Q9EQU3 (TLR9_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 101.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Toll-like receptor 9 Alternative name(s): CD_antigen=CD289 | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 1032 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Plays a role in defense against systemic mouse cytomegalovirus infection. Controls lymphocyte response to Helicobacter infection. Ref.5 Ref.6 |
| Subunit structure | Interacts with MYD88 via their respective TIR domains By similarity. Interacts with BTK By similarity. Interacts with CNPY3. Interacts (via transmembrane domain) with UNC93B1. Ref.7 Ref.8 |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome. Lysosome. Cytoplasmic vesicle › phagosome. Note: Relocalizes from endoplasmic reticulum to endosome and lysosome upon stimulation with agonist. Ref.9 |
| Tissue specificity | Expressed in the basolateral region of gastric epithelial cells with high levels detected in antrum and body mucosa (at protein level). Detected in spleen and stomach at higher levels in C57BL/6 mice than BALB/C. Ref.5 |
| Induction | Following Helicobacter infection, down-regulated in C57BL/6 mice and up-regulated in BALB/C mice. Ref.5 |
| Disruption phenotype | Reduced proliferation of lymphocytes, reduced interferon-gamma production by splenocytes and reduced neutrophil numbers following Helicobacter infection. Ref.5 |
| Sequence similarities | Belongs to the Toll-like receptor family. Contains 26 LRR (leucine-rich) repeats. Contains 1 TIR domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 25 | 25 | Potential | ||||||
| Chain | 26 – 1032 | 1007 | Toll-like receptor 9 | PRO_0000034738 | |||||
Regions | |||||||||
| Topological domain | 26 – 818 | 793 | Extracellular Potential | ||||||
| Transmembrane | 819 – 839 | 21 | Helical; Potential | ||||||
| Topological domain | 840 – 1032 | 193 | Cytoplasmic Potential | ||||||
| Repeat | 62 – 85 | 24 | LRR 1 | ||||||
| Repeat | 87 – 110 | 24 | LRR 2 | ||||||
| Repeat | 122 – 147 | 26 | LRR 3 | ||||||
| Repeat | 150 – 166 | 17 | LRR 4 | ||||||
| Repeat | 167 – 190 | 24 | LRR 5 | ||||||
| Repeat | 198 – 221 | 24 | LRR 6 | ||||||
| Repeat | 223 – 242 | 20 | LRR 7 | ||||||
| Repeat | 243 – 268 | 26 | LRR 8 | ||||||
| Repeat | 283 – 306 | 24 | LRR 9 | ||||||
| Repeat | 308 – 332 | 25 | LRR 10 | ||||||
| Repeat | 333 – 356 | 24 | LRR 11 | ||||||
| Repeat | 363 – 386 | 24 | LRR 12 | ||||||
| Repeat | 390 – 413 | 24 | LRR 13 | ||||||
| Repeat | 415 – 440 | 26 | LRR 14 | ||||||
| Repeat | 471 – 495 | 25 | LRR 15 | ||||||
| Repeat | 497 – 520 | 24 | LRR 16 | ||||||
| Repeat | 521 – 544 | 24 | LRR 17 | ||||||
| Repeat | 546 – 573 | 28 | LRR 18 | ||||||
| Repeat | 575 – 599 | 25 | LRR 19 | ||||||
| Repeat | 601 – 623 | 23 | LRR 20 | ||||||
| Repeat | 628 – 651 | 24 | LRR 21 | ||||||
| Repeat | 653 – 676 | 24 | LRR 22 | ||||||
| Repeat | 677 – 700 | 24 | LRR 23 | ||||||
| Repeat | 702 – 724 | 23 | LRR 24 | ||||||
| Repeat | 725 – 748 | 24 | LRR 25 | ||||||
| Repeat | 750 – 773 | 24 | LRR 26 | ||||||
| Domain | 868 – 1016 | 149 | TIR | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 64 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 129 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 147 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 200 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 210 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 242 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 300 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 309 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 332 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 340 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 495 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 514 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 568 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 670 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 695 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 700 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 732 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 752 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Mutagenesis | 499 | 1 | L → P: Highly susceptible to mouse cytomegalovirus infection. Shows low level of cytokine induction and natural killer activation on viral infection. Ref.6 | ||||||
| Sequence conflict | 325 | 1 | T → N in BAB19260. Ref.1 | ||||||
| Sequence conflict | 325 | 1 | T → N in AAK29625. Ref.2 | ||||||
| Sequence conflict | 325 | 1 | T → N in AAK28488. Ref.3 | ||||||
| Sequence conflict | 325 | 1 | T → N in AAU04980. Ref.5 | ||||||
| Sequence conflict | 378 | 1 | L → S in BAB19260. Ref.1 | ||||||
| Sequence conflict | 378 | 1 | L → S in AAK29625. Ref.2 | ||||||
| Sequence conflict | 378 | 1 | L → S in AAK28488. Ref.3 | ||||||
| Sequence conflict | 378 | 1 | L → S in AAU04980. Ref.5 | ||||||
| Sequence conflict | 554 | 1 | S → G in AAK28488. Ref.3 | ||||||
| Sequence conflict | 562 | 1 | M → I in AAK28488. Ref.3 | ||||||
| Sequence conflict | 573 | 1 | T → A in AAK29625. Ref.2 | ||||||
| Sequence conflict | 573 | 1 | T → A in AAK28488. Ref.3 | ||||||
| Sequence conflict | 573 | 1 | T → A in AAU04980. Ref.5 | ||||||
| Sequence conflict | 579 | 1 | Q → H in AAK29625. Ref.2 | ||||||
| Sequence conflict | 579 | 1 | Q → H in AAK28488. Ref.3 | ||||||
| Sequence conflict | 579 | 1 | Q → H in AAU04980. Ref.5 | ||||||
| Sequence conflict | 867 | 1 | T → A in AAK29625. Ref.2 | ||||||
| Sequence conflict | 867 | 1 | T → A in AAK28488. Ref.3 | ||||||
| Sequence conflict | 867 | 1 | T → A in AAU04980. Ref.5 | ||||||
| Sequence conflict | 897 | 1 | E → G in AAK28488. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A Toll-like receptor recognizes bacterial DNA." Hemmi H., Takeuchi O., Kawai T., Kaisho T., Sato S., Sanjo H., Matsumoto M., Hoshino K., Wagner H., Takeda K., Akira S. Nature 408:740-745(2000) [PubMed: 11130078] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition." Bauer S.M., Kirschning C.J., Hacker H., Redecke V., Hausmann S., Akira S., Wagner H., Lipford G.B. Proc. Natl. Acad. Sci. U.S.A. 98:9237-9242(2001) [PubMed: 11470918] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Macrophage. |
| [3] | "Toll-like receptor 9 mediates CpG-DNA signaling." Chuang T.-H., Lee J., Kline L., Mathison J.C., Ulevitch R.J. J. Leukoc. Biol. 71:538-544(2002) [PubMed: 11867692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. |
| [4] | "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6. |
| [5] | "TLR9 polymorphisms determine murine lymphocyte responses to Helicobacter: results from a genome-wide scan." Anderson A.E., Worku M.L., Khamri W., Bamford K.B., Walker M.M., Thursz M.R. Eur. J. Immunol. 37:1548-1561(2007) [PubMed: 17474149] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-964, FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE. Strain: BALB/c and C57BL/6. |
| [6] | "Toll-like receptors 9 and 3 as essential components of innate immune defense against mouse cytomegalovirus infection." Tabeta K., Georgel P., Janssen E., Du X., Hoebe K., Crozat K., Mudd S., Shamel L., Sovath S., Goode J., Alexopoulou L., Flavell R.A., Beutler B. Proc. Natl. Acad. Sci. U.S.A. 101:3516-3521(2004) [PubMed: 14993594] [Abstract] Cited for: FUNCTION IN CYTOMEGALOVIRUS INFECTION, MUTAGENESIS OF LEU-499. |
| [7] | "The interaction between the ER membrane protein UNC93B and TLR3, 7, and 9 is crucial for TLR signaling." Brinkmann M.M., Spooner E., Hoebe K., Beutler B., Ploegh H.L., Kim Y.M. J. Cell Biol. 177:265-275(2007) [PubMed: 17452530] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH UNC93B1. |
| [8] | "A single base mutation in the PRAT4A gene reveals differential interaction of PRAT4A with Toll-like receptors." Kiyokawa T., Akashi-Takamura S., Shibata T., Matsumoto F., Nishitani C., Kuroki Y., Seto Y., Miyake K. Int. Immunol. 20:1407-1415(2008) [PubMed: 18780723] [Abstract] Cited for: INTERACTION WITH CNPY3. |
| [9] | "UNC93B1 delivers nucleotide-sensing toll-like receptors to endolysosomes." Kim Y.M., Brinkmann M.M., Paquet M.E., Ploegh H.L. Nature 452:234-238(2008) [PubMed: 18305481] [Abstract] Cited for: SUBCELLULAR LOCATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB045181 mRNA. Translation: BAB19260.1. AF348140 mRNA. Translation: AAK29625.1. AF314224 mRNA. Translation: AAK28488.1. AC164430 Genomic DNA. No translation available. AY649790 Genomic DNA. Translation: AAU04980.1. AY649791 Genomic DNA. Translation: AAU04981.1. |
| IPI | IPI00318748. IPI01027735. |
| RefSeq | NP_112455.2. NM_031178.2. |
| UniGene | Mm.44889. |
3D structure databases | |
| ProteinModelPortal | Q9EQU3. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9EQU3. |
Proteomic databases | |
| PRIDE | Q9EQU3. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000062241; ENSMUSP00000082207; ENSMUSG00000045322. |
| GeneID | 81897. |
| KEGG | mmu:81897. |
Organism-specific databases | |
| CTD | 54106. |
| MGI | MGI:1932389. Tlr9. |
Phylogenomic databases | |
| GeneTree | ENSGT00600000084070. |
| HOGENOM | HBG447035. |
| HOVERGEN | HBG018601. |
| InParanoid | Q9EQU3. |
| OrthoDB | EOG47SSDD. |
Gene expression databases | |
| ArrayExpress | Q9EQU3. |
| Bgee | Q9EQU3. |
| CleanEx | MM_TLR9. |
| Genevestigator | Q9EQU3. |
| GermOnline | ENSMUSG00000045322. Mus musculus. |
Family and domain databases | |
| InterPro | IPR001611. Leu-rich_rpt. IPR003591. Leu-rich_rpt_typical-subtyp. IPR000157. TIR_dom. [Graphical view] |
| KO | K10161. |
| Pfam | PF00560. LRR_1. 3 hits. PF01582. TIR. 1 hit. [Graphical view] |
| SMART | SM00369. LRR_TYP. 1 hit. [Graphical view] |
| SUPFAM | SSF52200. TIR. 1 hit. |
| PROSITE | PS51450. LRR. 16 hits. PS50104. TIR. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| SOURCE | Search... |
Entry information
| Entry name | TLR9_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q9EQU3 Secondary accession number(s): F8VPN5 Q99MQ8 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with