25:331-343(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"Sonic hedgehog-regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system."
Lu Q.R., Yuk D.-I., Alberta J.A., Zhu Z., Pawlitzky I., Chan J.A., McMahon A.P., Stiles C.D., Rowitch D.H.
Neuron 25:317-329(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY SHH.
[5]"Common developmental requirement for Olig function indicates a motor neuron/oligodendrocyte connection."
Lu Q.R., Sun T., Zhu Z., Ma N., Garcia M., Stiles C.D., Rowitch D.H.
Cell 109:75-86(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"The basic helix-loop-helix factor olig2 is essential for the development of motoneuron and oligodendrocyte lineages."
Takebayashi H., Nabeshima Y., Yoshida S., Chisaka O., Ikenaka K., Nabeshima Y.
Curr. Biol. 12:1157-1163(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Non-overlapping expression of Olig3 and Olig2 in the embryonic neural tube."
Takebayashi H., Ohtsuki T., Uchida T., Kawamoto S., Okubo K., Ikenaka K., Takeichi M., Chisaka O., Nabeshima Y.
Mech. Dev. 113:169-174(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"Region-specific and stage-dependent regulation of Olig gene expression and oligodendrogenesis by Nkx6.1 homeodomain transcription factor."
Liu R., Cai J., Hu X., Tan M., Qi Y., German M., Rubenstein J.L.R., Sander M., Qiu M.
Development 130:6221-6231(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY NKX6-1.
[9]"Cross-repressive interaction of the Olig2 and Nkx2.2 transcription factors in developing neural tube associated with formation of a specific physical complex."
Sun T., Dong H., Wu L., Kane M., Rowitch D.H., Stiles C.D.
J. Neurosci. 23:9547-9556(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NKX2-2, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB038697 mRNA. Translation: BAB18907.1.
AF232929 Genomic DNA. Translation: AAF61722.1.
BC051967 mRNA. Translation: AAH51967.1.
RefSeqNP_058663.2. NM_016967.2.
UniGeneMm.37289.

3D structure databases

ProteinModelPortalQ9EQW6.
SMRQ9EQW6. Positions 110-166.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid206152. 7 interactions.
IntActQ9EQW6. 4 interactions.

PTM databases

PhosphoSiteQ9EQW6.

Proteomic databases

PRIDEQ9EQW6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000035608; ENSMUSP00000036797; ENSMUSG00000039830.
GeneID50913.
KEGGmmu:50913.
UCSCuc007zxe.1. mouse.

Organism-specific databases

CTD10215.
MGIMGI:1355331. Olig2.

Phylogenomic databases

eggNOGNOG313428.
GeneTreeENSGT00530000063239.
HOGENOMHOG000015765.
HOVERGENHBG053370.
InParanoidQ9EQW6.
KOK09085.
OMAKKSVHGF.
OrthoDBEOG7GQXWR.
PhylomeDBQ9EQW6.
TreeFamTF322733.

Gene expression databases

BgeeQ9EQW6.
CleanExMM_OLIG2.
GenevestigatorQ9EQW6.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
[Graphical view]
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio307903.
PROQ9EQW6.
SOURCESearch...

Entry information

Entry nameOLIG2_MOUSE
AccessionPrimary (citable) accession number: Q9EQW6
Secondary accession number(s): Q9JKN4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot