Oligodendrocyte transcription factor 2

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Q9EQW6 (OLIG2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 95. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Oligodendrocyte transcription factor 2
Short name=Oligo2

Gene names

Name:

Olig2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. Ref.5 Ref.6
Subunit structure	Interacts with NKX2-2. Ref.9
Subcellular location	Nucleus. Cytoplasm. Note: The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2. Ref.7 Ref.9
Tissue specificity	Expressed specifically in the brain. Ref.1
Developmental stage	Expressed in the ventral spinal cord as early as 9.5 dpc. Expression becomes progressively restricted to a narrow zone within the ventral neuroepithelium of the spinal cord. In the 14.5 dpc spinal cord, expressed in the oligodendrocyte progenitors of the ventral ventricular zone, but not dorsal root ganglia Schwann cells. Also expressed scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. In the brain, from 10.5 through 14.5 dpc, expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. By 15.5 dpc, dispersed throughout the gray matter, with little or no residual expression in the ventricular zone. In the postnatal brain, present preferentially in the white matter, such as corpus callosum and cerebellar medulla. Expressed in the 13.5 and 14.5 dpc retina and in the olfactory epithelium from 11.5 dpc onward. Ref.1 Ref.2
Induction	By SHH. Also induced by NKX6-1 in the developing spinal cord, but not in the rostral hindbrain. Ref.4 Ref.8
Domain	The bHLH is essential for interaction with NKX2-2.
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	myelination Inferred from mutant phenotype. Source: MGI negative regulation of neuron differentiation Inferred from direct assay. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI neuron fate commitment Inferred from mutant phenotype Ref.5. Source: MGI positive regulation of oligodendrocyte differentiation Inferred from genetic interaction. Source: MGI spinal cord motor neuron differentiation Inferred from mutant phenotype. Source: MGI spinal cord oligodendrocyte cell fate specification Inferred from direct assay. Source: MGI
Cellular component	cytoplasm Inferred from direct assay Ref.9. Source: MGI nucleus Inferred from direct assay Ref.9. Source: MGI
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW HMG box domain binding Inferred from physical interaction. Source: UniProtKB protein homodimerization activity Inferred from physical interaction. Source: MGI sequence-specific distal enhancer binding RNA polymerase II transcription factor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Id2	P41136	4	EBI-1213740,EBI-309167
Id4	P41139	5	EBI-1213740,EBI-1213725

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 323

323

Oligodendrocyte transcription factor 2

PRO_0000127415

Regions

Domain

121 – 162

Helix-loop-helix motif

DNA binding

109 – 120

Basic motif

Compositional bias

77 – 88

Poly-Ser

Compositional bias

206 – 212

Poly-Ala

Compositional bias

225 – 237

Poly-Ala

Compositional bias

266 – 270

Poly-Ala

Compositional bias

273 – 284

Poly-Gly

Experimental info

Sequence conflict

198

L → I in AAF61722. Ref.2

Sequence conflict

205

P → PTRHGAP Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q9EQW6 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 598AE76CB512D716
Show »

FASTA

323

32,407

        10         20         30         40         50         60 
MDSDASLVSS RPSSPEPDDL FLPARSKGGS SSGFTGGTVS SSTPSDCPPE LSSELRGAMG 

        70         80         90        100        110        120 
ASGAHPGDKL GGGGFKSSSS STSSSTSSAA TSSTKKDKKQ MTEPELQQLR LKINSRERKR 

       130        140        150        160        170        180 
MHDLNIAMDG LREVMPYAHG PSVRKLSKIA TLLLARNYIL MLTNSLEEMK RLVSEIYGGH 

       190        200        210        220        230        240 
HAGFHPSACG GLAHSAPLPT ATAHPAAAAH AAHHPAVHHP ILPPAAAAAA AAAAAAAVSS 

       250        260        270        280        290        300 
ASLPGSGLSS VGSIRPPHGL LKSPSAAAAA PLGGGGGGSG GSGGFQHWGG MPCPCSMCQV 

       310        320 
PPPHHHVSAM GAGTLPRLTS DAK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dynamic expression of basic helix-loop-helix Olig family members: implication of Olig2 in neuron and oligodendrocyte differentiation and identification of a new member, Olig3." Takebayashi H., Yoshida S., Sugimori M., Kosako H., Kominami R., Nakafuku M., Nabeshima Y. Mech. Dev. 99:143-148(2000) [PubMed: 11091082] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: C57BL/6. Tissue: Brain.
[2]	"Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors." Zhou Q., Wang S., Anderson D.J. Neuron 25:331-343(2000) [PubMed: 10719889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"Sonic hedgehog-regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system." Lu Q.R., Yuk D.-I., Alberta J.A., Zhu Z., Pawlitzky I., Chan J.A., McMahon A.P., Stiles C.D., Rowitch D.H. Neuron 25:317-329(2000) [PubMed: 10719888] [Abstract] Cited for: INDUCTION BY SHH.
[5]	"Common developmental requirement for Olig function indicates a motor neuron/oligodendrocyte connection." Lu Q.R., Sun T., Zhu Z., Ma N., Garcia M., Stiles C.D., Rowitch D.H. Cell 109:75-86(2002) [PubMed: 11955448] [Abstract] Cited for: FUNCTION.
[6]	"The basic helix-loop-helix factor olig2 is essential for the development of motoneuron and oligodendrocyte lineages." Takebayashi H., Nabeshima Y., Yoshida S., Chisaka O., Ikenaka K., Nabeshima Y. Curr. Biol. 12:1157-1163(2002) [PubMed: 12121626] [Abstract] Cited for: FUNCTION.
[7]	"Non-overlapping expression of Olig3 and Olig2 in the embryonic neural tube." Takebayashi H., Ohtsuki T., Uchida T., Kawamoto S., Okubo K., Ikenaka K., Takeichi M., Chisaka O., Nabeshima Y. Mech. Dev. 113:169-174(2002) [PubMed: 11960707] [Abstract] Cited for: SUBCELLULAR LOCATION.
[8]	"Region-specific and stage-dependent regulation of Olig gene expression and oligodendrogenesis by Nkx6.1 homeodomain transcription factor." Liu R., Cai J., Hu X., Tan M., Qi Y., German M., Rubenstein J.L.R., Sander M., Qiu M. Development 130:6221-6231(2003) [PubMed: 14602683] [Abstract] Cited for: INDUCTION BY NKX6-1.
[9]	"Cross-repressive interaction of the Olig2 and Nkx2.2 transcription factors in developing neural tube associated with formation of a specific physical complex." Sun T., Dong H., Wu L., Kane M., Rowitch D.H., Stiles C.D. J. Neurosci. 23:9547-9556(2003) [PubMed: 14573534] [Abstract] Cited for: INTERACTION WITH NKX2-2, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB038697 mRNA. Translation: BAB18907.1. AF232929 Genomic DNA. Translation: AAF61722.1. BC051967 mRNA. Translation: AAH51967.1.
IPI	IPI00226995.
RefSeq	NP_058663.2. NM_016967.2.
UniGene	Mm.37289.
3D structure databases
ProteinModelPortal	Q9EQW6.
SMR	Q9EQW6. Positions 109-167.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9EQW6. 4 interactions.
STRING	Q9EQW6.
PTM databases
PhosphoSite	Q9EQW6.
Proteomic databases
PRIDE	Q9EQW6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000035608; ENSMUSP00000036797; ENSMUSG00000039830.
GeneID	50913.
KEGG	mmu:50913.
Organism-specific databases
CTD	10215.
MGI	MGI:1355331. Olig2.
Phylogenomic databases
GeneTree	ENSGT00530000063239.
HOGENOM	HBG444019.
HOVERGEN	HBG053370.
InParanoid	Q9EQW6.
OMA	SFQHWGG.
OrthoDB	EOG4RJG2Q.
PhylomeDB	Q9EQW6.
Gene expression databases
ArrayExpress	Q9EQW6.
Bgee	Q9EQW6.
CleanEx	MM_OLIG2.
Genevestigator	Q9EQW6.
GermOnline	ENSMUSG00000039830. Mus musculus.
Family and domain databases
InterPro	IPR011598. HLH_DNA-bd. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
KO	K09085.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	307903.
SOURCE	Search...

Entry information

Entry name

OLIG2_MOUSE

Accession

Primary (citable) accession number: Q9EQW6
Secondary accession number(s): Q9JKN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 2002
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents