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Q9EQW6

- OLIG2_MOUSE

UniProt

Q9EQW6 - OLIG2_MOUSE

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Protein
Oligodendrocyte transcription factor 2
Gene
Olig2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.2 Publications

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. HMG box domain binding Source: UniProtKB
  3. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
  4. protein binding Source: IntAct
  5. protein homodimerization activity Source: MGI
  6. sequence-specific DNA binding transcription factor activity Source: MGI
Complete GO annotation...

GO - Biological processi

  1. myelination Source: MGI
  2. negative regulation of neuron differentiation Source: MGI
  3. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  4. nervous system development Source: MGI
  5. neuron fate commitment Source: MGI
  6. oligodendrocyte differentiation Source: MGI
  7. positive regulation of oligodendrocyte differentiation Source: MGI
  8. regulation of transcription, DNA-templated Source: MGI
  9. spinal cord motor neuron differentiation Source: MGI
  10. spinal cord oligodendrocyte cell differentiation Source: MGI
  11. spinal cord oligodendrocyte cell fate specification Source: MGI
  12. thalamus development Source: MGI
  13. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Oligodendrocyte transcription factor 2
Short name:
Oligo2
Gene namesi
Name:Olig2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1355331. Olig2.

Subcellular locationi

Nucleus. Cytoplasm
Note: The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.2 Publications

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: MGI
  3. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Oligodendrocyte transcription factor 2
PRO_0000127415Add
BLAST

Proteomic databases

PRIDEiQ9EQW6.

PTM databases

PhosphoSiteiQ9EQW6.

Expressioni

Tissue specificityi

Expressed specifically in the brain.1 Publication

Developmental stagei

Expressed in the ventral spinal cord as early as 9.5 dpc. Expression becomes progressively restricted to a narrow zone within the ventral neuroepithelium of the spinal cord. In the 14.5 dpc spinal cord, expressed in the oligodendrocyte progenitors of the ventral ventricular zone, but not dorsal root ganglia Schwann cells. Also expressed scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. In the brain, from 10.5 through 14.5 dpc, expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. By 15.5 dpc, dispersed throughout the gray matter, with little or no residual expression in the ventricular zone. In the postnatal brain, present preferentially in the white matter, such as corpus callosum and cerebellar medulla. Expressed in the 13.5 and 14.5 dpc retina and in the olfactory epithelium from 11.5 dpc onward.2 Publications

Inductioni

By SHH. Also induced by NKX6-1 in the developing spinal cord, but not in the rostral hindbrain.2 Publications

Gene expression databases

BgeeiQ9EQW6.
CleanExiMM_OLIG2.
GenevestigatoriQ9EQW6.

Interactioni

Subunit structurei

Interacts with NKX2-2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Id2P411364EBI-1213740,EBI-309167
Id4P411395EBI-1213740,EBI-1213725

Protein-protein interaction databases

BioGridi206152. 7 interactions.
IntActiQ9EQW6. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9EQW6.
SMRiQ9EQW6. Positions 110-166.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 16255bHLH
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi77 – 8812Poly-Ser
Add
BLAST
Compositional biasi206 – 2127Poly-Ala
Compositional biasi225 – 23713Poly-Ala
Add
BLAST
Compositional biasi266 – 2705Poly-Ala
Compositional biasi273 – 28412Poly-Gly
Add
BLAST

Domaini

The bHLH is essential for interaction with NKX2-2.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG313428.
GeneTreeiENSGT00530000063239.
HOGENOMiHOG000015765.
HOVERGENiHBG053370.
InParanoidiQ9EQW6.
KOiK09085.
OMAiKKSVHGF.
OrthoDBiEOG7GQXWR.
PhylomeDBiQ9EQW6.
TreeFamiTF322733.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EQW6-1 [UniParc]FASTAAdd to Basket

« Hide

MDSDASLVSS RPSSPEPDDL FLPARSKGGS SSGFTGGTVS SSTPSDCPPE    50
LSSELRGAMG ASGAHPGDKL GGGGFKSSSS STSSSTSSAA TSSTKKDKKQ 100
MTEPELQQLR LKINSRERKR MHDLNIAMDG LREVMPYAHG PSVRKLSKIA 150
TLLLARNYIL MLTNSLEEMK RLVSEIYGGH HAGFHPSACG GLAHSAPLPT 200
ATAHPAAAAH AAHHPAVHHP ILPPAAAAAA AAAAAAAVSS ASLPGSGLSS 250
VGSIRPPHGL LKSPSAAAAA PLGGGGGGSG GSGGFQHWGG MPCPCSMCQV 300
PPPHHHVSAM GAGTLPRLTS DAK 323
Length:323
Mass (Da):32,407
Last modified:March 1, 2001 - v1
Checksum:i598AE76CB512D716
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti198 – 1981L → I in AAF61722. 1 Publication
Sequence conflicti205 – 2051P → PTRHGAP1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB038697 mRNA. Translation: BAB18907.1.
AF232929 Genomic DNA. Translation: AAF61722.1.
BC051967 mRNA. Translation: AAH51967.1.
CCDSiCCDS28323.1.
RefSeqiNP_058663.2. NM_016967.2.
UniGeneiMm.37289.

