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Protein

Carboxypeptidase N catalytic chain

Gene

Cpn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.By similarity

Catalytic activityi

Release of a C-terminal basic amino acid, preferentially lysine.
Release of C-terminal arginine or lysine residues from polypeptides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi86Zinc; catalyticBy similarity1
Metal bindingi89Zinc; catalyticBy similarity1
Metal bindingi216Zinc; catalyticBy similarity1
Active sitei308Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • bradykinin catabolic process Source: RGD
  • protein processing Source: GO_Central
  • proteolysis Source: RGD
  • response to glucocorticoid Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase N catalytic chain (EC:3.4.17.3)
Short name:
CPN
Alternative name(s):
Carboxypeptidase N polypeptide 1
Carboxypeptidase N small subunit
Gene namesi
Name:Cpn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi70931. Cpn1.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000004257924 – 457Carboxypeptidase N catalytic chainAdd BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 104By similarity
Disulfide bondi271 ↔ 311By similarity
Glycosylationi400O-linked (GalNAc...)By similarity1
Glycosylationi402O-linked (GalNAc...)By similarity1
Glycosylationi409O-linked (GalNAc...)By similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9EQV8.
PRIDEiQ9EQV8.

Expressioni

Tissue specificityi

Plasma. Expressed in liver.1 Publication

Gene expression databases

BgeeiENSRNOG00000013439.
GenevisibleiQ9EQV8. RN.

Interactioni

Subunit structurei

Tetramer of two catalytic chains and two glycosylated inactive chains.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018221.

Structurei

3D structure databases

ProteinModelPortaliQ9EQV8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 340CatalyticBy similarityAdd BLAST320

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiQ9EQV8.
KOiK01292.
OMAiDTRIHIM.
OrthoDBiEOG091G06A9.
PhylomeDBiQ9EQV8.

Family and domain databases

Gene3Di2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR027063. CPN1.
IPR000834. Peptidase_M14.
[Graphical view]
PANTHERiPTHR11532:SF7. PTHR11532:SF7. 1 hit.
PfamiPF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EQV8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDLPSAFLP LLLLSKFVTP VTFRHHRYDD LVRTLYKVHN QCPDITRLYN
60 70 80 90 100
IGRSVKGRYL YVLEFSDYPG THEPLEPEVK YVGNMHGNEV LGRELLLQLS
110 120 130 140 150
EFLCEEFRNR NQRILRLIQD TRIHILPSMN PDGYEVAAAQ GPNTSGYLVG
160 170 180 190 200
RNNANGVDLN RNFPDLNTYF YYNSKYGGPN HHLPLPDNWK SQVEPETRAV
210 220 230 240 250
IQWIRSLNFV LSANMHGGAV VANYPYDKSL EHRFRSPHRT SNSPTPDDEL
260 270 280 290 300
FQTLAKVYSY AHGWMHQGWN CGDYFPDGIT NGASWYSLSK GMQDFNYLHT
310 320 330 340 350
NCFEITLELS CNKFPRQEEL QREWLGNREA LIQFLEQVHQ GIKGMVLDEN
360 370 380 390 400
YNNLTGAVIS VTGINHDVTS GEHGDYFRLL LPGTYSVTAK ASGYEPKTVT
410 420 430 440 450
VTVGPAGPTL VDFQLKRSTT QVHPVQKAPG RGQGSRAKQP RTSRKKDQAA

KRHRGPA
Length:457
Mass (Da):51,981
Last modified:March 1, 2001 - v1
Checksum:i6C8FC2C5325186D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042599 mRNA. Translation: BAB18618.1.
BC088124 mRNA. Translation: AAH88124.1.
RefSeqiNP_445978.1. NM_053526.2.
UniGeneiRn.145303.
Rn.162532.

Genome annotation databases

EnsembliENSRNOT00000018221; ENSRNOP00000018221; ENSRNOG00000013439.
GeneIDi365466.
KEGGirno:365466.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB042599 mRNA. Translation: BAB18618.1.
BC088124 mRNA. Translation: AAH88124.1.
RefSeqiNP_445978.1. NM_053526.2.
UniGeneiRn.145303.
Rn.162532.

3D structure databases

ProteinModelPortaliQ9EQV8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018221.

Proteomic databases

PaxDbiQ9EQV8.
PRIDEiQ9EQV8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018221; ENSRNOP00000018221; ENSRNOG00000013439.
GeneIDi365466.
KEGGirno:365466.

Organism-specific databases

CTDi1369.
RGDi70931. Cpn1.

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiQ9EQV8.
KOiK01292.
OMAiDTRIHIM.
OrthoDBiEOG091G06A9.
PhylomeDBiQ9EQV8.

Miscellaneous databases

PROiQ9EQV8.

Gene expression databases

BgeeiENSRNOG00000013439.
GenevisibleiQ9EQV8. RN.

Family and domain databases

Gene3Di2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR027063. CPN1.
IPR000834. Peptidase_M14.
[Graphical view]
PANTHERiPTHR11532:SF7. PTHR11532:SF7. 1 hit.
PfamiPF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBPN_RAT
AccessioniPrimary (citable) accession number: Q9EQV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: March 1, 2001
Last modified: October 5, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.