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Q9EQW6

- OLIG2_MOUSE

UniProt

Q9EQW6 - OLIG2_MOUSE

Protein

Oligodendrocyte transcription factor 2

Gene

Olig2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.2 Publications

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. HMG box domain binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. protein homodimerization activity Source: MGI
    5. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
    6. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. myelination Source: MGI
    2. negative regulation of neuron differentiation Source: MGI
    3. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    4. nervous system development Source: MGI
    5. neuron fate commitment Source: MGI
    6. oligodendrocyte differentiation Source: MGI
    7. positive regulation of oligodendrocyte differentiation Source: MGI
    8. regulation of transcription, DNA-templated Source: MGI
    9. spinal cord motor neuron differentiation Source: MGI
    10. spinal cord oligodendrocyte cell differentiation Source: MGI
    11. spinal cord oligodendrocyte cell fate specification Source: MGI
    12. thalamus development Source: MGI
    13. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Oligodendrocyte transcription factor 2
    Short name:
    Oligo2
    Gene namesi
    Name:Olig2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1355331. Olig2.

    Subcellular locationi

    Nucleus. Cytoplasm
    Note: The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: MGI
    3. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 323323Oligodendrocyte transcription factor 2PRO_0000127415Add
    BLAST

    Proteomic databases

    PRIDEiQ9EQW6.

    PTM databases

    PhosphoSiteiQ9EQW6.

    Expressioni

    Tissue specificityi

    Expressed specifically in the brain.1 Publication

    Developmental stagei

    Expressed in the ventral spinal cord as early as 9.5 dpc. Expression becomes progressively restricted to a narrow zone within the ventral neuroepithelium of the spinal cord. In the 14.5 dpc spinal cord, expressed in the oligodendrocyte progenitors of the ventral ventricular zone, but not dorsal root ganglia Schwann cells. Also expressed scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. In the brain, from 10.5 through 14.5 dpc, expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. By 15.5 dpc, dispersed throughout the gray matter, with little or no residual expression in the ventricular zone. In the postnatal brain, present preferentially in the white matter, such as corpus callosum and cerebellar medulla. Expressed in the 13.5 and 14.5 dpc retina and in the olfactory epithelium from 11.5 dpc onward.2 Publications

    Inductioni

    By SHH. Also induced by NKX6-1 in the developing spinal cord, but not in the rostral hindbrain.2 Publications

    Gene expression databases

    BgeeiQ9EQW6.
    CleanExiMM_OLIG2.
    GenevestigatoriQ9EQW6.

    Interactioni

    Subunit structurei

    Interacts with NKX2-2.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Id2P411364EBI-1213740,EBI-309167
    Id4P411395EBI-1213740,EBI-1213725

    Protein-protein interaction databases

    BioGridi206152. 7 interactions.
    IntActiQ9EQW6. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EQW6.
    SMRiQ9EQW6. Positions 110-166.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini108 – 16255bHLHPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi77 – 8812Poly-SerAdd
    BLAST
    Compositional biasi206 – 2127Poly-Ala
    Compositional biasi225 – 23713Poly-AlaAdd
    BLAST
    Compositional biasi266 – 2705Poly-Ala
    Compositional biasi273 – 28412Poly-GlyAdd
    BLAST

    Domaini

    The bHLH is essential for interaction with NKX2-2.

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG313428.
    GeneTreeiENSGT00530000063239.
    HOGENOMiHOG000015765.
    HOVERGENiHBG053370.
    InParanoidiQ9EQW6.
    KOiK09085.
    OMAiKKSVHGF.
    OrthoDBiEOG7GQXWR.
    PhylomeDBiQ9EQW6.
    TreeFamiTF322733.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    [Graphical view]
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9EQW6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSDASLVSS RPSSPEPDDL FLPARSKGGS SSGFTGGTVS SSTPSDCPPE    50
    LSSELRGAMG ASGAHPGDKL GGGGFKSSSS STSSSTSSAA TSSTKKDKKQ 100
    MTEPELQQLR LKINSRERKR MHDLNIAMDG LREVMPYAHG PSVRKLSKIA 150
    TLLLARNYIL MLTNSLEEMK RLVSEIYGGH HAGFHPSACG GLAHSAPLPT 200
    ATAHPAAAAH AAHHPAVHHP ILPPAAAAAA AAAAAAAVSS ASLPGSGLSS 250
    VGSIRPPHGL LKSPSAAAAA PLGGGGGGSG GSGGFQHWGG MPCPCSMCQV 300
    PPPHHHVSAM GAGTLPRLTS DAK 323
    Length:323
    Mass (Da):32,407
    Last modified:March 1, 2001 - v1
    Checksum:i598AE76CB512D716
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti198 – 1981L → I in AAF61722. (PubMed:10719889)Curated
    Sequence conflicti205 – 2051P → PTRHGAP(PubMed:10719889)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038697 mRNA. Translation: BAB18907.1.
    AF232929 Genomic DNA. Translation: AAF61722.1.
    BC051967 mRNA. Translation: AAH51967.1.
    CCDSiCCDS28323.1.
    RefSeqiNP_058663.2. NM_016967.2.
    UniGeneiMm.37289.

    Genome annotation databases

    EnsembliENSMUST00000035608; ENSMUSP00000036797; ENSMUSG00000039830.
    GeneIDi50913.
    KEGGimmu:50913.
    UCSCiuc007zxe.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038697 mRNA. Translation: BAB18907.1 .
    AF232929 Genomic DNA. Translation: AAF61722.1 .
    BC051967 mRNA. Translation: AAH51967.1 .
    CCDSi CCDS28323.1.
    RefSeqi NP_058663.2. NM_016967.2.
    UniGenei Mm.37289.

    3D structure databases

    ProteinModelPortali Q9EQW6.
    SMRi Q9EQW6. Positions 110-166.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 206152. 7 interactions.
    IntActi Q9EQW6. 4 interactions.

    PTM databases

    PhosphoSitei Q9EQW6.

    Proteomic databases

    PRIDEi Q9EQW6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035608 ; ENSMUSP00000036797 ; ENSMUSG00000039830 .
    GeneIDi 50913.
    KEGGi mmu:50913.
    UCSCi uc007zxe.1. mouse.

    Organism-specific databases

    CTDi 10215.
    MGIi MGI:1355331. Olig2.

    Phylogenomic databases

    eggNOGi NOG313428.
    GeneTreei ENSGT00530000063239.
    HOGENOMi HOG000015765.
    HOVERGENi HBG053370.
    InParanoidi Q9EQW6.
    KOi K09085.
    OMAi KKSVHGF.
    OrthoDBi EOG7GQXWR.
    PhylomeDBi Q9EQW6.
    TreeFami TF322733.

    Miscellaneous databases

    NextBioi 307903.
    PROi Q9EQW6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9EQW6.
    CleanExi MM_OLIG2.
    Genevestigatori Q9EQW6.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    [Graphical view ]
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dynamic expression of basic helix-loop-helix Olig family members: implication of Olig2 in neuron and oligodendrocyte differentiation and identification of a new member, Olig3."
      Takebayashi H., Yoshida S., Sugimori M., Kosako H., Kominami R., Nakafuku M., Nabeshima Y.
      Mech. Dev. 99:143-148(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: C57BL/6.
      Tissue: Brain.
    2. "Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors."
      Zhou Q., Wang S., Anderson D.J.
      Neuron 25:331-343(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Sonic hedgehog-regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system."
      Lu Q.R., Yuk D.-I., Alberta J.A., Zhu Z., Pawlitzky I., Chan J.A., McMahon A.P., Stiles C.D., Rowitch D.H.
      Neuron 25:317-329(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY SHH.
    5. "Common developmental requirement for Olig function indicates a motor neuron/oligodendrocyte connection."
      Lu Q.R., Sun T., Zhu Z., Ma N., Garcia M., Stiles C.D., Rowitch D.H.
      Cell 109:75-86(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "The basic helix-loop-helix factor olig2 is essential for the development of motoneuron and oligodendrocyte lineages."
      Takebayashi H., Nabeshima Y., Yoshida S., Chisaka O., Ikenaka K., Nabeshima Y.
      Curr. Biol. 12:1157-1163(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Non-overlapping expression of Olig3 and Olig2 in the embryonic neural tube."
      Takebayashi H., Ohtsuki T., Uchida T., Kawamoto S., Okubo K., Ikenaka K., Takeichi M., Chisaka O., Nabeshima Y.
      Mech. Dev. 113:169-174(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "Region-specific and stage-dependent regulation of Olig gene expression and oligodendrogenesis by Nkx6.1 homeodomain transcription factor."
      Liu R., Cai J., Hu X., Tan M., Qi Y., German M., Rubenstein J.L.R., Sander M., Qiu M.
      Development 130:6221-6231(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY NKX6-1.
    9. "Cross-repressive interaction of the Olig2 and Nkx2.2 transcription factors in developing neural tube associated with formation of a specific physical complex."
      Sun T., Dong H., Wu L., Kane M., Rowitch D.H., Stiles C.D.
      J. Neurosci. 23:9547-9556(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NKX2-2, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiOLIG2_MOUSE
    AccessioniPrimary (citable) accession number: Q9EQW6
    Secondary accession number(s): Q9JKN4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 2002
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3