Genome annotation databases

EnsembliENSMUST00000035608; ENSMUSP00000036797; ENSMUSG00000039830.
GeneIDi50913.
KEGGimmu:50913.
UCSCiuc007zxe.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB038697 mRNA. Translation: BAB18907.1 .
AF232929 Genomic DNA. Translation: AAF61722.1 .
BC051967 mRNA. Translation: AAH51967.1 .
CCDSi CCDS28323.1.
RefSeqi NP_058663.2. NM_016967.2.
UniGenei Mm.37289.

3D structure databases

ProteinModelPortali Q9EQW6.
SMRi Q9EQW6. Positions 110-166.
ModBasei Search...

Protein-protein interaction databases

BioGridi 206152. 7 interactions.
IntActi Q9EQW6. 4 interactions.

PTM databases

PhosphoSitei Q9EQW6.

Proteomic databases

PRIDEi Q9EQW6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000035608 ; ENSMUSP00000036797 ; ENSMUSG00000039830 .
GeneIDi 50913.
KEGGi mmu:50913.
UCSCi uc007zxe.1. mouse.

Organism-specific databases

CTDi 10215.
MGIi MGI:1355331. Olig2.

Phylogenomic databases

eggNOGi NOG313428.
GeneTreei ENSGT00530000063239.
HOGENOMi HOG000015765.
HOVERGENi HBG053370.
InParanoidi Q9EQW6.
KOi K09085.
OMAi KKSVHGF.
OrthoDBi EOG7GQXWR.
PhylomeDBi Q9EQW6.
TreeFami TF322733.

Miscellaneous databases

NextBioi 307903.
PROi Q9EQW6.
SOURCEi Search...

Gene expression databases

Bgeei Q9EQW6.
CleanExi MM_OLIG2.
Genevestigatori Q9EQW6.

Family and domain databases

Gene3Di 4.10.280.10. 1 hit.
InterProi IPR011598. bHLH_dom.
[Graphical view ]
Pfami PF00010. HLH. 1 hit.
[Graphical view ]
SMARTi SM00353. HLH. 1 hit.
[Graphical view ]
SUPFAMi SSF47459. SSF47459. 1 hit.
PROSITEi PS50888. BHLH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Dynamic expression of basic helix-loop-helix Olig family members: implication of Olig2 in neuron and oligodendrocyte differentiation and identification of a new member, Olig3."
    Takebayashi H., Yoshida S., Sugimori M., Kosako H., Kominami R., Nakafuku M., Nabeshima Y.
    Mech. Dev. 99:143-148(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors."
    Zhou Q., Wang S., Anderson D.J.
    Neuron 25:331-343(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Sonic hedgehog-regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system."
    Lu Q.R., Yuk D.-I., Alberta J.A., Zhu Z., Pawlitzky I., Chan J.A., McMahon A.P., Stiles C.D., Rowitch D.H.
    Neuron 25:317-329(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY SHH.
  5. "Common developmental requirement for Olig function indicates a motor neuron/oligodendrocyte connection."
    Lu Q.R., Sun T., Zhu Z., Ma N., Garcia M., Stiles C.D., Rowitch D.H.
    Cell 109:75-86(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The basic helix-loop-helix factor olig2 is essential for the development of motoneuron and oligodendrocyte lineages."
    Takebayashi H., Nabeshima Y., Yoshida S., Chisaka O., Ikenaka K., Nabeshima Y.
    Curr. Biol. 12:1157-1163(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Non-overlapping expression of Olig3 and Olig2 in the embryonic neural tube."
    Takebayashi H., Ohtsuki T., Uchida T., Kawamoto S., Okubo K., Ikenaka K., Takeichi M., Chisaka O., Nabeshima Y.
    Mech. Dev. 113:169-174(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Region-specific and stage-dependent regulation of Olig gene expression and oligodendrogenesis by Nkx6.1 homeodomain transcription factor."
    Liu R., Cai J., Hu X., Tan M., Qi Y., German M., Rubenstein J.L.R., Sander M., Qiu M.
    Development 130:6221-6231(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY NKX6-1.
  9. "Cross-repressive interaction of the Olig2 and Nkx2.2 transcription factors in developing neural tube associated with formation of a specific physical complex."
    Sun T., Dong H., Wu L., Kane M., Rowitch D.H., Stiles C.D.
    J. Neurosci. 23:9547-9556(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NKX2-2, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiOLIG2_MOUSE
AccessioniPrimary (citable) accession number: Q9EQW6
Secondary accession number(s): Q9JKN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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